LOCUS       pTK331                 12101 bp    DNA     circular UNA 31-JUL-2014
DEFINITION  TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak
            mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre
            attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI.
ACCESSION   urn.local.tasuku.1399168377950.152
VERSION     urn.local.tasuku.1399168377950.152
KEYWORDS    .
SOURCE      VEE
  ORGANISM  VEE
            .
FEATURES             Location/Qualifiers
     5'UTR           1..44
     Mutation        3
                     /label="A->G to create Frolov's """"5' VEE"""" version of
                     TC-83"
     CDS             45..1649
                     /product="nsP1"
                     /note="putative"
                     /label="nsP1"
     misc_feature    106..107
                     /note="this correction results in changing amino acid
                     nsP1-21-Thr to nsP1-21-Ser, this 1992 data corrects the
                     sequence reported in the reference"
                     /citation="[1]; [2]"
                     /replace="gc"
                     /label="Corrected sequence"
     misc_feature    134..184
                     /label="51 nucleotide conserved sequence"
     primer_bind     286..305
                     /label="1F"
     primer_bind     421..443
                     /label="TK-VEE-nsP1-IDT-qPCR-F"
     primer_bind     complement(510..529)
                     /label="1R"
     primer_bind     complement(542..563)
                     /label="TK-VEE-nsP1-IDT-qPCR-R"
     primer_bind     1188..1207
                     /label="2F"
     primer_bind     complement(1391..1410)
                     /label="2R"
     old_sequence    1533..1534
                     /note="this correction changes amino acid nsP1-497-Arg to
                     nsP1-497-Ala, this data corrects the sequence reported in
                     the reference"
                     /citation="[1]; [2]"
                     /replace="gc"
     CDS             1650..4031
                     /product="nsP2"
                     /function="replication"
                     /note="putative"
                     /label="nsP2"
     Mutation        1696
                     /label="TRD/TC-83 discrepency"
     primer_bind     1776..1797
                     /label="TK-VEE-nsP2-IDT-qPCR-R"
     primer_bind     complement(1849..1869)
                     /label="TK-VEE-nsP2-IDT-qPCR-F"
     primer_bind     2055..2074
                     /label="3F"
     primer_bind     complement(2262..2281)
                     /label="3R"
     primer_bind     2874..2893
                     /label="4F"
     primer_bind     complement(3110..3129)
                     /label="4R"
     primer_bind     3771..3790
                     /label="5F"
     Mutation        3864..3866
                     /label="Q739L noncytopathic mutation (CAG->CTG)"
     misc_feature    3865
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(4024..4043)
                     /label="5R"
     CDS             4032..5702
                     /product="nsP3"
                     /function="replication"
                     /note="putative"
                     /label="nsP3"
     primer_bind     4600..4619
                     /label="6F"
     primer_bind     complement(4853..4872)
                     /label="6R"
     primer_bind     5489..5508
                     /label="7F"
     CDS             5703..7523
                     /product="nsP4"
                     /function="replication"
                     /note="putative"
                     /label="nsP4"
