LOCUS pCA_Myc_Kir2_1EY_2 7808 bp ds-DNA circular 02-JUN-2011 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT CJ-PCA-T7_F07.ab1 basecalls COMMENT ApEinfo:methylated:0 FEATURES Location/Qualifiers primer_bind complement(5057..5077) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 1893..1921 /label=Myc tag /ApEinfo_fwdcolor=#46ff35 /ApEinfo_revcolor=green rep_origin complement(7345..7651) /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 1892..1921 /label=Myc tag(1) /ApEinfo_label=Myc tag /ApEinfo_fwdcolor=#46ff35 /ApEinfo_revcolor=green primer_bind complement(5020..5039) /label=T3 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind 1..18 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 2642..2644 /label=Y242F /ApEinfo_fwdcolor=#ff72cd /ApEinfo_revcolor=green primer_bind complement(5060..5077) /label=M13-rev(1) /ApEinfo_label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 3203..3265 /label=2A /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind 27..47 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin complement(5445..6127) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 rep_origin 7223..7663 /label=F1 ori(1) /ApEinfo_label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 CDS complement(7670..7738) /label=LacZ alpha /ApEinfo_fwdcolor=cornflower blue /ApEinfo_revcolor=cornflower blue misc_binding complement(5083..5105) /label=LacO /ApEinfo_fwdcolor=cornflower blue /ApEinfo_revcolor=cornflower blue CDS complement(6225..6884) /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow polyA_signal 4864..4869 /label=polyA signal /ApEinfo_fwdcolor=#fc2e2e /ApEinfo_revcolor=green polyA_signal 4893..4898 /label=polyA signal(1) /ApEinfo_label=polyA signal /ApEinfo_fwdcolor=#fc2e2e /ApEinfo_revcolor=green polyA_signal 6396..6401 /label=polyA signal(2) /ApEinfo_label=polyA signal /ApEinfo_fwdcolor=#fc2e2e /ApEinfo_revcolor=green polyA_signal 7165..7170 /label=polyA signal(3) /ApEinfo_label=polyA signal /ApEinfo_fwdcolor=#fc2e2e /ApEinfo_revcolor=green polyA_signal complement(565..570) /label=polyA signal(4) /ApEinfo_label=polyA signal /ApEinfo_fwdcolor=#fc2e2e /ApEinfo_revcolor=green polyA_signal complement(569..574) /label=polyA signal(5) /ApEinfo_label=polyA signal /ApEinfo_fwdcolor=#fc2e2e /ApEinfo_revcolor=green polyA_signal complement(4839..4844) /label=polyA signal(6) /ApEinfo_label=polyA signal /ApEinfo_fwdcolor=#fc2e2e /ApEinfo_revcolor=green misc_feature 132..1846 /label=CMV-IE/CbA promoter /ApEinfo_fwdcolor=#65c6eb /ApEinfo_revcolor=green misc_feature 3281..4711 /label=tdTomato /ApEinfo_fwdcolor=#ff7477 /ApEinfo_revcolor=green misc_feature 3266..3267 /label=extra 2 bps to make in-frame /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green misc_feature 2588..2590 /label=E224G /ApEinfo_fwdcolor=#ff0fcd /ApEinfo_revcolor=green misc_feature 1865..1870 /label=XmaI /ApEinfo_fwdcolor=#ff0d75 /ApEinfo_revcolor=green misc_feature 3268..3273 /label=BamHI /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 1869..1874 /label=BamHI(1) /ApEinfo_label=BamHI /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 2348..2356 /label=GYG144-146 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 4712..4717 /label=EcoRI /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green ORIGIN 1 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc 61 gggccccccc tcgaggtcga cggtATCGAT AAGCTTCCCT TAATTAACCG TTTAAACAAT 121 TCTGCAGGAA Tctagttatt aatagtaatc aattacgggg tcattagttc atagcccata 181 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 241 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 301 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 361 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 421 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 481 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc 541 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg 601 ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg 661 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt 721 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcggggag 781 tcgctgcgac gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc 841 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg 901 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc 961 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg 1021 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc 1081 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc 1141 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc 1201 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc 1261 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc 1321 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1381 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc 1441 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag 1501 ggacttcctt tgtcccaaat CTGTGCGGAG CCGAAATCTG Ggaggcgccg ccgcaccccc 1561 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc 1621 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg 1681 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc 1741 ggcggctcta GAGCCTCTGC TAACCATGTT CATGCcttct tctttttcct acagctcctG 1801 GGCAACGTGC TGGTTATTGT Gctgtctcat cattttggca aagaatTTGA TTTGATACCG 1861 CGGGcccggg atccgcggcc gcgccaccAT GGAGCAGAAG CTGATCAGCG AGGAGGACCT 1921 GGGCAGTGTG AGAACCAACC GGTACAGCAT TGTCTCTTCG GAGGAAGATG GCATGAAGCT 1981 GGCCACTATG GCAGTTGCCA ATGGCTTTGG GAATGGCAAG AGTAAAGTCC ATACCCGACA 2041 ACAGTGCAGG AGCCGCTTTG TGAAGAAAGA TGGTCATTGC AATGTTCAGT TTATCAACGT 2101 GGGTGAGAAG GGACAGAGGT ACCTGGCAGA CATCTTTACT ACCTGTGTCG ACATCCGCTG 2161 GAGGTGGATG CTGGTTATCT TCTGTCTTGC CTTCGTGCTC TCCTGGCTGT TCTTTGGCTG 2221 TGTGTTTTGG TTGATAGCCC TGCTCCATGG GGATCTAGAT ACTTCTAAAG TGAGCAAAGC 2281 ATGCGTGTCG GAGGTTAACA GCTTCACGGC TGCCTTCCTC