LOCUS       pRec07_08               7365 bp ds-DNA     circular     15-SEP-2014
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     New DNA(1) from 1 to 5890
COMMENT     New DNA from 1 to 5890
COMMENT     pRec07_ColE_Int7_optE6 from 1 to 5950
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2033..2716
                     /label=ColE1
                     /ApEinfo_fwdcolor=#4049ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             4498..5886
                     /label=Int7
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             5912..7297
                     /label=Int8
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    190..1630
                     /label=KanR
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2717..2859
                     /label=spacer for Kan ColE1 frag
                     /ApEinfo_fwdcolor=#ebb8ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        4388..4402
                     /vntifkey="30"
                     /label=Pbad
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#00ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3258..4136)
                     /vntifkey="4"
                     /label=AraC
                     /ApEinfo_fwdcolor=#ff7a00
                     /ApEinfo_revcolor=#ffb16a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3056..3257)
                     /label=Spacer downstream of AraC
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=#00bbbb
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4337..4467
                     /label=Pbad BBa_10500
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7298..7338
                     /label=Term rrn
                     /ApEinfo_fwdcolor=#5c6bcf
                     /ApEinfo_revcolor=#5e85df
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 aactCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA
       61 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA
      121 TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA
      181 ATTTAACGCG AATTTTAACA AAATTTCACT GACACCCTCA TCAGTGCCAA CATAGTAAGC
      241 CAGTATACAC TCCGCTAGCG CTGAGGTCTG CCTCGTGAAG AAGGTGTTGC TGACTCATAC
      301 CAGGCCTGAA TCGCCCCATC ATCCAGCCAG AAAGTGAGGG AGCCACGGTT GATGAGAGCT
      361 TTGTTGTAGG TGGACCAGTT GGTGATTTTG AACTTTTGCT TTGCCACGGA ACGGTCTGCG
      421 TTGTCGGGAA GATGCGTGAT CTGATCCTTC AACTCAGCAA AAGTTCGATT TATTCAACAA
      481 AGCCACGTTG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAA TATATCATCA
      541 TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT
      601 CAACGGGAAA CGTCTTGCTC GAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT
      661 GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA TCGATTGTAT
      721 GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT
      781 GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC
      841 AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGGAAA
      901 ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG
      961 GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT
     1021 CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT
     1081 GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAG
     1141 CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT
     1201 ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC
     1261 CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG
     1321 AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT
     1381 TTGATGCTCG ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA CTGGCAGAGC
     1441 ATTACGCTGA CTTGACGGGA CTCGAGATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC
     1501 GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCCTGCATA ACGCGAAGTA
     1561 ATCTTTTCGG TTTTAAAGAA AAAGGGCAGG GTGGTGACAC CTTGCCCGTT TTTTTGCCGG
     1621 ACTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT
     1681 TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC
     1741 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT
     1801 CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC
     1861 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC
     1921 TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT
     1981 TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG
     2041 ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC
     2101 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA
     2161 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG
     2221 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA
     2281 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA
     2341 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG
     2401 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG
     2461 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG
     2521 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG
     2581 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC
     2641 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA
     2701 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG
     2761 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT
     2821 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGg tcggcgatat aggcgccagc
     2881 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatctgctc
     2941 atgtttgaca gcttatcatc gatgcataat gtgcctgtca aatggacgaa gcagggattc