     primer_bind     complement(5797..5816)
                     /label="7R"
     primer_bind     6425..6446
                     /label="8F"
     primer_bind     complement(6663..6682)
                     /label="8R"
     primer_bind     7303..7322
                     /label="9F"
     primer_bind     7306..7322
                     /label="TK-VEE-nsP4-mKate-IDT-qPCR-F4"
     gBlock          7306..7705
                     /label="TK-new-VEE-nsP4-SGP(14)-mKate-IDT"
     primer_bind     7339..7358
                     /label="TK-VEE-nsP4-mKate-IDT-qPCR-F3"
     gBlock          7369..7609
                     /label="TK-VEE-nsP4-SGP(14)-mKate-IDT"
     primer_bind     7369..7389
                     /label="Binding Region"
     primer_bind     7426..7447
                     /label="Binding Region"
     primer_bind     7451..7472
                     /label="TK-VEE-nsP4-seq-F"
     primer_bind     7487..7577
                     /label="TK-ApaI-SGP(14)-Kozak-mKate-SapI-F"
     primer_bind     complement(7487..7577)
                     /label="TK-ApaI-SGP(14)-Kozak-mKate-SapI-R"
     gBlock          7499..7932
                     /label="TK-VEE-nsP4-SGP(14)-mKate"
     primer_bind     7499..7518
                     /label="TK-VEE-nsP4-EGFP/mKate-qPCR-F"
     misc_feature    7532..7545
                     /label="Subgenomic RNA 5'UTR SGP(14)"
     misc_RNA        7546..7551
                     /label="Kozak"
     CDS             7552..8262
                     /label="mKate"
     primer_bind     complement(7582..7602)
                     /label="TK-VEE-nsP4-mKate-IDT-qPCR-R1"
     primer_bind     complement(7590..7609)
                     /label="TK-VEE-nsP4-mKate-IDT-qPCR-R2"
     primer_bind     complement(7615..7634)
                     /label="TK-VEE-nsP4-mKate-qPCR-R"
     primer_bind     complement(7618..7638)
                     /label="TK-VEE-nsP4-mKate-IDT-qPCR-R3"
     primer_bind     7625..7644
                     /label="TK-mKate-qPCR-F"
     primer_bind     7674..7694
                     /label="TK-new-mKate-IDT-qPCR-F"
     primer_bind     complement(7687..7705)
                     /label="TK-VEE-nsP4-mKate-IDT-qPCR-R4"
     primer_bind     complement(7724..7743)
                     /label="TK-mKate-qPCR-R"
     primer_bind     complement(7766..7784)
                     /label="TK-new-mKate-IDT-qPCR-R"
     primer_bind     7836..7857
                     /label="TK-mKate-IDT-qPCR-F"
     primer_bind     complement(7911..7932)
                     /label="TK-mKate-IDT-qPCR-R"
     CDS             8263..8286
                     /label="G8"
     CDS             8287..8643
                     /label="L7Ae"
     CDS             8644..8769
                     /label="PEST"
     misc_RNA        8784..8805
                     /label="FF4"
     misc_RNA        8806..8827
                     /label="FF4"
     misc_RNA        8828..8849
                     /label="FF4"
     misc_RNA        8850..8871
                     /label="FF4"
     attB2           complement(8882..8906)
     misc_feature    8882..8884
                     /label="ACC = Fusion site N"
     attB2           complement(8884..8905)
     misc_feature    complement(8892..8898)
                     /label="attB2 x attP2 core recombination site"
     primer_bind     8898..8936
                     /label="TK-attB2-AscI-SGP(-98)-F"
     promoter        8913..9040
                     /label="SGP2(-98/30)"
     CDS             <8913..9002
                     /product="nsP4"
                     /function="replication"
                     /note="putative"
