LOCUS pSLQ1371_with_Blp1 8888 bp ds-DNA circular 20-OCT-2014 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers CDS complement(7898..8557) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=AmpR /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 rep_origin complement(7118..7800) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_recomb 2825..2858 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=LoxP recombination site /ApEinfo_fwdcolor=steel blue /ApEinfo_revcolor=orange misc_recomb 5688..5721 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=LoxP recombination site(1) /ApEinfo_label=LoxP recombination site /ApEinfo_fwdcolor=steel blue /ApEinfo_revcolor=orange misc_feature complement(8797..8825) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=AmpR promoter /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 misc_feature 844..1024 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=HIV-1_5_LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 4970..5665 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=BFP /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green misc_feature 6894..7074 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=HIV-1_5_LTR(1) /ApEinfo_label=HIV-1_5_LTR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 5767..6367 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=wPRE /ApEinfo_fwdcolor=#0060ff /ApEinfo_revcolor=#0060ff misc_feature 4301..4957 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=puro-t2A /ApEinfo_fwdcolor=#ca72ff /ApEinfo_revcolor=#ca72ff misc_feature 2945..2964 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=EGFP_NT2 /ApEinfo_fwdcolor=#ffa1e9 /ApEinfo_revcolor=#ffa1e9 misc_feature complement(1601..1607) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=TATA box /ApEinfo_fwdcolor=#ff9afa /ApEinfo_revcolor=#ff9afa misc_feature complement(766..775) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=GC Box /ApEinfo_fwdcolor=#888eff /ApEinfo_revcolor=#888eff misc_feature complement(7145..7154) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=GC Box(1) /ApEinfo_label=GC Box /ApEinfo_fwdcolor=#888eff /ApEinfo_revcolor=#888eff misc_feature 2631..2944 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=mU6 promoter (modified) /ApEinfo_fwdcolor=#fff431 /ApEinfo_revcolor=#fff431 misc_feature 2965..2976 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=modified stem loop /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 2978..2989 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=modified stem loop(1) /ApEinfo_label=modified stem loop /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 2991..3057 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=modified stem loop(2) /ApEinfo_label=modified stem loop /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 3115..4076 /label=EF1Alpha /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 4078..4232 /label=EF1Alpha(1) /ApEinfo_label=EF1Alpha /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 4895..4969 /label=T2A /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green ORIGIN 1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa 61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg 121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca 181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata 241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct 1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa 1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa 1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg 1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag 1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc 1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa 1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg 1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag 2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa 2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg 2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga 2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag 2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc 2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa 2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc 2641 gccatctcta ggcccgcgcc ggccccctcg cacggacttg tgggagaagc tcggctactc 2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga 2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat 2821 ttctataact tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca 2881 ccctaactgt aaagtaattg tgtgttttga gactataaGt atcccttgga gaaCCAcctT 2941 GTTGGACCAG GATGGGCACC ACCCGTTTAA GAGCTAAGCT GGAAACAGCA TAGCAAGTTT 3001 AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC GAGTCGGTGC TTTTTTTCtc 3061 gagtactagg atccattagg cggccgcgtg gataaccgta ttaccgccat gcatGTGCCC 3121 GTCAGTGGGC AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA 3181 ATTGAACCGG TGCCTAGAGA AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT 3241 GGCTCCGCCT TTTTCCCGAG GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA 3301 ACGTTCTTTT TCGCAACGGG TTTGCCGCCA GAACACAGGT AAGTGCCGTG TGTGGTTCCC 3361 GCGGGCCTGG CCTCTTTACG GGTTATGGCC CTTGCGTGCC TTGAATTACT TCCACCTGGC 3421 TGCAGTACGT GATTCTTGAT CCCGAGCTTC GGGTTGGAAG TGGGTGGGAG AGTTCGAGGC 3481 CTTGCGCTTA AGGAGCCCCT TCGCCTCGTG CTTGAGTTGA GGCCTGGCCT GGGCGCTGGG 3541 GCCGCCGCGT GCGAATCTGG TGGCACCTTC GCGCCTGTCT CGCTGCTTTC GATAAGTCTC 3601 TAGCCATTTA AAATTTTTGA TGACCTGCTG CGACGCTTTT TTTCTGGCAA GATAGTCTTG 3661 TAAATGCGGG CCAAGATCTG CACACTGGTA TTTCGGTTTT TGGGGCCGCG GGCGGCGACG 