LOCUS       pAE03        9474 bp    DNA   CIRCULAR SYN        31-AUG-2009
DEFINITION  Ligation of Fragment 1 into Fragment 2
ACCESSION   pAE03
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     CDS             complement (2077..2892)
                     /gene="spc"
     CDS             complement (5853..6590)
                     /gene="ery"
     CDS             7810..8517
                     /gene="gfp'"
     misc_feature    complement (8543..8631)
                     /gene="(null)"
                     /product="ter"
     misc_feature    complement (8638..8724)
                     /gene="ter"
BASE COUNT     2578 a   2087 c   1968 g   2841 t
ORIGIN      
        1 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
       61 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      421 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
      481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
      541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
      601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
      661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
      721 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
      781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
      841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
      901 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
      961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc tatcaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcccg acctcgagct
     1861 ggatacttcc cgtccgccag ggggacatgc cggcgatgct gaaggtcgcg cgcattcccg
     1921 atgaagaggc cggttaccgc ctgtttgagg atatagtaat ctttctaaat agctttggat
     1981 tggaggagta tggccactaa tactaagttc agctaataaa aaaatttgct aaagaactcc
     2041 agaaaagtaa gcacctgtta ttgcaataaa attagcctaa ttgagagaag tttctataga
     2101 atttttcata tacttaacga gtgctttcac ctttgaatat agtccttccc acttatcatc
     2161 acactctccc cgatagcctt ttctagctat atccagtaaa gttacatgct ctttaggtaa
     2221 aagaggtata gcccattctg cagcgacatc tttcgaggta atttcaccag tagtcactgt
     2281 ttgccacatt cgagctaggg ttaaaattac attacgctca tcacctttta tcccctcaat
     2341 tagttctggc aaagaatcct taattgctct tcgaatatct gtcaaaggta cggagacaag
     2401 tatacttgaa gaatcaggac caaatagaga aatactattc tttcttgctt gtgctaaaac
     2461 aatagccaaa tcaggatcat agcttggttc ctgaatttgt ccattctcaa attcacccct
     2521 gagccactca ccgtatataa attctctttt tggaggatat tgccaaggga caacttcact
     2581 cctatttata accgtaactt caagtggtct aacagaatcc gtatttccaa tctttcctga
     2641 tatagtcatt agtctttctg ttagtttttt tcgagttaat tgaggtaaac tatgattcac
     2701 gacgactaga acatctacat cgctgttaat gcgtaaacca ccatttactg ctgaaccaaa
     2761 tagatatact ccaactattg aacttccaaa taaatctttt acgattttta atgtttgaat
     2821 cgcttgattt ggtatttttc cgttaatcaa attgctcatg atttcacctc gttgattatg
     2881 ttcatataaa gtttatattg atactcaatt tacttaccct agattggaca tatacttaaa
     2941 ttactgttca ataaagctga ccgttagcgt ttaagtacat cctttcacaa tttgtctaca
     3001 gattaataat tattctttat tatacagatc gatcctctag acctaggcct taagatctga
     3061 tcatatgcat ccgcgggccc gggttaacgc gtaatccatg gatcaagaga caggatgagg
     3121 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggtgc cctgaatgaa
     3181 ctgcagaaag agctggtagt tggcgcactg ttcgaagaac tgccgatgtc cagtaagatt
     3241 cttactatgc tggttgaacc ggatgctggt aaagctactt gggttgctgc ttctacttat
     3301 ggtaccgata caactactgg tgaggaagtt aaaggagctc ttaaagaaat ccacttcagt
     3361 aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg
     3421 cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga
     3481 cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct
     3541 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
