LOCUS pTK105 10707 bp DNA circular UNA 01-AUG-2014 DEFINITION SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn EYFP-PEST. ACCESSION urn.local.tasuku.1377628464201.6001 VERSION urn.local.tasuku.1377628464201.6001 KEYWORDS . SOURCE Sindbis ORGANISM Sindbis . FEATURES Location/Qualifiers 5'UTR 1..59 CDS 60..1679 /label="nsP1" CDS 1680..4100 /label="nsP2" primer_bind 2517..2538 /label="TK-SIN-ClaI-ncp-seq-F1" misc_feature 2992 /label="C" /note="Geneious type: Editing History Replacement" misc_feature 3579 /label="A" /note="Geneious type: Editing History Replacement" Mutation 3855 /label="P726S ncp mutation" CDS 4101..5747 /label="nsP3" primer_bind complement(4443..4462) /label="TK-SIN-AvrII-ncp-seq-R1" Stop_codon 5748..5750 /label="Opal stop codon" CDS 5751..7601 /label="nsP4" primer_bind 6086..6105 /label="TK-SIN-ts-seq-F1" primer_bind 7103..7126 /label="TK-nsP4-SapI-F" primer_bind 7468..7487 /label="TK-nsP4-seq-F2" promoter 7500..7611 /label="Subgenomic promoter (98/14)" promoter 7579..7611 /label="Subgenomic promoter (19/14)" primer_bind 7587..7656 /label="TK-14-2K-F1" primer_bind complement(7587..7656) /label="TK-14-2K-R1" misc_feature 7598..7611 /label="Subgenomic RNA 5' UTR" primer_bind 7598..7641 /label="TK-T7-SGP14-K-turn-F" Aptamer 7612..7634 /label="BoxCD mini G/C" Aptamer 7642..7664 /label="BoxCD mini G/C" primer_bind 7642..7701 /label="TK-2K-EYFP-F" primer_bind complement(7642..7701) /label="TK-2K-EYFP-R" misc_feature 7678..7683 /label="Kozak" CDS 7684..8400 /label="EYFP" primer_bind complement(7956..7975) /label="TK-EYFP-seq-R" CDS 8401..8526 /label="PEST" primer_bind complement(8503..8545) /label="TK-3'UTR-XhoI-PEST-R" 3'UTR 8530..8751 misc_feature 8560 /label /note="Geneious type: Editing History Insertion" primer_bind complement(8597..8619) /label="TK-SIN-3'UTR-seq-R" misc_feature 8751 /label="A" /note="Geneious type: Editing History Replacement" primer_bind complement(8751..8774) /label="TK-SIN-3'UTR-R" CDS 8983..9840 /label="AmpR" promoter 10690..10707 /label="SP6" ORIGIN 1 attgacggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa 61 tggagaagcc agtagtaaac gtagacgtag acccccagag tccgtttgtc gtgcaactgc 121 aaaaaagctt cccgcaattt gaggtagtag cacagcaggt cactccaaat gaccatgcta 181 atgccagagc attttcgcat ctggccagta aactaatcga gctggaggtt cctaccacag 241 cgacgatctt ggacataggc agcgcaccgg ctcgtagaat gttttccgag caccagtatc 301 attgtgtctg ccccatgcgt agtccagaag acccggaccg catgatgaaa tacgccagta 361 aactggcgga aaaagcgtgc aagattacaa acaagaactt gcatgagaag attaaggatc 421 tccggaccgt acttgatacg ccggatgctg aaacaccatc gctctgcttt cacaacgatg 481 ttacctgcaa catgcgtgcc gaatattccg tcatgcagga cgtgtatatc aacgctcccg 541 gaactatcta tcatcaggct atgaaaggcg tgcggaccct gtactggatt ggcttcgaca 601 ccacccagtt catgttctcg gctatggcag gttcgtaccc tgcgtacaac accaactggg 661 ccgacgagaa agtccttgaa gcgcgtaaca tcggactttg cagcacaaag ctgagtgaag 721 gtaggacagg aaaattgtcg ataatgagga agaaggagtt gaagcccggg tcgcgggttt 781 atttctccgt aggatcgaca ctttatccag aacacagagc cagcttgcag agctggcatc 841 ttccatcggt gttccacttg aatggaaagc agtcgtacac ttgccgctgt gatacagtgg 901 tgagttgcga aggctacgta gtgaagaaaa tcaccatcag tcccgggatc acgggagaaa 961 ccgtgggata cgcggttaca cacaatagcg agggcttctt gctatgcaaa gttactgaca 1021 cagtaaaagg agaacgggta tcgttccctg tgtgcacgta catcccggcc accatatgcg 1081 atcagatgac tggtataatg gccacggata tatcacctga cgatgcacaa aaacttctgg 1141 ttgggctcaa ccagcgaatt gtcattaacg gtaggactaa caggaacacc aacaccatgc 1201 aaaattacct tctgccgatc atagcacaag ggttcagcaa atgggctaag gagcgcaagg 1261 atgatcttga taacgagaaa atgctgggta ctagagaacg caagcttacg