LOCUS pPEP1 4902 bp DNA CIRCULAR SYN 23-FEB-2015 DEFINITION Pneumococcal Engineering Platform ACCESSION pPEP1 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4902) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software FEATURES Location/Qualifiers misc_signal 10..61 /gene="T_rpsI ->|" /product="Terminator: S. pneumoniae rpsI (forward)" misc_signal complement (66..93) /gene="T_tuf |<-" /product="Terminator: S. pneumoniae tuf (reverse)" misc_feature 106..386 /gene="MCS" /product="Multiple Cloning Site (BglFusion)" misc_signal 137..170 /gene="T_B1002 ->|<-" /product="Terminator: B1002 (synthetic; bidirectional)" misc_signal 195..323 /gene="T_B0015 ->|->|" /product="Terminator: B0015 (E. coli rrnBT1 + T7TE; double forward)" misc_signal 387..426 /gene="T_B1006 ->|<-" /product="Terminator: B1006 (modified) (synthetic; bidirectional)" misc_feature 457..949 /gene="ISD" /product="Integration Site Down (S. pneumoniae D39 1678908-1679400)" misc_signal 1041..1075 /gene="P_CmR ->" /product="Promoter: CmR (-35 to -1)" CDS 1133..1783 /gene="CmR" /product="Chloramphenicol Resistance (Chloramphenicol acetyltransferase)" misc_signal 1796..1824 /gene="T_CmR ->|" /product="Terminator: CmR" misc_feature 1844..2481 /gene="Ori" /product="Ori+ origin of replication (without repA nonfunctional; low copy)" misc_feature 2488..3334 /gene="15A" /product="p15A origin of replication (low to medium copy; 10-12 plasmids/cell)" misc_feature 3344..3819 /gene="ISU" /product="Integration Site Up (S. pneumoniae D39 1678030-1678505)" misc_signal complement (3840..3865) /gene="T_SpR |<-" /product="Terminator: SpR" CDS complement (3900..4682) /gene="SpR" /product="Spectinomycin Resistance (Streptomycin 3''-adenylyltransferase)" misc_signal complement (4829..4866) /gene="P_SpR <-" /product="Promoter: SpR (-35 to -1) (synthetic)" BASE COUNT 1436 a 1001 c 965 g 1500 t ORIGIN 1 gcatgcggca aagcactcaa aagtttacct tatgggtgct tttttcgtgc ttttttgaaa 61 agacaaaaaa aagaaccttg ccaagcaaga ttcttttgat agggtggtct ccgatcgtgt 121 actgcagaaa actagtcgca aaaaaccccg cttcggcggg gttttttcgc tctagacggt 181 gatcaacacg ctagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 241 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 301 gggtgggcct ttctgcgttt atagaattca tgagatctaa ataaataagg aggaaaatta 361 atgccggatc cctccagtaa ctcgagaaaa aaaaaccgcg cccctgacag ggcgcggttt 421 ttttttaagt ggacacctac gtatggtaac tgtacaggtc gctgaaactt ctatcgtcaa 481 gaagaatcac caaatccctc gtatcatcaa ccaaaagatt gctcaaaagt taattgaaaa 541 gatttctatg actgatattg cccatcagct ttccatctca acttcaactg ttattcgtaa 601 gctcaatgat tttcacttta aacatgattt ttcgcgtctt cctgagatta tgtcttggga 661 tgagtatgct tttacaaagg gaaagatgag cttcattgcg caagatttta acaatctcaa 721 tatcatcact gttcttgagg gaagaacaca agctatcatt cgaaatcact ttcttcgcta 781 cgatagagtt gtccgatgtc gcgtcaaaat tattactatg gatatgttta gcccttacta 841 tgacttggct aaacagcttc gctttcaaat ttctaggctc aggctgaaac agtctcccag 901 actgtttcac tcccgaatgc taaaatcgtt cttgatcgct ttcacattgg acgtcctgac 961 catgggcacc cattagttca acaaacgaaa attggataaa gtgggatatt tttaaaatat 1021 atatttatgt tacagtaata ttgactttta aaaaaggatt gattctaatg aagaaagcag 1081 acaagtaagc ctcctaaatt cactttagat aaaaatttag gaggcatatc aaatgaactt 1141 taataaaatt gatttagata attggaagag aaaagagata tttaatcatt atttgaacca 1201 acaaacgact tttagtataa ccacagaaat tgatattagt gttttatacc gaaacataaa 1261 acaagaagga tataaatttt accctgcatt tattttctta gtgacaaggg tgataaactc 1321 aaatacagct tttagaactg gttacaatag cgacggagag ttaggttatt gggataagtt 1381 agagccactt tatacaattt ttgatggtgt atctaaaaca ttctctggta tttggactcc 1441 tgtaaagaat gacttcaaag agttttatga tttatacctt tctgatgttg agaaatataa 1501 tggttcgggg aaattgtttc ccaaaacacc tataccagaa aatgcttttt ctctttctat 1561 tattccttgg acttcattta ctgggtttaa cttaaatatc aataataata gtaattacct 1621 tctacccatt attacagcag gaaaattcat caataaaggt