LOCUS       Exported               13895 bp ds-DNA     circular SYN 24-SEP-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSECC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13895)
  AUTHORS   FSR
  TITLE     Direct Submission
  JOURNAL   Exported Sep 24, 2018 from SnapGene 4.2.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13895
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        237..616
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1685..1918
                     /label=RRE
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2445..2562
                     /label=cPPT/CTS
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2625..2654
                     /label=pAF Gibson
                     /note="pAF Gibson"
     promoter        2655..2895
                     /note="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2900..4784
                     /label=Filler
                     /note="Filler"
     misc_RNA        4785..4860
                     /label=gRNA scaffold
                     /note="gRNA scaffold"
                     /note="guide RNA scaffold for the CRISPR/Cas9 system"
     misc_feature    4908..5025
                     /label=cPPT/CTS
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5069..5098
                     /label=pBF Gibson
                     /note="pBF Gibson"
     promoter        5124..5335
                     /note="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    5350..5379
                     /label=gBF Gibson
                     /note="gBF Gibson"
     CDS             5383..5406
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG(R) epitope tag, followed by an enterokinas
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             5413..5433
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=nuclear localization signal of SV40 large T ant
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             5458..9558
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9 (Csn1) endonuclease from the Streptococcus
                     /note="Cas9"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9559..9606
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=bipartite nuclear localization signal from nucl
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9622..9678
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=2A peptide from porcine teschovirus-1 polyprotein
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9682..9702
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=nuclear localization signal of SV40 large T ant
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             9703..10731
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=site-specific recombinase
                     /note="Cre"
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    10732..10761
                     /label=gBR Gibson
                     /note="gBR Gibson"
     misc_feature    10784..11372
                     /label=WPRE
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(11255..11266)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa recognition and cleavage site
                     /note="Factor Xa site"
                     /translation="IEGR"
     primer_bind     complement(11375..11391)
                     /label=KS primer
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             11901..12081
                     /label=5' LTR (truncated)
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(12143..12731)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12902..13762)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13763..13867)
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag
     1561 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1621 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1681 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1741 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1801 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1861 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1921 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1981 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     2041 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     2101 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2161 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2221 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2281 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2341 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     2401 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2461 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2521 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2581 agcagagatc cagtttggtt agtaccgggc ccgggtcgac tagtgatcag tgtgagggag
     2641 tgtaaagctg gtttgagggc ctatttccca tgattccttc atatttgcat atacgataca
     2701 aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa
     2761 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     2821 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     2881 tcttgtggaa aggacgaaac accggagacg gttgtaaatg agcacacaaa atacacatgc
     2941 taaaatatta tattctatga cctttataaa atcaaccaaa atcttctttt taataacttt
     3001 agtatcaata attagaattt ttatgttcct ttttgcaaac ttttaataaa aatgagcaaa
     3061 ataaaaaaac gctagtttta gtaactcgcg ttgttttctt cacctttaat aatagctact
     3121 ccaccacttg ttcctaagcg gtcagctcct gcttcaatca ttttttgagc atcttcaaat
     3181 gttctaactc caccagctgc tttaactaaa gcattgtctt taacaactga cttcattagt
     3241 ttaacatctt caaatgttgc acctgatttt gaaaatcctg ttgatgtttt aacaaattct
     3301 aatccagctt caacagctat ttcacaagct ttcatgattt cttcttttgt taataaacaa
     3361 ttttccataa tacatttaac aacatgtgat ccagctgctt tttttacagc tttcatgtct
     3421 tctaaaacta attcataatt tttgtctttt aatgcaccaa tatttaatac catatcaatt
     3481 tctgttgcac catctttaat tgcttcagaa acttcgaatg cttttgtagc tgttgtgcat
     3541 gcacctagag gaaaacctac aacatttgtt attcctacat ttgtgccttt taataattct
     3601 ttacaatagc ttgttcaata tgaattaaca caaactgttg caaaatcaaa ttcaattgct
     3661 tcatcacata attgtttaat ttcagctttc gtagcatctt gttttaataa tgtgtgatct
     3721 atatatttgt ttagtttcat tttttctcct atatattcat ttttaatttt aattctttaa
     3781 taatttcgtc tactttaact ttagcgtttt gaacagattc accaacacct ataaaataaa
     3841 tttttagttt aggttcagtt ccacttgggc gaacagcaaa tcatgactta tcttctaaat
     3901 aaaattttag taagtcttgt cctggcatat tatacattcc atcgatgtag tcttcaacat
     3961 taacaacttt aagtccagca atttgagtta agggtgttgc tctcaatgat ttcattaatg
     4021 gttcaatttt taatttcttt tcttctggtt taaaattcaa gtttaaagtg aaagtgtaat
     4081 atgcacccat ttctttaaat aaatcttcta aatagtctac taatgtttta ttttgttttt
     4141 tataaaatca agcagcctct gctattaata tagaagcttg tattccatct ttatctctag
     4201 ctgagtcatc aattacatat ccataacttt cttcataagc aaaaacaaaa tttaatccgt
     4261 tatcttcttc tttagcaatt tctctaccca ttcatttaaa tccagttaaa gtttttacaa
     4321 tattaactcc atatttttca tgagcgattc tatcacccaa atcacttgtt acaaaacttg
     4381 aatatagagc cggatttttt ggaatgctat ttaagcgttt tagatttgat aattttcaat
     4441 caattaaaat tggtcctgtt tgatttccat ctaatcttac aaaatgacca tcatgtttta
     4501 ttgccattcc aaatctgtca gcatctgggt cattcataat aataatatct gcatcatgtt
     4561 taataccata ttcaagcggt atttttcatg caggatcaaa ttctggattt ggatttacaa
     4621 catttttaaa tgtttcatct tcaaatgcat gctcttcaac ctcaataacg ttatatcctg
     4681 attcacgtaa tatttttggg gtaaatttag ttcctgttcc attaactgcg ctaaaaataa
     4741 tttttaaatc ttttttagct tcttgctctt ttttgtacgt ctctgtttta gagctagaaa
     4801 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     4861 ttttttgaat tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
     4921 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     4981 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     5041 gacagcagag atccactttg gcgccggcaa accgctgttc ctaggaatcc cgaggccttc
     5101 gagtggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     5161 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     5221 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     5281 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtgtc
     5341 gtgacgcggg acccgacatt agcgctacag cttaagcgga tggattacaa agacgatgac
     5401 gataagatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgac
     5461 aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc
     5521 gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc
     5581 atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc
     5641 cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg
     5701 caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa
     5761 gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc
     5821 gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg
     5881 gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc
     5941 aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac
