LOCUS       M_1NLS_Cas9h_NLS        8701 bp ds-DNA     circular     03-SEP-2014
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(7636..8493)
                     /vntifkey="4"
                     /label=A(R)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     polyA_signal    6458..6492
                     /vntifkey="25"
                     /label=pA-SV40
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        9..1924
                     /vntifkey="29"
                     /label=MesP -1
                     /ApEinfo_fwdcolor=#64ad52
                     /ApEinfo_revcolor=#64ad52
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6080..6097
                     /label=NLS
                     /ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_revcolor=#ff8040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4481..4483
                     /label=H841
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6101..6106
                     /label=2XSTOP
                     /ApEinfo_fwdcolor=#ff0080
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1937..1966
                     /label=NLS(1)
                     /ApEinfo_label=NLS
                     /ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_revcolor=#ff8040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1991..1993
                     /label=D10
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1967..6079
                     /label=Cas9h
                     /ApEinfo_fwdcolor=#b58fde
                     /ApEinfo_revcolor=#b58fde
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ggcgcgcccg gttcaacgtg acgtcccatg ccgatatcgt aaccatccgg aacctctgat
       61 gctttttcaa tatcatcttt tttgaaatcc ttcatttccg tttcatcgtc atttttgaaa
      121 gccgggttct catactcgtt tttggtgctg taaccgtttt ctgacatttt tatctcatcc
      181 aagtgcgaac cacttcaaag atggatagat acaccagatt ttattacaaa caattagcaa
      241 ttcacgaaag ttaaaaaaac gacataaaac tataaaataa aattatactt attaaatatg
      301 aagaaaaata tgcattttta atcattctat tgcaacaaat cggtattttt cgtattctct
      361 tatacgaaga ttgcatacaa gcttaacgtt tcatctgttt ccattggaat aaaatagaaa
      421 acgtcccacc gcctgcccta actttttata agcttcacaa attatcaaag tttaccgttg
      481 aactcctgat aaatgattat tcagctattt taaacccaac ttcgtttata agtgatgccg
      541 tctccttctt cgtatgaccc cgctctacta aatacatgtt tgcacactga tcctaatgaa
      601 gaccgcacct aaacctttac atagccgatg cattcacata taatgttctt tgtgcaaccg
      661 aagtcgtatt tgagcaaacc aaatatccaa cttatataag tagaccttta aaaatggacg
      721 gtgaacaaaa ctttgcgaag tcatcataat tgaaggttta tttagctctc ctattgtagg
      781 gaaagatcct atggatgctc caaaacgata aacccgactt accgaaaacg cggcggtcgt
      841 tagaccatgc ttattgataa accacataca tacaaacttt agcgcaaact cggctatatc
      901 tttacaacat tagacaaatt ctctttcatg tattttcatg ggattataaa gtgtctattt
      961 taggtaacaa gactttaata aaaatatcag tgtcccatat tgttggcggc tttgtttgcg
     1021 agtgtctctg tattgacaac gtttataatt aacttggaag taatgagtat acagcagcag
     1081 acacagaagt ttgtcggggg tatcctatga ttgtacatca tgtgagcaaa tatacttcaa
