LOCUS       pcDNA5/FRT/TO-H2        8859 bp    DNA     circular UNA 29-APR-2013
DEFINITION  Final! (Karginov annotated).
ACCESSION   urn.local...1351805122726.271
VERSION     urn.local...1351805122726.271
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     3'UTR           3117..4785
                     /label="Vamp3delta3"
     conflict        3555..3590
                     /label="Seed Site Removed"
     intron          1973..2805
                     /label="intron"
     ncRNA           2395..2414
     promoter        complement(8724..8822)
                     /label="beta-lac promoter"
     misc_feature    232..958
                     /note="Geneious type: promoter uukaryotic"
                     /label="CMV promoter"
     rep_origin      complement(7045..7718)
                     /label="pUC origin"
     sig_peptide     992..1369
                     /label="H2B"
     ncRNA           2334..2442
     misc_feature    820..859
                     /note="Geneious type: motif"
                     /label="2x Tet Operator"
     polyA_signal    4817..5041
                     /label="BGH polyA"
     polyA_signal    6532..6662
                     /label="SV40 early oplyA"
     misc_feature    3259..3265
                     /note="Geneious type: ncRNA seed site"
                     /label="mir124 Seed Site (7-mer)"
     misc_feature    3324..3330
                     /note="Geneious type: ncRNA seed site"
                     /label="mir124 Seed Site (7-mer)"
     misc_feature    3246..3251
                     /note="Geneious type: ncRNA seed site"
                     /label="mir124 Seed Site (6-mer)"
     gene            5380..6400
                     /label="hygroR (no ATG)"
     gene            complement(7863..8723)
                     /label="ampR"
     gene            1394..1972
                     /label="mCherry Exon 1"
     gene            2806..2937
                     /label="mCherry Exon 2"
     misc_feature    2974..3096
                     /label="PEST clontech"
     misc_feature    5325..5372
                     /label="FRT site"
     misc_feature    1386..1393
                     /label="Kozak"
     misc_feature    984..991
                     /label="Kozak"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag ctttcgccac catgccagag ccagcgaagt ctgctcccgc
     1021 cccgaaaaag ggctccaaga aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg
     1081 caagcgcagc cgcaaggaga gctattccat ctatgtgtac aaggttctga agcaggtcca
     1141 ccctgacacc ggcatttcgt ccaaggccat gggcatcatg aattcgtttg tgaacgacat
     1201 tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat tacaacaagc gctcgaccat
     1261 cacctccagg gagatccaga cggccgtgcg cctgctgctg cctggggagt tggccaagca
     1321 cgccgtgtcc gagggtacta aggccatcac caagtacacc agcgctaagg atccccgggt
     1381 accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     1441 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     1501 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1561 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     1621 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     1681 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     1741 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     1801 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     1861 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     1921 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc aggtgagtac
     1981 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
     2041 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
     2101 gaaaagacaa tctttttgtt gtcaagctgt ttagactcat gcacccgcta ctcttcaacc
     2161 ttgcagacta attaatggca actctgacag atgtggcggg aggaaagctg aggtgggagt
     2221 actgctcaga gctacaactc taggagtagg gactccaagc ctagagctcc aagagagggt
     2281 gaagggcagg gagaaaatta tagtaatagt tgcaatgagt cacttgcttc tagatcaaga
     2341 tcagagactc tgctctccgt gttcacagcg gaccttgatt taatgtcata caattaaggc
     2401 acgcggtgaa tgccaagagc ggagcctacg gctgcacttg aaggacatcc gagagaagtt
     2461 aggaagggtg gggagaaaca attctagaat gaacccatcc tgtgcgacac aaggacccct
     2521 cgcattgatt caaaatcttt agaatacaag acagtcaaat gtttttcagc gagaatctgg
     2581 gaaatccgca tgcatactcc caaggggaat attagaggtt tcattgacag ctgtcaggag
     2641 agctgtagtt cccattggtg caaacggtct aagagatggc actcagagag tgctggcctt
     2701 ttccaaatac tagtcatatg cggccgcagc ttgaggtgtg acaggcttga gatctggcca
     2761 tacacttgag tgacaatgac atccactttg cctttctctc cacagctgcc cggcgcctac
     2821 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     2881 tacgaacgcg ccgagggccg ccactccacc ggtggcatgg acgagctgta caagtctgac
     2941 cttgatatcc agcacagtgg cggccgctcg agtagccatg gcttcccgcc ggcggtggcg
     3001 gcgcaggatg atggcacgct gcccatgtct tgtgcccagg agagcgggat ggaccgtcac
     3061 cctgcagcct gtgcttctgc taggatcaat gtgtagtaat gagtttaagg cgcgcccacc
     3121 gatgaaactg aagcccgata ttcaggatct tcttcaagac tttctactta gaacctgccg
     3181 tgttatcgag cttacctatt gttttttttc caaatgatta tttttgttag tttacataca
     3241 gtttagtgcc tagaaaatgt gcctttgttt ttcatataca ctccaaactg gaggtgtgac
     3301 ctaccctccc cacattttgc acagtgcctt tacagatttc tggactggga aagaggactt
     3361 gtgtagttct gagtgctgaa tggaggtgga tgttgtcact caagatgact tgttgtggat
     3421 tccaggaagg gggttttgtt caaggcagct gtccccacat ctgacatcat cttcaaaacc
     3481 acattttaaa cttttgggta attagattcc taacttggac cttccaagaa gaacactact
     3541 gcctaacagc aatgggtgac aatagcaggg tattttgata gctaggaaac tgttctttaa
     3601 gattattctc cgtttgtaag cactactgac tcccgctgta tttaagatgc tggtgaagag
