LOCUS       pMSCV-Luciferase        8003 bp    DNA     circular      7-OCT-2009
DEFINITION  MSCV-IRES-GFP     6471 bp.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|531510510|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMSCV-Luciferase-IRES-mNras(G12D)@11|
COMMENT     VNTAUTHORNAME|Johannes Zuber|
COMMENT     VNTAUTHOREML|zuber@imp.ac.at|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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FEATURES             Location/Qualifiers
     enhancer        3154..3723
                     /vntifkey="9"
                     /label=IRES
     LTR             1..515
                     /vntifkey="19"
                     /label=5'LTR
     LTR             4395..4878
                     /vntifkey="19"
                     /label=3'LTR
     rep_origin      5449..5449
                     /vntifkey="33"
                     /label=OoR
     CDS             6209..7069
                     /vntifkey="4"
                     /label=Amp
     CDS             3730..4299
                     /vntifkey="4"
                     /label=mNras(G12D)
     misc_feature    516..1404
                     /vntifkey="21"
                     /label=Psi+
     CDS             1419..3071
                     /vntifkey="4"
                     /label=Luci
     primer          3772..4253
                     /vntifkey="27"
                     /label=seq.IF
BASE COUNT     2012 a      2017 c      2027 g      1947 t 
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata 
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca 
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga 
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg 
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt 
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 
     1381 ccctcactcc ttctctaggc gccggaatta gatccaccat ggaagacgcc aaaaacataa 
     1441 agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata 
     1501 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg 
     1561 aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga 
     1621 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat 
     1681 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca 
     1741 tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg 
     1801 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa 
     1861 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg 
     1921 tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata 
     1981 gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg 
     2041 tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg 
     2101 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg 
     2161 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat 
     2221 ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg 
     2281 tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta 
     2341 atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg 
     2401 ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag 
     2461 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat 
     2521 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag 
     2581 gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga 
     2641 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg 
     2701 aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc 
     2761 aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag 
     2821 gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg 
     2881 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa 
     2941 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg 
     3001 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga 
     3061 tcgccgtgta attctaggat ccgtcgacct gcagccatgc attgattcaa aattttccga 
     3121 tcacgagact agcctcgagg ttaacgaatt ccgccccccc cccctaacgt tactggccga 
     3181 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg 
     3241 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg 
     3301 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt 
     3361 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac 
     3421 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca 
     3481 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg 
     3541 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg 
     3601 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac 
     3661 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg 
     3721 gccacaacca tgactgagta caaactggtg gtggttggag cagatggtgt tgggaaaagc 
     3781 gccttgacga tccagctaat ccagaaccac tttgtggatg aatatgatcc caccatagag 
     3841 gattcttacc gaaagcaagt ggtgattgat ggtgagacct gcctgctgga catactggac 
     3901 acagctggac aagaggagta cagtgccatg agagaccagt acatgaggac aggcgaaggg 
     3961 ttcctctgtg tatttgccat caataatagc aaatcatttg cagatattaa cctctacagg 
     4021 gagcaaatta agcgtgtgaa agattctgat gatgtcccca tggtgctggt aggcaacaag 
     4081 tgtgacttgc caacaaggac agttgacaca aagcaagccc acgaactggc caagagttac 
     4141 ggaattccat tcattgagac ctcagccaag acccgacagg gtgtggagga tgccttttac 
     4201 acactggtaa gggagatacg ccagtaccga atgaaaaagc tcaacagcag tgacgatggc 
     4261 actcaaggtt gtatggggct gccctgtgtg ctgatgtagt cgacctgcag ccaagcttat 
     4321 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct 
     4381 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa atacataact 
     4441 gagaatagag aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac 
     4501 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag 
     4561 atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag 
     4621 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt 
     4681 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc 
     4741 agtcctccga tagactgcgt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt 
     4801 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc 
     4861 gtcagcgggg gtctttcatg ggtaacagtt tcttgaagtt ggagaacaac attctgaggg 
     4921 taggagtcga atattaagta atcctgactc aattagccac tgttttgaat ccacatactc 
     4981 caatactcct gaaatagttc attatggaca gcgcagaaga gctggggaga attaattcgt 
     5041 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 
     5101 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 
     5161 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 
     5221 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 
     5281 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
     5341 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
     5401 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
     5461 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
     5521 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
     5581 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
     5641 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
     5701 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
     5761 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
     5821 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
     5881 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
     5941 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
     6001 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
     6061 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 
     6121 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 
     6181 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 
     6241 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 
     6301 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 
     6361 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 
     6421 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 
     6481 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 
     6541 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 
     6601 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 
     6661 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 
     6721 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 
     6781 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 
     6841 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 
     6901 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 
     6961 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 
     7021 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 
     7081 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 
     7141 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 
     7201 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 
     7261 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 
     7321 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 
     7381 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac 
     7441 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 
     7501 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 
     7561 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 
     7621 cgccagggtt ttcccagtca cgacgttgta aaacgacggc gcaaggaatg gtgcatgcaa 
     7681 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca 
     7741 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata 
     7801 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag 
     7861 gcgattagtc caatttgtta aagacaggat atcagtggtc caggctctag ttttgactca 
     7921 acaatatcac cagctgaagc ctatagagta cgagccatag ataaaataaa agattttatt 
     7981 tagtctccag aaaaaggggg gaa 
//