                     /label="nsP4"
     primer_bind     8930..8951
                     /label="TK-VEE-nsP4-seq-F"
     misc_feature    9011..9040
                     /label="Subgenomic RNA 5'UTR"
     misc_feature    9041..9046
                     /label="XbaI site"
     attB1           9047..9071
     misc_RNA        9072..9077
                     /label="Kozak"
     cds             9078..9797
                     /label="EBFP2"
     CDS             9078..9794
                     /label="EBFP2"
     primer_bind     complement(9144..9163)
                     /label="TK-VEE-nsP4-EGFP-IDT-qPCR-R1"
     primer_bind     complement(9350..9369)
                     /label="TK-EYFP-seq-R"
     attB2           complement(9798..9822)
     Mutation        9823..9859
                     /label="Insert (cloning artifact; shouldn't affect
                     construct)"
     misc_feature    9860..9866
                     /label="AscI site"
     CDS             <9867..10076
                     /label="E1 (truncated)"
     3'UTR           10080..10196
     primer_bind     complement(10127..10149)
                     /label="TK-VEE-3'UTR-seq-R"
     misc_feature    10178..10196
                     /label="19 nucleotide conserved sequence"
     misc_RNA        10197..10236
                     /label="Poly A"
     misc_feature    10237..10254
                     /label="I-SceI site"
     primer_bind     complement(10255..10277)
                     /label="TK-PCR126-6-10-R"
     rep_origin      complement(10311..10899)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
     misc_RNA        complement(10311..10863)
                     /note="RNAII transcript (complementary strand)"
     primer_bind     complement(10659..10678)
                     /label="RepO-R"
     -35_signal      10717..10722
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      10739..10744
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        10753..10860
                     /note="RNAI transcript"
     -10_signal      complement(10873..10878)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(10894..10899)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     gene            complement(11070..11930)
                     /gene="bla"
     CDS             complement(11070..11930)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     primer_bind     11693..11712
                     /label="Amp-R"
     sig_peptide     complement(11862..11930)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
     -10_signal      complement(11974..11979)
                     /note="bla promoter (counter-clockwise); GAGACA"
     -35_signal      complement(11995..12000)
                     /note="bla promoter (counter-clockwise); TTCAAA"
     primer_bind     12046..12065
                     /label="TK-PCR126-1-5-F"
     misc_feature    12066..12083
                     /label="I-SceI site"
     promoter        12084..12101
                     /label="T7 promoter"
     misc_feature    12101..44
                     /label="T7 transcript start"
     misc_feature    12101..1
                     /Note="GG would increase txn 2-fold but viral sequence
                     starts with A"
                     /label="Promoter note"
ORIGIN      
        1 atgggcggcg catgagagaa gcccagacca attacctacc caaaatggag aaagttcacg
       61 ttgacatcga ggaagacagc ccattcctca gagctttgca gcggagcttc ccgcagtttg
      121 aggtagaagc caagcaggtc actgataatg accatgctaa tgccagagcg ttttcgcatc
      181 tggcttcaaa actgatcgaa acggaggtgg acccatccga cacgatcctt gacattggaa
      241 gtgcgcccgc ccgcagaatg tattctaagc acaagtatca ttgtatctgt ccgatgagat
