LOCUS       pAAV-EF1a-EYFP-W        6067 bp    DNA     circular     20-MAY-2010
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|538328968|
COMMENT     VNTDBDATE|538763081|
COMMENT     VNTNAME|pAAV-EF1a-EYFP-WPRE|
COMMENT     VNTAUTHORNAME|William Hendriks|
COMMENT     VNTAUTHOREML|hendriks.william@mgh.harvard.edu|
COMMENT     VNTAUTHORNAME|Feng Zhang|
COMMENT     VNTAUTHOREML|zhang72@fas.harvard.edu|
FEATURES             Location/Qualifiers
     gene            1..141
                     /dnas_title="L-ITR"
                     /gene="L-ITR"
                     /vntifkey="21"
                     /label=L-ITR
     gene            3141..3619
                     /dnas_title="hGH polyA"
                     /gene="hGH polyA"
                     /vntifkey="25"
                     /label=hGH polyA
     gene            3659..3799
                     /dnas_title="R-ITR"
                     /gene="R-ITR"
                     /vntifkey="21"
                     /label=R-ITR
     exon            3891..4197
                     /dnas_title="f1 Origin"
                     /gene="f1 Origin"
                     /vntifkey="33"
                     /label=f1 Origin
     exon            4716..5573
                     /dnas_title="AmpR"
                     /gene="AmpR"
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      5724..6391
                     /dnas_title="pUC ori"
                     /gene="pUC ori"
                     /vntifkey="33"
                     /label=pUC ori
     gene            2513..3121
                     /dnas_title="WPRE"
                     /gene="WPRE"
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    590..1406
                     /note="CBh"
     CDS             1422..2468
                     /label=Cre
     CDS             2469..2495
                     /dnas_title="HA"
                     /vntifkey="4"
                     /label=HA
     promoter        156..404
                     /dnas_title="Human U6 Promoter"
                     /vntifkey="29"
                     /label=Human U6 Promoter
     misc_feature    405..506
                     /note="sgRNA"
     misc_feature    405..424
                     /note="sgNeuN-1"
ORIGIN      
          1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
         61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
        121 actccatcac taggggttcc tgcggccgca cgcgtgaggg cctatttccc atgattcctt
        181 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
        241 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
        301 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
        361 tcgatttctt ggctttatat atcttGTGGA AAGGACGAAA CACCGTCGGG GTCCCTGAAC
        421 CGGAgtttta gagctaGAAA tagcaagtta aaataaggct agtccgttat caacttgaaa
        481 aagtggcacc gagtcggtgc TTTTTTgttt tagagctaga aatagcaagt taaaataagg
        541 ctagtccgta gcgcgtgcgc caattctgca gacaaattct agaggtaccc gttacataac
        601 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
        661 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
        721 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
        781 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
        841 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
        901 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
        961 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg
       1021 cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg
       1081 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
       1141 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgacgctg ccttcgcccc
       1201 gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
       1261 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagct gagcaagagg
       1321 taagggttta agggatggtt ggttggtggg gtattaatgt ttaattacct ggagcacctg
       1381 cctgaaatca ctttttttca ggttGGACCG GTgccaccat gcccaagaag aagaggaagg
       1441 tgtccaatct cctgactgtt caccagaacc tccctgcgct gccagtagat gccactagcg
       1501 atgaggtcag gaaaaatctc atggatatgt ttagggatag acaggcgttt tctgaacaca
       1561 cctggaaaat gctgcttagc gtgtgccgat cctgggcagc ctggtgtaag ctgaacaatc
       1621 gcaaatggtt ccccgccgag ccggaggacg tgcgcgatta cctgctgtat ctccaggcaa
       1681 gagggctggc tgtcaagact atccagcagc acttgggcca actgaatatg ctgcatcgac
       1741 gcagcgggct cccccggcct agcgattcaa acgcagtctc ccttgttatg aggagaatta
       1801 gaaaggaaaa cgtagatgcg ggtgagaggg ctaagcaggc tctcgctttt gagcggactg
       1861 atttcgacca ggtcagatcc ctgatggaga acagcgatcg gtgccaggac atcaggaacc
       1921 tcgcatttct gggaattgca tataacacac ttctgcgcat agctgagatc gcccggatca
       1981 gagtgaaaga catcagtcga acggacggcg gccggatgct tattcatatt ggacgcacaa
       2041 agacattggt cagcaccgct ggcgttgaaa aggccttgtc cctgggcgta acgaagctgg
       2101 tggaaagatg gatctcagtg tccggcgtgg ctgacgaccc taataattac ttgttctgtc
       2161 gagtgagaaa aaacggagtc gccgcgccct ctgccaccag ccaattgagt acacgggccc
       2221 ttgaagggat ctttgaggca acccaccgac tcatatacgg agccaaggat gacagtggcc
       2281 agaggtatct cgcctggtca ggtcattctg ctagggtggg ggccgcacga gacatggcgc
       2341 gggcaggagt ctccatacca gagattatgc aagctggagg ttggacaaat gtgaacatcg
       2401 ttatgaacta tatccgcaat cttgactctg aaaccggggc catggtgaga ctgctcgaag
       2461 atggtgacta cccatacgat gttccagatt acgctTAAGA ATTCgatatc aagcttatcg
       2521 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
       2581 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
       2641 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
       2701 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
       2761 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
       2821 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
       2881 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
       2941 tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
       3001 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
       3061 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
       3121 gagcgctgct cgagagatct acgggtggca tccctgtgac ccctccccag tgcctctcct
       3181 ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat
       3241 cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag
       3301 caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg
       3361 gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct
       3421 cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt
       3481 tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta
       3541 atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac
       3601 tgctcccttc cctgtccttc tgattttgta ggtaaccacg tgcggaccga gcggccgcag
       3661 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
       3721 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
       3781 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
       3841 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
       3901 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
       3961 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
       4021 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
       4081 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
       4141 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
       4201 tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt
       4261 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
       4321 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
       4381 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
       4441 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
       4501 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
       4561 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
       4621 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
       4681 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
       4741 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
       4801 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
       4861 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
       4921 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
       4981 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
       5041 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
       5101 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
       5161 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
       5221 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
       5281 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
       5341 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
       5401 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
       5461 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
       5521 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
       5581 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
       5641 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
       5701 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
       5761 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
       5821 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
       5881 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
       5941 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
       6001 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
       6061 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
       6121 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
       6181 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
       6241 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
       6301 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
       6361 ggttcctggc cttttgctgg ccttttgctc acatgt
//