tatggctgct 1321 tgtgggcgtt tcgcactaag aaagtacatt cgttttatcg cccacctgga acgcagacct 1381 gcgtaaaagt cccagcctct tttagcgctt ttcccatgtc gtccgtatgg acgacctctt 1441 tgcccatgtc gctgaggcag aaattgaaac tggcattgca accaaagaag gaggaaaaac 1501 tgctgcaggt ctcggaggaa ttagtcatgg aggccaaggc tgcttttgag gatgctcagg 1561 aggaagccag agcggagaag ctccgagaag cacttccacc attagtggca gacaaaggca 1621 tcgaggcagc cgcagaagtt gtctgcgaag tggaggggct ccaggcggac atcggagcag 1681 cattagttga aaccccgcgc ggtcacgtaa ggataatacc tcaagcaaat gaccgtatga 1741 tcggacagta tatcgttgtc tcgccaaact ctgtgctgaa gaatgccaaa ctcgcaccag 1801 cgcacccgct agcagatcag gttaagatca taacacactc cggaagatca ggaaggtacg 1861 cggtcgaacc atacgacgct aaagtactga tgccagcagg aggtgccgta ccatggccag 1921 aattcctagc actgagtgag agcgccacgt tagtgtacaa cgaaagagag tttgtgaacc 1981 gcaaactata ccacattgcc atgcatggcc ccgccaagaa tacagaagag gagcagtaca 2041 aggttacaaa ggcagagctt gcagaaacag agtacgtgtt tgacgtggac aagaagcgtt 2101 gcgttaagaa ggaagaagcc tcaggtctgg tcctctcggg agaactgacc aaccctccct 2161 atcatgagct agctctggag ggactgaaga cccgacctgc ggtcccgtac aaggtcgaaa 2221 caataggagt gataggcaca ccggggtcgg gcaagtcagc tattatcaag tcaactgtca 2281 cggcacgaga tcttgttacc agcggaaaga aagaaaattg tcgcgaaatt gaggccgacg 2341 tgctaagact gaggggtatg cagattacgt cgaagacagt agattcggtt atgctcaacg 2401 gatgccacaa agccgtagaa gtgctgtacg ttgacgaagc gttcgcgtgc cacgcaggag 2461 cactacttgc cttgattgct atcgtcaggc cccgcaagaa ggtagtacta tgcggagacc 2521 ccatgcaatg cggattcttc aacatgatgc aactaaaggt acatttcaat caccctgaaa 2581 aagacatatg caccaagaca ttctacaagt atatctcccg gcgttgcaca cagccagtta 2641 cagctattgt atcgacactg cattacgatg gaaagatgaa aaccacgaac ccgtgcaaga 2701 agaacattga aatcgatatt acaggggcca caaagccgaa gccaggggat atcatcctga 2761 catgtttccg cgggtgggtt aagcaattgc aaatcgacta tcccggacat gaagtaatga 2821 cagccgcggc ctcacaaggg ctaaccagaa aaggagtgta tgccgtccgg caaaaagtca 2881 atgaaaaccc actgtacgcg atcacatcag agcatgtgaa cgtgttgctc acccgcactg 2941 aggacaggct agtgtggaaa accttgcagg gcgacccatg gattaagcag ctcactaaca 3001 tacctaaagg aaactttcag gctactatag aggactggga agctgaacac aagggaataa 3061 ttgctgcaat aaacagcccc actccccgtg ccaatccgtt cagctgcaag accaacgttt 3121 gctgggcgaa agcattggaa ccgatactag ccacggccgg tatcgtactt accggttgcc 3181 agtggagcga actgttccca cagtttgcgg atgacaaacc acattcggcc atttacgcct 3241 tagacgtaat ttgcattaag tttttcggca tggacttgac aagcggactg ttttctaaac 3301 agagcatccc actaacgtac catcccgccg attcagcgag gccggtagct cattgggaca 3361 acagcccagg aacccgcaag tatgggtacg atcacgccat tgccgccgaa ctctcccgta 3421 gatttccggt gttccagcta gctgggaagg gcacacaact tgatttgcag acggggagaa 3481 ccagagttat ctctgcacag cataacctgg tcccggtgaa ccgcaatctt cctcacgcct 3541 tagtccccga gtacaaggag aagcaacccg gcccggtcga aaaattcttg aaccagttca 3601 aacaccactc agtacttgtg gtatcagagg aaaaaattga agctccccgt aagagaatcg 3661 aatggatcgc cccgattggc atagccggtg cagataagaa ctacaacctg gctttcgggt 3721 ttccgccgca ggcacggtac gacctggtgt tcatcaacat tggaactaaa tacagaaacc 3781 accactttca gcagtgcgaa gaccatgcgg cgaccttaaa aaccctttcg cgttcggccc 3841 tgaattgcct taactcagga ggcaccctcg tggtgaagtc ctatggctac gccgaccgca 3901 acagtgagga cgtagtcacc gctcttgcca gaaagtttgt cagggtgtct gcagcgagac 3961 cagattgtgt ctcaagcaat acagaaatgt acctgatttt ccgacaacta gacaacagcc 4021 gtacacggca attcaccccg caccatctga attgcgtgat ttcgtccgtg tatgagggta 4081 caagagatgg agttggagcc gcgccgtcat accgcaccaa aagggagaat attgctgact 4141 gtcaagagga agcagttgtc aacgcagcca atccgctggg tagaccaggc gaaggagtct 4201 gccgtgccat ctataaacgt tggccgacca gttttaccga ttcagccacg gagacaggca 4261 ccgcaagaat gactgtgtgc ctaggaaaga aagtgatcca cgcggtcggc