LOCUS Exported File 6817 bp ds-DNA circular SYN 29-SEP-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS pSBbi_BN SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6817) AUTHORS Eric Kowarz TITLE . JOURNAL . FEATURES Location/Qualifiers source 1..6817 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 8..11 /note="tata" /note="color: #cc0000" misc_feature 8..11 /note="tata" /note="color: #cc0000" misc_feature 12..236 /note="5'-ITR" /note="Sleeping Beauty inverted tandem repeat" /note="color: #cc00cc" misc_feature 242..283 /note="5'-proxITR" /note="modified region proximal to 5'-ITR - contains CTCF binding sites" /note="color: #cc0000" polyA_signal complement(294..591) /note="bGH-pA" /note="bovine growth hormone polyadenylation signal" /note="color: #cc0000; direction: LEFT" misc_feature 592..604 /note="3'-SfiI" /note="GGCCTGTCAGGCC" /note="color: #a6acb3" misc_feature 605..800 /note="filler DNA" /note="color: #cc0000; direction: RIGHT" misc_feature 801..813 /note="5'-SfiI" /note="GGCCTCTGAGGCC" /note="color: #a6acb3" promoter complement(819..2011) /note="EF1a promoter" /note="constitutive promoter" /note="color: #cc0000; direction: LEFT" exon complement(819..837) /note="EF1a Exon2" /note="color: #cc0000; direction: LEFT" intron complement(838..1780) /note="EF1a Intron1" /note="color: #cc0000; direction: LEFT" promoter 2018..2629 /note="RPBSA" /note="constitutive promoter" /note="color: #cc0000; direction: RIGHT" promoter 2018..2629 /note="RPBSA promoter" /note="constitutive promoter" /note="color: #cc0000; direction: RIGHT" promoter 2018..2452 /note="RPL13a Promoter" /note="color: #cc0000; direction: RIGHT" exon 2452..2504 /note="RPL41 exon1" /note="color: #cc0000; direction: RIGHT" intron 2505..2619 /note="RPL41 intron1" /note="color: #cc0000; direction: RIGHT" exon 2620..2629 /note="RPL41 exon2" /note="color: #cc0000; direction: RIGHT" CDS 2643..3341 /codon_start=1 /note="mTag-BFP" /note="color: #cc0000" /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC DLPSKLGHKLN" CDS 3342..3407 /codon_start=1 /note="P2A" /note="color: #cc0000" /translation="GSGATNFSLLKQAGDVEENPGP" CDS 3408..4202 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" misc_feature 4220..4520 /note="rBG-pA" /note="rabbit beta-globin polyadenylation signal" /note="color: #cc0000; direction: RIGHT" misc_feature 4531..4572 /note="3'-proxITR" /note="modified region proximal to 3'-ITR - contains CTCF binding sites" /note="color: #cc0000" misc_feature 4573..4797 /note="3'-ITR" /note="Sleeping Beauty inverted tandem repeat" /note="color: #cc00cc" misc_feature 4798..4801 /note="tata" /note="color: #cc0000" rep_origin complement(4985..5573) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(5744..6604) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments:    1: 5744 .. 6535 / #ccffcc    2: 6536 .. 6604 / #ccffcc / signal sequence" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(6605..6709) /gene="bla" /note="AmpR promoter" /note="color: #ffffff; direction: LEFT" ORIGIN 1 ggatccctat acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact 61 cgtttttcaa ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta 121 ggacatctac tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta 181 tttcacttat aattcactgt atcacaattc cagtgggtca gaagtttaca tacactaagt 241 tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg tccgctatct agtaggccca 301 gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt 361 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca 421 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc 481 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga 541 aggcacagtc gaggctgatc agcgagctct agagaattga tccccaagct tggcctgaca 601 ggccttcgaa ttagagagaa ggaaagacgc aaccaggaag cccaccaaga gaaagaggca 661 tttcctgaaa agattcccct ttttggagag ccctacaagg tatttactga acactagaca 721 ttgaagtcct gatttatcac aatgttgaac cctatgatga aacaattcag tataattgag 781 ttcgaaactc gagccatggt ggcctcagag gccagcttgg ggtagttttc acgacacctt 841 aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc aagatccaaa 901 ctcaaaaagg gcaaattcca aggagaatta catcaagtgc caagctggcc taacttcagt 961 ctccacccac tcagtatggg gaaactccat cgcataaaac ccctcccccc aacctaaaga 1021 cgacgtactc caaaagctcg agaactaatc gaggtgcctg gacggcgccc ggtactccgt 1081 ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg tgggtgactc 1141 acccgcccgc tctcccgagc gccgcgtcct ccattttgag ctccctgcag cagggccggg 1201 aagcggccat ctttccgctc acgcaactgg tgccgaccgg gccagccttg ccgcccaggg 1261 cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag cttgagacta 1321 cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac atgtgcgctg 1381 ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag tgtgcagatc 1441 ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag gtcatcaaaa 1501 attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt gccaccagat 1561 tcgcacgcgg cggccccagc gcccaggcca agcctcaact caagcacgag gcgaaggggc 1621 tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct cgggatcaag 1681 aatcacgtac tgcagccagg ggcgtggaag taattcaagg cacgcaaggg ccataacccg 1741 taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg gcggcaaacc 1801 cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct 1861 cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc 1921 tctaggcacc ggttcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg 1981 tgcgctctgc ccactgacgg gcaccggagc ctctaggcga gaccctgtct cacaaaataa 2041 agtaagcccg gactgagtgc ggaaaggcgg gcctggcggg tctggtctcc ccatgcgggc 2101 caccagaggc cctgcagcct tcagtcgctt gaaggggtaa tggcgcttcc actcacaaac 2161 atggcggaca gagcgtgtga acgagatgaa cagcccctca aaaatatggc cgccgaggct 2221 ggacggccgt gccccagcag caccgcctcc gcgccccacg tgatctctcg ccgggcacag 2281 cgctgaccgc ggaggtccaa ccggaagaat gtccggattg gacattcgga agagggcccg 2341 ccttccctgg ggaatctctg cgcacgcgca gaacgcttcg accaatgaaa acacaggaag 2401 ccgtccgcgc aaccgcgttg cgtcacttct gccgcccctg tttcaaggta tatagccgta 2461 gacggaactt cgcctttctc tcggccttag cgccattttt ttgggtgagt gttttttggt 2521 tcctgcgttg ggattccgtg tacaatccat agacatctga cctcggcact tagcatcatc 2581 acagcaaact aactgtagcc tttctctctt tccctgtaga aacctctgca cctgaggcca 2641 ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg 2701 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga 2761 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta 2821 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct 2881 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg 2941 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca 3001 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct 3061 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca 3121 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat 3181 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg 3241 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca 3301 gatactgcga cctccctagc aaactggggc acaagctgaa cggcagtgga gctactaact 3361 tcagcctgct gaagcaggct ggtgacgtcg aggagaatcc tggccccatg attgaacaag 3421 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 3481 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 3541 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag 3601 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 3661 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 3721 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 3781 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 3841 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 3901 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg 3961 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 4021 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 4081 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 4141 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 4201 gattcgaagg cctgtcgtga attcactcct caggtgcagg ctgcctatca gaaggtggtg 4261 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa 4321 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt 4381 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg 4441 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc 4501 atatgctggc tgccatgaac tagctactcg ggacccctta ccgaaacatc gccgcattct 4561 gcagaggagt cgagtgtatg taaacttctg acccactggg aatgtgatga aagaaataaa 4621 agctgaaatg aatcattctc tctactatta ttctgatatt tcacattctt aaaataaagt 4681 ggtgatccta actgacctaa gacagggaat ttttactagg attaaatgtc aggaattgtg 4741 aaaaagtgag tttaaatgta tttggctaag gtgtatgtaa acttccgact tcaactgtat 4801 agggatccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 4861 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 4921 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 4981 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 5041 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 5101 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 5161 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 5221 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 5281 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 5341 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 5401 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 5461 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 5521 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 5581 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 5641 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 5701 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 5761 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 5821 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 5881 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 5941 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 6001 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 6061 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 6121 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 6181 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 6241 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 6301 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 6361 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 6421 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 6481 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 6541 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 6601 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 6661 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 6721 gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 6781 atcaggaaat tgtaagcgtt aatattttgt taaaatt //