     6001 aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac
     6061 gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg
     6121 gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt
     6181 gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc
     6241 aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc
     6301 ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg
     6361 agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc
     6421 aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag
     6481 ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac
     6541 attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag
     6601 atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag
     6661 cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt
     6721 ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag
     6781 atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc
     6841 gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg
     6901 gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg
     6961 cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac
     7021 gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc
     7081 gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag
     7141 cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc
     7201 gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag
     7261 gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc
     7321 ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg
     7381 ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg
     7441 agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc
     7501 ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg
     7561 acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag
     7621 cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag
     7681 gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa
     7741 atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag
     7801 cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa
     7861 aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg
     7921 tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg
     7981 cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag
     8041 aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac
     8101 tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag
     8161 gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg
     8221 gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag
     8281 tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg
     8341 gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac
     8401 cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct
     8461 aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc
     8521 gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc
     8581 atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg
     8641 atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc
     8701 gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca
     8761 ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga
     8821 aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct
     8881 gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag
     8941 ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg
     9001 gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc
     9061 ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag
     9121 ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat
     9181 gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac
     9241 aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg
     9301 gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc
     9361 agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc
     9421 gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg
     9481 ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg
     9541 tctcagctgg gaggcgacaa gcgtcctgct gctactaaga aagctggtca agctaagaaa
     9601 aagaaagcta gcggcagcgg cgccaccaac ttcagcctgc tgaagcaggc cggcgacgtg
     9661 gaggagaacc ccggccccat gcccaagaag aagaggaagg tgtccaattt actgaccgta
     9721 caccaaaatt tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg
     9781 atggacatgt tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc
     9841 gtttgccggt cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa
     9901 cctgaagatg ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact
     9961 atccagcaac atttgggcca gctaaacatg cttcatcgtc ggtccgggct gccacgacca
    10021 agtgacagca atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc
    10081 ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca
    10141 ctcatggaaa atagcgatcg ctgccaggat atacgtaatc tggcatttct ggggattgct
    10201 tataacaccc tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt
    10261 actgacggtg ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca
    10321 ggtgtagaga aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgta
    10381 tctggtgtag ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt
    10441 gccgcgccat ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca
    10501 actcatcgat tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct
    10561 ggacacagtg cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg
    10621 gagatcatgc aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac
    10681 ctggatagtg aaacaggggc aatggtgcgc ctgctggaag atggcgatta gaaacgttgt
    10741 tgtttggggt tgaattactc ttctagaccg cggcgcgccc gataatcaac ctctggatta
    10801 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
    10861 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
    10921 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
    10981 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
    11041 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
    11101 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
    11161 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
    11221 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
    11281 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
    11341 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc
    11401 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
    11461 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
    11521 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
    11581 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
    11641 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
    11701 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
    11761 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
    11821 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
    11881 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    11941 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    12001 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    12061 cagtgtggaa aatctctagc agcatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    12121 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    12181 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    12241 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    12301 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    12361 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    12421 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    12481 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    12541 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    12601 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    12661 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    12721 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    12781 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    12841 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    12901 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    12961 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    13021 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    13081 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    13141 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    13201 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    13261 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    13321 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    13381 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    13441 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    13501 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    13561 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    13621 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    13681 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    13741 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    13801 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    13861 ttccgcgcac atttccccga aaagtgccac ctgac
//
