LOCUS       pcDNA5/FRT/TO-H2        7198 bp    DNA     circular UNA 29-APR-2013
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...1364514949874.25
VERSION     urn.local...1364514949874.25
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     intron          1973..2805
                     /label="intron"
     promoter        complement(7063..7161)
                     /label="beta-lac promoter"
     misc_feature    232..958
                     /note="Geneious type: promoter uukaryotic"
                     /label="CMV promoter"
     rep_origin      complement(5384..6057)
                     /label="pUC origin"
     polyA_signal    3156..3380
                     /label="BGH polyA"
     polyA_signal    4871..5001
                     /label="SV40 early oplyA"
     sig_peptide     992..1369
                     /label="H2B"
     sig_peptide     2974..3096
                     /label="Pest Sequence"
     misc_feature    2334..2442
                     /note="Geneious type: motif"
                     /label="mir124 stem-loop"
     misc_feature    820..859
                     /note="Geneious type: motif"
                     /label="2x Tet Operator"
     ncRNA           2395..2414
     gene            3719..4739
                     /label="hygroR (no ATG)"
     gene            complement(6202..7062)
                     /label="ampR"
     gene            1394..1972
                     /label="mCherry Exon 1"
     gene            2806..2937
                     /label="mCherry Exon 2"
     misc_feature    2133..2708
                     /label="mouse mir-124-3 gene"
     misc_feature    3664..3711
                     /label="FRT site"
     misc_feature    1386..1393
                     /label="Kozak"
     misc_feature    984..991
                     /label="Kozak"
     misc_feature    1369..1374
                     /note="Geneious type: restriction site"
                     /label="BamHI"
     misc_feature    1378..1383
                     /note="Geneious type: restriction site"
                     /label="KpnI"
     misc_feature    6495..6500
                     /note="Geneious type: restriction site"
                     /label="AviII"
     misc_feature    3125..3130
                     /note="Geneious type: restriction site"
                     /label="PspOMI"
     misc_feature    228..233
                     /note="Geneious type: restriction site"
                     /label="MluI"
     misc_feature    161..166
                     /note="Geneious type: restriction site"
                     /label="MfeI"
     misc_feature    2967..2972
                     /note="Geneious type: restriction site"
                     /label="XhoI"
     misc_feature    2590..2595
                     /note="Geneious type: restriction site"
                     /label="NsiI"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag ctttcgccac catgccagag ccagcgaagt ctgctcccgc
     1021 cccgaaaaag ggctccaaga aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg
     1081 caagcgcagc cgcaaggaga gctattccat ctatgtgtac aaggttctga agcaggtcca
     1141 ccctgacacc ggcatttcgt ccaaggccat gggcatcatg aattcgtttg tgaacgacat
     1201 tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat tacaacaagc gctcgaccat
     1261 cacctccagg gagatccaga cggccgtgcg cctgctgctg cctggggagt tggccaagca
     1321 cgccgtgtcc gagggtacta aggccatcac caagtacacc agcgctaagg atccccgggt
     1381 accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     1441 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     1501 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1561 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     1621 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     1681 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     1741 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     1801 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     1861 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     1921 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc aggtgagtac
     1981 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
     2041 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
     2101 gaaaagacaa tctttttgtt gtcaagctgt ttagactcat gcacccgcta ctcttcaacc
     2161 ttgcagacta attaatggca actctgacag atgtggcggg aggaaagctg aggtgggagt
     2221 actgctcaga gctacaactc taggagtagg gactccaagc ctagagctcc aagagagggt
     2281 gaagggcagg gagaaaatta tagtaatagt tgcaatgagt cacttgcttc tagatcaaga
     2341 tcagagactc tgctctccgt gttcacagcg gaccttgatt taatgtcata caattaaggc
     2401 acgcggtgaa tgccaagagc ggagcctacg gctgcacttg aaggacatcc gagagaagtt
     2461 aggaagggtg gggagaaaca attctagaat gaacccatcc tgtgcgacac aaggacccct
     2521 cgcattgatt caaaatcttt agaatacaag acagtcaaat gtttttcagc gagaatctgg
     2581 gaaatccgca tgcatactcc caaggggaat attagaggtt tcattgacag ctgtcaggag
     2641 agctgtagtt cccattggtg caaacggtct aagagatggc actcagagag tgctggcctt
     2701 ttccaaatac tagtcatatg cggccgcagc ttgaggtgtg acaggcttga gatctggcca
     2761 tacacttgag tgacaatgac atccactttg cctttctctc cacagctgcc cggcgcctac
     2821 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     2881 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtctgac
     2941 cttgatatcc agcacagtgg cggccgctcg agtagccatg gcttcccgcc ggcggtggcg
     3001 gcgcaggatg atggcacgct gcccatgtct tgtgcccagg agagcgggat ggaccgtcac
     3061 cctgcagcct gtgcttctgc taggatcaat gtgtagtaat gagtttaagg cgcgcggctc
     3121 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     3181 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     3241 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     3301 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     3361 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     3421 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3481 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3541 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3601 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtac
     3661 ctagaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt ccttggccaa
     3721 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     3781 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     3841 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     3901 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     3961 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     4021 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     4081 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     4141 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     4201 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     4261 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     4321 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     4381 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     4441 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     4501 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     4561 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     4621 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     4681 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
     4741 gcacgtacta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     4801 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4861 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4921 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4981 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     5041 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5101 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5161 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5221 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5281 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5341 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5401 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5461 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5521 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5581 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5641 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5701 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5761 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5821 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5881 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5941 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6001 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6061 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6121 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6181 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6241 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6301 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6361 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6421 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6481 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6541 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6601 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6661 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6721 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6781 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6841 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6901 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6961 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7021 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7081 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7141 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