     3601 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgacta
     3661 agaaaatgcc gtcaaatccg ctcgccatga cttcactaac gatgcctttg aaaatcttca
     3721 agttcttttc tactaattca aggcgtgtct caccaggttt ttggtttgct ccggcgcaaa
     3781 tgcagacaat atcagcatcc ttgcagggta tgtttctctt tgatgtcttt ttgtttgtga
     3841 agtatttcac atttatattg tgcaacactt cacaaacttt tgcaagagaa aagttttgtc
     3901 tgatttatga acaaaaaaga aaccatcatt gatggtttct ttcggtaagt cccgtctagc
     3961 cttgccctca atggggaaga gaaccgctta agcccgagtc attatataaa ccatttagca
     4021 cgtaatcaaa gccaggctga ttctgaccgg gcacttgggc gctgccatta ttaaaaatca
     4081 cttttgcgtt ggttgtatcc gtgtccgcag gcagcgtcag cgtgtaaatt ccgtctgcat
     4141 ttttagtcat tggttttcca ggccaagatc cggtcaattc aattactcgg ctcccatcat
     4201 gtttatagat ataagcattt acctggctcc aatgattcgg attttgatag ccgatggttt
     4261 tggccgacgc tggatctctt ttaacaaaac tgtatttctc ggtcctcgtt acaccatcac
     4321 tgttcgttcc ttttaacatg atggtgtatg ttttgccaaa ttggatctcc ttttccgatt
     4381 gtgaattgat ctccatcctt aaacgcctgt cgtctggtcc attattgatt tgataaacgg
     4441 cttttgttgt attcgcatct gcacgcaagg taatcgtcag ttgatcattg aaagaatgtg
     4501 ttacacctgt tttgtaattc tcaaggaaaa catgaggcgc ttttgcaata tcatcaggat
     4561 aaagcacagc tacagacctg gcattgatcg tgcctgtcag tttaccatcg ttcacttgaa
     4621 atgaacccgc tccagcttta ttgtcatacc tgccatcagg caattttgtt gccgtattga
     4681 tagagacaga ggatgaacct gcatttgcca gcacaacgcc atgtgagccg cgctgattca
     4741 taaatatctg gttgtttcca ttcgggttcg agagttcctc aggctgtcca gccatcacat
     4801 tgtgaaatct attgaccgca gtgatagcct gatcttcaaa taaagcactc ccgcgatcgc
     4861 ctatttggct tttccccggg aacctcacac catttccgcc tccctcaggt ctggaaaaga
     4921 aaagaggcgt actgcctgaa cgagaagcta tcaccgccca gcctaaacgg atatcatcat
     4981 cgctcatcca tgtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag
     5041 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc
     5101 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctgaatca
     5161 ggctgccgat acggcgaaag cgatgatcca tgaaatgcgt cgtgagatgt cacgctatac
     5221 gtggaaatcc gcgatttatc ttgtactgac gatattcttc gtcctggctt cctgcgtgac
     5281 ggcatttacg tggtttatga atgacgggta tagccagatt gctgaaatgc agaggatgga
     5341 agcggtctgg caaaagaaag ccccgctggc tgacatatcg cgctgtgacg gaaagccctg
     5401 cgttaaggtg gacactcgca ctacgtatgg tgataaagag aatacctgga tgattattaa
     5461 gaaataacat catagagcac agaacgctgt gcgtcgatct ccggcggaga aagcattaat
     5521 taaacgtgtc ggggggatct gataacccca gcaaataaag gacattaagg atatggggtc
     5581 tgaaggccaa tagaacgaaa acgtacgtta gtgaagtaac tgtctgatat atcgaaacat
     5641 aatgtacatt ggaaaacgcc atcaaaacgg tgtcttttta atcgaaaatt ggcacttaac
     5701 ggactttctt gtctactaat cgatacaaat tccccgtagg cgctagggac ctctttagct
     5761 ccttggaagc tgtcagtagt atacctaata atttatctac attcccttta gtaacgtgta
     5821 actttccaaa tttacaaaag cgactcatag aattatttcc tcccgttaaa taatagataa
     5881 ctattaaaaa tagacaatac ttgctcataa gtaacggtac ttaaattgtt tactttggcg
     5941 tgtttcattg cttgatgaaa ctgattttta gtaaacagtt gacgatattc tcgattgacc
     6001 cattttgaaa caaagtacgt atatagcttc caatatttat ctggaacatc tgtggtatgg
     6061 cgggtaagtt ttattaagac actgtttact tttggtttag gatgaaagca ttccgctggc
     6121 agcttaagca attgctgaat cgagacttga gtgtgcaaga gcaaccctag tgttcggtga
     6181 atatccaagg tacgcttgta gaatccttct tcaacaatca gatagatgtc agacgcatgg
     6241 ctttcaaaaa ccactttttt aataatttgt gtgcttaaat ggtaaggaat actcccaaca
     6301 attttatacc tctgtttgtt agggaattga aactgtagaa tatcttggtg aattaaagtg
     6361 acacgagtat tcagttttaa tttttctgac gataagttga atagatgact gtctaattca
     6421 atagacgtta cctgtttact tattttagcc agtttcgtcg ttaaatgccc tttacctgtt
     6481 ccaatttcgt aaacggtatc ggtttctttt aaattcaatt gttttattat ttggttgagt
