LOCUS       pENTR-D-TOPO-PAm        3357 bp    DNA     circular     24-JAN-2010
DEFINITION  attL1-attL2.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|510835872|
COMMENT     VNTDBDATE|544032382|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENTR-D-TOPO-PAmCherry1-MCS|
COMMENT     VNTAUTHORNAME|Xiaolin Nan|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pENTR-D-TOPO-PAmCherry1-MCS" 0 3357 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 1 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 40 50 0 1 0 
COMMENT        (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "BamHI" "EcoRI" "HindIII" "NdeI" "SalI" "BglII" "XbaI" 
COMMENT         "PstI" "AvaI" "XhoI" "NotI" "SacI" "KpnI" "NcoI" "EcoRV" "SpeI" "ApaI" 
COMMENT         "AgeI" "BstBI" "MluI" "ClaI" "SnaBI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 
COMMENT        "Courier New" 16 0 0 1 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 3357 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 3323 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3317 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2555 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NdeI" "catatg" 3 0 2 2649 0 3300 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 3311 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BglII" "agatct" 2 0 1 3293 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 2571 0 2940 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 4 2426 0 2561 0 2861 0 3053 
COMMENT            0 0 "") #9=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2561 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2538 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 2577 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 2583 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 2583 0 3018 0 3305 0 0 
COMMENT             "") #14=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 103 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 2433 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "MluI" "acgcgt" 2 0 2 1164 0 2096 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 0 0 "")) 
COMMENT       (CObList 
COMMENT        #22=(CFSignal (CObList) "rrnB T2 transcription terminator" 43 0 0 2133 
COMMENT             2160 0 (CStringList) (CStringList "/invitrogen=\"2590000\"") 1 1 1 
COMMENT             1 "268..295") 
COMMENT        #23=(CFSignal (CObList) "rrnB T1 transcription terminator" 43 0 0 2292 
COMMENT             2335 0 (CStringList) (CStringList "/invitrogen=\"2580000\"") 1 1 1 
COMMENT             1 "427..470") 
COMMENT        #24=(CFSignal (CObList) "T7 promoter" 30 0 1 109 125 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2370000\"") 1 1 1 1 
COMMENT             "complement(3006..3022)") 
COMMENT        #25=(CFSignal (CObList) "T7 primer" 27 0 1 106 125 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2100000\"") 1 1 1 1 
COMMENT             "complement(3003..3022)") 
COMMENT        #26=(CFSignal (CObList) "M13 reverse primer" 27 0 1 130 146 0 
COMMENT             (CStringList) (CStringList "/invitrogen=\"2010000\"") 1 1 1 1 
COMMENT             "complement(3027..3043)") 
COMMENT        #27=(CFSignal (CObList) "M13 (-40) forward primer" 27 0 0 2382 2398 0 
COMMENT             (CStringList) (CStringList "/invitrogen=\"2000000\"") 1 1 1 1 
COMMENT             "517..533") 
COMMENT        #28=(CFSignal (CObList) "M13 (-20) forward primer" 27 0 0 2402 2417 0 
COMMENT             (CStringList) (CStringList "/invitrogen=\"1980000\"") 1 1 1 1 
COMMENT             "537..552") 
COMMENT        #29=(CFSignal (CObList) "Kan(R)" 4 0 0 259 1068 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1210000\"") 1 1 1 1 "3156..3965") 
COMMENT        #30=(CFSignal (CObList) "PAmCherry1" 4 0 0 2584 3291 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                          "DNA 'pENTR-D-TOPO-PAmCherry1-MCS'" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "attL1-attL2" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 02/24/09 10:51AM" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/24/10 04:06PM" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 3357 bp" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                   "Standard Fields") 
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COMMENT                   "Annotations") 
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COMMENT                          "TGGCCA") 
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COMMENT                                        " N1: 2433 " 1))))) "ApaI: 1 site") 5 1 
COMMENT                          "GGGCCC" "CCCGGG") 
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COMMENT                            (CObjectList)) "AvaI: 4 sites") 1 5 "CYCGRG" 
COMMENT                          "GRGCYC") 
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COMMENT                                        " N1: 3323 " 1))))) "BamHI: 1 site") 1 5 
COMMENT                          "GGATCC" "CCTAGG") 
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COMMENT                                        " N1: 3293 " 1))))) "BglII: 1 site") 1 5 
COMMENT                          "AGATCT" "TCTAGA") 
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COMMENT                          "TAGCTA") 
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COMMENT                          "GAATTC" "CTTAAG") 
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COMMENT                                        " N1: 103 " 1))))) "EcoRV: 1 site") 3 3 
COMMENT                          "GATATC" "CTATAG") 
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COMMENT                                        " N1: 2555 " 1))))) "HindIII: 1 site") 1 
COMMENT                          5 "AAGCTT" "TTCGAA") 
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COMMENT                                        " N1: 2583 " 1))))) "KpnI: 1 site") 5 1 
COMMENT                          "GGTACC" "CCATGG") 
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COMMENT                           (CGroupPar (CParagraph 77013408 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "MluI: 2 sites") 1 5 "ACGCGT" 
COMMENT                          "TGCGCA") 
COMMENT                     #71=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 76990720 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "NcoI: 3 sites") 1 5 "CCATGG" 
COMMENT                          "GGTACC") 
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COMMENT                          "GTATAC") 
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COMMENT                          "GCGGCCGC" "CGCCGGCG") 
COMMENT                     #76=(CRSFolderPar 
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COMMENT                           (CGroupPar (CParagraph 76984320 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "PstI: 2 sites") 5 1 "CTGCAG" 
COMMENT                          "GACGTC") 
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COMMENT                          "GAGCTC" "CTCGAG") 
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COMMENT                                        " N1: 3311 " 1))))) "SalI: 1 site") 1 5 
COMMENT                          "GTCGAC" "CAGCTG") 
COMMENT                     #83=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 77013248 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "SnaBI: 0 sites") 3 3 "TACGTA" 
