LOCUS       pTK313                 10712 bp    DNA     circular UNA 31-JUL-2014
DEFINITION  TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak
            EGFP-PEST ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI.
ACCESSION   urn.local.tasuku.1396367570783.93
VERSION     urn.local.tasuku.1396367570783.93
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     5'UTR           1..44
     Mutation        3
                     /label="A->G to create Frolov's """"5' VEE"""" version of
                     TC-83"
     CDS             45..1649
                     /product="nsP1"
                     /note="putative"
                     /label="nsP1"
     misc_feature    106..107
                     /note="this correction results in changing amino acid
                     nsP1-21-Thr to nsP1-21-Ser, this 1992 data corrects the
                     sequence reported in the reference"
                     /citation="[1]; [2]"
                     /replace="gc"
                     /label="Corrected sequence"
     misc_feature    134..184
                     /label="51 nucleotide conserved sequence"
     primer_bind     286..305
                     /label="1F"
     primer_bind     complement(510..529)
                     /label="1R"
     primer_bind     1188..1207
                     /label="2F"
     primer_bind     complement(1391..1410)
                     /label="2R"
     old_sequence    1533..1534
                     /note="this correction changes amino acid nsP1-497-Arg to
                     nsP1-497-Ala, this data corrects the sequence reported in
                     the reference"
                     /citation="[1]; [2]"
                     /replace="gc"
     CDS             1650..4031
                     /product="nsP2"
                     /function="replication"
                     /note="putative"
                     /label="nsP2"
     Mutation        1696
                     /label="TRD/TC-83 discrepency"
     primer_bind     2055..2074
                     /label="3F"
     primer_bind     complement(2262..2281)
                     /label="3R"
     primer_bind     2874..2893
                     /label="4F"
     primer_bind     complement(3110..3129)
                     /label="4R"
     primer_bind     3771..3790
                     /label="5F"
     Mutation        3864..3866
                     /label="Q739L noncytopathic mutation (CAG->CTG)"
     misc_feature    3865
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(4024..4043)
                     /label="5R"
     CDS             4032..5702
                     /product="nsP3"
                     /function="replication"
                     /note="putative"
                     /label="nsP3"
     primer_bind     4600..4619
                     /label="6F"
     primer_bind     complement(4853..4872)
                     /label="6R"
     primer_bind     5489..5508
                     /label="7F"
     CDS             5703..7523
                     /product="nsP4"
                     /function="replication"
                     /note="putative"
                     /label="nsP4"
     primer_bind     complement(5797..5816)
                     /label="7R"
     primer_bind     6425..6446
                     /label="8F"
     primer_bind     complement(6663..6682)
                     /label="8R"
     primer_bind     7303..7322
                     /label="9F"
     primer_bind     7369..7389
                     /label="TK-VEE-nsP4-EGFP-IDT-qPCR-F2"
     gBlock          7369..7693
                     /label="TK-VEE-nsP4-EGFP-IDT"
     primer_bind     7426..7447
                     /label="TK-VEE-nsP4-EGFP-IDT-qPCR-F1"
     primer_bind     7451..7472
                     /label="TK-VEE-nsP4-seq-F"
     primer_bind     7499..7518
                     /label="TK-VEE-nsP4-EGFP/mKate-qPCR-F"
     misc_feature    7532..7561
                     /label="Subgenomic RNA 5'UTR"
