LOCUS       pRec07_10_08            8838 bp ds-DNA     circular     15-SEP-2014
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     New DNA(1) from 1 to 5890
COMMENT     New DNA from 1 to 5890
COMMENT     pRec07_ColE_Int7_optE6 from 1 to 5950
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2033..2716
                     /label=ColE1
                     /ApEinfo_fwdcolor=#4049ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             4498..5886
                     /label=Int7
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             5914..7359
                     /label=Int10
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             7385..8770
                     /label=Int8
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    190..1630
                     /label=KanR
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2717..2859
                     /label=spacer for Kan ColE1 frag
                     /ApEinfo_fwdcolor=#ebb8ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        4388..4402
                     /vntifkey="30"
                     /label=Pbad
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#00ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3258..4136)
                     /vntifkey="4"
                     /label=AraC
                     /ApEinfo_fwdcolor=#ff7a00
                     /ApEinfo_revcolor=#ffb16a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3056..3257)
                     /label=Spacer downstream of AraC
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=#00bbbb
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4337..4467
                     /label=Pbad BBa_10500
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8771..8811
                     /label=Term rrn
                     /ApEinfo_fwdcolor=#5c6bcf
                     /ApEinfo_revcolor=#5e85df
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 aactCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA
       61 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA
      121 TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA
      181 ATTTAACGCG AATTTTAACA AAATTTCACT GACACCCTCA TCAGTGCCAA CATAGTAAGC
      241 CAGTATACAC TCCGCTAGCG CTGAGGTCTG CCTCGTGAAG AAGGTGTTGC TGACTCATAC
      301 CAGGCCTGAA TCGCCCCATC ATCCAGCCAG AAAGTGAGGG AGCCACGGTT GATGAGAGCT
      361 TTGTTGTAGG TGGACCAGTT GGTGATTTTG AACTTTTGCT TTGCCACGGA ACGGTCTGCG
      421 TTGTCGGGAA GATGCGTGAT CTGATCCTTC AACTCAGCAA AAGTTCGATT TATTCAACAA
      481 AGCCACGTTG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAA TATATCATCA
      541 TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT
      601 CAACGGGAAA CGTCTTGCTC GAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT
      661 GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA TCGATTGTAT
      721 GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT
      781 GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC
      841 AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGGAAA
      901 ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG
      961 GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT
     1021 CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT
     1081 GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAG
     1141 CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT
     1201 ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC
     1261 CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG
     1321 AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT
     1381 TTGATGCTCG ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA CTGGCAGAGC
     1441 ATTACGCTGA CTTGACGGGA CTCGAGATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC
     1501 GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCCTGCATA ACGCGAAGTA
     1561 ATCTTTTCGG TTTTAAAGAA AAAGGGCAGG GTGGTGACAC CTTGCCCGTT TTTTTGCCGG
     1621 ACTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT
     1681 TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC
     1741 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT
     1801 CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC
     1861 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC
     1921 TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT
     1981 TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG
     2041 ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC
     2101 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA
     2161 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG
     2221 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA
     2281 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA
     2341 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG
     2401 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG
     2461 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG
     2521 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG
     2581 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC
     2641 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA
     2701 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG
     2761 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT
     2821 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGg tcggcgatat aggcgccagc
     2881 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatctgctc
     2941 atgtttgaca gcttatcatc gatgcataat gtgcctgtca aatggacgaa gcagggattc
     3001 tgcaaaccct atgctactcc ctcgagccgt caattgtctg attcgttacc aattatacta
     3061 gctccggcaa aaaaacgggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa
     3121 gattacttcg cgtttgccac ctgacgtcta agaaaaggaa tattcagcaa tttgcccgtg
     3181 ccgaagaaag gcccacccgt gaaggtgagc cagtgagttg attgctacgt aattagttag
     3241 ttagccctta gtgactctta tgacaacttg acggctacat cattcacttt ttcttcacaa
     3301 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag
     3361 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc
     3421 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct
     3481 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg
     3541 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt
     3601 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac
     3661 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg
     3721 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag
     3781 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga
     3841 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga
     3901 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga
     3961 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc
     4021 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc
     4081 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt
     4141 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt
     4201 cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc
     4261 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat
     4321 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca
     4381 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt
     4441 tctccatacc cgtttttttg ggctagcAGT AATTTCAACA AAATAACTAG GATTCGAATG
     4501 AAAGTGGCCA TTTATGTTCG TGTTAGCACC GATGAACAGG CCAAAGAAGG TTTTAGCATT
     4561 CCGGCACAGC GTGAACGTCT GCGTGCATTT TGTGCAAGCC AGGGTTGGGA AATTGTGCAA
     4621 GAATATATTG AAGAAGGTTG GAGCGCAAAA GATCTGGATC GTCCGCAGAT GCAGCGTCTG
     4681 CTGAAAGATA TCAAAAAAGG CAACATTGAT ATTGTGCTGG TGTATCGTCT GGATCGCCTG
     4741 ACCCGTAGCG TTCTGGATCT GTATCTGCTG CTGCAGACCT TTGAAAAATA CAATGTGGCA
     4801 TTTCGTAGCG CCACCGAAGT TTATGATACC AGCACCGCAA TGGGTCGTCT GTTTATTACC
     4861 CTGGTTGCAG CACTGGCACA GTGGGAACGT GAAAATCTGG CAGAACGTGT TAAATTTGGT
     4921 ATCGAGCAGA TGATCGATGA AGGTAAAAAA CCGGGTGGTC ATAGCCCGTA TGGTTACAAA
     4981 TTTGATAAAG ACTTCAATTG CACCATTATT GAGGAAGAAG CAGACGTTGT TCGTATGATC
     5041 TATCGCATGT ATTGTGATGG TTATGGCTAT CGTAGCATTG CAGATCGTCT GAATGAACTG
     5101 ATGGTTAAAC CGCGTATTGC CAAAGAATGG AATCATAATA GCGTGCGTGA TATCCTGACC
     5161 AACGATATCT ATATTGGCAC CTATCGTTGG GGTGATAAAG TTGTTCCGAA TAATCATCCG
     5221 CCTATTATTA GCGAAACCCT GTTCAAAAAA GCCCAGAAAG AAAAAGAAAA ACGTGGCGTT
     5281 GATCGTAAAC GCGTTGGTAA ATTTCTGTTT ACCGGTCTGC TGCAGTGTGG TAATTGTGGT
     5341 GGCCATAAAA TGCAGGGCCA TTTTGATAAA CGTGAGCAGA AAACCTATTA CCGTTGTACC
     5401 AAATGTCACC GCATTACCAA CGAAAAAAAC ATTCTGGAAC CGCTGCTGGA TGAAATTCAG
     5461 CTGCTGATTA CCAGCAAAGA ATACTTTATG AGCAAATTCA GCGACCGCTA TGATCAGCAA