3721 GGGCCCGTGC GTCCCAGCGC ACATGTTCGG CGAGGCGGGG CCTGCGAGCG CGGCCACCGA 3781 GAATCGGACG GGGGTAGTCT CAAGCTGGCC GGCCTGCTCT GGTGCCTGGC CTCGCGCCGC 3841 CGTGTATCGC CCCGCCCTGG GCGGCAAGGC TGGCCCGGTC GGCACCAGTT GCGTGAGCGG 3901 AAAGATGGCC GCTTCCCGGC CCTGCTGCAG GGAGCTCAAA ATGGAGGACG CGGCGCTCGG 3961 GAGAGCGGGC GGGTGAGTCA CCCACACAAA GGAAAAGGGC CTTTCCGTCC TCAGCCGTCG 4021 CTTCATGTGA CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG TTCTCGcGCT 4081 TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG TTTCCCCACA 4141 CTGAGTGGGT GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC TCCTTGGAAT 4201 TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG GTTCAAAGTT 4261 TTTTTCTTCC ATTTCAGGTG TCGTGAgCTA GCCCACCATG ACCGAGTACA AGCCCACGGT 4321 GCGCCTCGCC ACCCGCGACG ACGTCCCCCG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC 4381 CGACTACCCC GCCACGCGCC ACACCGTCGA CCCGGACCGC CACATCGAGC GGGTCACCGA 4441 GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA 4501 CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT 4561 CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA 4621 GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT 4681 CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT 4741 GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT 4801 CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC 4861 GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCGGATCG GGAGAGGGCA GAGGAAGTCT 4921 GCTAACATGC GGTGACGTCG AGGAGAATCC TGGCCCAccg gtcgccaccA GCGAGCTGAT 4981 TAAGGAGAAC ATGCACATGA AGCTGTACAT GGAGGGCACC GTGGACAACC ATCACTTCAA 5041 GTGCACATCC GAGGGCGAAG GCAAGCCCTA CGAGGGCACC CAGACCATGA GAATCAAGGT 5101 GGTCGAGGGC GGCCCTCTCC CCTTCGCCTT CGACATCCTG GCTACTAGCT TCCTCTACGG 5161 CAGCAAGACC TTCATCAACC ACACCCAGGG CATCCCCGAC TTCTTCAAGC AGTCCTTCCC 5221 TGAGGGCTTC ACATGGGAGA GAGTCACCAC ATACGAAGAC GGGGGCGTGC TGACCGCTAC 5281 CCAGGACACC AGCCTCCAGG ACGGCTGCCT CATCTACAAC GTCAAGATCA GAGGGGTGAA 5341 CTTCACATCC AACGGCCCTG TGATGCAGAA GAAAACACTC GGCTGGGAGG CCTTCACCGA 5401 GACGCTGTAC CCCGCTGACG GCGGCCTGGA AGGCAGAAAC GACATGGCCC TGAAGCTCGT 5461 GGGCGGGAGC CATCTGATCG CAAACATCAA GACCACATAT AGATCCAAGA AACCCGCTAA 5521 GAACCTCAAG ATGCCTGGCG TCTACTATGT GGACTACAGA CTGGAAAGAA TCAAGGAGGC 5581 CAACAACGAG ACCTACGTCG AGCAGCACGA GGTGGCAGTG GCCAGATACT GCGACCTCCC 5641 TAGCAAACTG GGGCACAAGC TTAATtaaga attcgtcgag ggacctaata acttcgtata 5701 gcatacatta tacgaagtta tacatgttta agggttccgg ttccactagg tacaattcga 5761 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 5821 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 5881 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 5941 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 6001 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 6061 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 6121 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc 6181 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 6241 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 6301 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc 6361 cccgcatcga taccgtcgac ctcgatcgag acctagaaaa acatggagca atcacaagta 6421 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg 6481 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag 6541 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa 6601 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga 6661 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag 6721 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac 6781 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg 6841 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc 6901 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 6961 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 7021 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatgt 7081 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 7141 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 7201 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 7261 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 7321 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 7381 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 7441 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 7501 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 7561 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 7621 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 7681 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 7741 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 7801 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 7861 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 7921 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 7981 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 8041 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 8101 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 8161 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 8221 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 8281 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 8341 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 8401 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 8461 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 8521 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 8581 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 8641 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 8701 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 8761 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 8821 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 8881 cacctgac //