      301 gtgcggaaga tccggacaga ttgtataagt atgcaactaa gctgaagaaa aactgtaagg
      361 aaataactga taaggaattg gacaagaaaa tgaaggagct cgccgccgtc atgagcgacc
      421 ctgacctgga aactgagact atgtgcctcc acgacgacga gtcgtgtcgc tacgaagggc
      481 aagtcgctgt ttaccaggat gtatacgcgg ttgacggacc gacaagtctc tatcaccaag
      541 ccaataaggg agttagagtc gcctactgga taggctttga caccacccct tttatgttta
      601 agaacttggc tggagcatat ccatcatact ctaccaactg ggccgacgaa accgtgttaa
      661 cggctcgtaa cataggccta tgcagctctg acgttatgga gcggtcacgt agagggatgt
      721 ccattcttag aaagaagtat ttgaaaccat ccaacaatgt tctattctct gttggctcga
      781 ccatctacca cgagaagagg gacttactga ggagctggca cctgccgtct gtatttcact
      841 tacgtggcaa gcaaaattac acatgtcggt gtgagactat agttagttgc gacgggtacg
      901 tcgttaaaag aatagctatc agtccaggcc tgtatgggaa gccttcaggc tatgctgcta
      961 cgatgcaccg cgagggattc ttgtgctgca aagtgacaga cacattgaac ggggagaggg
     1021 tctcttttcc cgtgtgcacg tatgtgccag ctacattgtg tgaccaaatg actggcatac
     1081 tggcaacaga tgtcagtgcg gacgacgcgc aaaaactgct ggttgggctc aaccagcgta
     1141 tagtcgtcaa cggtcgcacc cagagaaaca ccaataccat gaaaaattac cttttgcccg
     1201 tagtggccca ggcatttgct aggtgggcaa aggaatataa ggaagatcaa gaagatgaaa
     1261 ggccactagg actacgagat agacagttag tcatggggtg ttgttgggct tttagaaggc
     1321 acaagataac atctatttat aagcgcccgg atacccaaac catcatcaaa gtgaacagcg
     1381 atttccactc attcgtgctg cccaggatag gcagtaacac attggagatc gggctgagaa
     1441 caagaatcag gaaaatgtta gaggagcaca aggagccgtc acctctcatt accgccgagg
     1501 acgtacaaga agctaagtgc gcagccgatg aggctaagga ggtgcgtgaa gccgaggagt
     1561 tgcgcgcagc tctaccacct ttggcagctg atgttgagga gcccactctg gaagccgatg
     1621 tcgacttgat gttacaagag gctggggccg gctcagtgga gacacctcgt ggcttgataa
     1681 aggttaccag ctacgatggc gaggacaaga tcggctctta cgctgtgctt tctccgcagg
     1741 ctgtactcaa gagtgaaaaa ttatcttgca tccaccctct cgctgaacaa gtcatagtga
     1801 taacacactc tggccgaaaa gggcgttatg ccgtggaacc ataccatggt aaagtagtgg
     1861 tgccagaggg acatgcaata cccgtccagg actttcaagc tctgagtgaa agtgccacca
     1921 ttgtgtacaa cgaacgtgag ttcgtaaaca ggtacctgca ccatattgcc acacatggag
     1981 gagcgctgaa cactgatgaa gaatattaca aaactgtcaa gcccagcgag cacgacggcg
     2041 aatacctgta cgacatcgac aggaaacagt gcgtcaagaa agaactagtc actgggctag
     2101 ggctcacagg cgagctggtg gatcctccct tccatgaatt cgcctacgag agtctgagaa
     2161 cacgaccagc cgctccttac caagtaccaa ccataggggt gtatggcgtg ccaggatcag
     2221 gcaagtctgg catcattaaa agcgcagtca ccaaaaaaga tctagtggtg agcgccaaga
     2281 aagaaaactg tgcagaaatt ataagggacg tcaagaaaat gaaagggctg gacgtcaatg
     2341 ccagaactgt ggactcagtg ctcttgaatg gatgcaaaca ccccgtagag accctgtata
     2401 ttgacgaagc ttttgcttgt catgcaggta ctctcagagc gctcatagcc attataagac
     2461 ctaaaaaggc agtgctctgc ggggatccca aacagtgcgg tttttttaac atgatgtgcc
     2521 tgaaagtgca ttttaaccac gagatttgca cacaagtctt ccacaaaagc atctctcgcc
     2581 gttgcactaa atctgtgact tcggtcgtct caaccttgtt ttacgacaaa aaaatgagaa
     2641 cgacgaatcc gaaagagact aagattgtga ttgacactac cggcagtacc aaacctaagc
     2701 aggacgatct cattctcact tgtttcagag ggtgggtgaa gcagttgcaa atagattaca
     2761 aaggcaacga aataatgacg gcagctgcct ctcaagggct gacccgtaaa ggtgtgtatg
     2821 ccgttcggta caaggtgaat gaaaatcctc tgtacgcacc cacctcagaa catgtgaacg
     2881 tcctactgac ccgcacggag gaccgcatcg tgtggaaaac actagccggc gacccatgga
     2941 taaaaacact gactgccaag taccctggga atttcactgc cacgatagag gagtggcaag
     3001 cagagcatga tgccatcatg aggcacatct tggagagacc ggaccctacc gacgtcttcc
     3061 agaataaggc aaacgtgtgt tgggccaagg ctttagtgcc ggtgctgaag accgctggca
