LOCUS       Exported                4589 bp ds-DNA    circular SYN 19-SEP-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pIS1 INSIG2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 19, 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1465..1584
                     /note="SV40 poly(A) site"
     CDS             1827..2684
                     /codon_start=1
                     /note="open reading frame#3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    4056..4272
                     /note="TK Promoter"
                     /note="TK promoter
                     "
     CDS             4547..5479
                     /codon_start=1
                     /note="open reading frame#2"
                     /translation="P?PGGEGEGEG?GGGGGGGGGGGGGGGGGGREGGGGGEGGGE?GG
                     GGGGGGGRGGGGGGGGGGAGGGGGG?GGGGGGGGGGGGG?GGG?GGGGGGEGGGGGGGG
                     GGGGGGGGGEGGGGGGRGGGGGGGGGGGGGGGGGRGGG?GGEGGGGGGGGGGGGGGGGG
                     EGGRGRGSEGGRGGG??GARG?GPAR??ES?GERGGEPERA???PP?G?RG?ERG?ERE
                     GPR?R?PG?P??RARER?P?G?REREPEREPGR?G??ER??EGAREGE?EPAP?PEREP
                     GPERPRP??RPRAP?P?????PPG?RGP?P"
ORIGIN
        1 taactggtcc gcagtggtgg gccagatgta aacaaatgaa tgttcttgat tcatttatta
       61 attattatga ttcagaaaaa catgcagaaa atgctgttat ttttttacat ggtaacgcgg
      121 cctcttctta tttatggcga catgttgtgc cacatattga gccagtagcg cggtgtatta
      181 taccagacct tattggtatg ggcaaatcag gcaaatctgg taatggttct tataggttac
      241 ttgatcatta caaatatctt actgcatggt ttgaacttct taatttacca aagaagatca
      301 tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca ttatagctat gagcatcaag
      361 ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa tcatgggatg
      421 aatggcctga tattgaagaa gatattgcgt tgatcaaatc tgaagaagga gaaaaaatgg
      481 ttttggagaa taacttcttc gtggaaacca tgttgccatc aaaaatcatg agaaagttag
      541 aaccagaaga atttgcagca tatcttgaac cattcaaaga gaaaggtgaa gttcgtcgtc
      601 caacattatc atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct gacgttgtac
      661 aaattgttag gaattataat gcttatctac gtgcaagtga tgatttacca aaaatgttta
      721 ttgaatcgga cccaggattc ttttccaatg ctattgttga aggtgccaag aagtttccta
      781 atactgaatt tgtcaaagta aaaggtcttc atttttcgca agaagatgca cctgatgaaa
      841 tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa taattctagg
      901 agctctatac cggtctcgat atcactacta gtgttctaga gtattttctc tttagcttac
      961 tgttgtgcct ttttattttt ctaatcacag cagtatgagt tatgagtgcc ctaatttgtg
     1021 gttagtttct aatttaatgt tgtttcatag agtttggagt gttttgatac agggtgaaaa
     1081 tgaacttctg gtttcaaacc tgcgttactg gagacagccc aaagagtaat tttctgtttt
     1141 gacaggtttt actggaagta tatgtgatga gcagaagagg ttatcagcat taaattgttt
     1201 tggttctaaa tttggaacag tatatataat taaaagtaag gaacattaga ggatttaatt
     1261 agaataaata catgttttgg aaatacagtg acctcttgca gtgtcacaaa agtgcaaagt
     1321 gatattagct gtcatctgca atacagaatc tcattgcttt tgcacatgga gcatatagga
     1381 aactccaaac agatcacaat gaggtttcta aatctgttgg gttctgtctt ctattgggtt
     1441 ctgtgcggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     1501 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     1561 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     1621 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     1681 aaatcgataa ggatccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     1741 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1801 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     1861 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1921 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1981 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2041 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     2101 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2161 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2221 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2281 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2341 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     2401 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2461 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2521 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     2581 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2641 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     2701 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     2761 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2821 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2881 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2941 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3001 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3061 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3121 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3181 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3241 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3301 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3361 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3421 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3481 ccttttgctg gccttttgct cacatggctc gacagatcta aatgagtctt cggacctcgc
     3541 gggggccgct taagcggtgg ttagggtttg tctgacgcgg ggggaggggg aaggaacgaa
     3601 acactctcat tcggaggcgg ctcggggttt ggtcttggtg gccacgggca cgcagaagag
     3661 cgccgcgatc ctcttaagca cccccccgcc ctccgtggag gcgggggttt ggtcggcggg
     3721 tggtaactgg cgggccgctg actcgggcgg gtcgcgcgcc ccagagtgtg accttttcgg
     3781 tctgctcgca gacccccggg cggcgccgcc gcggcggcga cgggctcgct gggtcctagg
     3841 ctccatgggg accgtatacg tggacaggct ctggagcatc cgcacgactg cggtgatatt
     3901 accggagacc ttctgcggga cgagccgggt cacgcggctg acgcggagcg tccgttgggc
     3961 gacaaacacc aggacggggc acaggtacac tatcttgtca cccggaggcg cgagggactg
     4021 caggagcttc agggagtggc gcagctgctt catccccgtg gcccgttgct cgcgtttgct
     4081 ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt tctatgatga cacaaacccc
     4141 gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc
     4201 caggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag
     4261 cgacccgctt aaaagcttga ttcttctgac acaacagtct cgaacttaag ctgcagaagt
     4321 tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta aggagaccaa
     4381 tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc acctattggt
     4441 cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccagtt caattacagc
     4501 tcttaaggct agagtactta atacgactca ctataggcta gccaccatga cttcgaaagt
     4561 ttatgatcca gaacaaagga aacggatga
//
