LOCUS       2mU6_COEa_34NT_S        3678 bp ds-DNA     circular     31-MAY-2014
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2634..3491
                     /label=Amp(R)
                     /ApEinfo_fwdcolor=#9c6378
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     polyA_signal    1456..1490
                     /vntifkey="25"
                     /label=pA-SV40
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    30..988
                     /vntifkey="21"
                     /label=U6\promoter
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#b89aa6
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    989..991
                     /label=EcoRV remnant
                     /ApEinfo_fwdcolor=#808080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1592..1592
                     /label=BsaI mutated
                     /ApEinfo_fwdcolor=#c3063c
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2775..2775
                     /label=BsaI mutated(1)
                     /ApEinfo_label=BsaI mutated
                     /ApEinfo_fwdcolor=#c3063c
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1012..1104
                     /label=sgRNA F+E
                     /ApEinfo_fwdcolor=#730e8c
                     /ApEinfo_revcolor=#730e8c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    992..1011
                     /label=COEa.774
                     /ApEinfo_fwdcolor=#408080
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 aggcccttac gtattaatta aggcgcgcct ggcgggtgta ttaaaccact aaacaaacaa
       61 ttgccccaag ctctcttcac aattataaac actataatgt ttggacaaga gattagcgtg
      121 gctgtgacga gaactctcaa aggcttggtg taattgatat tttataagaa gcagattaaa
      181 cttcaataca gtttacacct catttacaaa aaattggctg ccaaaatcgc taatttacac
      241 atatttaaaa caatttcaac acatatacac agtatagtat aactgcataa taaacaaata
      301 catctaacag acactcacta atctgccata acaagcttca aaaacttaaa ctcgaaattt
      361 tagtgaatct ttttttttaa atgaatattt tatttaaaaa gttaaaaata ttacagttca
      421 ggtataggtt tacacctaat ctttaataat ccgaactaaa ttttaactat ttagaaactt
      481 tttcaaccaa agtttaaaaa aatagatttt tcgcacgcta aaactatcat ttacacaaaa
      541 aaatgcaaca aaatgcagaa aaaaattaca ttagagttta ggttagttac ctgctaatca
      601 atataaacta acttcccgca taatattcat ttaaaattag caataatcac gttttacgct
      661 aaaatttgtg taaaactaaa cttcgtcctt tgtcaaggag aaaatttgac tcaaaagctg
      721 cgcgcgcagg ggagatcccc aagcgagtgt ttgttacatc ataatcatgt ggaaaaatcc
      781 cctaataagt aaaaatatat ttttttttaa tttttggggc gaataaaccg ctttttatgt
      841 ctaaaaacgc caaaaatgga tcgcgcgagc ccaaaaacgc acaaataacg tacagacagt
      901 gtctctgcgt acacagacgg tatttcccct ttaaattgag aactagactt aagcacgctt
      961 ataagtctgg aaggcatccg atggtataga tGCTGAGGGT TGGACAACAG GGTTTAAGAG
     1021 CTATGCTGGA AACAGCATAG CAAGTTTAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG
     1081 TGGCACCGAG TCGGTGCTTT TTTTGAATTC cagctgagcg ccggtcgcta ccattaccag
     1141 ttggtctggt gtcaaaaata ataataaccg ggcaggccat gtctgcccgt atttcgcgta
     1201 aggaaatcca ttatgtacta tttaaaaaac acaaactttt ggatgttcgg tttattcttt
     1261 ttcttttact tttttatcat gggagcctac ttcccgtttt tcccgatttg gctacatgac
     1321 atcaaccata tcagcaaaag tgatacgggt attatttttg ccgctatttc tctgttctcg
     1381 ctattattcc aaccgctgtt tggtctgctt tctgacaaac tcggaacttg tttattgcag
     1441 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     1501 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg
     1561 acaaagtcaa agcggccatc agatctgccg gActccctat agtgagtcgt attaatttcg
     1621 ataagccagg ttaacctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1681 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1741 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1801 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1861 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1921 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1981 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2041 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     2101 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2161 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2221 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2281 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     2341 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2401 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2461 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2521 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2581 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2641 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2701 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2761 atgataccgc gagaTccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2821 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2881 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     2941 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3001 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3061 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3121 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3181 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3241 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3301 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3361 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3421 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3481 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3541 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3601 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     3661 aaaaataggc gtatcacg    
//