     3121 tagacatgac cactgaacaa tggaacactg tggattattt tgaaacggac aaagctcact
     3181 cagcagagat agtattgaac caactatgcg tgaggttctt tggactcgat ctggactccg
     3241 gtctattttc tgcacccact gttccgttat ccattaggaa taatcactgg gataactccc
     3301 cgtcgcctaa catgtacggg ctgaataaag aagtggtccg tcagctctct cgcaggtacc
     3361 cacaactgcc tcgggcagtt gccactggaa gagtctatga catgaacact ggtacactgc
     3421 gcaattatga tccgcgcata aacctagtac ctgtaaacag aagactgcct catgctttag
     3481 tcctccacca taatgaacac ccacagagtg acttttcttc attcgtcagc aaattgaagg
     3541 gcagaactgt cctggtggtc ggggaaaagt tgtccgtccc aggcaaaatg gttgactggt
     3601 tgtcagaccg gcctgaggct accttcagag ctcggctgga tttaggcatc ccaggtgatg
     3661 tgcccaaata tgacataata tttgttaatg tgaggacccc atataaatac catcactatc
     3721 agcagtgtga agaccatgcc attaagctta gcatgttgac caagaaagct tgtctgcatc
     3781 tgaatcccgg cggaacctgt gtcagcatag gttatggtta cgctgacagg gccagcgaaa
     3841 gcatcattgg tgctatagcg cggctgttca agttttcccg ggtatgcaaa ccgaaatcct
     3901 cacttgaaga gacggaagtt ctgtttgtat tcattgggta cgatcgcaag gcccgtacgc
     3961 acaatcctta caagctttca tcaaccttga ccaacattta tacaggttcc agactccacg
     4021 aagccggatg tgcaccctca tatcatgtgg tgcgagggga tattgccacg gccaccgaag
     4081 gagtgattat aaatgctgct aacagcaaag gacaacctgg cggaggggtg tgcggagcgc
     4141 tgtataagaa attcccggaa agcttcgatt tacagccgat cgaagtagga aaagcgcgac
     4201 tggtcaaagg tgcagctaaa catatcattc atgccgtagg accaaacttc aacaaagttt
     4261 cggaggttga aggtgacaaa cagttggcag aggcttatga gtccatcgct aagattgtca
     4321 acgataacaa ttacaagtca gtagcgattc cactgttgtc caccggcatc ttttccggga
     4381 acaaagatcg actaacccaa tcattgaacc atttgctgac agctttagac accactgatg
     4441 cagatgtagc catatactgc agggacaaga aatgggaaat gactctcaag gaagcagtgg
     4501 ctaggagaga agcagtggag gagatatgca tatccgacga ctcttcagtg acagaacctg
     4561 atgcagagct ggtgagggtg catccgaaga gttctttggc tggaaggaag ggctacagca
     4621 caagcgatgg caaaactttc tcatatttgg aagggaccaa gtttcaccag gcggccaagg
     4681 atatagcaga aattaatgcc atgtggcccg ttgcaacgga ggccaatgag caggtatgca
     4741 tgtatatcct cggagaaagc atgagcagta ttaggtcgaa atgccccgtc gaagagtcgg
     4801 aagcctccac accacctagc acgctgcctt gcttgtgcat ccatgccatg actccagaaa
     4861 gagtacagcg cctaaaagcc tcacgtccag aacaaattac tgtgtgctca tcctttccat
     4921 tgccgaagta tagaatcact ggtgtgcaga agatccaatg ctcccagcct atattgttct
     4981 caccgaaagt gcctgcgtat attcatccaa ggaagtatct cgtggaaaca ccaccggtag
     5041 acgagactcc ggagccatcg gcagagaacc aatccacaga ggggacacct gaacaaccac
     5101 cacttataac cgaggatgag accaggacta gaacgcctga gccgatcatc atcgaagagg
     5161 aagaagagga tagcataagt ttgctgtcag atggcccgac ccaccaggtg ctgcaagtcg
     5221 aggcagacat tcacgggccg ccctctgtat ctagctcatc ctggtccatt cctcatgcat
     5281 ccgactttga tgtggacagt ttatccatac ttgacaccct ggagggagct agcgtgacca
     5341 gcggggcaac gtcagccgag actaactctt acttcgcaaa gagtatggag tttctggcgc
     5401 gaccggtgcc tgcgcctcga acagtattca ggaaccctcc acatcccgct ccgcgcacaa
     5461 gaacaccgtc acttgcaccc agcagggcct gctcgagaac cagcctagtt tccaccccgc
     5521 caggcgtgaa tagggtgatc actagagagg agctcgaggc gcttaccccg tcacgcactc
     5581 ctagcaggtc ggtctcgaga accagcctgg tctccaaccc gccaggcgta aatagggtga
     5641 ttacaagaga ggagtttgag gcgttcgtag cacaacaaca atgacggttt gatgcgggtg
     5701 catacatctt ttcctccgac accggtcaag ggcatttaca acaaaaatca gtaaggcaaa
     5761 cggtgctatc cgaagtggtg ttggagagga ccgaattgga gatttcgtat gccccgcgcc
     5821 tcgaccaaga aaaagaagaa ttactacgca agaaattaca gttaaatccc acacctgcta
     5881 acagaagcag ataccagtcc aggaaggtgg agaacatgaa agccataaca gctagacgta
     5941 ttctgcaagg cctagggcat tatttgaagg cagaaggaaa agtggagtgc taccgaaccc