cctgatttcc 4321 ggaagcaccc agaagcagaa gccttgaaat tgctacaaaa cgcctaccat gcagtggcag 4381 acttagtaaa tgaacataac atcaagtctg tcgccattcc actgctatct acaggcattt 4441 acgcagccgg aaaagaccgc cttgaagtat cacttaactg cttgacaacc gcgctagaca 4501 gaactgacgc ggacgtaacc atctattgcc tggataagaa gtggaaggaa agaatcgacg 4561 cggcactcca acttaaggag tctgtaacag agctgaagga tgaagatatg gagatcgacg 4621 atgagttagt atggatccat ccagacagtt gcttgaaggg aagaaaggga ttcagtacta 4681 caaaaggaaa attgtattcg tacttcgaag gcaccaaatt ccatcaagca gcaaaagaca 4741 tggcggagat aaaggtcctg ttccctaatg accaggaaag taatgaacaa ctgtgtgcct 4801 acatattggg tgagaccatg gaagcaatcc gcgaaaagtg cccggtcgac cataacccgt 4861 cgtctagccc gcccaaaacg ttgccgtgcc tttgcatgta tgccatgacg ccagaaaggg 4921 tccacagact tagaagcaat aacgtcaaag aagttacagt atgctcctcc accccccttc 4981 ctaagcacaa aattaagaat gttcagaagg ttcagtgcac gaaagtagtc ctgtttaatc 5041 cgcacactcc cgcattcgtt cccgcccgta agtacataga agtgccagaa cagcctaccg 5101 ctcctcctgc acaggccgag gaggcccccg aagttgtagc gacaccgtca ccatctacag 5161 ctgataacac ctcgcttgat gtcacagaca tctcactgga tatggatgac agtagcgaag 5221 gctcactttt ttcgagcttt agcggatcgg acaactctat tactagtatg gacagttggt 5281 cgtcaggacc tagttcacta gagatagtag accgaaggca ggtggtggtg gctgacgttc 5341 atgccgtcca agagcctgcc cctattccac cgccaaggct aaagaagatg gcccgcctgg 5401 cagcggcaag aaaagagccc actccaccgg caagcaatag ctctgagtcc ctccacctct 5461 cttttggtgg ggtatccatg tccctcggat caattttcga cggagagacg gcccgccagg 5521 cagcggtaca acccctggca acaggcccca cggatgtgcc tatgtctttc ggatcgtttt 5581 ccgacggaga gattgatgag ctgagccgca gagtaactga gtccgaaccc gtcctgtttg 5641 gatcatttga accgggcgaa gtgaactcaa ttatatcgtc ccgatcagcc gtatcttttc 5701 cactacgcaa gcagagacgt agacgcagga gcaggaggac tgaatactga ctaaccgggg 5761 taggtgggta catattttcg acggacacag gccctgggca cttgcaaaag aagtccgttc 5821 tgcagaacca gcttacagaa ccgaccttgg agcgcaatgt cctggaaaga attcatgccc 5881 cggtgctcga cacgtcgaaa gaggaacaac tcaaactcag gtaccagatg atgcccaccg 5941 aagccaacaa aagtaggtac cagtctcgta aagtagaaaa tcagaaagcc ataaccactg 6001 agcgactact gtcaggacta cgactgtata actctgccac agatcagcca gaatgctata 6061 agatcaccta tccgaaacca ttgtactcca gtagcgtacc ggcgaactac tccgatccac 6121 agttcgctgt agctgtctgt aacaactatc tgcatgagaa ctatccgaca gtagcatctt 6181 atcagattac tgacgagtac gatgcttact tggatatggt agacgggaca gtcgcctgcc 6241 tggatactgc aaccttctgc cccgctaagc ttagaagtta cccgaaaaaa catgagtata 6301 gagccccgaa tatccgcagt gcggttccat cagcgatgca gaacacgcta caaaatgtgc 6361 tcattgccgc aactaaaaga aattgcaacg tcacgcagat gcgtgaactg ccaacactgg 6421 actcagcgac attcaatgtc gaatgctttc gaaaatatgc atgtaatgac gagtattggg 6481 aggagttcgc tcggaagcca attaggatta ccactgagtt tgtcaccgca tatgtagcta 6541 gactgaaagg ccctaaggcc gccgcactat ttgcaaagac gtataatttg gtcccattgc 6601 aagaagtgcc tatggataga ttcgtcatgg acatgaaaag agacgtgaaa gttacaccag 6661 gcacgaaaca cacagaagaa agaccgaaag tacaagtgat acaagccgca gaacccctgg 6721 cgactgctta cttatgcggg attcaccggg aattagtgcg taggcttacg gccgtcttgc 6781 ttccaaacat tcacacgctt tttgacatgt cggcggagga ttttgatgca atcatagcag 6841 aacacttcaa gcaaggcgac ccggtactgg agacggatat cgcatcattc gacaaaagcc 6901 aagacgacgc tatggcgtta accggtctga tgatcttgga ggacctgggt gtggatcaac 6961 cactactcga cttgatcgag tgcgcctttg gagaaatatc atccacccat ctacctacgg 7021 gtactcgttt taaattcggg gcgatgatga aatccggaat gttcctcaca ctttttgtca 7081 acacagtttt gaatgtcgtt atcgccagca gagtactaga agagcggctt aaaacgtcca 7141 gatgtgcagc gttcattggc gacgacaaca tcatacatgg agtagtatct gacaaagaaa 7201 tggctgagag gtgcgccacc tggctcaaca tggaggttaa gatcatcgac gcagtcatcg 7261 gtgagagacc accttacttc tgcggcggat ttatcttgca agattcggtt acttccacag 