COMMENT                          "ATGCAT") 
COMMENT                     #84=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 77013536 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "SpeI: 0 sites") 1 5 "ACTAGT" 
COMMENT                          "TGATCA") 
COMMENT                     #85=(CRSFolderPar 
COMMENT                          (CFolderPar 
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COMMENT                            (CObjectList)) "XbaI: 0 sites") 1 5 "TCTAGA" 
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COMMENT                                        " N1: 2561 " 1))))) "XhoI: 1 site") 1 5 
COMMENT                          "CTCGAG" "GAGCTC"))) 
COMMENT                   "Restriction/Methylation Map:  (no more than 1 site)") 
COMMENT              #89=(CIvgnResourcePar 
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COMMENT                    "<a href=\"ivgnlink:/iq\">Invitrogen products related to pENTR-D-TOPO-PAmCherry1-MCS</a>" 
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COMMENT              #90=(CRefLinePar 
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COMMENT                                   #96=(CLinePar 
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COMMENT                                        "Start: 2584  End: 3291" 1))) 
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COMMENT                                          "Start: 106   End: 125  (Complementary)" 
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COMMENT                                          "Start: 130   End: 146  (Complementary)" 
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COMMENT                                  "M13 reverse primer") 
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COMMENT                                          "   537..552" 1))) 
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COMMENT                                           "   complement(3006..3022)" 1))) 
COMMENT                                   "T7 promoter"))) 
COMMENT                           "Promoter Prokaryotic (1 total)") 
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COMMENT                                           "   427..470" 1))) 
COMMENT                                   "rrnB T1 transcription terminator"))) 
COMMENT                           "Terminator (2 total)"))) "Feature Map")))) 
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FEATURES             Location/Qualifiers
     terminator      2133..2160
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      2292..2335
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     promoter        complement(109..125)
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     primer          complement(106..125)
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     primer          complement(130..146)
                     /invitrogen="2010000"
                     /vntifkey="27"
                     /label=M13\reverse\primer
     primer          2382..2398
                     /invitrogen="2000000"
                     /vntifkey="27"
                     /label=M13\(-40)\forward\primer
     primer          2402..2417
                     /invitrogen="1980000"
                     /vntifkey="27"
                     /label=M13\(-20)\forward\primer
     CDS             259..1068
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     CDS             2584..3291
                     /vntifkey="4"
                     /label=PAmCherry1
BASE COUNT      825 a       866 c       872 g       794 t 
ORIGIN
        1 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc 
       61 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg 
      121 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt 
      181 tacattgcac aagataaaat aatatcatca tgaacaataa aactgtctgc ttacataaac 
      241 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat 
      301 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca 
      361 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat 
      421 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg 
      481 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta 
      541 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca 
      601 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt 
      661 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg 
      721 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa 
      781 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat 
      841 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat 
      901 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc 
      961 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct 
     1021 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt 
     1081 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga 
     1141 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag 
     1201 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     1261 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     1321 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 
     1381 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     1441 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
     1501 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
     1561 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
     1621 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
     1681 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
     1741 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
     1801 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 
     1861 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 
     1921 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 
     1981 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 
     2041 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt 
     2101 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct 
     2161 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt 
     2221 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg 
     2281 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat 
     2341 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt 
     2401 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata 
     2461 gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac 
     2521 aaaaaagcag gctccgcggc cgcccccttc accaagcttc tcgagctgca ggagctcggt 
     2581 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc 
     2641 aaggtgcata tggagggctc cgtgaacggc cacgtgttcg agatcgaggg cgagggcgag 
     2701 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg 
     2761 cccttcacct gggacatcct gtcccctcag ttcatgtacg gctccaacgc ctacgtgaag 
     2821 caccccgccg acatccccga ctacttcaag ctgtccttcc ccgagggctt caagtgggag 
     2881 cgcgtgatga agttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag 
     2941 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc 
     3001 gtaatgcaga agaagaccat gggctgggag gccctctccg agcggatgta ccccgaggac 
     3061 ggcgccctga agggcgaggt caagccaagg gtcaagctga aggacggcgg ccactacgac 
     3121 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac 
     3181 gtcaaccgca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac 
     3241 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gagatctcat 
     3301 atgccatggg tcgacgaatt cggatcctaa aagggtgggc gcgccgaccc agctttc 
//
