LOCUS       Exported                4566 bp ds-DNA    circular SYN 19-SEP-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pIS1 EPAS1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 19, 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1442..1561
                     /note="SV40 poly(A) site"
     CDS             1804..2661
                     /codon_start=1
                     /note="open reading frame#3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    4033..4249
                     /note="TK Promoter"
                     /note="TK promoter
                     "
     CDS             4524..5456
                     /codon_start=1
                     /note="open reading frame#2"
                     /translation="?P?GGEGGGEG?GGGGGGGGGGGGGGGGGGREGGGGGEGGGE?GG
                     GGGGGGGREGGGGGGGGGAGGGGGG?GGGGGGGPGGGGG?GGG?GGGGGGEGGGGGGGG
                     GGGGGGGGGEGGGGGGRGGGGGGGGGGGGGGGGGRGGG?GGEGGGGGGGGGGGGGGGGG
                     EGGRGRGSERGRGGGR?GARE?GGAR??E??GERGGE?E?A???PPEG?RE?ERG?ERE
                     GPR?R?PGRP??R?RER?G?G??E?E?EREPGRAGERER???GAREGE?EPA??PERE?
                     ?PERP?P?PRPPAPGP?G?P??G??RPP?P"
ORIGIN
        1 taactggtcc gcagtggtgg gccagatgta aacaaatgaa tgttcttgat tcatttatta
       61 attattatga ttcagaaaaa catgcagaaa atgctgttat ttttttacat ggtaacgcgg
      121 cctcttctta tttatggcga catgttgtgc cacatattga gccagtagcg cggtgtatta
      181 taccagacct tattggtatg ggcaaatcag gcaaatctgg taatggttct tataggttac
      241 ttgatcatta caaatatctt actgcatggt ttgaacttct taatttacca aagaagatca
      301 tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca ttatagctat gagcatcaag
      361 ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa tcatgggatg
      421 aatggcctga tattgaagaa gatattgcgt tgatcaaatc tgaagaagga gaaaaaatgg
      481 ttttggagaa taacttcttc gtggaaacca tgttgccatc aaaaatcatg agaaagttag
      541 aaccagaaga atttgcagca tatcttgaac cattcaaaga gaaaggtgaa gttcgtcgtc
      601 caacattatc atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct gacgttgtac
      661 aaattgttag gaattataat gcttatctac gtgcaagtga tgatttacca aaaatgttta
      721 ttgaatcgga cccaggattc ttttccaatg ctattgttga aggtgccaag aagtttccta
      781 atactgaatt tgtcaaagta aaaggtcttc atttttcgca agaagatgca cctgatgaaa
      841 tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa taattctagg
      901 agctctatac cggtctcgat atcactacta gtgttctaga ctttgcaact ccctgggtaa
      961 gagggacgac acctctggtt tttcaatacc aattacatgg aacttttctg taatgggtac
     1021 aatgaagaag tttctaaaaa cacacacaaa gcacattggg ccaactattt agtaagcccg
     1081 gatagactta ttgccaaaaa caaaaaatag ctttcaaaag aaatttaagt tctatgagaa
     1141 attccttagt catggtgttg cgtaaatcat attttagctg cacggcatta ccccacacag
     1201 ggtggcagaa cttgaagggt tactgacgtg taaatgctgg tatttgattt cctgtgtgtg
     1261 ttgccctggc attaagggca ttttaccctt gcagttttac taaaacactg aaaaatattc
     1321 caagcttcat attaacccta cctgtcaacg taacgatttc atgaacgtta ttatattgtc
     1381 gaattcctac tgacaacatt ataactgtat gggagcttaa cgcggccgct tcgagcagac
     1441 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     1501 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     1561 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     1621 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccaggtggc
     1681 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     1741 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1801 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1861 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1921 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1981 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     2041 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     2101 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     2161 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     2221 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     2281 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     2341 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     2401 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     2461 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     2521 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     2581 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     2641 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     2701 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2761 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2821 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2881 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2941 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3001 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3061 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3121 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3181 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3241 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3301 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3361 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3421 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3481 atggctcgac agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta
     3541 gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc
     3601 ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc
     3661 ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact
     3721 cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg
     3781 cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg
     3841 acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga
     3901 gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca
     3961 ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca
     4021 gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat
     4081 atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga
     4141 attcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg
     4201 tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttgattc
     4261 ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt
     4321 aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca
     4381 gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt
     4441 tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata
     4501 cgactcacta taggctagcc accatgactt cgaaagttta tgatccagaa caaaggaaac
     4561 ggatga
//
