LOCUS Exported File 6625 bp ds-DNA circular SYN 29-SEP-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS pSBbi_RP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6625) AUTHORS Eric Kowarz TITLE . JOURNAL . FEATURES Location/Qualifiers source 1..6625 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 8..11 /note="tata" /note="color: #cc0000" misc_feature 8..11 /note="tata" /note="color: #cc0000" misc_feature 12..236 /note="5'-ITR" /note="Sleeping Beauty inverted tandem repeat" /note="color: #cc00cc" misc_feature 242..283 /note="5'-proxITR" /note="modified region proximal to 5'-ITR - contains CTCF binding sites" /note="color: #cc0000" polyA_signal complement(294..591) /note="bGH-pA" /note="bovine growth hormone polyadenylation signal" /note="color: #cc0000; direction: LEFT" misc_feature 592..604 /note="3'-SfiI" /note="GGCCTGTCAGGCC" /note="color: #a6acb3" misc_feature 605..800 /note="filler DNA" /note="color: #cc0000; direction: RIGHT" misc_feature 801..813 /note="5'-SfiI" /note="GGCCTCTGAGGCC" /note="color: #a6acb3" promoter complement(819..2011) /note="EF1a promoter" /note="constitutive promoter" /note="color: #cc0000; direction: LEFT" exon complement(819..837) /note="EF1a Exon2" /note="color: #cc0000; direction: LEFT" intron complement(838..1780) /note="EF1a Intron1" /note="color: #cc0000; direction: LEFT" promoter 2018..2629 /note="RPBSA promoter" /note="constitutive promoter" /note="color: #cc0000; direction: RIGHT" promoter 2018..2629 /note="RPBSA" /note="constitutive promoter" /note="color: #cc0000; direction: RIGHT" promoter 2018..2452 /note="RPL13a Promoter" /note="color: #cc0000; direction: RIGHT" exon 2452..2504 /note="RPL41 exon1" /note="color: #cc0000; direction: RIGHT" intron 2505..2619 /note="RPL41 intron1" /note="color: #cc0000; direction: RIGHT" exon 2620..2629 /note="RPL41 exon2" /note="color: #cc0000; direction: RIGHT" CDS 2643..3344 /codon_start=1 /product="dimeric variant of DsRed fluorescent protein" /note="dTomato" /note="mammalian codon-optimized" /note="color: #ff0000" /translation="MLSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLYGMDELYK" CDS 3345..3410 /codon_start=1 /note="P2A" /note="color: #cc0000" /translation="GSGATNFSLLKQAGDVEENPGP" CDS 3411..4010 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 4028..4328 /note="rBG-pA" /note="rabbit beta-globin polyadenylation signal" /note="color: #cc0000; direction: RIGHT" misc_feature 4339..4380 /note="3'-proxITR" /note="modified region proximal to 3'-ITR - contains CTCF binding sites" /note="color: #cc0000" misc_feature 4381..4605 /note="3'-ITR" /note="Sleeping Beauty inverted tandem repeat" /note="color: #cc00cc" misc_feature 4606..4609 /note="tata" /note="color: #cc0000" rep_origin complement(4793..5381) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(5552..6412) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments:    1: 5552 .. 6343 / #ccffcc    2: 6344 .. 6412 / #ccffcc / signal sequence" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(6413..6517) /gene="bla" /note="AmpR promoter" /note="color: #ffffff; direction: LEFT" ORIGIN 1 ggatccctat acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact 61 cgtttttcaa ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta 121 ggacatctac tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta 181 tttcacttat aattcactgt atcacaattc cagtgggtca gaagtttaca tacactaagt 241 tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg tccgctatct agtaggccca 301 gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt 361 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca 421 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc 481 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga 541 aggcacagtc gaggctgatc agcgagctct agagaattga tccccaagct tggcctgaca 601 ggccttcgaa ttagagagaa ggaaagacgc aaccaggaag cccaccaaga gaaagaggca 661 tttcctgaaa agattcccct ttttggagag ccctacaagg tatttactga acactagaca 721 ttgaagtcct gatttatcac aatgttgaac cctatgatga aacaattcag tataattgag 781 ttcgaaactc gagccatggt ggcctcagag gccagcttgg ggtagttttc acgacacctt 841 aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc aagatccaaa 901 ctcaaaaagg gcaaattcca aggagaatta catcaagtgc caagctggcc taacttcagt 961 ctccacccac tcagtatggg gaaactccat cgcataaaac ccctcccccc aacctaaaga 1021 cgacgtactc caaaagctcg agaactaatc gaggtgcctg gacggcgccc ggtactccgt 1081 ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg tgggtgactc 1141 acccgcccgc tctcccgagc gccgcgtcct ccattttgag ctccctgcag cagggccggg 1201 aagcggccat ctttccgctc acgcaactgg tgccgaccgg gccagccttg ccgcccaggg 1261 cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag cttgagacta 1321 cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac atgtgcgctg 1381 ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag tgtgcagatc 1441 ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag gtcatcaaaa 1501 attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt gccaccagat 1561 tcgcacgcgg cggccccagc gcccaggcca agcctcaact caagcacgag gcgaaggggc 1621 tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct cgggatcaag 1681 aatcacgtac tgcagccagg ggcgtggaag taattcaagg cacgcaaggg ccataacccg 1741 taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg gcggcaaacc 1801 cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct 1861 cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc 1921 tctaggcacc ggttcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg 1981 tgcgctctgc ccactgacgg gcaccggagc ctctaggcga gaccctgtct cacaaaataa 2041 agtaagcccg gactgagtgc ggaaaggcgg gcctggcggg tctggtctcc ccatgcgggc 2101 caccagaggc cctgcagcct tcagtcgctt gaaggggtaa tggcgcttcc actcacaaac 2161 atggcggaca gagcgtgtga acgagatgaa cagcccctca aaaatatggc cgccgaggct 2221 ggacggccgt gccccagcag caccgcctcc gcgccccacg tgatctctcg ccgggcacag 2281 cgctgaccgc ggaggtccaa ccggaagaat gtccggattg gacattcgga agagggcccg 2341 ccttccctgg ggaatctctg cgcacgcgca gaacgcttcg accaatgaaa acacaggaag 2401 ccgtccgcgc aaccgcgttg cgtcacttct gccgcccctg tttcaaggta tatagccgta 2461 gacggaactt cgcctttctc tcggccttag cgccattttt ttgggtgagt gttttttggt 2521 tcctgcgttg ggattccgtg tacaatccat agacatctga cctcggcact tagcatcatc 2581 acagcaaact aactgtagcc tttctctctt tccctgtaga aacctctgca cctgaggcca 2641 ccatgttgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg 2701 agggctccat gaacggccat gagttcgaga tcgagggcga gggcgagggc cgcccctacg 2761 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg 2821 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca 2881 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact 2941 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga 3001 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga 3061 agacaatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg 3121 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga 3181 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc 3241 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg 3301 gccgccacca cctgttcctg tacggcatgg acgagctgta caagggcagt ggagctacta 3361 acttcagcct gctgaagcag gctggtgacg tcgaggagaa tcctggcccc atgaccgagt 3421 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg 3481 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg 3541 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 3601 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 3661 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 3721 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 3781 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 3841 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 3901 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 3961 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga ttcgaaggcc 4021 tgtcgtgaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc 4081 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac 4141 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca 4201 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat 4261 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccat atgctggctg 4321 ccatgaacta gctactcggg accccttacc gaaacatcgc cgcattctgc agaggagtcg 4381 agtgtatgta aacttctgac ccactgggaa tgtgatgaaa gaaataaaag ctgaaatgaa 4441 tcattctctc tactattatt ctgatatttc acattcttaa aataaagtgg tgatcctaac 4501 tgacctaaga cagggaattt ttactaggat taaatgtcag gaattgtgaa aaagtgagtt 4561 taaatgtatt tggctaaggt gtatgtaaac ttccgacttc aactgtatag ggatccgctt 4621 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 4681 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 4741 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 4801 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 4861 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 4921 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 4981 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 5041 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 5101 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 5161 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 5221 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 5281 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 5341 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 5401 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 5461 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 5521 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 5581 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 5641 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 5701 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 5761 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 5821 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 5881 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 5941 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 6001 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 6061 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 6121 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 6181 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 6241 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 6301 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 6361 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 6421 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 6481 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 6541 ctgatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg 6601 taagcgttaa tattttgtta aaatt //