     misc_feature    7562..7567
                     /label="XbaI site"
     attB1           7568..7592
     misc_feature    7576..>7579
                     /label="attB1 x attP1 core recombination site"
     misc_feature    <7580..7582
                     /label="attB1 x attP1 core recombination site"
     misc_RNA        7593..7598
                     /label="Kozak"
     CDS             7599..8315
                     /label="EGFP"
     primer_bind     complement(7664..7683)
                     /label="TK-VEE-nsP4-EGFP-qPCR-R"
     primer_bind     complement(7665..7684)
                     /label="TK-VEE-nsP4-EGFP-IDT-qPCR-R1"
     primer_bind     complement(7677..7693)
                     /label="TK-VEE-nsP4-EGFP-IDT-qPCR-R2"
     CDS             8316..8441
                     /label="PEST"
     attB2           complement(8445..8469)
     misc_feature    complement(8455..8461)
                     /label="attB2 x attP2 core recombination site"
     misc_feature    8470..8477
                     /label="AscI site"
     CDS             <8478..8687
                     /label="E1 (truncated)"
     primer_bind     complement(8548..8567)
                     /label="TK-VEE-E1-seq-R"
     3'UTR           8691..8807
     primer_bind     complement(8738..8760)
                     /label="TK-VEE-3'UTR-seq-R"
     misc_feature    8789..8807
                     /label="19 nucleotide conserved sequence"
     misc_RNA        8808..8847
                     /label="Poly A"
     misc_feature    8848..8865
                     /label="I-SceI site"
     rep_origin      complement(8922..9510)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
     misc_RNA        complement(8922..9474)
                     /note="RNAII transcript (complementary strand)"
     primer_bind     complement(9270..9289)
                     /label="RepO-R"
     -35_signal      9328..9333
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      9350..9355
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        9364..9471
                     /note="RNAI transcript"
     -10_signal      complement(9484..9489)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(9505..9510)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     gene            complement(9681..10541)
                     /gene="bla"
     CDS             complement(9681..10541)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     primer_bind     10304..10323
                     /label="Amp-R"
     sig_peptide     complement(10473..10541)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
     -10_signal      complement(10585..10590)
                     /note="bla promoter (counter-clockwise); GAGACA"
     -35_signal      complement(10606..10611)
                     /note="bla promoter (counter-clockwise); TTCAAA"
     misc_feature    10677..10694
                     /label="I-SceI site"
     promoter        10695..10712
                     /label="T7 promoter"
     misc_feature    10712..44
                     /label="T7 transcript start"
     misc_feature    10712..1
                     /Note="GG would increase txn 2-fold but viral sequence
                     starts with A"
                     /label="Promoter note"
ORIGIN      
        1 atgggcggcg catgagagaa gcccagacca attacctacc caaaatggag aaagttcacg
       61 ttgacatcga ggaagacagc ccattcctca gagctttgca gcggagcttc ccgcagtttg
      121 aggtagaagc caagcaggtc actgataatg accatgctaa tgccagagcg ttttcgcatc
      181 tggcttcaaa actgatcgaa acggaggtgg acccatccga cacgatcctt gacattggaa
      241 gtgcgcccgc ccgcagaatg tattctaagc acaagtatca ttgtatctgt ccgatgagat
      301 gtgcggaaga tccggacaga ttgtataagt atgcaactaa gctgaagaaa aactgtaagg
      361 aaataactga taaggaattg gacaagaaaa tgaaggagct cgccgccgtc atgagcgacc
      421 ctgacctgga aactgagact atgtgcctcc acgacgacga gtcgtgtcgc tacgaagggc
      481 aagtcgctgt ttaccaggat gtatacgcgg ttgacggacc gacaagtctc tatcaccaag