     1141 ctgctagtga ataatatttg atatacgcac aagtcgtaca aatacacgac tacaaataag
     1201 tcatacttgt cgaagtttgt attcccctgt tttcgatcaa tcttatcagc cacaaaaaat
     1261 ggaaaaattc caaaaacgta gacacccaca aagtaacata catggtaact cgtaagctaa
     1321 tacagagcac ccgtgtgaaa acgactgtcg ttttccggcc acgcgagaat aaagtaagtt
     1381 acattgattc attcactgac agtttcccac caaaactgaa gtattaatca attatgaatc
     1441 ggcagtgctt tcagtgggaa gatttcggaa aatgtgggtc taatgtcaca gtaaactttg
     1501 tgtttgtttt aaaaacaatc atcgttaaat caacatcgca attctgtcaa tcactccaca
     1561 ataaactata tggctaatgg aaacaggctg tttgtttgta tatcataaaa tacttgcgtc
     1621 gtattatctg accaaacaaa agcgttacag gaacggtcca gcttcaaaat ttgctgatgc
     1681 atcagatttt acatttgaaa tgtgattaat tacgaaaatc cagcgaatag aattgtcaca
     1741 acaagtcatt agcgacggat atttcgcctt tgaaacttaa aggcgataat gactttgccc
     1801 gtttcatgcg gcgataaacg aactaattag acacctccta cagatataat ggtaattcag
     1861 aatcgtgtgg ttatgtaatt cacaaaaaca ttttaacaaa acaggttgat ttgaaacttg
     1921 tattgcggcc gcAACCATGG CTAGCCCCAA AAAGAAGAGG AAAGTGGACA AGAAGTATTC
     1981 TATCGGACTG GACATCGGGA CTAATAGCGT CGGGTGGGCC GTGATCACTG ACGAGTACAA
     2041 GGTGCCCTCT AAGAAGTTCA AGGTGCTCGG GAACACCGAC CGGCATTCCA TCAAGAAAAA
     2101 TCTGATCGGA GCTCTCCTCT TTGATTCAGG GGAGACCGCT GAAGCAACCC GCCTCAAGCG
     2161 GACTGCTAGA CGGCGGTACA CCAGGAGGAA GAACCGGATT TGTTACCTTC AAGAGATATT
     2221 CTCCAACGAA ATGGCAAAGG TCGACGACAG CTTCTTCCAT AGGCTGGAAG AATCATTCCT
     2281 CGTGGAAGAG GATAAGAAGC ATGAACGGCA TCCCATCTTC GGTAATATCG TCGACGAGGT
     2341 GGCCTATCAC GAGAAATACC CAACCATCTA CCATCTTCGC AAAAAGCTGG TGGACTCAAC
     2401 CGACAAGGCA GACCTCCGGC TTATCTACCT GGCCCTGGCC CACATGATCA AGTTCAGAGG
     2461 CCACTTCCTG ATCGAGGGCG ACCTCAATCC TGACAATAGC GATGTGGATA AACTGTTCAT
     2521 CCAGCTGGTG CAGACTTACA ACCAGCTCTT TGAAGAGAAC CCCATCAATG CAAGCGGAGT
     2581 CGATGCCAAG GCCATTCTGT CAGCCCGGCT GTCAAAGAGC CGCAGACTTG AGAATCTTAT
     2641 CGCTCAGCTG CCGGGTGAAA AGAAAAATGG ACTGTTCGGG AACCTGATTG CTCTTTCACT
     2701 TGGGCTGACT CCCAATTTCA AGTCTAATTT CGACCTGGCA GAGGATGCCA AGCTGCAACT
     2761 GTCCAAGGAC ACCTATGATG ACGATCTCGA CAACCTCCTG GCCCAGATCG GTGACCAATA
     2821 CGCCGACCTT TTCCTTGCTG CTAAGAATCT TTCTGACGCC ATCCTGCTGT CTGACATTCT
     2881 CCGCGTGAAC ACTGAAATCA CCAAGGCCCC TCTTTCAGCT TCAATGATTA AGCGGTATGA
     2941 TGAGCACCAC CAGGACCTGA CCCTGCTTAA GGCACTCGTC CGGCAGCAGC TTCCGGAGAA
     3001 GTACAAGGAA ATCTTCTTTG ACCAGTCAAA GAATGGATAC GCCGGCTACA TCGACGGAGG
     3061 TGCCTCCCAA GAGGAATTTT ATAAGTTTAT CAAACCTATC CTTGAGAAGA TGGACGGCAC
     3121 CGAAGAGCTC CTCGTGAAAC TGAATCGGGA GGATCTGCTG CGGAAGCAGC GCACTTTCGA
     3181 CAATGGGAGC ATTCCCCACC AGATCCATCT TGGGGAGCTT CACGCCATCC TTCGGCGCCA
     3241 AGAGGACTTC TACCCCTTTC TTAAGGACAA CAGGGAGAAG ATTGAGAAAA TTCTCACTTT
     3301 CCGCATCCCC TACTACGTGG GACCCCTCGC CAGAGGAAAT AGCCGGTTTG CTTGGATGAC
     3361 CAGAAAGTCA GAAGAAACTA TCACTCCCTG GAACTTCGAA GAGGTGGTGG ACAAGGGAGC
     3421 CAGCGCTCAG TCATTCATCG AACGGATGAC TAACTTCGAT AAGAACCTCC CCAATGAGAA
