LOCUS       pcDNA5/FRT/TO-H2        8852 bp    DNA     circular UNA 29-APR-2013
DEFINITION  Final! (Karginov annotated).
ACCESSION   urn.local...1351805122613.270
VERSION     urn.local...1351805122613.270
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     3'UTR           3117..4778
                     /label="Vamp3delta23"
     intron          1973..2805
                     /label="intron"
     ncRNA           2395..2414
     primer_bind     1676..1695
                     /label="TS-mCH-qPCR-F.1"
     misc_feature    complement(1798..1817)
                     /note="Geneious type: primer_bind_reverse"
                     /label="TS-mCH-qPCR-R.1"
     promoter        complement(8717..8815)
                     /label="beta-lac promoter"
     misc_feature    232..958
                     /note="Geneious type: promoter uukaryotic"
                     /label="CMV promoter"
     rep_origin      complement(7038..7711)
                     /label="pUC origin"
     sig_peptide     992..1369
                     /label="H2B"
     conflict        3548..3583
                     /label="Seed Site Removed"
     conflict        3306..3340
                     /label="Seed Site Removed"
     ncRNA           2334..2442
     misc_feature    820..859
                     /note="Geneious type: motif"
                     /label="2x Tet Operator"
     misc_feature    3259..3265
                     /note="Geneious type: ncRNA seed site"
                     /label="mir124 Seed Site (7-mer)"
     misc_feature    3246..3251
                     /note="Geneious type: ncRNA seed site"
                     /label="mir124 Seed Site (6-mer)"
     polyA_signal    4810..5034
                     /label="BGH polyA"
     polyA_signal    6525..6655
                     /label="SV40 early oplyA"
     gene            5373..6393
                     /label="hygroR (no ATG)"
     gene            complement(7856..8716)
                     /label="ampR"
     gene            1394..1972
                     /label="mCherry Exon 1"
     gene            2806..2937
                     /label="mCherry Exon 2"
     misc_feature    2974..3096
                     /label="PEST clontech"
     misc_feature    5318..5365
                     /label="FRT site"
     misc_feature    1386..1393
                     /label="Kozak"
     misc_feature    984..991
                     /label="Kozak"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag ctttcgccac catgccagag ccagcgaagt ctgctcccgc
     1021 cccgaaaaag ggctccaaga aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg
     1081 caagcgcagc cgcaaggaga gctattccat ctatgtgtac aaggttctga agcaggtcca
     1141 ccctgacacc ggcatttcgt ccaaggccat gggcatcatg aattcgtttg tgaacgacat
     1201 tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat tacaacaagc gctcgaccat
     1261 cacctccagg gagatccaga cggccgtgcg cctgctgctg cctggggagt tggccaagca
     1321 cgccgtgtcc gagggtacta aggccatcac caagtacacc agcgctaagg atccccgggt
     1381 accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     1441 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     1501 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1561 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     1621 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     1681 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     1741 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     1801 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     1861 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     1921 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc aggtgagtac
     1981 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
     2041 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
     2101 gaaaagacaa tctttttgtt gtcaagctgt ttagactcat gcacccgcta ctcttcaacc
     2161 ttgcagacta attaatggca actctgacag atgtggcggg aggaaagctg aggtgggagt
     2221 actgctcaga gctacaactc taggagtagg gactccaagc ctagagctcc aagagagggt
     2281 gaagggcagg gagaaaatta tagtaatagt tgcaatgagt cacttgcttc tagatcaaga
     2341 tcagagactc tgctctccgt gttcacagcg gaccttgatt taatgtcata caattaaggc
     2401 acgcggtgaa tgccaagagc ggagcctacg gctgcacttg aaggacatcc gagagaagtt
     2461 aggaagggtg gggagaaaca attctagaat gaacccatcc tgtgcgacac aaggacccct
     2521 cgcattgatt caaaatcttt agaatacaag acagtcaaat gtttttcagc gagaatctgg
     2581 gaaatccgca tgcatactcc caaggggaat attagaggtt tcattgacag ctgtcaggag
     2641 agctgtagtt cccattggtg caaacggtct aagagatggc actcagagag tgctggcctt
     2701 ttccaaatac tagtcatatg cggccgcagc ttgaggtgtg acaggcttga gatctggcca
     2761 tacacttgag tgacaatgac atccactttg cctttctctc cacagctgcc cggcgcctac
     2821 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     2881 tacgaacgcg ccgagggccg ccactccacc ggtggcatgg acgagctgta caagtctgac
     2941 cttgatatcc agcacagtgg cggccgctcg agtagccatg gcttcccgcc ggcggtggcg
     3001 gcgcaggatg atggcacgct gcccatgtct tgtgcccagg agagcgggat ggaccgtcac
     3061 cctgcagcct gtgcttctgc taggatcaat gtgtagtaat gagtttaagg cgcgcccacc
     3121 gatgaaactg aagcccgata ttcaggatct tcttcaagac tttctactta gaacctgccg
     3181 tgttatcgag cttacctatt gttttttttc caaatgatta tttttgttag tttacataca
     3241 gtttagtgcc tagaaaatgt gcctttgttt ttcatataca ctccaaactg gaggtgtgac
     3301 ctaccctccc cacattttgc acatacagat ttctggactg ggaaagagga cttgtgtagt
     3361 tctgagtgct gaatggaggt ggatgttgtc actcaagatg acttgttgtg gattccagga
     3421 agggggtttt gttcaaggca gctgtcccca catctgacat catcttcaaa accacatttt
     3481 aaacttttgg gtaattagat tcctaacttg gaccttccaa gaagaacact actgcctaac
     3541 agcaatgggt gacaatagca gggtattttg atagctagga aactgttctt taagattatt