     6541 actttttcac tcgttaaaaa gttttgagaa tattttatat ttttgttcat gtaatcactc
     6601 cttcttaatt acaaattttt agcatctaat ttaacttcaa ttcctattat acaaaatttt
     6661 aagatactgc actatcaaca cactcttaag tttgcttcta agtcttattt ccataacttc
     6721 ttttacgttt ccgccattct ttgctgtttc gatttttatg atatggtgca agtcagcacg
     6781 aacacgaacc gtcttatctc ccattatatc tttttttgca ctgattggtg tatcatttcg
     6841 tttttctttt tgtgcgccta aatttcccac aatcactcac ttctttctat ttcttcttat
     6901 tcttatttta tcatcaacaa tcacaaatca cttgtgattt gtgataagtg atttgtgatt
     6961 aatatataaa agccctcttt aaagggcttt tatgtttatt ttgagaaaga tataaaatca
     7021 atatatccct tttctccgat ttttacaacg gcattgtagg actttctatc tttcgttttg
     7081 attcctttta ccagggtttc ttttccctct agtaattctt ttacatttgt tttggtgagt
     7141 tttttctttc taaaatgttc agctaaagta aacttacatt caggataatt tgaacaacca
     7201 taaaacgatt tttttaatac aatattgttg ccacacttag gacattttcc tacaatactt
     7261 ttttctgctt ctttttcttt ctgttcctgg taatcagaaa aatttagttt ttctatatcg
     7321 ttaggtacag cttccagtaa atgaacaatg aattttttga tattcgtaat aaagttctct
     7381 tgattgcctt ctcttttacc gatttttttt aaatacgttt cccatttagc cgtcatttca
     7441 gcactcgtta aaaggtgctg actttcaact gcctggcaca ataattttcc tttttcagtt
     7501 acaacaagct ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     7561 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     7621 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     7681 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     7741 gcttgcatgc ctgcaggtcg actctagagg atccccgggt accgagctcg aattcgcggc
     7801 cgcgctagca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
     7861 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgctacatac
     7921 ggaaagctta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
     7981 cttgtcacta ctttgaccta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa
     8041 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct
     8101 ttcaaagatg acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt
     8161 gttaatcgta tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac
     8221 aaactcgagt acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat
     8281 ggaatcaaag ctaacttcaa aattcgccac aacattgaag atggatccgt tcaactagca
     8341 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
     8401 tacctgtcga cacaatctgc cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc
     8461 cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaataa
     8521 taaggccggc caataaagat cctaggacgc cgccaagcca gcttaaaccc agctcaatga
     8581 gctgggtttt ttgtttgtta aaaatgaaga agaaactgtg aagcgtattt aactagtaaa
     8641 atctaagaca tatcatgatt tataaaaaca aaaaaacctt gcaggatatg caaggcttct
     8701 ggatgacccg tacgggattc gaactccgga tgtgagcgcc ggtcgctacc attaccagtt
     8761 ggtctggtgt caaaaataat aataaccggg caggccatgt ctgcccgtat ttcgcgtaag
     8821 gaaatccatt atgtactatc gatcagacca gtttttaatt tgtgtgtttc catgtgtcca
     8881 gtttggaata ctcttaacct cattggaaat cgcggcataa tcactggtgg tatgattgat
     8941 gaccgcgtca acaatgacct ttatgccata ttcttcagcg gctgcacaca tttctttaaa
     9001 ttcttgttca gtacctaagt aacggttgcc aatttgatac gatgtcggct gatacagcca
     9061 gtaccagttc gacatgcttt tatctccttg attcccttcc tttacttggt taatcggaga
     9121 tgtctgaatg gctgtatatc ctgcatcatg aatatccttc atattgtgtt ttaacgtatt
     9181 gaacgaccaa ttccatgcat gaagaatggt tccgcttttg atcgacggtg ctgtaagctc
     9241 attcgatttg ttcgccgttt cagcactcgc agccgccggt cctgccagaa ccaaatgaaa
     9301 cagcaataaa aatccagcga ataacggcag taaagaggtt ttgaatcgtt ttgcaaacat
     9361 tcttgacact ccttatttga ttttttgaag acttacttcg gagtcaaaaa tccctcttac
     9421 ttcattcttc cgcttcctcc tttcaaaccg atgtgaagac tggagaattt tgtt
//