aattcaatat atttaccgct 1681 atctttacag gtacatcatt ctgtttgtga tggttatcat gcaggattgt ttatgaactc 1741 tattcaggaa ttgtcagata ggcctaatga ctggctttta taatatgaga taatgccgac 1801 tgtacttttt acagtcggtt ttctaatgtc actaaccggt accttgtgaa tcgggtcgag 1861 gcctcggact agcgattttt tattaaaacg tctcaaaatc gtttctgaga cgttttagcg 1921 tttatttcgt ttagttatcg gcataatcgt taaaacaggc gttatcgtag cgtaaaagcc 1981 cttgagcgta gcgtggcttt gcagcgaaga tgttgtctgt tagattatga aagccgatga 2041 ctgaatgaaa taataagcgc agcgcccttc tatttcggtt ggaggaggct caagggagta 2101 tgagggaatg aaattccctc atgggtttga ttttaaaaat tgcttgcaat tttgccgagc 2161 ggtagcgctg gaaaattttt gaaaaaaatt tggaatttgg aaaaaaatgg ggggaaagga 2221 agcgaatttt gcttccgtac tacgaccccc cattaagtgc cgagtgccaa tttttgtgcc 2281 aaaaacgctc tatcccaact ggctcaaggg tttaaggggt ttttcaatcg ccaacgaatc 2341 gccaacgttt tcgccaacgt tttttataaa tctatattta agtagcttta ttgttgtttt 2401 tatgattaca aagtgataca ctaactttat aaaattattt gattggagtt ttttaaatgg 2461 tgatttcaga atcgctagtt cgagctcgtt ccactgagcg tcagacccct taataagatg 2521 atcttcttga gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc 2581 cttgcagggc ggttttttga aggttctctg agctaccaac tctttgaacc gaggtaactg 2641 gcttggagga gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcgcatga 2701 cttcaagact aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat 2761 gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa 2821 cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca 2881 ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca 2941 ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct 3001 gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc agggggcgga 3061 gcctatggaa aaacggcttt gccgcggccc tctcacttcc ctgttaagta tcttcctggc 3121 atcttccagg aaatctccgc cccgttcgta agccatttcc gctcgccgca gtcgaacgac 3181 cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc ctgtatcaca tattctgctg 3241 acgcaccggt gcagcctttt ttctcctgcc acatgaagca cttcactgac accctcatca 3301 gtgccaacat agtaagccag tatacactcc gctaggcggc cgcagttaaa gaacttggac 3361 tgcgctctaa aaccaacgtg gtcagtctgg acaagattat tattgataaa aaatcctcac 3421 tgataaccgg tttcccagag tttcggatgg tttggaaggt ggtccgccag cgtgtggtgg 3481 atgatctggt atccgttctg gatacggact atctggatat ggaactaatc ccaaatctca 3541 ctcgccaaat cgctgagcag tctatctatt cttatataga aaatggcctc aaactcctta 3601 ttgattatgc tcagaaggaa atcaccattg accactcaag cgaccgagac aagattctca 3661 tggacattgg caaagaccct tatgtcgttt cgattaaatc aaaagtctat ctccaagacg 3721 gacgccaatt tcagtttacc gaaagtcgcc ataagttaga aaaatttaga tttgtagatt 3781 ttgcaaaacg caagaaataa aagactgaga caccagatca tggctagaaa gcttctagca 3841 aaaaactgga cggagtcccg tccagaaaag taagcacttg ttattgcaat aaaattagcc 3901 taattgagag aagtttctat agaatttttc atatacttaa cgagtgcttt cacctttgaa 3961 tatagtcctt cccacttatc atcacactct ccccgatagc cttttctagc tatatccagt 4021 aaagttacat gctctttagg taaaagaggt atagcccatt cagcagcgac atctttcgag 4081 gtaatttcac cagtagtcac tgtttgccac attcgagcta gggttaaaat tacattacgc 4141 tcatcacctt ttatcccctc aattagttct ggcaaagaat ccttaattgc tcttcgaata 4201 tctgtcaaag gtacggagac aagtatactt gaagaatcag gaccaaatag agaaatacta 4261 ttctttcttg cttgtgctaa aacaatagcc aaatcaggat catagcttgg ttcctgaatt 4321 tgtccattct caaattcacc cctgagccac tcaccgtata taaattctct ttttggagga 4381 tattgccaag ggacaacttc actcctattt ataaccgtaa cttcaagtgg tctaacagaa 4441 tccgtatttc caatccttcc tgatatagtc attagtcttt ctgttagttt ttttcgagtt 4501 aattgaggta aactatgatt cacgacgact agaacatcta catcgctgtt aatgcgtaaa 4561 ccaccattta ctgctgaacc aaatagatat actccaacta ttgaacttcc aaataaatct 4621 tttacgattt ttaatgtttg aatcgcttga tttggtattt ttccgttaat caaattgctc 4681 atgatttcac ctcgttgatt atgttcatat aaagtttata ttgatactca atttacttac 4741 cctagattgg acatatactt aaattactgt tcaataaagc tgaccgttag cgtttaagta 4801 catcctttca caatttgtct acagaacagt actggctatt atatcagcct tcttattcaa 4861 tgtcaagaat aaactgttct atgtaaacct attcattgtt tt //