LOCUS       pBSWt1-Dest                  5261 bp    DNA     circular SYN 05-JUN-2014
DEFINITION  pBSWt1-Dest
FEATURES             Location/Qualifiers
     gene            complement(4276..5133)
                     /note="Amp"
     misc_feature    965..967
                     /note="CTG"
                     /note=" start codon of Wt1"
     misc_recomb     683..1168
                     /note="5' homology arm"
     misc_signal     complement(1203..1327)
                     /note="attR1"
     CDS             1421..2215
                     /note="Kan r"
     promoter        1342..1420
                     /note="em7"
     misc_signal     2261..2385
                     /note="attR2"
     misc_feature    2528..3056
                     /note="3' homology arm"
     polyA_signal    2429..2463
                     /note="SV40 early polyA"
ORIGIN      
          1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
         61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
        121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
        181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
        241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
        301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
        361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
        421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
        481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
        541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
        601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
        661 ccgcggtggc ggccgctCTA GCtaggctag ggctgcTGTG TGAATGGAGC GGCCGAGCAT
        721 CCTGGCTCCT CCTCCTTCCC TGCTGCCGGC CCCTCTTATT TGAGCTTTGG GAAGCTGGGG
        781 GCAGCCAGGC AGCTGGGGTA AGGAGTTCAA GGCAGCGCCC ACACCCGGGG CTCTCCGCAA
        841 CCCGACCGCC TGCCTGCTCC CCCTTTCCTT TTCCCGCCCT CCCTCCCACC CACTCATTCA
        901 CCCACCCACC CAGAGAGAGG ACGGCAGCCC AGGAACCCGG GCCCGCCGCC TCCTCGCCGC
        961 GATCCTGGAC TTCCTCCTGT CGCAGGAGCC GGCTTCCACG TGTGTCCCGG AGCCGGCGTC
       1021 TCAGCACACG CTCCGCCGGG AGCCCGGGTG CGTCCAGCAG CCGGAGCAAC CTGGGGACCG
       1081 AGGCCCCCGG AGCGCCTGGG CCAAGTCCAG CGCCGAGAAT CCGCAGGATC GCAGGAGCGG
       1141 AGAACCGTCC GCATCCGAGC CGCACCTCAg atcccccggg ctgcaGACAG CAAGCGGCCA
       1201 TCACAAGTtt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
       1261 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagT
       1321 CACTATGGCG GCCGCGAATT CGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA
       1381 TAATACGACA AGGTGAGGAA CTAAACCCAG GAGGCAGATC ATGATTGAAC AAGATGGATT
       1441 GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA
       1501 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT
       1561 TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAAGACGAGG CAGCGCGGCT
       1621 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC
       1681 GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT
       1741 TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA
       1801 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG
       1861 GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC
       1921 AGCCGAACTG TTCGCCAGGC TCAAGGCGAG CATGCCCGAC GGCGAGGATC TCGTCGTGAC
       1981 CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT
       2041 CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA
       2101 TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC
       2161 CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG
       2221 ACTCTGGGGT TCGAAATGAC CGACAAGCTT GCAGGTCGAC CATAGTGAct ggatatgttg
       2281 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
       2341 tatatcattt tacgtttctc gttcagcttt cttgtacAAA GTGGTGATGG CCGCTGACTC
       2401 GAGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT
       2461 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA
       2521 TTAATTAACG CCGCTTCTCC CCTTGTCTGT TCCTTCCTGA TGCCAGTTCT TACTCAGCAG
       2581 CCTTCCAAGG GAGTCTGAAA GTAGCGGACA GGGAGGAGTG GGCTCTGTCA AATGCGAGTG
       2641 ACAACGCGAC AACGCACCAC CACCACCACC ACTTACACAT TACTGAGCGC AACCCCAGAG
       2701 TCCGATAGTT CGTAGGACGC GGGAGGACAT CTGGGGCCCC GGGGTGTCCT AGAGATTTCG
       2761 GAACTCCACG GGAGGAGGAA GAGGAGGTTG GCTGTAACGT GTGACTCTAC ACCGGGGGAA
       2821 AGTCTGATGC ATAAGGCCTC ACCAGGCGTT GACTCTTAAA ACGTCTAGGG TAGACCAAGA
       2881 TGAGGACTCC GAGTGGTGCT GTGGTTTAGG GACATCCCCC ACCCCCCGCC CCCGTGCTGC
       2941 CTCTGACAAG CCCGAAGAGC CGACCCGAAG AACTTCCAGC TCTCGGTGGG CCCTGGCTCA
       3001 TTCCAGGCCC ACCAGAAGCA GTAGGGAGCT TTGGAATGAG GGATTAACAC TTTGGGTACc
       3061 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
       3121 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
       3181 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
       3241 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
       3301 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
       3361 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
       3421 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
       3481 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
       3541 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
       3601 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
       3661 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
       3721 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
       3781 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
       3841 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
       3901 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
       3961 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
       4021 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
       4081 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
       4141 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
       4201 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
       4261 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
       4321 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
       4381 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
       4441 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
       4501 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
       4561 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
       4621 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
       4681 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
       4741 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
       4801 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
       4861 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
       4921 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
       4981 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
       5041 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
       5101 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
       5161 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
       5221 acaaataggg gttccgcgca catttccccg aaaagtgcca c
//