     3661 ctcatgctct tatgttagat atgactatgt ttactttctt gttcttgttg tatatcactc
     3721 ctaaaatagt attttaacca gaaataaccc tttaagaagt ttttatagaa ctatgaccaa
     3781 agtttctatt ttgccaaaag ttaagtacaa ataaattgcc ccaatagata cattcaaata
     3841 agtgccaaat gtaactcaag gtgtccttta agaaaaatgt taatactgtg agagcgagcc
     3901 ttcaacatgt cggcagtcca tcctgtgaaa tgctatagag aaggctagtc attctgattt
     3961 ggtttcttct ttagagctat ggaggtgggc tcagatcaca cctgtagaca agatacttag
     4021 ccagcagttt gtagactgag ctaggctgtg gtctctagtg cgatgtgagc agtagccgtg
     4081 cagcctcacc aaggcatcag tctgcacaga gttccagaag actgtgtact gcttgtactc
     4141 atctctgtct tatgcttgag gtaagccacc ggtcactcgt agaggatagc taaccagaga
     4201 tgcctggtgc tcttagaatg tagccattgc tgtctgggac agtttcattg tttatatgag
     4261 actttcatca aggcttggtt caaagacagg agttcaggca tgggtggaat tgaggaagtg
     4321 gtgaagggaa agctgcttgc ctatggcagt gccactagaa tgtcctgaaa tctgtgtttg
     4381 tgtgtaaagc agcattactc aagatgtcat agaatctggt tctagttttt tgtttttgtt
     4441 ttgttttttg agacagggtt tctctgtata gcccttgctg tcctggaact cactttgtag
     4501 accaggctgg ctcgaactca aaaatccgcc tgcctctgcc tcccaagtgc agggattaaa
     4561 ggcgtgtgcc accaacgccc aactggttct agttttttat ctgttcccct aacattactg
     4621 aaacaataca gatgttgaag atatgccttg ccgcatcagc ctagggcttc tgtcctggtc
     4681 tgacctcaca actttggtgc tgtctcctca tattctgtcc catgtaactc agtggctcct
     4741 gtattaacac agtgatacct cgcttctctg tgcatttagc tgggagggcc cgtttaaacc
     4801 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     4861 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     4921 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     4981 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     5041 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     5101 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     5161 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5221 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5281 ctcgacccca aaaaacttga ttagggtgat ggttcacgta cctagaagtt cctattccga
     5341 agttcctatt ctctagaaag tataggaact tccttggcca aaaagcctga actcaccgcg
     5401 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     5461 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     5521 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     5581 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     5641 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     5701 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     5761 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     5821 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     5881 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     5941 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     6001 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     6061 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     6121 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     6181 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     6241 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     6301 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     6361 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agcacgtact acgagatttc
     6421 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6481 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     6541 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6601 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6661 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6721 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6781 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6841 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6901 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6961 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7021 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7081 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7141 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7201 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7261 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7321 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7381 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7441 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7501 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     7561 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7621 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7681 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7741 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7801 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7861 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7921 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7981 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8041 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8101 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8161 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8221 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8281 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8341 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8401 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8461 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8521 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8581 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8641 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8701 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8761 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8821 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