      541 ccaataaggg agttagagtc gcctactgga taggctttga caccacccct tttatgttta
      601 agaacttggc tggagcatat ccatcatact ctaccaactg ggccgacgaa accgtgttaa
      661 cggctcgtaa cataggccta tgcagctctg acgttatgga gcggtcacgt agagggatgt
      721 ccattcttag aaagaagtat ttgaaaccat ccaacaatgt tctattctct gttggctcga
      781 ccatctacca cgagaagagg gacttactga ggagctggca cctgccgtct gtatttcact
      841 tacgtggcaa gcaaaattac acatgtcggt gtgagactat agttagttgc gacgggtacg
      901 tcgttaaaag aatagctatc agtccaggcc tgtatgggaa gccttcaggc tatgctgcta
      961 cgatgcaccg cgagggattc ttgtgctgca aagtgacaga cacattgaac ggggagaggg
     1021 tctcttttcc cgtgtgcacg tatgtgccag ctacattgtg tgaccaaatg actggcatac
     1081 tggcaacaga tgtcagtgcg gacgacgcgc aaaaactgct ggttgggctc aaccagcgta
     1141 tagtcgtcaa cggtcgcacc cagagaaaca ccaataccat gaaaaattac cttttgcccg
     1201 tagtggccca ggcatttgct aggtgggcaa aggaatataa ggaagatcaa gaagatgaaa
     1261 ggccactagg actacgagat agacagttag tcatggggtg ttgttgggct tttagaaggc
     1321 acaagataac atctatttat aagcgcccgg atacccaaac catcatcaaa gtgaacagcg
     1381 atttccactc attcgtgctg cccaggatag gcagtaacac attggagatc gggctgagaa
     1441 caagaatcag gaaaatgtta gaggagcaca aggagccgtc acctctcatt accgccgagg
     1501 acgtacaaga agctaagtgc gcagccgatg aggctaagga ggtgcgtgaa gccgaggagt
     1561 tgcgcgcagc tctaccacct ttggcagctg atgttgagga gcccactctg gaagccgatg
     1621 tcgacttgat gttacaagag gctggggccg gctcagtgga gacacctcgt ggcttgataa
     1681 aggttaccag ctacgatggc gaggacaaga tcggctctta cgctgtgctt tctccgcagg
     1741 ctgtactcaa gagtgaaaaa ttatcttgca tccaccctct cgctgaacaa gtcatagtga
     1801 taacacactc tggccgaaaa gggcgttatg ccgtggaacc ataccatggt aaagtagtgg
     1861 tgccagaggg acatgcaata cccgtccagg actttcaagc tctgagtgaa agtgccacca
     1921 ttgtgtacaa cgaacgtgag ttcgtaaaca ggtacctgca ccatattgcc acacatggag
     1981 gagcgctgaa cactgatgaa gaatattaca aaactgtcaa gcccagcgag cacgacggcg
     2041 aatacctgta cgacatcgac aggaaacagt gcgtcaagaa agaactagtc actgggctag
     2101 ggctcacagg cgagctggtg gatcctccct tccatgaatt cgcctacgag agtctgagaa
     2161 cacgaccagc cgctccttac caagtaccaa ccataggggt gtatggcgtg ccaggatcag
     2221 gcaagtctgg catcattaaa agcgcagtca ccaaaaaaga tctagtggtg agcgccaaga
     2281 aagaaaactg tgcagaaatt ataagggacg tcaagaaaat gaaagggctg gacgtcaatg
     2341 ccagaactgt ggactcagtg ctcttgaatg gatgcaaaca ccccgtagag accctgtata
     2401 ttgacgaagc ttttgcttgt catgcaggta ctctcagagc gctcatagcc attataagac
     2461 ctaaaaaggc agtgctctgc ggggatccca aacagtgcgg tttttttaac atgatgtgcc
     2521 tgaaagtgca ttttaaccac gagatttgca cacaagtctt ccacaaaagc atctctcgcc
     2581 gttgcactaa atctgtgact tcggtcgtct caaccttgtt ttacgacaaa aaaatgagaa
     2641 cgacgaatcc gaaagagact aagattgtga ttgacactac cggcagtacc aaacctaagc
     2701 aggacgatct cattctcact tgtttcagag ggtgggtgaa gcagttgcaa atagattaca
     2761 aaggcaacga aataatgacg gcagctgcct ctcaagggct gacccgtaaa ggtgtgtatg
     2821 ccgttcggta caaggtgaat gaaaatcctc tgtacgcacc cacctcagaa catgtgaacg
     2881 tcctactgac ccgcacggag gaccgcatcg tgtggaaaac actagccggc gacccatgga
     2941 taaaaacact gactgccaag taccctggga atttcactgc cacgatagag gagtggcaag
     3001 cagagcatga tgccatcatg aggcacatct tggagagacc ggaccctacc gacgtcttcc
     3061 agaataaggc aaacgtgtgt tgggccaagg ctttagtgcc ggtgctgaag accgctggca
     3121 tagacatgac cactgaacaa tggaacactg tggattattt tgaaacggac aaagctcact
     3181 cagcagagat agtattgaac caactatgcg tgaggttctt tggactcgat ctggactccg
     3241 gtctattttc tgcacccact gttccgttat ccattaggaa taatcactgg gataactccc
     3301 cgtcgcctaa catgtacggg ctgaataaag aagtggtccg tcagctctct cgcaggtacc
     3361 cacaactgcc tcgggcagtt gccactggaa gagtctatga catgaacact ggtacactgc
     3421 gcaattatga tccgcgcata aacctagtac ctgtaaacag aagactgcct catgctttag
     3481 tcctccacca taatgaacac ccacagagtg acttttcttc attcgtcagc aaattgaagg
     3541 gcagaactgt cctggtggtc ggggaaaagt tgtccgtccc aggcaaaatg gttgactggt
     3601 tgtcagaccg gcctgaggct accttcagag ctcggctgga tttaggcatc ccaggtgatg