     3601 ctccgtttgt aagcactact gactcccgct gtatttaaga tgctggtgaa gagctcatgc
     3661 tcttatgtta gatatgacta tgtttacttt cttgttcttg ttgtatatca ctcctaaaat
     3721 agtattttaa ccagaaataa ccctttaaga agtttttata gaactatgac caaagtttct
     3781 attttgccaa aagttaagta caaataaatt gccccaatag atacattcaa ataagtgcca
     3841 aatgtaactc aaggtgtcct ttaagaaaaa tgttaatact gtgagagcga gccttcaaca
     3901 tgtcggcagt ccatcctgtg aaatgctata gagaaggcta gtcattctga tttggtttct
     3961 tctttagagc tatggaggtg ggctcagatc acacctgtag acaagatact tagccagcag
     4021 tttgtagact gagctaggct gtggtctcta gtgcgatgtg agcagtagcc gtgcagcctc
     4081 accaaggcat cagtctgcac agagttccag aagactgtgt actgcttgta ctcatctctg
     4141 tcttatgctt gaggtaagcc accggtcact cgtagaggat agctaaccag agatgcctgg
     4201 tgctcttaga atgtagccat tgctgtctgg gacagtttca ttgtttatat gagactttca
     4261 tcaaggcttg gttcaaagac aggagttcag gcatgggtgg aattgaggaa gtggtgaagg
     4321 gaaagctgct tgcctatggc agtgccacta gaatgtcctg aaatctgtgt ttgtgtgtaa
     4381 agcagcatta ctcaagatgt catagaatct ggttctagtt ttttgttttt gttttgtttt
     4441 ttgagacagg gtttctctgt atagcccttg ctgtcctgga actcactttg tagaccaggc
     4501 tggctcgaac tcaaaaatcc gcctgcctct gcctcccaag tgcagggatt aaaggcgtgt
     4561 gccaccaacg cccaactggt tctagttttt tatctgttcc cctaacatta ctgaaacaat
     4621 acagatgttg aagatatgcc ttgccgcatc agcctagggc ttctgtcctg gtctgacctc
     4681 acaactttgg tgctgtctcc tcatattctg tcccatgtaa ctcagtggct cctgtattaa
     4741 cacagtgata cctcgcttct ctgtgcattt agctgggagg gcccgtttaa acccgctgat
     4801 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     4861 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     4921 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     4981 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     5041 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     5101 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5161 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5221 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5281 ccaaaaaact tgattagggt gatggttcac gtacctagaa gttcctattc cgaagttcct
     5341 attctctaga aagtatagga acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg
     5401 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     5461 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     5521 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     5581 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     5641 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     5701 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     5761 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     5821 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     5881 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     5941 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     6001 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     6061 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     6121 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     6181 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     6241 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     6301 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     6361 gacgccccag cactcgtccg agggcaaagg aatagcacgt actacgagat ttcgattcca
     6421 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     6481 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     6541 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6601 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     6661 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6721 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6781 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6841 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6901 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6961 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7021 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7081 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7141 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7201 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7261 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7321 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7381 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7441 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7501 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7561 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7621 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7681 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7741 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7801 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7861 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7921 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7981 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8041 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8101 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8161 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8221 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8281 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8341 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8401 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8461 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8521 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8581 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8641 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8701 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8761 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8821 gcacatttcc ccgaaaagtg ccacctgacg tc
//