TTCTCCATCG AGACCCAGAC 2341 AACCATTGGC TATGGTTTCA GGTGTGTGAC AGACGAATGC CCAATTGCTG TCTTCATGGT 2401 GGTATTCCAG TCAATTGTAG GCTGCATCAT TGACGCCTTC ATCATTGGTG CAGTCATGGC 2461 GAAGATGGCA AAGCCAAAGA AGAGAAATGA GACTCTTGTC TTCAGTCACA ATGCTGTGAT 2521 TGCCATGAGG GATGGCAAAC TCTGCTTGAT GTGGAGAGTG GGTAACCTTC GAAAGAGCCA 2581 CCTTGTGGGA GCTCATGTCC GGGCACAGCT TCTCAAATCT AGGATCACTT CAGAAGGGGA 2641 GTTTATCCCT TTGGACCAGA TAGACATCAA TGTTGGTTTT GATAGTGGAA TTGACCGCAT 2701 ATTTCTAGTG TCCCCCATCA CTATCGTTCA CGAAATAGAT GAAGACAGCC CTTTATATGA 2761 CTTGAGTAAG CAGGACATTG ACAATGCAGA CTTTGAAATT GTTGTCATAC TGGAAGGCAT 2821 GGTGGAGGCG ACTGCCATGA CAACTCAATG CCGGAGTTCG TATCTGGCCA ATGAAATTCT 2881 CTGGGGTCAC CGCTATGAGC CAGTGCTCTT TGAAGAGAAA CACTACTATA AAGTAGACTA 2941 TTCAAGATTC CATAAGACTT ATGAAGTACC TAACACCCCC CTTTGTAGTG CCAGAGACTT 3001 AGCAGAGAAG AAATACATCC TTTCAAATGC AAATTCATTT TGCTATGAAA ATGAAGTTGC 3061 CCTAACAAGC AAAGAGGAAG AGGAGGATAG TGAGAACGGA GTCCCAGAGA GCACAAGCAC 3121 AGACTCACCT CCTGGCATAG ATCTCCACAA CCAGGCAAGC GTACCTCTAG AGCCCAGGCC 3181 CTTAAGGCGA GAATCGGAGA TAGGCAGTGG AGAGGGCAGA GGAAGTCTGC TAACATGCGG 3241 TGACGTCGAG GAGAATCCTG GCCCAgcgGA TCCcgccacc ATGGTGAGCA AGGGCGAGGA 3301 GGtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga 3361 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct 3421 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat 3481 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc 3541 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac 3601 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg 3661 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc 3721 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa 3781 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc 3841 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga 3901 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctggg 3961 gcatggcacc ggcagcaccg gcagcggcag ctccggcacc gcctcctccg aggacaacaa 4021 catggccgtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg 4081 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 4141 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca 4201 gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa 4261 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct 4321 ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat 4381 gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga 4441 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc 4501 cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa 4561 gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca 4621 caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt 4681 cctgtacggc atggacgagc tgtacaagta aGAATTCTAG ATAACTGATC ATAATCAGCC 4741 ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC 4801 TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT 4861 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 4921 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATTTAAA TTGGGCGCGC CCAACTAGTT 4981 CTAGAGCGGC CGCcaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt 5041 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 5101 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 5161 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 5221 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 5281 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 5341 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 5401 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 5461 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 5521 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 5581 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 5641 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 5701 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 5761 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 5821 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 5881 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 5941 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 6001 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 6061 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 6121 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 6181 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 6241 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 6301 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 6361 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 6421 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 6481 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 6541 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 6601 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 6661 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 6721 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 6781 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 6841 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 6901 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 6961 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 7021 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 7081 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 7141 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 7201 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt 7261 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag 7321 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga 7381 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg 7441 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc 7501 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg 7561 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc 7621 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat 7681 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 7741 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 7801 cacgacgt //