LOCUS       Exported                4475 bp ds-DNA    circular SYN 19-SEP-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pIS1 mPEL0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 19, 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1351..1470
                     /note="SV40 poly(A) site"
     CDS             1713..2570
                     /codon_start=1
                     /note="open reading frame#3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    3942..4158
                     /note="TK Promoter"
                     /note="TK promoter
                     "
     CDS             4433..5365
                     /codon_start=1
                     /note="open reading frame#2"
                     /translation="PPPGGEGGGEG?GGGGGGGGGGGGGGGGGGREGGGGGEGGGE?GG
                     GGGGGGGRGGGGGGGGGGAGGGGGGPGGGGGGGPGGGGG?GGG?GGGGGGEGGGGGGGG
                     GGGGGGGGGEGGGGGGRGGGGGGGGGGGGGGGGGRGGG?GGEGGGGRGGGGGGGGGGGG
                     EGGRGRGSEGGRGGG??G?RG?GPAR??E??GERGGE?E?????PP?G?RG?ERG?ERE
                     GPR?R?PGR??GR?RER?G?G?RE??PEREPGR?RE?ER???GAREGE?EPAP?PER?P
                     GPERPRP?PRPPAPGP???P??PP?RPP?P"
ORIGIN
        1 taactggtcc gcagtggtgg gccagatgta aacaaatgaa tgttcttgat tcatttatta
       61 attattatga ttcagaaaaa catgcagaaa atgctgttat ttttttacat ggtaacgcgg
      121 cctcttctta tttatggcga catgttgtgc cacatattga gccagtagcg cggtgtatta
      181 taccagacct tattggtatg ggcaaatcag gcaaatctgg taatggttct tataggttac
      241 ttgatcatta caaatatctt actgcatggt ttgaacttct taatttacca aagaagatca
      301 tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca ttatagctat gagcatcaag
      361 ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa tcatgggatg
      421 aatggcctga tattgaagaa gatattgcgt tgatcaaatc tgaagaagga gaaaaaatgg
      481 ttttggagaa taacttcttc gtggaaacca tgttgccatc aaaaatcatg agaaagttag
      541 aaccagaaga atttgcagca tatcttgaac cattcaaaga gaaaggtgaa gttcgtcgtc
      601 caacattatc atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct gacgttgtac
      661 aaattgttag gaattataat gcttatctac gtgcaagtga tgatttacca aaaatgttta
      721 ttgaatcgga cccaggattc ttttccaatg ctattgttga aggtgccaag aagtttccta
      781 atactgaatt tgtcaaagta aaaggtcttc atttttcgca agaagatgca cctgatgaaa
      841 tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa taattctagg
      901 agctctatac cggtctcgat atcactacta gtgttctaga cacactgcat gctttgaaac
      961 agttttcctt agaaaaagct tttgctatct tatcctgttt acattatttc tttattttaa
     1021 ttctgcttgg tgttcttgca ttgcatttaa tgatcccttt tctccccacc tccacacact
     1081 acattttttt tagatttaaa tagttttact attttaaatg attgccgtac aattagtaga
     1141 cttgaagaca agttttaaat atttttcttc aaaggcttgt taaaccaatc atgttaaaag
     1201 gaaattcttg gttttggttt gttgttgtta acgtaagtca tatttgattt agagggtaac
     1261 ttaaatcagt tatttttagc tttttagaac tttgatctgc tagggattgt caaaataatc
     1321 tccttgaggc gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     1381 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     1441 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     1501 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     1561 gtggtaaaat cgataaggat ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1621 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1681 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1741 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1801 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1861 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     1921 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     1981 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2041 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2101 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2161 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2221 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     2281 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2341 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2401 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2461 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2521 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2581 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2641 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2701 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2761 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2821 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2881 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2941 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3001 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3061 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3121 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3181 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3241 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3301 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3361 tcctggcctt ttgctggcct tttgctcaca tggctcgaca gatctaaatg agtcttcgga
     3421 cctcgcgggg gccgcttaag cggtggttag ggtttgtctg acgcgggggg agggggaagg
     3481 aacgaaacac tctcattcgg aggcggctcg gggtttggtc ttggtggcca cgggcacgca
     3541 gaagagcgcc gcgatcctct taagcacccc cccgccctcc gtggaggcgg gggtttggtc
     3601 ggcgggtggt aactggcggg ccgctgactc gggcgggtcg cgcgccccag agtgtgacct
     3661 tttcggtctg ctcgcagacc cccgggcggc gccgccgcgg cggcgacggg ctcgctgggt
     3721 cctaggctcc atggggaccg tatacgtgga caggctctgg agcatccgca cgactgcggt
     3781 gatattaccg gagaccttct gcgggacgag ccgggtcacg cggctgacgc ggagcgtccg
     3841 ttgggcgaca aacaccagga cggggcacag gtacactatc ttgtcacccg gaggcgcgag
     3901 ggactgcagg agcttcaggg agtggcgcag ctgcttcatc cccgtggccc gttgctcgcg
     3961 tttgctggcg gtgtccccgg aagaaatata tttgcatgtc tttagttcta tgatgacaca
     4021 aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt cggggcggcg
     4081 cggtcccagg tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc
     4141 ctgcagcgac ccgcttaaaa gcttgattct tctgacacaa cagtctcgaa cttaagctgc
     4201 agaagttggt cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga
     4261 gaccaataga aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct
     4321 attggtctta ctgacatcca ctttgccttt ctctccacag gtgtccactc ccagttcaat
     4381 tacagctctt aaggctagag tacttaatac gactcactat aggctagcca ccatgacttc
     4441 gaaagtttat gatccagaac aaaggaaacg gatga
//