     6001 tgcatcctgt tcctttgtat tcatctagtg tgaaccgtgc cttttcaagc cccaaggtcg
     6061 cagtggaagc ctgtaacgcc atgttgaaag agaactttcc gactgtggct tcttactgta
     6121 ttattccaga gtacgatgcc tatttggaca tggttgacgg agcttcatgc tgcttagaca
     6181 ctgccagttt ttgccctgca aagctgcgca gctttccaaa gaaacactcc tatttggaac
     6241 ccacaatacg atcggcagtg ccttcagcga tccagaacac gctccagaac gtcctggcag
     6301 ctgccacaaa aagaaattgc aatgtcacgc aaatgagaga attgcccgta ttggattcgg
     6361 cggcctttaa tgtggaatgc ttcaagaaat atgcgtgtaa taatgaatat tgggaaacgt
     6421 ttaaagaaaa ccccatcagg cttactgaag aaaacgtggt aaattacatt accaaattaa
     6481 aaggaccaaa agctgctgct ctttttgcga agacacataa tttgaatatg ttgcaggaca
     6541 taccaatgga caggtttgta atggacttaa agagagacgt gaaagtgact ccaggaacaa
     6601 aacatactga agaacggccc aaggtacagg tgatccaggc tgccgatccg ctagcaacag
     6661 cgtatctgtg cggaatccac cgagagctgg ttaggagatt aaatgcggtc ctgcttccga
     6721 acattcatac actgtttgat atgtcggctg aagactttga cgctattata gccgagcact
     6781 tccagcctgg ggattgtgtt ctggaaactg acatcgcgtc gtttgataaa agtgaggacg
     6841 acgccatggc tctgaccgcg ttaatgattc tggaagactt aggtgtggac gcagagctgt
     6901 tgacgctgat tgaggcggct ttcggcgaaa tttcatcaat acatttgccc actaaaacta
     6961 aatttaaatt cggagccatg atgaaatctg gaatgttcct cacactgttt gtgaacacag
     7021 tcattaacat tgtaatcgca agcagagtgt tgagagaacg gctaaccgga tcaccatgtg
     7081 cagcattcat tggagatgac aatatcgtga aaggagtcaa atcggacaaa ttaatggcag
     7141 acaggtgcgc cacctggttg aatatggaag tcaagattat agatgctgtg gtgggcgaga
     7201 aagcgcctta tttctgtgga gggtttattt tgtgtgactc cgtgaccggc acagcgtgcc
     7261 gtgtggcaga ccccctaaaa aggctgttta agcttggcaa acctctggca gcagacgatg
     7321 aacatgatga tgacaggaga agggcattgc atgaagagtc aacacgctgg aaccgagtgg
     7381 gtattctttc agagctgtgc aaggcagtag aatcaaggta tgaaaccgta ggaacttcca
     7441 tcatagttat ggccatgact actctagcta gcagtgttaa atcattcagc tacctgagag
     7501 gggcccctat aactctctac ggctaacctg aatggactac gacatgccac catggtgtct
     7561 aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     7621 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     7681 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     7741 accagcttca tgtacggcag caaaaccttc atcaaccaca cccagggcat ccccgacttc
     7801 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     7861 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     7921 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
     7981 tgggaggcct ccaccgagat gctgtacccc gctgacggcg gcctggaagg cagaagcgac
     8041 atggccctga agctcgtggg cgggggccac ctgatctgca acttgaagac cacatacaga
     8101 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga cagaagactg
     8161 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc
     8221 agatactgcg acctccctag caaactgggg cacaaactta atggaggcgg aggcggaggc
     8281 ggaggcatgt acgtgagatt tgaggttcct gaggacatgc agaacgaagc tctgagtctg
     8341 ctggagaagg ttagggagag cggtaaggta aagaaaggta ccaacgagac gacaaaggct
     8401 gtggagaggg gactggcaaa gctcgtttac atcgcagagg atgttgaccc gcctgagatc
     8461 gttgctcatc tgcccctcct ctgcgaggag aagaatgtgc cgtacattta cgttaaaagc
     8521 aagaacgacc ttggaagggc tgtgggcatt gaggtgccat gcgcttcggc agcgataatc
     8581 aacgagggag agctgagaaa ggagcttgga agccttgtgg agaagattaa aggccttcag
     8641 aagaagctta gccatggctt cccgccggag gtggaggagc aggatgatgg cacgctgccc
     8701 atgtcttgtg cccaggagag cgggatggac cgtcaccctg cagcctgtgc ttctgctagg
     8761 atcaatgtgt aactcgacgc gccccgcttg aagtctttaa ttaaaccgct tgaagtcttt
     8821 aattaaaccg cttgaagtct ttaattaaac cgcttgaagt ctttaattaa agctagttac
     8881 cacccagctt tcttgtacaa agtggtggcg cgacttccat catagttatg gccatgacta
