LOCUS       pAAV-EF1a-EYFP-W        6067 bp    DNA     circular     20-MAY-2010
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|538328968|
COMMENT     VNTDBDATE|538763081|
COMMENT     VNTNAME|pAAV-EF1a-EYFP-WPRE|
COMMENT     VNTAUTHORNAME|William Hendriks|
COMMENT     VNTAUTHOREML|hendriks.william@mgh.harvard.edu|
COMMENT     VNTAUTHORNAME|Feng Zhang|
COMMENT     VNTAUTHOREML|zhang72@fas.harvard.edu|
FEATURES             Location/Qualifiers
     gene            1..141
                     /dnas_title="L-ITR"
                     /gene="L-ITR"
                     /vntifkey="21"
                     /label=L-ITR
     gene            3511..3989
                     /dnas_title="hGH polyA"
                     /gene="hGH polyA"
                     /vntifkey="25"
                     /label=hGH polyA
     gene            4029..4169
                     /dnas_title="R-ITR"
                     /gene="R-ITR"
                     /vntifkey="21"
                     /label=R-ITR
     exon            4261..4567
                     /dnas_title="f1 Origin"
                     /gene="f1 Origin"
                     /vntifkey="33"
                     /label=f1 Origin
     exon            5086..5943
                     /dnas_title="AmpR"
                     /gene="AmpR"
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      6094..6761
                     /dnas_title="pUC ori"
                     /gene="pUC ori"
                     /vntifkey="33"
                     /label=pUC ori
     gene            2883..3491
                     /dnas_title="WPRE"
                     /gene="WPRE"
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    1726..1791
                     /dnas_title="GSG-P2A"
                     /vntifkey="21"
                     /label=GSG-P2A
     CDS             1792..2838
                     /label=Cre
     CDS             2839..2865
                     /dnas_title="HA"
                     /vntifkey="4"
                     /label=HA
     misc_feature    787..1719
                     /note="Renilla Luciferase"
     misc_feature    520..774
                     /note="EFs-NS"
     promoter        156..404
                     /dnas_title="Human U6 Promoter"
                     /vntifkey="29"
                     /label=Human U6 Promoter
     misc_feature    405..507
                     /note="sgRNA"
     misc_feature    406..425
                     /note="LacZ-1"
ORIGIN      
          1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
         61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
        121 actccatcac taggggttcc tgcggccgca cgcgtgaggg cctatttccc atgattcctt
        181 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
        241 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
        301 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
        361 tcgatttctt ggctttatat atcttGTGGA AAGGACGAAA CACCgTGCGA ATACGCCCAC
        421 GCGATgtttt agagctaGAA Atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
        481 aaagtggcac cgagtcggtg cTTTTTTtct agaggtacca ggtcttgaaa ggagtgggaa
        541 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
        601 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
        661 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
        721 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggcgtacg
        781 gccaccATGA CTTCGAAAGT TTATGATCCA GAACAAAGGA AACGGATGAT AACTGGTCCG
        841 CAGTGGTGGG CCAGATGTAA ACAAATGAAT GTTCTTGATT CATTTATTAA TTATTATGAT
        901 TCAGAAAAAC ATGCAGAAAA TGCTGTTATT TTTTTACATG GTAACGCGGC CTCTTCTTAT
        961 TTATGGCGAC ATGTTGTGCC ACATATTGAG CCAGTAGCGC GGTGTATTAT ACCAGACCTT
       1021 ATTGGTATGG GCAAATCAGG CAAATCTGGT AATGGTTCTT ATAGGTTACT TGATCATTAC
       1081 AAATATCTTA CTGCATGGTT TGAACTTCTT AATTTACCAA AGAAGATCAT TTTTGTCGGC
       1141 CATGATTGGG GTGCTTGTTT GGCATTTCAT TATAGCTATG AGCATCAAGA TAAGATCAAA
       1201 GCAATAGTTC ACGCTGAAAG TGTAGTAGAT GTGATTGAAT CATGGGATGA ATGGCCTGAT
       1261 ATTGAAGAAG ATATTGCGTT GATCAAATCT GAAGAAGGAG AAAAAATGGT TTTGGAGAAT
       1321 AACTTCTTCG TGGAAACCAT GTTGCCATCA AAAATCATGA GAAAGTTAGA ACCAGAAGAA
       1381 TTTGCAGCAT ATCTTGAACC ATTCAAAGAG AAAGGTGAAG TTCGTCGTCC AACATTATCA
       1441 TGGCCTCGTG AAATCCCGTT AGTAAAAGGT GGTAAACCTG ACGTTGTACA AATTGTTAGG
       1501 AATTATAATG CTTATCTACG TGCAAGTGAT GATTTACCAA AAATGTTTAT TGAATCGGAC
       1561 CCAGGATTCT TTTCCAATGC TATTGTTGAA GGTGCCAAGA AGTTTCCTAA TACTGAATTT
       1621 GTCAAAGTAA AAGGTCTTCA TTTTTCGCAA GAAGATGCAC CTGATGAAAT GGGAAAATAT
       1681 ATCAAATCGT TCGTTGAGCG AGTTCTCAAA AATGAACAAG CTAGCggaag cggagccact
       1741 aacttctccc tgttgaaaca agcaggggat gtcgaagaga atcccgggcc acccaagaag
       1801 aagaggaagg tgtccaatct cctgactgtt caccagaacc tccctgcgct gccagtagat
       1861 gccactagcg atgaggtcag gaaaaatctc atggatatgt ttagggatag acaggcgttt
       1921 tctgaacaca cctggaaaat gctgcttagc gtgtgccgat cctgggcagc ctggtgtaag
       1981 ctgaacaatc gcaaatggtt ccccgccgag ccggaggacg tgcgcgatta cctgctgtat
       2041 ctccaggcaa gagggctggc tgtcaagact atccagcagc acttgggcca actgaatatg
       2101 ctgcatcgac gcagcgggct cccccggcct agcgattcaa acgcagtctc ccttgttatg
       2161 aggagaatta gaaaggaaaa cgtagatgcg ggtgagaggg ctaagcaggc tctcgctttt
