LOCUS       636\pBAD-I-SceI         5238 bp    DNA     circular     14-JUL-2005
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|329831316|
COMMENT     VNTDBDATE|380041133|
COMMENT     LSOWNER|
COMMENT     VNTNAME|636 pBAD-I-SceI|
COMMENT     VNTAUTHORNAME|Benedikt Kaufer|
COMMENT     VNTUDF|Antibiotic|5|none|
COMMENT     VNTUDF|Freezer Position|1|636|
FEATURES             Location/Qualifiers
     CDS             complement(3285..4163)
                     /vntifkey="4"
                     /label=araC
     misc_feature    4192..4209
                     /vntifkey="21"
                     /label=operator\O2
                     /note="operator O2"
     promoter        complement(4314..4342)
                     /vntifkey="30"
                     /label=araC\promoter
                     /note="araC promoter"
     misc_feature    4350..4371
                     /vntifkey="21"
                     /label=operator\O1
                     /note="operator O1"
     misc_feature    4393..4406
                     /vntifkey="21"
                     /label=CAP\site
                     /note="CAP site"
     misc_feature    4402..4440
                     /vntifkey="21"
                     /label=operator\I2\and\I1
                     /note="operator I2 + I1"
     promoter        4439..4466
                     /vntifkey="30"
                     /label=arabinose\BAD\promoter
                     /note="arabinose pBAD promoter"
     misc_feature    4495..5233
                     /vntifkey="21"
                     /label=I-SceI\fragment
                     /note="Sce fragment - OF131/132 PCR product NheI/PstI restriction fragment"
     gene            4509..5222
                     /vntifkey="60"
                     /note="I.SceI meganuclease"
     gene            14..439
                     /gene="rrnB"
                     /vntifkey="60"
     terminator      14..439
                     /gene="rrnB"
                     /vntifkey="43"
                     /label=rrnB
     promoter        494..500
                     /vntifkey="30"
                     /label=Promoter_P_3
                     /note="bla P3 promoter"
     gene            532..1395
                     /gene="bla"
                     /vntifkey="60"
     mRNA            532..1395
                     /gene="bla"
                     /vntifkey="54"
                     /label=bla
     rep_origin      1431..1889
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="M13 origin"
     rep_origin      1895..2592
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR322 origin"
BASE COUNT     1344 a      1364 c      1264 g      1266 t 
ORIGIN
        1 ggcatgcaag cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa 
       61 atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt 
      121 cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg 
      181 gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga 
      241 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa 
      301 atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac 
      361 gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt 
      421 ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat gtatccgctc 
      481 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 
      541 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 
      601 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 
      661 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 
      721 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac 
      781 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 
      841 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 
      901 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 
      961 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 
     1021 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 
     1081 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 
     1141 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 
     1201 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 
     1261 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 
     1321 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 
     1381 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttacgcgcc 
     1441 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 
     1501 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 
     1561 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 
     1621 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc 
     1681 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 
     1741 gttccaaact tgaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat 
     1801 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 
     1861 ttttaacaaa atattaacgt ttacaattta aaaggatcta ggtgaagatc ctttttgata 
     1921 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 
     1981 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     2041 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     2101 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 
     2161 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     2221 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     2281 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     2341 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 
     2401 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     2461 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     2521 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     2581 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     2641 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 
     2701 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 
     2761 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc 
     2821 gcatagggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc 
     2881 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg 
     2941 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag caaggagatg gcgcccaaca 
     3001 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga 
     3061 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac 
     3121 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgc tcatgtttga 
     3181 cagcttatca tcgatgcata atgtgcctgt caaatggacg aagcagggat tctgcaaacc 
     3241 ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg 
     3301 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg 
     3361 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg 
     3421 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc 
     3481 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac 
     3541 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga 
     3601 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg 
     3661 ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc 
     3721 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc 
     3781 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta 
     3841 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga 
     3901 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc 
     3961 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga 
     4021 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg 
     4081 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg 
     4141 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat 
     4201 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc 
     4261 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca 
     4321 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac 
     4381 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga 
     4441 cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttgggc tagcaggagg 
     4501 gtacctatat gcatatgaaa aacatcaaaa aaaaccaggt aatgaacctg ggtccgaact 
     4561 ctaaactgct gaaagaatac aaatcccagc tgatcgaact gaacatcgaa cagttcgaag 
     4621 caggtatcgg tctgatcctg ggtgatgctt acatccgttc tcgtgatgaa ggtaaaacct 
     4681 actgtatgca gttcgagtgg aaaaacaaag catacatgga ccacgtatgt ctgctgtacg 
     4741 atcagtgggt actgtccccg ccgcacaaaa aagaacgtgt taaccacctg ggtaacctgg 
     4801 taatcacctg gggcgcccag actttcaaac accaagcttt caacaaactg gctaacctgt 
     4861 tcatcgttaa caacaaaaaa accatcccga acaacctggt tgaaaactac ctgaccccga 
     4921 tgtctctggc atactggttc atggatgatg gtggtaaatg ggattacaac aaaaactcta 
     4981 ccaacaaatc gatcgtactg aacacccagt ctttcacttt cgaagaagta gaatacctgg 
     5041 ttaagggtct gcgtaacaaa ttccaactga actgttacgt aaaaatcaac aaaaacaaac 
     5101 cgatcatcta catcgattct atgtcttacc tgatcttcta caacctgatc aaaccgtacc 
     5161 tgatcccgca gatgatgtac aaactgccga acactatctc ctccgaaact ttcctgaaat 
     5221 aagggcccga cgtctgca 
//
