LOCUS       pAAV-EF1a-EYFP-W        6067 bp    DNA     circular     20-MAY-2010
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|538328968|
COMMENT     VNTDBDATE|538763081|
COMMENT     VNTNAME|pAAV-EF1a-EYFP-WPRE|
COMMENT     VNTAUTHORNAME|William Hendriks|
COMMENT     VNTAUTHOREML|hendriks.william@mgh.harvard.edu|
COMMENT     VNTAUTHORNAME|Feng Zhang|
COMMENT     VNTAUTHOREML|zhang72@fas.harvard.edu|
FEATURES             Location/Qualifiers
     gene            1..141
                     /dnas_title="L-ITR"
                     /gene="L-ITR"
                     /vntifkey="21"
                     /label=L-ITR
     gene            3508..3986
                     /dnas_title="hGH polyA"
                     /gene="hGH polyA"
                     /vntifkey="25"
                     /label=hGH polyA
     gene            4026..4166
                     /dnas_title="R-ITR"
                     /gene="R-ITR"
                     /vntifkey="21"
                     /label=R-ITR
     exon            4258..4564
                     /dnas_title="f1 Origin"
                     /gene="f1 Origin"
                     /vntifkey="33"
                     /label=f1 Origin
     exon            5083..5940
                     /dnas_title="AmpR"
                     /gene="AmpR"
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      6091..6758
                     /dnas_title="pUC ori"
                     /gene="pUC ori"
                     /vntifkey="33"
                     /label=pUC ori
     gene            2880..3488
                     /dnas_title="WPRE"
                     /gene="WPRE"
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    1723..1788
                     /dnas_title="GSG-P2A"
                     /vntifkey="21"
                     /label=GSG-P2A
     CDS             1789..2835
                     /label=Cre
     CDS             2836..2862
                     /dnas_title="HA"
                     /vntifkey="4"
                     /label=HA
     misc_feature    784..1716
                     /note="Renilla Luciferase"
     misc_feature    517..771
                     /note="EFs-NS"
     promoter        156..404
                     /dnas_title="Human U6 Promoter"
                     /vntifkey="29"
                     /label=Human U6 Promoter
     misc_feature    405..504
                     /note="sgRNA backbone (SapI)"
     misc_feature    415..421
                     /note="SapI"
     misc_feature    406..412
                     /note="SapI"
ORIGIN      
          1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
         61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
        121 actccatcac taggggttcc tgcggccgca cgcgtgaggg cctatttccc atgattcctt
        181 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
        241 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
        301 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
        361 tcgatttctt ggctttatat atcttGTGGA AAGGACGAAA CACCggaaga gcgagctctt
        421 ctgttttaga gctaGAAAta gcaagttaaa ataaggctag tccgttatca acttgaaaaa
        481 gtggcaccga gtcggtgcTT TTTTtctaga ggtaccaggt cttgaaagga gtgggaattg
        541 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
        601 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
        661 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
        721 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gcgtacggcc
        781 accATGACTT CGAAAGTTTA TGATCCAGAA CAAAGGAAAC GGATGATAAC TGGTCCGCAG
        841 TGGTGGGCCA GATGTAAACA AATGAATGTT CTTGATTCAT TTATTAATTA TTATGATTCA
        901 GAAAAACATG CAGAAAATGC TGTTATTTTT TTACATGGTA ACGCGGCCTC TTCTTATTTA
        961 TGGCGACATG TTGTGCCACA TATTGAGCCA GTAGCGCGGT GTATTATACC AGACCTTATT
       1021 GGTATGGGCA AATCAGGCAA ATCTGGTAAT GGTTCTTATA GGTTACTTGA TCATTACAAA
       1081 TATCTTACTG CATGGTTTGA ACTTCTTAAT TTACCAAAGA AGATCATTTT TGTCGGCCAT
       1141 GATTGGGGTG CTTGTTTGGC ATTTCATTAT AGCTATGAGC ATCAAGATAA GATCAAAGCA
       1201 ATAGTTCACG CTGAAAGTGT AGTAGATGTG ATTGAATCAT GGGATGAATG GCCTGATATT
       1261 GAAGAAGATA TTGCGTTGAT CAAATCTGAA GAAGGAGAAA AAATGGTTTT GGAGAATAAC
       1321 TTCTTCGTGG AAACCATGTT GCCATCAAAA ATCATGAGAA AGTTAGAACC AGAAGAATTT
       1381 GCAGCATATC TTGAACCATT CAAAGAGAAA GGTGAAGTTC GTCGTCCAAC ATTATCATGG
       1441 CCTCGTGAAA TCCCGTTAGT AAAAGGTGGT AAACCTGACG TTGTACAAAT TGTTAGGAAT
       1501 TATAATGCTT ATCTACGTGC AAGTGATGAT TTACCAAAAA TGTTTATTGA ATCGGACCCA
       1561 GGATTCTTTT CCAATGCTAT TGTTGAAGGT GCCAAGAAGT TTCCTAATAC TGAATTTGTC
       1621 AAAGTAAAAG GTCTTCATTT TTCGCAAGAA GATGCACCTG ATGAAATGGG AAAATATATC
       1681 AAATCGTTCG TTGAGCGAGT TCTCAAAAAT GAACAAGCTA GCggaagcgg agccactaac
       1741 ttctccctgt tgaaacaagc aggggatgtc gaagagaatc ccgggccacc caagaagaag
       1801 aggaaggtgt ccaatctcct gactgttcac cagaacctcc ctgcgctgcc agtagatgcc
       1861 actagcgatg aggtcaggaa aaatctcatg gatatgttta gggatagaca ggcgttttct
       1921 gaacacacct ggaaaatgct gcttagcgtg tgccgatcct gggcagcctg gtgtaagctg
       1981 aacaatcgca aatggttccc cgccgagccg gaggacgtgc gcgattacct gctgtatctc
       2041 caggcaagag ggctggctgt caagactatc cagcagcact tgggccaact gaatatgctg
       2101 catcgacgca gcgggctccc ccggcctagc gattcaaacg cagtctccct tgttatgagg
