LOCUS       pAAV-EF1a-EYFP-W        6067 bp    DNA     circular     20-MAY-2010
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|538328968|
COMMENT     VNTDBDATE|538763081|
COMMENT     VNTNAME|pAAV-EF1a-EYFP-WPRE|
COMMENT     VNTAUTHORNAME|William Hendriks|
COMMENT     VNTAUTHOREML|hendriks.william@mgh.harvard.edu|
COMMENT     VNTAUTHORNAME|Feng Zhang|
COMMENT     VNTAUTHOREML|zhang72@fas.harvard.edu|
FEATURES             Location/Qualifiers
     gene            1..141
                     /dnas_title="L-ITR"
                     /gene="L-ITR"
                     /vntifkey="21"
                     /label=L-ITR
     gene            3139..3617
                     /dnas_title="hGH polyA"
                     /gene="hGH polyA"
                     /vntifkey="25"
                     /label=hGH polyA
     gene            3657..3797
                     /dnas_title="R-ITR"
                     /gene="R-ITR"
                     /vntifkey="21"
                     /label=R-ITR
     exon            3889..4195
                     /dnas_title="f1 Origin"
                     /gene="f1 Origin"
                     /vntifkey="33"
                     /label=f1 Origin
     exon            4714..5571
                     /dnas_title="AmpR"
                     /gene="AmpR"
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      5722..6389
                     /dnas_title="pUC ori"
                     /gene="pUC ori"
                     /vntifkey="33"
                     /label=pUC ori
     gene            2511..3119
                     /dnas_title="WPRE"
                     /gene="WPRE"
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    588..1404
                     /note="CBh"
     CDS             1420..2466
                     /label=Cre
     CDS             2467..2493
                     /dnas_title="HA"
                     /vntifkey="4"
                     /label=HA
     promoter        156..404
                     /dnas_title="Human U6 Promoter"
                     /vntifkey="29"
                     /label=Human U6 Promoter
     misc_feature    405..504
                     /note="sgRNA backbone (SapI)"
     misc_feature    415..421
                     /note="SapI"
     misc_feature    406..412
                     /note="SapI"
ORIGIN      
          1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
         61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
        121 actccatcac taggggttcc tgcggccgca cgcgtgaggg cctatttccc atgattcctt
        181 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
        241 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
        301 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
        361 tcgatttctt ggctttatat atcttGTGGA AAGGACGAAA CACCggaaga gcgagctctt
        421 ctgttttaga gctaGAAAta gcaagttaaa ataaggctag tccgttatca acttgaaaaa
        481 gtggcaccga gtcggtgcTT TTTTgtttta gagctagaaa tagcaagtta aaataaggct
        541 agtccgtagc gcgtgcgcca attctgcaga caaattctag aggtacccgt tacataactt
        601 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
        661 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
        721 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
        781 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
        841 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
        901 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
        961 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg
       1021 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca
       1081 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
       1141 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgacgctgcc ttcgccccgt
       1201 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
       1261 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagctga gcaagaggta
       1321 agggtttaag ggatggttgg ttggtggggt attaatgttt aattacctgg agcacctgcc
       1381 tgaaatcact ttttttcagg ttGGACCGGT gccaccatgc ccaagaagaa gaggaaggtg
       1441 tccaatctcc tgactgttca ccagaacctc cctgcgctgc cagtagatgc cactagcgat
       1501 gaggtcagga aaaatctcat ggatatgttt agggatagac aggcgttttc tgaacacacc
       1561 tggaaaatgc tgcttagcgt gtgccgatcc tgggcagcct ggtgtaagct gaacaatcgc
       1621 aaatggttcc ccgccgagcc ggaggacgtg cgcgattacc tgctgtatct ccaggcaaga
       1681 gggctggctg tcaagactat ccagcagcac ttgggccaac tgaatatgct gcatcgacgc
       1741 agcgggctcc cccggcctag cgattcaaac gcagtctccc ttgttatgag gagaattaga
       1801 aaggaaaacg tagatgcggg tgagagggct aagcaggctc tcgcttttga gcggactgat
       1861 ttcgaccagg tcagatccct gatggagaac agcgatcggt gccaggacat caggaacctc
       1921 gcatttctgg gaattgcata taacacactt ctgcgcatag ctgagatcgc ccggatcaga
       1981 gtgaaagaca tcagtcgaac ggacggcggc cggatgctta ttcatattgg acgcacaaag
       2041 acattggtca gcaccgctgg cgttgaaaag gccttgtccc tgggcgtaac gaagctggtg
       2101 gaaagatgga tctcagtgtc cggcgtggct gacgacccta ataattactt gttctgtcga
       2161 gtgagaaaaa acggagtcgc cgcgccctct gccaccagcc aattgagtac acgggccctt
       2221 gaagggatct ttgaggcaac ccaccgactc atatacggag ccaaggatga cagtggccag
       2281 aggtatctcg cctggtcagg tcattctgct agggtggggg ccgcacgaga catggcgcgg
       2341 gcaggagtct ccataccaga gattatgcaa gctggaggtt ggacaaatgt gaacatcgtt
       2401 atgaactata tccgcaatct tgactctgaa accggggcca tggtgagact gctcgaagat
       2461 ggtgactacc catacgatgt tccagattac gctTAAGAAT TCgatatcaa gcttatcgat
       2521 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
       2581 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
       2641 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
       2701 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
       2761 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
       2821 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
       2881 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
       2941 gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
       3001 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
       3061 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga
       3121 gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg
       3181 ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca
       3241 ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca
       3301 aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga
       3361 gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc
       3421 tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt
       3481 tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat
       3541 ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg
       3601 ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga
       3661 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
       3721 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
       3781 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
       3841 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
       3901 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
       3961 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
       4021 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
       4081 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
       4141 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
       4201 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
       4261 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
       4321 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
       4381 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
       4441 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
       4501 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
       4561 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
       4621 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
       4681 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
       4741 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
       4801 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
       4861 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
       4921 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
       4981 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
       5041 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
       5101 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
       5161 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
       5221 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
       5281 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
       5341 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
       5401 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
       5461 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
       5521 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
       5581 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
       5641 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
       5701 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
       5761 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
       5821 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
       5881 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
       5941 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
       6001 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
       6061 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
       6121 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
       6181 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
       6241 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
       6301 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
       6361 ttcctggcct tttgctggcc ttttgctcac atgt
//