       2221 gagcggactg atttcgacca ggtcagatcc ctgatggaga acagcgatcg gtgccaggac
       2281 atcaggaacc tcgcatttct gggaattgca tataacacac ttctgcgcat agctgagatc
       2341 gcccggatca gagtgaaaga catcagtcga acggacggcg gccggatgct tattcatatt
       2401 ggacgcacaa agacattggt cagcaccgct ggcgttgaaa aggccttgtc cctgggcgta
       2461 acgaagctgg tggaaagatg gatctcagtg tccggcgtgg ctgacgaccc taataattac
       2521 ttgttctgtc gagtgagaaa aaacggagtc gccgcgccct ctgccaccag ccaattgagt
       2581 acacgggccc ttgaagggat ctttgaggca acccaccgac tcatatacgg agccaaggat
       2641 gacagtggcc agaggtatct cgcctggtca ggtcattctg ctagggtggg ggccgcacga
       2701 gacatggcgc gggcaggagt ctccatacca gagattatgc aagctggagg ttggacaaat
       2761 gtgaacatcg ttatgaacta tatccgcaat cttgactctg aaaccggggc catggtgaga
       2821 ctgctcgaag atggtgacta cccatacgat gttccagatt acgctTAAGA ATTCgatatc
       2881 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
       2941 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
       3001 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
       3061 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
       3121 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
       3181 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
       3241 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
       3301 ccttggctgc tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
       3361 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
       3421 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
       3481 catcgatacc gagcgctgct cgagagatct acgggtggca tccctgtgac ccctccccag
       3541 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
       3601 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
       3661 tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg
       3721 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
       3781 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
       3841 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
       3901 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
       3961 cgtgaaccac tgctcccttc cctgtccttc tgattttgta ggtaaccacg tgcggaccga
       4021 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
       4081 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
       4141 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
       4201 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
       4261 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
       4321 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
       4381 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
       4441 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
       4501 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
       4561 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
       4621 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
       4681 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
       4741 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
       4801 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
       4861 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
       4921 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
       4981 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       5041 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
       5101 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
       5161 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
       5221 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
       5281 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
       5341 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
       5401 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
       5461 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
       5521 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
       5581 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
       5641 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
       5701 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
       5761 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
       5821 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
       5881 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
       5941 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
       6001 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
       6061 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
       6121 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
       6181 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
       6241 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
       6301 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
       6361 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
       6421 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
       6481 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
       6541 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
       6601 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
       6661 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
       6721 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//