     3661 tgcccaaata tgacataata tttgttaatg tgaggacccc atataaatac catcactatc
     3721 agcagtgtga agaccatgcc attaagctta gcatgttgac caagaaagct tgtctgcatc
     3781 tgaatcccgg cggaacctgt gtcagcatag gttatggtta cgctgacagg gccagcgaaa
     3841 gcatcattgg tgctatagcg cggctgttca agttttcccg ggtatgcaaa ccgaaatcct
     3901 cacttgaaga gacggaagtt ctgtttgtat tcattgggta cgatcgcaag gcccgtacgc
     3961 acaatcctta caagctttca tcaaccttga ccaacattta tacaggttcc agactccacg
     4021 aagccggatg tgcaccctca tatcatgtgg tgcgagggga tattgccacg gccaccgaag
     4081 gagtgattat aaatgctgct aacagcaaag gacaacctgg cggaggggtg tgcggagcgc
     4141 tgtataagaa attcccggaa agcttcgatt tacagccgat cgaagtagga aaagcgcgac
     4201 tggtcaaagg tgcagctaaa catatcattc atgccgtagg accaaacttc aacaaagttt
     4261 cggaggttga aggtgacaaa cagttggcag aggcttatga gtccatcgct aagattgtca
     4321 acgataacaa ttacaagtca gtagcgattc cactgttgtc caccggcatc ttttccggga
     4381 acaaagatcg actaacccaa tcattgaacc atttgctgac agctttagac accactgatg
     4441 cagatgtagc catatactgc agggacaaga aatgggaaat gactctcaag gaagcagtgg
     4501 ctaggagaga agcagtggag gagatatgca tatccgacga ctcttcagtg acagaacctg
     4561 atgcagagct ggtgagggtg catccgaaga gttctttggc tggaaggaag ggctacagca
     4621 caagcgatgg caaaactttc tcatatttgg aagggaccaa gtttcaccag gcggccaagg
     4681 atatagcaga aattaatgcc atgtggcccg ttgcaacgga ggccaatgag caggtatgca
     4741 tgtatatcct cggagaaagc atgagcagta ttaggtcgaa atgccccgtc gaagagtcgg
     4801 aagcctccac accacctagc acgctgcctt gcttgtgcat ccatgccatg actccagaaa
     4861 gagtacagcg cctaaaagcc tcacgtccag aacaaattac tgtgtgctca tcctttccat
     4921 tgccgaagta tagaatcact ggtgtgcaga agatccaatg ctcccagcct atattgttct
     4981 caccgaaagt gcctgcgtat attcatccaa ggaagtatct cgtggaaaca ccaccggtag
     5041 acgagactcc ggagccatcg gcagagaacc aatccacaga ggggacacct gaacaaccac
     5101 cacttataac cgaggatgag accaggacta gaacgcctga gccgatcatc atcgaagagg
     5161 aagaagagga tagcataagt ttgctgtcag atggcccgac ccaccaggtg ctgcaagtcg
     5221 aggcagacat tcacgggccg ccctctgtat ctagctcatc ctggtccatt cctcatgcat
     5281 ccgactttga tgtggacagt ttatccatac ttgacaccct ggagggagct agcgtgacca
     5341 gcggggcaac gtcagccgag actaactctt acttcgcaaa gagtatggag tttctggcgc
     5401 gaccggtgcc tgcgcctcga acagtattca ggaaccctcc acatcccgct ccgcgcacaa
     5461 gaacaccgtc acttgcaccc agcagggcct gctcgagaac cagcctagtt tccaccccgc
     5521 caggcgtgaa tagggtgatc actagagagg agctcgaggc gcttaccccg tcacgcactc
     5581 ctagcaggtc ggtctcgaga accagcctgg tctccaaccc gccaggcgta aatagggtga
     5641 ttacaagaga ggagtttgag gcgttcgtag cacaacaaca atgacggttt gatgcgggtg
     5701 catacatctt ttcctccgac accggtcaag ggcatttaca acaaaaatca gtaaggcaaa
     5761 cggtgctatc cgaagtggtg ttggagagga ccgaattgga gatttcgtat gccccgcgcc
     5821 tcgaccaaga aaaagaagaa ttactacgca agaaattaca gttaaatccc acacctgcta
     5881 acagaagcag ataccagtcc aggaaggtgg agaacatgaa agccataaca gctagacgta
     5941 ttctgcaagg cctagggcat tatttgaagg cagaaggaaa agtggagtgc taccgaaccc
     6001 tgcatcctgt tcctttgtat tcatctagtg tgaaccgtgc cttttcaagc cccaaggtcg
     6061 cagtggaagc ctgtaacgcc atgttgaaag agaactttcc gactgtggct tcttactgta
     6121 ttattccaga gtacgatgcc tatttggaca tggttgacgg agcttcatgc tgcttagaca
     6181 ctgccagttt ttgccctgca aagctgcgca gctttccaaa gaaacactcc tatttggaac
     6241 ccacaatacg atcggcagtg ccttcagcga tccagaacac gctccagaac gtcctggcag
     6301 ctgccacaaa aagaaattgc aatgtcacgc aaatgagaga attgcccgta ttggattcgg
     6361 cggcctttaa tgtggaatgc ttcaagaaat atgcgtgtaa taatgaatat tgggaaacgt
     6421 ttaaagaaaa ccccatcagg cttactgaag aaaacgtggt aaattacatt accaaattaa
     6481 aaggaccaaa agctgctgct ctttttgcga agacacataa tttgaatatg ttgcaggaca
     6541 taccaatgga caggtttgta atggacttaa agagagacgt gaaagtgact ccaggaacaa
     6601 aacatactga agaacggccc aaggtacagg tgatccaggc tgccgatccg ctagcaacag
     6661 cgtatctgtg cggaatccac cgagagctgg ttaggagatt aaatgcggtc ctgcttccga