7321 cgtgccgcgt ggcggatccc ctgaaaaggc tgtttaagtt gggtaaaccg ctcccagccg 7381 acgacgagca agacgaagac agaagacgcg ctctgctaga tgaaacaaag gcgtggttta 7441 gagtaggtat aacaggcact ttagcagtgg ccgtgacgac ccggtatgag gtagacaata 7501 ttacacctgt cctactggca ttgagaactt ttgcccagag caaaagagca ttccaagcca 7561 tcagagggga aataaagcat ctctacggtg gtcctaaata gtcagcatag tgggcgtgat 7621 ccgaaaggtg acccggatct ggggcgtgat ccgaaaggtg acccggatcc accggtcgcc 7681 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 7741 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 7801 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 7861 accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg 7921 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 7981 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 8041 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 8101 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 8161 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 8221 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 8281 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 8341 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 8401 aagcttagcc atggcttccc gccggaggtg gaggagcagg atgatggcac gctgcccatg 8461 tcttgtgccc aggagagcgg gatggaccgt caccctgcag cctgtgcttc tgctaggatc 8521 aatgtgtaac tcgaggaagc gcagtacata atgctgcgca gtgttgccac ataaccacta 8581 tattaaccat ttatctagcg gacgccaaaa actcaatgta tttctgagga agcgtggtgc 8641 ataatgccac gcagcgtctg cataactttt attatttctt ttattaatca acaaaatttt 8701 gtttttaaca tttcaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ggggaattcg 8761 agctcgggaa ttaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt 8821 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc 8881 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 8941 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 9001 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 9061 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 9121 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 9181 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa 9241 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 9301 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 9361 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 9421 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 9481 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 9541 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 9601 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 9661 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 9721 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 9781 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 9841 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 9901 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 9961 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 10021 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 10081 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 10141 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 10201 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 10261 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 10321 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 10381 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag 10441 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 10501 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 10561 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcgagc 10621 tcgtatggac atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat 10681 acacatacga tttaggtgac actatag //