LOCUS Exported File 7051 bp ds-DNA circular SYN 29-SEP-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS pSBbi_RH SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7051) AUTHORS Eric Kowarz TITLE . JOURNAL . FEATURES Location/Qualifiers source 1..7051 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 8..11 /note="tata" /note="color: #cc0000" misc_feature 8..11 /note="tata" /note="color: #cc0000" misc_feature 12..236 /note="5'-ITR" /note="Sleeping Beauty inverted tandem repeat" /note="color: #cc00cc" misc_feature 242..283 /note="5'-proxITR" /note="modified region proximal to 5'-ITR - contains CTCF binding sites" /note="color: #cc0000" polyA_signal complement(294..591) /note="bGH-pA" /note="bovine growth hormone polyadenylation signal" /note="color: #cc0000; direction: LEFT" misc_feature 592..604 /note="3'-SfiI" /note="GGCCTGTCAGGCC" /note="color: #a6acb3" misc_feature 605..800 /note="filler DNA" /note="color: #cc0000; direction: RIGHT" misc_feature 801..813 /note="5'-SfiI" /note="GGCCTCTGAGGCC" /note="color: #a6acb3" promoter complement(819..2011) /note="EF1a promoter" /note="constitutive promoter" /note="color: #cc0000; direction: LEFT" exon complement(819..837) /note="EF1a Exon2" /note="color: #cc0000; direction: LEFT" intron complement(838..1780) /note="EF1a Intron1" /note="color: #cc0000; direction: LEFT" promoter 2018..2629 /note="RPBSA promoter" /note="constitutive promoter" /note="color: #cc0000; direction: RIGHT" promoter 2018..2629 /note="RPBSA" /note="constitutive promoter" /note="color: #cc0000; direction: RIGHT" promoter 2018..2452 /note="RPL13a Promoter" /note="color: #cc0000; direction: RIGHT" exon 2452..2504 /note="RPL41 exon1" /note="color: #cc0000; direction: RIGHT" intron 2505..2619 /note="RPL41 intron1" /note="color: #cc0000; direction: RIGHT" exon 2620..2629 /note="RPL41 exon2" /note="color: #cc0000; direction: RIGHT" CDS 2643..3344 /codon_start=1 /product="dimeric variant of DsRed fluorescent protein" /note="dTomato" /note="mammalian codon-optimized" /note="color: #ff0000" /translation="MLSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLYGMDELYK" CDS 3345..3410 /codon_start=1 /note="P2A" /note="color: #cc0000" /translation="GSGATNFSLLKQAGDVEENPGP" CDS 3411..4436 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /note="HygR" /note="confers resistance to hygromycin" /note="color: #ccffcc" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" misc_feature 4454..4754 /note="rBG-pA" /note="rabbit beta-globin polyadenylation signal" /note="color: #cc0000; direction: RIGHT" misc_feature 4765..4806 /note="3'-proxITR" /note="modified region proximal to 3'-ITR - contains CTCF binding sites" /note="color: #cc0000" misc_feature 4807..5031 /note="3'-ITR" /note="Sleeping Beauty inverted tandem repeat" /note="color: #cc00cc" misc_feature 5032..5035 /note="tata" /note="color: #cc0000" rep_origin complement(5219..5807) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(5978..6838) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments:    1: 5978 .. 6769 / #ccffcc    2: 6770 .. 6838 / #ccffcc / signal sequence" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(6839..6943) /gene="bla" /note="AmpR promoter" /note="color: #ffffff; direction: LEFT" ORIGIN 1 ggatccctat acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact 61 cgtttttcaa ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta 121 ggacatctac tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta 181 tttcacttat aattcactgt atcacaattc cagtgggtca gaagtttaca tacactaagt 241 tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg tccgctatct agtaggccca 301 gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt 361 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca 421 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc 481 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga 541 aggcacagtc gaggctgatc agcgagctct agagaattga tccccaagct tggcctgaca 601 ggccttcgaa ttagagagaa ggaaagacgc aaccaggaag cccaccaaga gaaagaggca 661 tttcctgaaa agattcccct ttttggagag ccctacaagg tatttactga acactagaca 721 ttgaagtcct gatttatcac aatgttgaac cctatgatga aacaattcag tataattgag 781 ttcgaaactc gagccatggt ggcctcagag gccagcttgg ggtagttttc acgacacctt 841 aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc aagatccaaa 901 ctcaaaaagg gcaaattcca aggagaatta catcaagtgc caagctggcc taacttcagt 961 ctccacccac tcagtatggg gaaactccat cgcataaaac ccctcccccc aacctaaaga 1021 cgacgtactc caaaagctcg agaactaatc gaggtgcctg gacggcgccc ggtactccgt 1081 ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg tgggtgactc 1141 acccgcccgc tctcccgagc gccgcgtcct ccattttgag ctccctgcag cagggccggg 1201 aagcggccat ctttccgctc acgcaactgg tgccgaccgg gccagccttg ccgcccaggg 1261 cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag cttgagacta 1321 cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac atgtgcgctg 1381 ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag tgtgcagatc 1441 ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag gtcatcaaaa 1501 attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt gccaccagat 1561 tcgcacgcgg cggccccagc gcccaggcca agcctcaact caagcacgag gcgaaggggc 1621 tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct cgggatcaag 1681 aatcacgtac tgcagccagg ggcgtggaag taattcaagg cacgcaaggg ccataacccg 1741 taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg gcggcaaacc 1801 cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct 1861 cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc 1921 tctaggcacc ggttcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg 1981 tgcgctctgc ccactgacgg gcaccggagc ctctaggcga gaccctgtct cacaaaataa 2041 agtaagcccg gactgagtgc ggaaaggcgg gcctggcggg tctggtctcc ccatgcgggc 2101 caccagaggc cctgcagcct tcagtcgctt gaaggggtaa tggcgcttcc actcacaaac 2161 atggcggaca gagcgtgtga acgagatgaa cagcccctca aaaatatggc cgccgaggct 2221 ggacggccgt gccccagcag caccgcctcc gcgccccacg tgatctctcg ccgggcacag 2281 cgctgaccgc ggaggtccaa ccggaagaat gtccggattg gacattcgga agagggcccg 2341 ccttccctgg ggaatctctg cgcacgcgca gaacgcttcg accaatgaaa acacaggaag 2401 ccgtccgcgc aaccgcgttg cgtcacttct gccgcccctg tttcaaggta tatagccgta 2461 gacggaactt cgcctttctc tcggccttag cgccattttt ttgggtgagt gttttttggt 2521 tcctgcgttg ggattccgtg tacaatccat agacatctga cctcggcact tagcatcatc 2581 acagcaaact aactgtagcc tttctctctt tccctgtaga aacctctgca cctgaggcca 2641 ccatgttgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg 2701 agggctccat gaacggccat gagttcgaga tcgagggcga gggcgagggc cgcccctacg 2761 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg 2821 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca 2881 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact 2941 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga 3001 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga 3061 agacaatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg 3121 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga 3181 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc 3241 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg 3301 gccgccacca cctgttcctg tacggcatgg acgagctgta caagggcagt ggagctacta 3361 acttcagcct gctgaagcag gctggtgacg tcgaggagaa tcctggcccc atgaaaaagc 3421 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg 3481 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc 3541 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt 3601 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca 3661 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc 3721 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg 3781 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat 3841 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa 3901 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt 3961 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg 4021 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg 4081 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc 4141 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg 4201 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg 4261 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg 4321 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag 4381 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagttcg 4441 aaggcctgtc gtgaattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt 4501 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat 4561 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc 4621 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca 4681 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgccatatgc 4741 tggctgccat gaactagcta ctcgggaccc cttaccgaaa catcgccgca ttctgcagag 4801 gagtcgagtg tatgtaaact tctgacccac tgggaatgtg atgaaagaaa taaaagctga 4861 aatgaatcat tctctctact attattctga tatttcacat tcttaaaata aagtggtgat 4921 cctaactgac ctaagacagg gaatttttac taggattaaa tgtcaggaat tgtgaaaaag 4981 tgagtttaaa tgtatttggc taaggtgtat gtaaacttcc gacttcaact gtatagggat 5041 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 5101 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 5161 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 5221 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 5281 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 5341 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 5401 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 5461 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 5521 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 5581 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 5641 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 5701 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 5761 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 5821 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 5881 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 5941 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 6001 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 6061 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 6121 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 6181 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 6241 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 6301 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 6361 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 6421 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 6481 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 6541 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 6601 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 6661 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 6721 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 6781 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 6841 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 6901 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 6961 tgccacctga tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 7021 aaattgtaag cgttaatatt ttgttaaaat t //