LOCUS       pICE-RNaseHI-D10R-E48R-NLS-mCherry        6250 bp    DNA     circular     18-SEP-2014
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     misc_feature    2426..3028
                     /vntifkey="21"
                     /label=PuroR
     rep_origin      complement(3672..4342)
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     promoter        complement(5348..5446)
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     CDS             complement(4487..5347)
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     misc_feature    5523..6247
                     /vntifkey="21"
                     /label=CMVTET
     misc_feature    6109..6148
                     /vntifkey="21"
                     /label=TetO
     polyA_site      1336..1560
                     /invitrogen="1870000"
                     /vntifkey="26"
                     /label=BGH\pA
     rep_origin      1606..2034
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=f1\origin
     promoter        2039..2408
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     polyA_signal    3158..3288
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     misc_feature    552..1264
                     /vntifkey="21"
                     /label=mCherry
     misc_feature    513..539
                     /vntifkey="21"
                     /label=NLS
     misc_feature    12..476
                     /vntifkey="21"
                     /label=RNase\HI\D10R\E48R
BASE COUNT     1444 a      1759 c      1669 g      1378 t 
ORIGIN
        1 agcttgccac catgctgaaa caggtggaaa tcttcacccg gggcagctgt ctgggcaatc 
       61 ctggccctgg cggatacggc gccatcctga gataccgggg cagagagaaa accttcagcg 
      121 ccggctacac ccggaccacc aacaaccgga tgagactgat ggccgccatc gtggccctgg 
      181 aagccctgaa agaacactgc gaagtgatcc tgagcaccga cagccagtac gtgcggcagg 
      241 gcatcaccca gtggattcac aactggaaga agcggggctg gaaaaccgcc gacaagaaac 
      301 ccgtgaagaa cgtggacctg tggcagagac tggatgccgc cctgggacag caccagatca 
      361 agtgggagtg ggtcaagggc cacgccggcc accctgagaa tgagagatgt gacgagctgg 
      421 ccagagccgc cgctatgaac cccaccctgg aagataccgg ctaccaggtg gaagtgggca 
      481 gccccaagaa aaagcggaaa gtgggcacgc gtggccccaa aaagaaacgc aaagtgggac 
      541 cggtcgccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca 
      601 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg 
      661 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg 
      721 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct 
      781 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca 
      841 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct 
      901 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg 
      961 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc 
     1021 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc 
     1081 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg 
     1141 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg 
     1201 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt 
     1261 aaagcggccg cgaattcgga tccggtaccc tcgagacgcg tctgcagtct agataaaccc 
     1321 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg 
     1381 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 
     1441 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca 
     1501 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg 
     1561 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg 
     1621 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 
     1681 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 
     1741 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 
     1801 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 
     1861 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 
     1921 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 
     1981 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct 
     2041 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 
     2101 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 
     2161 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 
     2221 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 
     2281 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 
     2341 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 
     2401 cattttcgga tctgatcagc acgtgaccat gaccgagtac aagcccacgg tgcgcctcgc 
     2461 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 
     2521 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga 
     2581 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 
     2641 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 
     2701 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 
     2761 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 
     2821 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 
     2881 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 
     2941 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 
     3001 gtgcatgacc cgcaagcccg gtgcctgaca cgtgctacga gatttcgatt ccaccgccgc 
     3061 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 
     3121 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 
     3181 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 
     3241 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tagccgtcga 
     3301 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 
     3361 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 
     3421 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 
     3481 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
     3541 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     3601 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 
     3661 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 
     3721 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 
     3781 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 
     3841 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 
     3901 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 
     3961 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 
     4021 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 
     4081 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 
     4141 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 
     4201 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 
     4261 gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
     4321 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 
     4381 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 
     4441 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 
     4501 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 
     4561 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 
     4621 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 
     4681 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 
     4741 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 
     4801 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 
     4861 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 
     4921 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 
     4981 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 
     5041 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 
     5101 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 
     5161 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 
     5221 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 
     5281 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 
     5341 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 
     5401 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 
     5461 cccgaaaagt gccacctgac gtcgacggat cgggagatcc tgtacgggcc agatatccgc 
     5521 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 
     5581 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 
     5641 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 
     5701 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 
     5761 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 
     5821 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 
     5881 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat 
     5941 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt 
     6001 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc 
     6061 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg 
     6121 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga 
     6181 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca 
     6241 gcctccggca 
//