     6721 acattcatac actgtttgat atgtcggctg aagactttga cgctattata gccgagcact
     6781 tccagcctgg ggattgtgtt ctggaaactg acatcgcgtc gtttgataaa agtgaggacg
     6841 acgccatggc tctgaccgcg ttaatgattc tggaagactt aggtgtggac gcagagctgt
     6901 tgacgctgat tgaggcggct ttcggcgaaa tttcatcaat acatttgccc actaaaacta
     6961 aatttaaatt cggagccatg atgaaatctg gaatgttcct cacactgttt gtgaacacag
     7021 tcattaacat tgtaatcgca agcagagtgt tgagagaacg gctaaccgga tcaccatgtg
     7081 cagcattcat tggagatgac aatatcgtga aaggagtcaa atcggacaaa ttaatggcag
     7141 acaggtgcgc cacctggttg aatatggaag tcaagattat agatgctgtg gtgggcgaga
     7201 aagcgcctta tttctgtgga gggtttattt tgtgtgactc cgtgaccggc acagcgtgcc
     7261 gtgtggcaga ccccctaaaa aggctgttta agcttggcaa acctctggca gcagacgatg
     7321 aacatgatga tgacaggaga agggcattgc atgaagagtc aacacgctgg aaccgagtgg
     7381 gtattctttc agagctgtgc aaggcagtag aatcaaggta tgaaaccgta ggaacttcca
     7441 tcatagttat ggccatgact actctagcta gcagtgttaa atcattcagc tacctgagag
     7501 gggcccctat aactctctac ggctaacctg aatggactac gacatagtct agtccgccaa
     7561 gtctagaaca agtttgtaca aaaaagcagg ctgccaccat ggtgagcaag ggcgaggagc
     7621 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     7681 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     7741 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     7801 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     7861 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     7921 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     7981 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     8041 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     8101 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     8161 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     8221 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     8281 ccgggatcac tctcggcatg gacgagctgt acaagaagct tagccatggc ttcccgccgg
     8341 aggtggagga gcaggatgat ggcacgctgc ccatgtcttg tgcccaggag agcgggatgg
     8401 accgtcaccc tgcagcctgt gcttctgcta ggatcaatgt gtaaacccag ctttcttgta
     8461 caaagtggtg gcgcgcctat gttacgtgca aaggtgattg tcaccccccg aaagaccata
     8521 ttgtgacaca ccctcagtat cacgcccaaa catttacagc cgcggtgtca aaaaccgcgt
     8581 ggacgtggtt aacatccctg ctgggaggat cagccgtaat tattataatt ggcttggtgc
     8641 tggctactat tgtggccatg tacgtgctga ccaaccagaa acataattga atacagcagc
     8701 aattggcaag ctgcttacat agaactcgcg gcgattggca tgccgcctta aaatttttat
     8761 tttatttttc ttttcttttc cgaatcggat tttgttttta atatttcaaa aaaaaaaaaa
     8821 aaaaaaaaaa aaaaaaaaaa aaaaaaatag ggataacagg gtaattgagc aaaaggccag
     8881 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8941 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9001 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9061 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9601 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9661 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9721 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9781 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9841 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9901 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9961 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    10021 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10081 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10141 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10201 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10261 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10321 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10381 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10441 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10501 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10561 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10621 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgttagg
    10681 gataacaggg taattaatac gactcactat ag
//
