LOCUS       pLenti-CaMKIIa-A       10843 bp    DNA     circular     17-SEP-2014
SOURCE
  ORGANISM
KEYWORDS
COMMENT     This file is created by Vector NTI











            http://www.invitrogen.com/











            VNTDATE|617806315|











            VNTDBDATE|617806315|











            LSOWNER|











            VNTNAME|FCK (1.3)-eArch 3.0-EYFP-W|











            VNTAUTHORNAME|Charu Ramakrishnan|











            VNTOAUTHORNAME|Feng Zhang|
FEATURES             Location/Qualifiers
     LTR             6648..6828
                     /label="3' LTR"
     misc_feature    5525..6133
                     /label="WPRE"
     LTR             835..1015
                     /label="5'LTR"
     misc_binding    1691..1895
                     /label="RRE"
     promoter        2598..3890
                     /label="a-CaMKII(1.3)"
     enhancer        4713..4772
                     /label="TS"
     enhancer        5487..5507
                     /label="ER export"
     misc_feature    4773..5486
                     /label="eGFP"
     misc_feature    3906..4679
                     /label="Archer1"
     misc_feature    complement(9850..10710)
                     /label="AmpicillinR"
     enhancer        318..605
                     /label="CAG enhancer"
     promoter        239..815
                     /label="CMV Promoter"
     promoter        complement(10752..10780)
                     /label="AmpR Promoter"
     misc_binding    5511..5516
                     /label="EcoRI"
     misc_binding    3894..3899
                     /label="BamHI"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 
     2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt 
     2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag 
     2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat 
     2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca 
     2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg 
     2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga 
     2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg 
     3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga 
     3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac 
     3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg 
     3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa 
     3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt 
     3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga 
     3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc 
     3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa 
     3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag 
     3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac 
     3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag 
     3661 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac 
     3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg 
     3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt 
     3841 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatccg 
     3901 ccaccatgga ccccatcgct ctgcaggctg gttacgacct gctgggtgac ggcagacctg 
     3961 aaactctgtg gctgggcatc ggcactctgc tgatgctgat tggaaccttc tactttctgg 
     4021 tccgcggatg gggagtcacc gataaggatg cccgggaata ttacgctgtg actatcctgg 
     4081 tgcccggaat cgcatccgcc gcatatctgt ctatgttctt tggtatcggg cttactgagg 
     4141 tgaccgtcgg gggcgaaatg ttggatatct attatgccag gtacgccgag tggctgtttt 
     4201 gcaccccact tctgctgctg gatctggccc ttctcgctaa ggtggatcgg gtgaccatcg 
     4261 gcaccctggt gggtgtggac gccctgatga tcgtcactgg cctcatcgga gccttgagcc 
     4321 acacggccat agccagatac agttggtggt tgttctctac aatttgcatg atagtggtgc 
     4381 tctattttct ggctacatcc ctgcgatctg ctgcaaagga gcggggcccc gaggtggcat 
     4441 ctacctttaa caccctgaca gctctggtct tggtgctgtg gaccgcttac cctatcctgt 
     4501 ggatcatagg cactgagggc gctggcgtgg tgggcctggg catcgaaact ctgctgttta 
     4561 tggtgttgga cgtgactgcc aaggtcggct ttggctttat cctgttgaga tcccgggcta 
     4621 ttctgggcga caccgaggca ccagaaccca gtgccggtgc cgatgtcagt gccgccgacc 
     4681 gaccggtagt agcagtgagc aaggcggccg ccaagagcag gatcaccagc gagggcgagt 
     4741 acatccccct ggaccagatc gacatcaacg tggtgagcaa gggcgaggag ctgttcaccg 
     4801 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 
     4861 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atttgcacca 
     4921 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt 
     4981 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg 
     5041 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 
     5101 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 
     5161 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 
     5221 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 
     5281 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 
     5341 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag 
     5401 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 
     5461 ctctcggcat ggacgagctg tacaagttct gctacgagaa cgaggtgtaa gaattcgata 
     5521 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 
     5581 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 
     5641 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 
     5701 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 
     5761 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 
     5821 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 
     5881 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct 
     5941 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 
     6001 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 
     6061 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 
     6121 cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata 
     6181 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt 
     6241 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta 
     6301 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag 
     6361 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca 
     6421 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag 
     6481 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg 
     6541 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc 
     6601 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt 
     6661 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 
     6721 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 
     6781 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa 
     6841 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc 
     6901 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag 
     6961 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag 
     7021 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct 
     7081 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt 
     7141 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 
     7201 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 
     7261 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 
     7321 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 
     7381 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 
     7441 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 
     7501 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa 
     7561 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 
     7621 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 
     7681 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 
     7741 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 
     7801 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga 
     7861 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta 
     7921 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg 
     7981 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc 
     8041 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt 
     8101 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt 
     8161 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg 
     8221 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc 
     8281 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg 
     8341 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg 
     8401 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 
     8461 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa 
     8521 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 
     8581 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 
     8641 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt 
     8701 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 
     8761 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 
     8821 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 
     8881 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 
     8941 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 
     9001 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 
     9061 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 
     9121 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 
     9181 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 
     9241 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 
     9301 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 
     9361 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 
     9421 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 
     9481 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 
     9541 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 
     9601 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 
     9661 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 
     9721 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 
     9781 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 
     9841 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 
     9901 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 
     9961 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 
    10021 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 
    10081 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 
    10141 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 
    10201 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 
    10261 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 
    10321 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 
    10381 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 
    10441 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 
    10501 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 
    10561 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 
    10621 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 
    10681 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 
    10741 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 
    10801 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac 
//