LOCUS       pEGFP-N1-FLAGC          4777 bp    DNA     circular     17-SEP-2014
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     gene            45..764
                     /gene="egfp"
                     /vntifkey="60"
     CDS             45..764
                     /gene="egfp"
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced green fluorescent protein"
                     /protein_id="AAB02574.1"
                     /db_xref="GI:1377912"
                     /vntifkey="4"
                     /label=EGFP
     CDS             1995..2789
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /vntifkey="4"
                     /label=Kana/NeoR
     misc_feature    778..1006
                     /vntifkey="21"
                     /label=SV40\PolyA
     polyA_signal    3025..3222
                     /vntifkey="25"
                     /label=HSV\TK\PolyA
     misc_feature    6..29
                     /vntifkey="21"
                     /label=FLAGC
     misc_feature    4109..4661
                     /vntifkey="21"
                     /label=CMV\promoter
     misc_feature    3381..4000
                     /vntifkey="21"
                     /label=pBR322\Ori
     misc_feature    complement(1147..1453)
                     /vntifkey="21"
                     /label=f1\Ori
     misc_feature    1644..1912
                     /vntifkey="21"
                     /label=SV40\Promoter
BASE COUNT     1153 a      1298 c      1258 g      1068 t 
ORIGIN
        1 cgcgtgatta caaggatgac gacgataagc caccggtcgc caccatggtg agcaagggcg 
       61 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 
      121 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 
      181 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga 
      241 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca 
      301 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 
      361 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 
      421 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 
      481 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact 
      541 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga 
      601 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt 
      661 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 
      721 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta 
      781 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca 
      841 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 
      901 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 
      961 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg 
     1021 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 
     1081 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 
     1141 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 
     1201 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 
     1261 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 
     1321 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 
     1381 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 
     1441 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg 
     1501 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 
     1561 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca 
     1621 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 
     1681 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 
     1741 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 
     1801 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 
     1861 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 
     1921 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat 
     1981 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 
     2041 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 
     2101 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 
     2161 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 
     2221 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 
     2281 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 
     2341 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 
     2401 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 
     2461 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 
     2521 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 
     2581 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 
     2641 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 
     2701 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 
     2761 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 
     2821 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 
     2881 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 
     2941 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc 
     3001 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc 
     3061 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag 
     3121 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc 
     3181 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg 
     3241 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 
     3301 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 
     3361 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 
     3421 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 
     3481 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 
     3541 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 
     3601 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 
     3661 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 
     3721 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 
     3781 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 
     3841 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 
     3901 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 
     3961 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 
     4021 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 
     4081 ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata 
     4141 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 
     4201 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 
     4261 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 
     4321 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 
     4381 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 
     4441 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat 
     4501 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt 
     4561 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc 
     4621 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc 
     4681 gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttcgaa ttctgcagtc 
     4741 gacggtaccg cgggcccggg atccgccgcc accatga 
//