     5521 GAGGTTGTTG ATGTTAGCGC ACTGACAAAA GAACTGGAAA AAATCAAACG CCAGAAAGAG
     5581 AAATGGTACG ATCTGTATAT GGATGATCGT AACCCGATTC CGAAAGAAGA ACTGTTTGCC
     5641 AAAATTAACG AACTGAACAA AAAAGAAGAA GAAATCTATA GCAAGCTGAG CGAAGTGGAA
     5701 GAAGATAAAG AACCGGTTGA AGAGAAATAT AACCGCCTGA GCAAAATGAT CGATTTTAAA
     5761 CAGCAGTTTG AGCAGGCCAA CGACTTTACC AAAAAAGAGC TGCTGTTCAG CATCTTCGAA
     5821 AAGATTGTGA TTTATCGCGA GAAAGGCAAG CTGAAAAAAA TCACCCTGGA TTACACCCTG
     5881 AAATAAtcca gtaTCAAACA GGAAGAAGGC TAGATGATCA CCACCAATAA AGTGGCGATT
     5941 TATGTGCGTG TTAGTACCAC CAATCAGGTT GAAGAGGGTT ATAGCATTGA TGAACAGAAA
     6001 GATAAACTGA GCAGCTATTG CGATATCAAA GATTGGAACG TGTACAAAGT GTATACCGAT
     6061 GGTGGTTTTA GCGGTAGCAA TACCGATCGT CCGGCACTGG AAAGCCTGAT TAAAGATGCA
     6121 AAAAAACGCA AATTCGATAC CGTGCTGGTG TATAAACTGG ATCGTCTGAG CCGTAGCCAG
     6181 AAAGACACCC TGCATCTGAT TGAAGATGTG TTTATCAAAA ATGGCATTGA ATTTCTGAGC
     6241 CTGCAAGAGA ATTTTGATAC CAGCACCCCG TTTGGTAAAG CAATGATTGG TCTGCTGAGC
     6301 GTTTTTGCAC AGCTGGAACG TGAGCAGATT AAAGAACGTA TGCAGCTGGG TAAACTGGGT
     6361 CGTGCAAAAA GCGGTAAAAG CATGATGTGG GCAAAAACCA GCTATGGCTA TGATTATCAC
     6421 AAAGAAACCG GCACCGTTAC CATTAATCCG GCACAGGCAC TGACCATCAA ATTTATCTTT
     6481 GAAAGCTATC TGCGTGGTCG CAGCATTACC AAACTGCGTG ATGATCTGAA TGAGAAATAT
     6541 CCGAAACATG TGCCGTGGTC ATATCGTGCA GTTCGTACCA TTCTGGATAA TCCGGTTTAT
     6601 TGCGGTTTCA ACCAGTATAA AGGTGAAATC TATCCGGGTA ACCATGAACC GATTATTAGC
     6661 AAAGAAGAGT ACGATAAAAC CCAGAGCGAA CTGAAAATTC GTCAGCGTAC CGCAGCAGAA
     6721 AATGTTAATC CGCGTCCGTT TCAGGCCAAA TATATCCTGA GCGGTATTGC CCAGTGTGGT
     6781 TATTGTGGTG CACCGCTGAA AATCATGCTG GGTGTTAAAC GTAAAGATGG TAGCCGTCTG
     6841 AAAAAGTATG AATGTCATCA GCGTCATCCG CGTACCCTGC GTGGTGTTAC CACCTATAAT
     6901 GATAACAAAA AATGCGACAG CGGCTTTTAC TACAAAGACA AACTGGAAGC CTATGTGCTG
     6961 AAAGAAATTA GCAAACTGCA GGACGATGCA GATTATCTGG ATAAAATCTT TAGCGGTGAT
     7021 AACGCCGAAA CCATTGATCG TGAGAGCTAT AAAAAGCAGA TCGAGGAACT GAGCAAAAAA
     7081 CTGAGTCGTC TGAACGATCT GTATATCGAT GATCGTATTA CCCTGGAAGA ACTGCAGAGC
     7141 AAAAGCGCAG AATTTATCAG CATGCGTGGC ACCCTGGAAA CCGAACTGGA AAATGATCCT
     7201 GCACTGCGTA AAAACAAACG GAAAGCAGAT ATGCGTAAAC TGCTGAATGC CGAAAAAGTG
     7261 TTCAGCATGG ATTATGAGAG TCAGAAAGTT CTGGTTCGTC GCCTGATCAA TAAAGTTAAA
     7321 GTTACCGCTG AAGATATTGT GATTAATTGG AAAATCTAAg aatAAAACAA TAACTAGGAT
     7381 TCGAATGAAA GTTGCCGTTT ATTGTCGTGT TAGCACCCTG GAACAGAAAG AACATGGTCA
     7441 TAGCATTGAA GAACAAGAGC GTAAACTGAA AAGCTTCTGC GATATTAATG ATTGGACCGT
     7501 GTATGATACC TATATCGATG CAGGTTATAG CGGTGCAAAA CGTGATCGTC CGGAACTGCA
     7561 GCGTCTGATG AATGATATTA ACAAATTTGA TCTGGTGCTG GTGTATAAAC TGGATCGTCT
     7621 GACCCGTAAT GTTCGTGATC TGCTGGACCT GCTGGAAATC TTTGAAAAAA ATGATGTGAG
     7681 CTTTCGTAGC GCCACCGAAG TTTATGATAC CACCACCGCA ATGGGTCGTC TGTTTGTTAC
     7741 CCTGGTTGGT GCAATGGCAG AATGGGAACG TGAAACCATT CGTGAACGTA CCCAGATGGG
     7801 TAAACTGGCA GCACTGCGTA AAGGTATTAT GCTGACCACC CCTCCGTTTT ATTATGACCG
     7861 TGTGGATAAT AAGTTTGTGC CGAACAAATA CAAAGACGTT ATTCTGTGGG CATATGACGA
     7921 AGCAATGAAA GGTCAGAGCG CAAAAGCAAT TGCACGCAAA CTGAATAATA GCGATATTCC
     7981 GCCTCCGAAT AATACCCAGT GGCAGGGTCG TACCATTACC CATGCCCTGC GTAATCCGTT
     8041 TACCCGTGGT CATTTTGATT GGGGTGGTGT GCATATTGAA AATAACCATG AACCGATCAT
     8101 CACCGATGAG ATGTATGAGA AAGTTAAAGA TCGCCTGAAT GAACGCGTGA ACACCAAAAA
     8161 AGTTCGTCAT ACCAGCATTT TTCGTGGCAA ACTGGTTTGT CCGGTTTGTA ATGCACGCCT
     8221 GACCCTGAAT AGCCATAAAA AGAAAAGCAA TAGCGGCTAT ATCTTTGTGA AACAGTACTA
     8281 CTGCAACAAC TGTAAAGTTA CCCCGAATCT GAAACCGGTG TACATCAAAG AAAAAGAAGT
     8341 GATTAAAGTT TTTTACAATT ATCTGAAACG CTTCGATCTG GAAAAATATG AGGTTACCCA
     8401 GAAACAGAAC GAACCGGAAA TCACCATCGA TATCAATAAA GTTATGGAAC AGCGCAAACG
     8461 CTACCATAAA CTGTATGCAA GCGGTCTGAT GCAAGAAGAT GAACTGTTTG ACCTGATTAA
     8521 AGAAACCGAT CAGACCATTG CCGAATATGA AAAACAGAAT GAAAACCGCG AAGTGAAGCA
     8581 GTATGATATC GAAGATATCA AACAGTATAA AGATCTGCTG TTAGAAATGT GGGATATCAG
     8641 CTCCGATGAA GATAAAGAGG ACTTTATCAA AATGGCGATT AAAAACATCT ATTTTGAATA
     8701 TATCATTGGC ACCGGTAACA CCAGCCGTAA ACGTAATAGC CTGAAAATTA CGAGCATTGA
     8761 ATTCTATTAA cagataaaaa aaatccttag ctttcgctaa ggatgatttc tGATTAGGGT
     8821 GATGGTTCAC GTAGTGGG    
//