     3481 GGTCCTGCCG AAACATTCCC TGCTCTACGA GTACTTTACC GTGTACAACG AGCTGACCAA
     3541 GGTGAAATAT GTCACCGAAG GGATGAGGAA GCCCGCATTC CTGTCAGGCG AACAAAAGAA
     3601 GGCAATTGTG GACCTTCTGT TCAAGACCAA TAGAAAGGTG ACCGTGAAGC AGCTGAAGGA
     3661 GGACTATTTC AAGAAAATTG AATGCTTCGA CTCTGTGGAG ATTAGCGGGG TCGAAGATCG
     3721 GTTCAACGCA AGCCTGGGTA CCTACCATGA TCTGCTTAAG ATCATCAAGG ACAAGGATTT
     3781 TCTGGACAAT GAGGAGAACG AGGACATCCT TGAGGACATT GTCCTGACTC TCACTCTGTT
     3841 CGAGGACCGG GAAATGATCG AGGAGAGGCT TAAGACCTAC GCCCATCTGT TCGACGATAA
     3901 AGTGATGAAG CAACTTAAAC GGAGAAGATA TACCGGATGG GGACGCCTTA GCCGCAAACT
     3961 CATCAACGGA ATCCGGGACA AACAGAGCGG AAAGACCATT CTTGATTTCC TTAAGAGCGA
     4021 CGGATTCGCT AATCGCAACT TCATGCAACT TATCCATGAT GATTCCCTGA CCTTTAAGGA
     4081 GGACATCCAG AAGGCCCAAG TGTCTGGACA AGGTGACTCA CTGCACGAGC ATATCGCAAA
     4141 TCTGGCTGGT TCACCCGCTA TTAAGAAGGG TATTCTCCAG ACCGTGAAAG TCGTGGACGA
     4201 GCTGGTCAAG GTGATGGGTC GCCATAAACC AGAGAACATT GTCATCGAGA TGGCCAGGGA
     4261 AAACCAGACT ACCCAGAAGG GACAGAAGAA CAGCAGGGAG CGGATGAAAA GAATTGAGGA
     4321 AGGGATTAAG GAGCTCGGGT CACAGATCCT TAAAGAGCAC CCGGTGGAAA ACACCCAGCT
     4381 TCAGAATGAG AAGCTCTATC TGTACTACCT TCAAAATGGA CGCGATATGT ATGTGGACCA
     4441 AGAGCTTGAT ATCAACAGGC TCTCAGACTA CGACGTGGAC CACATCGTCC CTCAGAGCTT
     4501 CCTCAAAGAC GACTCAATTG ACAATAAGGT GCTGACTCGC TCAGACAAGA ACCGGGGAAA
     4561 GTCAGATAAC GTGCCCTCAG AGGAAGTCGT GAAAAAGATG AAGAACTATT GGCGCCAGCT
     4621 TCTGAACGCA AAGCTGATCA CTCAGCGGAA GTTCGACAAT CTCACTAAGG CTGAGAGGGG
     4681 CGGACTGAGC GAACTGGACA AAGCAGGATT CATTAAACGG CAACTTGTGG AGACTCGGCA
     4741 GATTACTAAA CATGTCGCCC AAATCCTTGA CTCACGCATG AATACCAAGT ACGACGAAAA
     4801 CGACAAACTT ATCCGCGAGG TGAAGGTGAT TACCCTGAAG TCCAAGCTGG TCAGCGATTT
     4861 CAGAAAGGAC TTTCAATTCT ACAAAGTGCG GGAGATCAAT AACTATCATC ATGCTCATGA
     4921 CGCATATCTG AATGCCGTGG TGGGAACCGC CCTGATCAAG AAGTACCCAA AGCTGGAAAG
     4981 CGAGTTCGTG TACGGAGACT ACAAGGTCTA CGACGTGCGC AAGATGATTG CCAAATCTGA
     5041 GCAGGAGATC GGAAAGGCCA CCGCAAAGTA CTTCTTCTAC AGCAACATCA TGAATTTCTT
     5101 CAAGACCGAA ATCACCCTTG CAAACGGTGA GATCCGGAAG AGGCCGCTCA TCGAGACTAA
     5161 TGGGGAGACT GGCGAAATCG TGTGGGACAA GGGCAGAGAT TTCGCTACCG TGCGCAAAGT
     5221 GCTTTCTATG CCTCAAGTGA ACATCGTGAA GAAAACCGAG GTGCAAACCG GAGGCTTTTC
     5281 TAAGGAATCA ATCCTCCCCA AGCGCAACTC CGACAAGCTC ATTGCAAGGA AGAAGGATTG
     5341 GGACCCTAAG AAGTACGGCG GATTCGATTC ACCAACTGTG GCTTATTCTG TCCTGGTCGT
     5401 GGCTAAGGTG GAAAAAGGAA AGTCTAAGAA GCTCAAGAGC GTGAAGGAAC TGCTGGGTAT
     5461 CACCATTATG GAGCGCAGCT CCTTCGAGAA GAACCCAATT GACTTTCTCG AAGCCAAAGG
     5521 TTACAAGGAA GTCAAGAAGG ACCTTATCAT CAAGCTCCCA AAGTATAGCC TGTTCGAACT
     5581 GGAGAATGGG CGGAAGCGGA TGCTCGCCTC CGCTGGCGAA CTTCAGAAGG GTAATGAGCT
     5641 GGCTCTCCCC TCCAAGTACG TGAATTTCCT CTACCTTGCA AGCCATTACG AGAAGCTGAA
     5701 GGGGAGCCCC GAGGACAACG AGCAAAAGCA ACTGTTTGTG GAGCAGCATA AGCATTATCT
     5761 GGACGAGATC ATTGAGCAGA TTTCCGAGTT TTCTAAACGC GTCATTCTCG CTGATGCCAA
     5821 CCTCGATAAA GTCCTTAGCG CATACAATAA GCACAGAGAC AAACCAATTC GGGAGCAGGC
