LOCUS       Exported                4585 bp ds-DNA    circular SYN 19-SEP-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pIS1 mIL6R
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 19, 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1461..1580
                     /note="SV40 poly(A) site"
     CDS             1823..2680
                     /codon_start=1
                     /note="open reading frame#3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    4052..4268
                     /note="TK Promoter"
                     /note="TK promoter
                     "
     CDS             4543..5475
                     /codon_start=1
                     /note="open reading frame#2"
                     /translation="PPPGGEGGG?GAGGGGGGGGGGGGGGGGGGREGGGGGEGGGEPGG
                     GGGGGGGRGGGGGGGGGG?GGGGGG?GGGGGGGPGGGGGPGGG?GGGGGGEGGGGGGGG
                     GGGGGGGGGEGGGGGGRGGGGGGGGGGGGGGGGGRGGG?GGEGGGGGGGGGGGGGGGGG
                     EGG?GRGSEGGRGGG??G?REPE?AR??E??GER?G?PE???A??PEGA?G?ERG?ERE
                     GVR?R?PG????R?R?R???G?RE?EPERE?GRAGE?ER???GA?EGE??P?P?G??E?
                     PP?PP???GRAPAPPP?G?PV?PPARGPAP"
ORIGIN
        1 taactggtcc gcagtggtgg gccagatgta aacaaatgaa tgttcttgat tcatttatta
       61 attattatga ttcagaaaaa catgcagaaa atgctgttat ttttttacat ggtaacgcgg
      121 cctcttctta tttatggcga catgttgtgc cacatattga gccagtagcg cggtgtatta
      181 taccagacct tattggtatg ggcaaatcag gcaaatctgg taatggttct tataggttac
      241 ttgatcatta caaatatctt actgcatggt ttgaacttct taatttacca aagaagatca
      301 tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca ttatagctat gagcatcaag
      361 ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa tcatgggatg
      421 aatggcctga tattgaagaa gatattgcgt tgatcaaatc tgaagaagga gaaaaaatgg
      481 ttttggagaa taacttcttc gtggaaacca tgttgccatc aaaaatcatg agaaagttag
      541 aaccagaaga atttgcagca tatcttgaac cattcaaaga gaaaggtgaa gttcgtcgtc
      601 caacattatc atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct gacgttgtac
      661 aaattgttag gaattataat gcttatctac gtgcaagtga tgatttacca aaaatgttta
      721 ttgaatcgga cccaggattc ttttccaatg ctattgttga aggtgccaag aagtttccta
      781 atactgaatt tgtcaaagta aaaggtcttc atttttcgca agaagatgca cctgatgaaa
      841 tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa taattctagg
      901 agctctatac cggtctcgat atcactacta gtgttctaga gtgtctcaat gcacggggca
      961 tttctacctg tgtttctgca gcacccccac tgccttgagt ccccagcagt gctgttattt
     1021 gcctaacacc tgtagccatc tgccacgcag ccagacgtga aacgctgaga cagagaccat
     1081 ttaggttaaa tacgacagct tatcctgctg ggtggggaaa gtaaaaaata tgctggttca
     1141 aggcctaaag taaaatgatc aataatgttt gtaacgtaaa tgaaatattt tcaagaaatg
     1201 tgtccagggg tagcactggc tatgttgacg aggcctttgg taactcagag agctcttggc
     1261 cctgatgggg acttgccctt acgctttctt tatcaggctc tgagttcaca cggagcctct
     1321 ggcacttccc tgctgtcttg ggagaaagga aactggttgc cgcggcaggt tgtggaatct
     1381 gttgctggaa ccaggctgga agcccacctg gtagtgaaca gggcccagtg gggcaggctg
     1441 gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     1501 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     1561 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     1621 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     1681 cgataaggat ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1741 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1801 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1861 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1921 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1981 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     2041 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     2101 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     2161 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     2221 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     2281 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     2341 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     2401 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2461 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     2521 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2581 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2641 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2701 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2761 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2821 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2881 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2941 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3001 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3061 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3121 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3181 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3241 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3301 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3361 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3421 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3481 ttgctggcct tttgctcaca tggctcgaca gatctaaatg agtcttcgga cctcgcgggg
     3541 gccgcttaag cggtggttag ggtttgtctg acgcgggggg agggggaagg aacgaaacac
     3601 tctcattcgg aggcggctcg gggtttggtc ttggtggcca cgggcacgca gaagagcgcc
     3661 gcgatcctct taagcacccc cccgccctcc gtggaggcgg gggtttggtc ggcgggtggt
     3721 aactggcggg ccgctgactc gggcgggtcg cgcgccccag agtgtgacct tttcggtctg
     3781 ctcgcagacc cccgggcggc gccgccgcgg cggcgacggg ctcgctgggt cctaggctcc
     3841 atggggaccg tatacgtgga caggctctgg agcatccgca cgactgcggt gatattaccg
     3901 gagaccttct gcgggacgag ccgggtcacg cggctgacgc ggagcgtccg ttgggcgaca
     3961 aacaccagga cggggcacag gtacactatc ttgtcacccg gaggcgcgag ggactgcagg
     4021 agcttcaggg agtggcgcag ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg
     4081 gtgtccccgg aagaaatata tttgcatgtc tttagttcta tgatgacaca aaccccgccc
     4141 agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt cggggcggcg cggtcccagg
     4201 tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac
     4261 ccgcttaaaa gcttgattct tctgacacaa cagtctcgaa cttaagctgc agaagttggt
     4321 cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga
     4381 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
     4441 ctgacatcca ctttgccttt ctctccacag gtgtccactc ccagttcaat tacagctctt
     4501 aaggctagag tacttaatac gactcactat aggctagcca ccatgacttc gaaagtttat
     4561 gatccagaac aaaggaaacg gatga
//