     3001 tgcaaaccct atgctactcc ctcgagccgt caattgtctg attcgttacc aattatacta
     3061 gctccggcaa aaaaacgggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa
     3121 gattacttcg cgtttgccac ctgacgtcta agaaaaggaa tattcagcaa tttgcccgtg
     3181 ccgaagaaag gcccacccgt gaaggtgagc cagtgagttg attgctacgt aattagttag
     3241 ttagccctta gtgactctta tgacaacttg acggctacat cattcacttt ttcttcacaa
     3301 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag
     3361 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc
     3421 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct
     3481 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg
     3541 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt
     3601 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac
     3661 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg
     3721 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag
     3781 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga
     3841 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga
     3901 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga
     3961 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc
     4021 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc
     4081 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt
     4141 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt
     4201 cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc
     4261 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat
     4321 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca
     4381 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt
     4441 tctccatacc cgtttttttg ggctagcAGT AATTTCAACA AAATAACTAG GATTCGAATG
     4501 AAAGTGGCCA TTTATGTTCG TGTTAGCACC GATGAACAGG CCAAAGAAGG TTTTAGCATT
     4561 CCGGCACAGC GTGAACGTCT GCGTGCATTT TGTGCAAGCC AGGGTTGGGA AATTGTGCAA
     4621 GAATATATTG AAGAAGGTTG GAGCGCAAAA GATCTGGATC GTCCGCAGAT GCAGCGTCTG
     4681 CTGAAAGATA TCAAAAAAGG CAACATTGAT ATTGTGCTGG TGTATCGTCT GGATCGCCTG
     4741 ACCCGTAGCG TTCTGGATCT GTATCTGCTG CTGCAGACCT TTGAAAAATA CAATGTGGCA
     4801 TTTCGTAGCG CCACCGAAGT TTATGATACC AGCACCGCAA TGGGTCGTCT GTTTATTACC
     4861 CTGGTTGCAG CACTGGCACA GTGGGAACGT GAAAATCTGG CAGAACGTGT TAAATTTGGT
     4921 ATCGAGCAGA TGATCGATGA AGGTAAAAAA CCGGGTGGTC ATAGCCCGTA TGGTTACAAA
     4981 TTTGATAAAG ACTTCAATTG CACCATTATT GAGGAAGAAG CAGACGTTGT TCGTATGATC
     5041 TATCGCATGT ATTGTGATGG TTATGGCTAT CGTAGCATTG CAGATCGTCT GAATGAACTG
     5101 ATGGTTAAAC CGCGTATTGC CAAAGAATGG AATCATAATA GCGTGCGTGA TATCCTGACC
     5161 AACGATATCT ATATTGGCAC CTATCGTTGG GGTGATAAAG TTGTTCCGAA TAATCATCCG
     5221 CCTATTATTA GCGAAACCCT GTTCAAAAAA GCCCAGAAAG AAAAAGAAAA ACGTGGCGTT
     5281 GATCGTAAAC GCGTTGGTAA ATTTCTGTTT ACCGGTCTGC TGCAGTGTGG TAATTGTGGT
     5341 GGCCATAAAA TGCAGGGCCA TTTTGATAAA CGTGAGCAGA AAACCTATTA CCGTTGTACC
     5401 AAATGTCACC GCATTACCAA CGAAAAAAAC ATTCTGGAAC CGCTGCTGGA TGAAATTCAG
     5461 CTGCTGATTA CCAGCAAAGA ATACTTTATG AGCAAATTCA GCGACCGCTA TGATCAGCAA
     5521 GAGGTTGTTG ATGTTAGCGC ACTGACAAAA GAACTGGAAA AAATCAAACG CCAGAAAGAG
     5581 AAATGGTACG ATCTGTATAT GGATGATCGT AACCCGATTC CGAAAGAAGA ACTGTTTGCC
     5641 AAAATTAACG AACTGAACAA AAAAGAAGAA GAAATCTATA GCAAGCTGAG CGAAGTGGAA
     5701 GAAGATAAAG AACCGGTTGA AGAGAAATAT AACCGCCTGA GCAAAATGAT CGATTTTAAA
     5761 CAGCAGTTTG AGCAGGCCAA CGACTTTACC AAAAAAGAGC TGCTGTTCAG CATCTTCGAA
     5821 AAGATTGTGA TTTATCGCGA GAAAGGCAAG CTGAAAAAAA TCACCCTGGA TTACACCCTG
     5881 AAATAAgaat AAAACAATAA CTAGGATTCG AATGAAAGTT GCCGTTTATT GTCGTGTTAG
     5941 CACCCTGGAA CAGAAAGAAC ATGGTCATAG CATTGAAGAA CAAGAGCGTA AACTGAAAAG
     6001 CTTCTGCGAT ATTAATGATT GGACCGTGTA TGATACCTAT ATCGATGCAG GTTATAGCGG
     6061 TGCAAAACGT GATCGTCCGG AACTGCAGCG TCTGATGAAT GATATTAACA AATTTGATCT
     6121 GGTGCTGGTG TATAAACTGG ATCGTCTGAC CCGTAATGTT CGTGATCTGC TGGACCTGCT
     6181 GGAAATCTTT GAAAAAAATG ATGTGAGCTT TCGTAGCGCC ACCGAAGTTT ATGATACCAC
     6241 CACCGCAATG GGTCGTCTGT TTGTTACCCT GGTTGGTGCA ATGGCAGAAT GGGAACGTGA
     6301 AACCATTCGT GAACGTACCC AGATGGGTAA ACTGGCAGCA CTGCGTAAAG GTATTATGCT
     6361 GACCACCCCT CCGTTTTATT ATGACCGTGT GGATAATAAG TTTGTGCCGA ACAAATACAA
     6421 AGACGTTATT CTGTGGGCAT ATGACGAAGC AATGAAAGGT CAGAGCGCAA AAGCAATTGC
     6481 ACGCAAACTG AATAATAGCG ATATTCCGCC TCCGAATAAT ACCCAGTGGC AGGGTCGTAC
     6541 CATTACCCAT GCCCTGCGTA ATCCGTTTAC CCGTGGTCAT TTTGATTGGG GTGGTGTGCA
     6601 TATTGAAAAT AACCATGAAC CGATCATCAC CGATGAGATG TATGAGAAAG TTAAAGATCG
     6661 CCTGAATGAA CGCGTGAACA CCAAAAAAGT TCGTCATACC AGCATTTTTC GTGGCAAACT
     6721 GGTTTGTCCG GTTTGTAATG CACGCCTGAC CCTGAATAGC CATAAAAAGA AAAGCAATAG
     6781 CGGCTATATC TTTGTGAAAC AGTACTACTG CAACAACTGT AAAGTTACCC CGAATCTGAA
     6841 ACCGGTGTAC ATCAAAGAAA AAGAAGTGAT TAAAGTTTTT TACAATTATC TGAAACGCTT
     6901 CGATCTGGAA AAATATGAGG TTACCCAGAA ACAGAACGAA CCGGAAATCA CCATCGATAT
     6961 CAATAAAGTT ATGGAACAGC GCAAACGCTA CCATAAACTG TATGCAAGCG GTCTGATGCA
     7021 AGAAGATGAA CTGTTTGACC TGATTAAAGA AACCGATCAG ACCATTGCCG AATATGAAAA
     7081 ACAGAATGAA AACCGCGAAG TGAAGCAGTA TGATATCGAA GATATCAAAC AGTATAAAGA
     7141 TCTGCTGTTA GAAATGTGGG ATATCAGCTC CGATGAAGAT AAAGAGGACT TTATCAAAAT
     7201 GGCGATTAAA AACATCTATT TTGAATATAT CATTGGCACC GGTAACACCA GCCGTAAACG
     7261 TAATAGCCTG AAAATTACGA GCATTGAATT CTATTAAcag ataaaaaaaa tccttagctt
     7321 tcgctaagga tgatttctGA TTAGGGTGAT GGTTCACGTA GTGGG 
//