     5881 TGAGAATATC ATCCACCTGT TCACCCTCAC CAATCTTGGT GCCCCTGCCG CATTCAAGTA
     5941 CTTCGACACC ACCATCGACC GGAAACGCTA TACCTCCACC AAAGAAGTGC TGGACGCCAC
     6001 CCTCATCCAC CAGAGCATCA CCGGACTTTA CGAAACTCGG ATTGACCTCT CACAGCTCGG
     6061 AGGGGATGAG GGAGCTCCCA AGAAAAAGCG CAAGGTAGGT TAATGAgaat tccagctgag
     6121 cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc
     6181 atgtctgccc gtatttcgcg taaggaaatc cattatgtac tatttaaaaa acacaaactt
     6241 ttggatgttc ggtttattct ttttctttta cttttttatc atgggagcct acttcccgtt
     6301 tttcccgatt tggctacatg acatcaacca tatcagcaaa agtgatacgg gtattatttt
     6361 tgccgctatt tctctgttct cgctattatt ccaaccgctg tttggtctgc tttctgacaa
     6421 actcggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6481 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6541 gtatcttatc atgtctggat cgacaaagtc aaagcggcca tcagatctgc cggtctccct
     6601 atagtgagtc gtattaattt cgataagcca ggttaacctg cattaatgaa tcggccaacg
     6661 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6721 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6781 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6841 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6901 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6961 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7021 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
     7081 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7141 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7201 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7261 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     7321 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7381 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7441 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7501 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7561 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7621 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7681 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7741 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7801 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7861 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7921 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7981 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8041 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8101 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8161 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8221 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8281 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8341 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8401 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8461 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8521 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8581 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     8641 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcccttac gtattaatta
     8701 a     
//