     8941 ctctagctag cagtgttaaa tcattcagct acctgagagg ggcccctata actctctacg
     9001 gctaacctga atggactacg acatagtcta gtccgccaag tctagaacaa gtttgtacaa
     9061 aaaagcaggc tgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     9121 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgagg ggcgagggcg
     9181 agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     9241 ccgtgccctg gcccaccctc gtgaccaccc tgagccacgg cgtgcagtgc ttcgcccgct
     9301 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     9361 aggagcgcac catcttcttc aaggacgacg gcacctacaa gacccgcgcc gaggtgaagt
     9421 tcgagggcga caccctagtg aaccgcatcg agctgaaggg cgtcgacttc aaggaggacg
     9481 gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaacatc tatatcatgg
     9541 ccgtcaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac gtggaggacg
     9601 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     9661 tgctgcccga cagccactac ctgagcaccc agtccgtgct gagcaaagac cccaacgaga
     9721 agcgcgatca catggtcctg ctggagttcc gcaccgccgc cgggatcact ctcggcatgg
     9781 acgagctgta caagtaaacc cagctttctt gtacaaagtg gtggcgcgac ttccatcata
     9841 gttatggccc aaagtggtgg cgcgcctatg ttacgtgcaa aggtgattgt caccccccga
     9901 aagaccatat tgtgacacac cctcagtatc acgcccaaac atttacagcc gcggtgtcaa
     9961 aaaccgcgtg gacgtggtta acatccctgc tgggaggatc agccgtaatt attataattg
    10021 gcttggtgct ggctactatt gtggccatgt acgtgctgac caaccagaaa cataattgaa
    10081 tacagcagca attggcaagc tgcttacata gaactcgcgg cgattggcat gccgccttaa
    10141 aatttttatt ttatttttct tttcttttcc gaatcggatt ttgtttttaa tatttcaaaa
    10201 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaatagg gataacaggg taattgagca
    10261 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    10321 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    10381 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    10441 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10501 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10561 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10621 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10681 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10741 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10801 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10861 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10921 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10981 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    11041 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    11101 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    11161 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    11221 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    11281 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    11341 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    11401 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    11461 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    11521 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    11581 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    11641 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    11701 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    11761 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    11821 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    11881 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11941 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    12001 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    12061 gacgttaggg ataacagggt aattaatacg actcactata g
//