       2161 agaattagaa aggaaaacgt agatgcgggt gagagggcta agcaggctct cgcttttgag
       2221 cggactgatt tcgaccaggt cagatccctg atggagaaca gcgatcggtg ccaggacatc
       2281 aggaacctcg catttctggg aattgcatat aacacacttc tgcgcatagc tgagatcgcc
       2341 cggatcagag tgaaagacat cagtcgaacg gacggcggcc ggatgcttat tcatattgga
       2401 cgcacaaaga cattggtcag caccgctggc gttgaaaagg ccttgtccct gggcgtaacg
       2461 aagctggtgg aaagatggat ctcagtgtcc ggcgtggctg acgaccctaa taattacttg
       2521 ttctgtcgag tgagaaaaaa cggagtcgcc gcgccctctg ccaccagcca attgagtaca
       2581 cgggcccttg aagggatctt tgaggcaacc caccgactca tatacggagc caaggatgac
       2641 agtggccaga ggtatctcgc ctggtcaggt cattctgcta gggtgggggc cgcacgagac
       2701 atggcgcggg caggagtctc cataccagag attatgcaag ctggaggttg gacaaatgtg
       2761 aacatcgtta tgaactatat ccgcaatctt gactctgaaa ccggggccat ggtgagactg
       2821 ctcgaagatg gtgactaccc atacgatgtt ccagattacg ctTAAGAATT Cgatatcaag
       2881 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
       2941 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
       3001 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
       3061 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
       3121 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
       3181 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
       3241 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
       3301 tggctgctcg cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
       3361 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
       3421 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
       3481 cgataccgag cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc
       3541 ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa
       3601 gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg
       3661 tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac
       3721 caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag
       3781 cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca
       3841 gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca
       3901 actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt
       3961 gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg
       4021 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
       4081 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
       4141 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
       4201 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
       4261 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
       4321 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
       4381 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
       4441 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
       4501 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
       4561 acaacactca accctatctc gggctattct tttgatttat aagggatttt gccgatttcg
       4621 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
       4681 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
       4741 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
       4801 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
       4861 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
       4921 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
       4981 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
       5041 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
       5101 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
       5161 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
       5221 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
       5281 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
       5341 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
       5401 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
       5461 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
       5521 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
       5581 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
       5641 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
       5701 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
       5761 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
       5821 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
       5881 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
       5941 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
       6001 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
       6061 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
       6121 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
       6181 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
       6241 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
       6301 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
       6361 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
       6421 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
       6481 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
       6541 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
       6601 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
       6661 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
       6721 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//

