LOCUS       pAAV-EF1a-EYFP-W        6067 bp    DNA     circular     20-MAY-2010
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|538328968|
COMMENT     VNTDBDATE|538763081|
COMMENT     VNTNAME|pAAV-EF1a-EYFP-WPRE|
COMMENT     VNTAUTHORNAME|William Hendriks|
COMMENT     VNTAUTHOREML|hendriks.william@mgh.harvard.edu|
COMMENT     VNTAUTHORNAME|Feng Zhang|
COMMENT     VNTAUTHOREML|zhang72@fas.harvard.edu|
FEATURES             Location/Qualifiers
     gene            1..141
                     /dnas_title="L-ITR"
                     /gene="L-ITR"
                     /vntifkey="21"
                     /label=L-ITR
     gene            3142..3620
                     /dnas_title="hGH polyA"
                     /gene="hGH polyA"
                     /vntifkey="25"
                     /label=hGH polyA
     gene            3660..3800
                     /dnas_title="R-ITR"
                     /gene="R-ITR"
                     /vntifkey="21"
                     /label=R-ITR
     exon            3892..4198
                     /dnas_title="f1 Origin"
                     /gene="f1 Origin"
                     /vntifkey="33"
                     /label=f1 Origin
     exon            4717..5574
                     /dnas_title="AmpR"
                     /gene="AmpR"
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      5725..6392
                     /dnas_title="pUC ori"
                     /gene="pUC ori"
                     /vntifkey="33"
                     /label=pUC ori
     gene            2514..3122
                     /dnas_title="WPRE"
                     /gene="WPRE"
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    591..1407
                     /note="CBh"
     CDS             1423..2469
                     /label=Cre
     CDS             2470..2496
                     /dnas_title="HA"
                     /vntifkey="4"
                     /label=HA
     promoter        156..404
                     /dnas_title="Human U6 Promoter"
                     /vntifkey="29"
                     /label=Human U6 Promoter
     misc_feature    405..507
                     /note="sgRNA"
     misc_feature    406..425
                     /note="sgLacZ-1"
ORIGIN      
          1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
         61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
        121 actccatcac taggggttcc tgcggccgca cgcgtgaggg cctatttccc atgattcctt
        181 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
        241 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
        301 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
        361 tcgatttctt ggctttatat atcttGTGGA AAGGACGAAA CACCGTGCGA ATACGCCCAC
        421 GCGATgtttt agagctaGAA Atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
        481 aaagtggcac cgagtcggtg cTTTTTTgtt ttagagctag aaatagcaag ttaaaataag
        541 gctagtccgt agcgcgtgcg ccaattctgc agacaaattc tagaggtacc cgttacataa
        601 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
        661 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
        721 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
        781 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
        841 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag
        901 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
        961 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc
       1021 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
       1081 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
       1141 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgacgct gccttcgccc
       1201 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
       1261 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc tgagcaagag
       1321 gtaagggttt aagggatggt tggttggtgg ggtattaatg tttaattacc tggagcacct
       1381 gcctgaaatc actttttttc aggttGGACC GGTgccacca tgcccaagaa gaagaggaag
       1441 gtgtccaatc tcctgactgt tcaccagaac ctccctgcgc tgccagtaga tgccactagc
       1501 gatgaggtca ggaaaaatct catggatatg tttagggata gacaggcgtt ttctgaacac
       1561 acctggaaaa tgctgcttag cgtgtgccga tcctgggcag cctggtgtaa gctgaacaat
       1621 cgcaaatggt tccccgccga gccggaggac gtgcgcgatt acctgctgta tctccaggca
       1681 agagggctgg ctgtcaagac tatccagcag cacttgggcc aactgaatat gctgcatcga
       1741 cgcagcgggc tcccccggcc tagcgattca aacgcagtct cccttgttat gaggagaatt
       1801 agaaaggaaa acgtagatgc gggtgagagg gctaagcagg ctctcgcttt tgagcggact
       1861 gatttcgacc aggtcagatc cctgatggag aacagcgatc ggtgccagga catcaggaac
       1921 ctcgcatttc tgggaattgc atataacaca cttctgcgca tagctgagat cgcccggatc
       1981 agagtgaaag acatcagtcg aacggacggc ggccggatgc ttattcatat tggacgcaca
       2041 aagacattgg tcagcaccgc tggcgttgaa aaggccttgt ccctgggcgt aacgaagctg
       2101 gtggaaagat ggatctcagt gtccggcgtg gctgacgacc ctaataatta cttgttctgt
       2161 cgagtgagaa aaaacggagt cgccgcgccc tctgccacca gccaattgag tacacgggcc
       2221 cttgaaggga tctttgaggc aacccaccga ctcatatacg gagccaagga tgacagtggc
       2281 cagaggtatc tcgcctggtc aggtcattct gctagggtgg gggccgcacg agacatggcg
       2341 cgggcaggag tctccatacc agagattatg caagctggag gttggacaaa tgtgaacatc
       2401 gttatgaact atatccgcaa tcttgactct gaaaccgggg ccatggtgag actgctcgaa
       2461 gatggtgact acccatacga tgttccagat tacgctTAAG AATTCgatat caagcttatc
       2521 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
       2581 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
       2641 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
       2701 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
       2761 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
       2821 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
       2881 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
       2941 ctcgcctatg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
       3001 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
       3061 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
       3121 cgagcgctgc tcgagagatc tacgggtggc atccctgtga cccctcccca gtgcctctcc
       3181 tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca
       3241 tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga
       3301 gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct
       3361 ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc
       3421 tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat
       3481 ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct
       3541 aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca
       3601 ctgctccctt ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg agcggccgca
       3661 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
       3721 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
       3781 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
       3841 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
       3901 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
       3961 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
       4021 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
       4081 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
       4141 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
       4201 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
       4261 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
       4321 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
       4381 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
       4441 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
       4501 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
       4561 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
       4621 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
       4681 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
       4741 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
       4801 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
       4861 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
       4921 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
       4981 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
       5041 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
       5101 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
       5161 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
       5221 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
       5281 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
       5341 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
       5401 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
       5461 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
       5521 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
       5581 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
       5641 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
       5701 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
       5761 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
       5821 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
       5881 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
       5941 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
       6001 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
       6061 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
       6121 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
       6181 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
       6241 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
       6301 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
       6361 cggttcctgg ccttttgctg gccttttgct cacatgt
//

