LOCUS       pYUM10001\(pTriE        6429 bp    DNA     circular     23-JUL-2014
DEFINITION  pTriEx-1 replacement.
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|652646355|
COMMENT     VNTDBDATE|704300985|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pYUM10001 (pTriEx1.1 mCherry-NES1-LOVwt-NLS)|
COMMENT     VNTAUTHORNAME|Hayretin Yumerefendi|
COMMENT     VNTAUTHOREML|yumer@email.unc.edu|
COMMENT     VNTOAUTHORNAME|Keith W. Yaeger|
COMMENT     VNTOAUTHORTEL|(608) 238-6110  x133|
COMMENT     VNTOAUTHORFAX|(608) 238-1388|
COMMENT     VNTOAUTHOREML|keith.yaeger@novagen.com|
COMMENT     VNTOAUTHORAD1|Novagen|
COMMENT     VNTOAUTHORAD2|601 Science Dr.|
COMMENT     VNTOAUTHORAD3|Madison,  WI  53711|
COMMENT     VNTOAUTHORAD4|U.S.A.|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     enhancer        1079..1443
                     /vntifkey="9"
                     /label=CMV\Enhancer
     promoter        1449..1726
                     /vntifkey="29"
                     /label=Chicken\B-Actin\Promoter
     enhancer        45..1011
                     /vntifkey="9"
                     /label=Lef2,603
     exon            2082..2149
                     /vntifkey="61"
                     /label=Exon\3\(Rabbit\Globin)
     polyA_signal    3646..3928
                     /vntifkey="25"
                     /label=Rabbit\B-Globin\Poly\A
     misc_signal     3992..4697
                     /vntifkey="87"
                     /label=1629
     rep_origin      complement(4908..4908)
                     /vntifkey="33"
                     /label=COLE1_ORI
     CDS             complement(5499..6367)
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    3569..3604
                     /vntifkey="21"
                     /label=HSV-Tag
     misc_feature    3611..3634
                     /vntifkey="21"
                     /label=His-Tag
     promoter        2150..2166
                     /vntifkey="30"
                     /label=T7\Promoter
     protein_bind    2171..2191
                     /vntifkey="31"
                     /label=lac\Operator
     terminator      3932..3979
                     /vntifkey="43"
                     /label=T7_TERM
     promoter        2205..2318
                     /vntifkey="29"
                     /label=p10\promoter\+\5'\UTR
     misc_feature    3551..3566
                     /vntifkey="21"
                     /label=3-ORF\Site
     CDS             2333..3040
                     /vntifkey="4"
                     /label=mCherry
     CDS             3095..3532
                     /vntifkey="4"
                     /label=LOV-NLS
     misc_feature    3047..3076
                     /vntifkey="21"
                     /label=NES1
BASE COUNT     1643 a      1521 c      1549 g      1716 t 
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga 
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat 
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca 
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa 
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt 
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa 
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag 
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt 
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt 
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt 
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg 
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc 
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt 
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt 
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc 
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg 
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccg 
     1021 taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctct 
     1081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 
     1141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 
     1201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 
     1261 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 
     1321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 
     1381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 
     1441 atgcatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc 
     1501 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg 
     1561 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg 
     1621 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag 
     1681 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg 
     1741 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact 
     1801 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctccttcgg gctgtaatta 
     1861 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct 
     1921 ccgggagggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg gacggctgcc 
     1981 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag 
     2041 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg 
     2101 ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt aatacgactc 
     2161 actatagggg aattgtgagc ggataacaat tccccggagt taatccggga cctttaattc 
     2221 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat 
     2281 taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata ccatggtgag 
     2341 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat 
     2401 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta 
     2461 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg 
     2521 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga 
     2581 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa 
     2641 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt 
     2701 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccctg taatgcagaa 
     2761 gaagactatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa 
     2821 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa 
     2881 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa 
     2941 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga 
     3001 gggccgccac tccaccggcg gcatggacga gctgtacaag ggtaccctgg cgctgaaact 
     3061 ggcgggcctg gatattggtt ctggaagtgg atccttggct actacacttg aacgtattga 
     3121 gaagaacttt gtcattactg acccaagatt gccagataat cccattatat tcgcgtccga 
     3181 tagtttcttg cagttgacag aatatagccg tgaagaaatt ttgggaagaa actgcaggtt 
     3241 tctacaaggt cctgaaactg atcgcgcgac agtgagaaaa attagagatg ccatagataa 
     3301 ccaaacagag gtcactgttc agctgattaa ttatacaaag agtggtaaaa agttctggaa 
     3361 cctctttcac ttgcagccta tgcgagatca gaagggagat gtccagtact ttattggggt 
     3421 tcagttggat ggaactgagc atgtccgaga tgctgccgag agagagggag tcatgctgat 
     3481 taagaaaact gcagaaaata ttgatatggc ggcaaaaaga gtcaaactgg attaaaagct 
     3541 tgcggccgca cagctgtata cacgtgcaag ccagccagaa ctcgccccgg aagaccccga 
     3601 ggatctcgag caccaccatc accatcacca tcactaagtg attaacctca ggtgcaggct 
     3661 gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat 
     3721 cgatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac 
     3781 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct 
     3841 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt 
     3901 ttagagtttg gcaacatatg cccatatgta actagcataa ccccttgggg cctctaaacg 
     3961 ggtcttgagg ggttttttgc tgaaagcatg cggaggaaat tctccttgaa gtttccctgg 
     4021 tgttcaaagt aaaggagttt gcaccagacg cacctctgtt cactggtccg gcgtattaaa 
     4081 acacgataca ttgttattag tacatttatt aagcgctaga ttctgtgcgt tgttgattta 
     4141 cagacaattg ttgtacgtat tttaataatt cattaaattt ataatcttta gggtggtatg 
     4201 ttagagcgaa aatcaaatga ttttcagcgt ctttatatct gaatttaaat attaaatcct 
     4261 caatagattt gtaaaatagg tttcgattag tttcaaacaa gggttgtttt tccgaaccga 
     4321 tggctggact atctaatgga ttttcgctca acgccacaaa acttgccaaa tcttgtagca 
     4381 gcaatctagc tttgtcgata ttcgtttgtg ttttgttttg taataaaggt tcgacgtcgt 
     4441 tcaaaatatt atgcgctttt gtatttcttt catcactgtc gttagtgtac aattgactcg 
     4501 acgtaaacac gttaaataga gcttggacat atttaacatc gggcgtgtta gctttattag 
     4561 gccgattatc gtcgtcgtcc caaccctcgt cgttagaagt tgcttccgaa gacgattttg 
     4621 ccatagccac acgacgccta ttaattgtgt cggctaacac gtccgcgatc aaatttgtag 
     4681 ttgagctttt tggaattgcg atcgcataac ttcgtatagc atacattata cgaagttata 
     4741 agctcggaac gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 
     4801 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 
     4861 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 
     4921 gccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 
     4981 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 
     5041 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 
     5101 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 
     5161 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 
     5221 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 
     5281 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 
     5341 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 
     5401 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 
     5461 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgttac caatgcttaa 
     5521 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 
     5581 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 
     5641 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 
     5701 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 
     5761 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 
     5821 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 
     5881 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 
     5941 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 
     6001 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 
     6061 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 
     6121 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 
     6181 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 
     6241 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 
     6301 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 
     6361 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatgt 
     6421 ccgcgcgtt 
//
