LOCUS       pPEP1        4902 bp    DNA   CIRCULAR SYN        23-FEB-2015
DEFINITION  Pneumococcal Engineering Platform
ACCESSION   pPEP1
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_signal     10..61
                     /gene="T_rpsI ->|"
                     /product="Terminator: S. pneumoniae rpsI (forward)"
     misc_signal     complement (66..93)
                     /gene="T_tuf |<-"
                     /product="Terminator: S. pneumoniae tuf (reverse)"
     misc_feature    106..386
                     /gene="MCS"
                     /product="Multiple Cloning Site (BglFusion)"
     misc_signal     137..170
                     /gene="T_B1002 ->|<-"
                     /product="Terminator: B1002 (synthetic; bidirectional)"
     misc_signal     195..323
                     /gene="T_B0015 ->|->|"
                     /product="Terminator: B0015 (E. coli rrnBT1 + T7TE; double forward)"
     misc_signal     387..426
                     /gene="T_B1006 ->|<-"
                     /product="Terminator: B1006 (modified) (synthetic; bidirectional)"
     misc_feature    457..949
                     /gene="ISD"
                     /product="Integration Site Down (S. pneumoniae D39 1678908-1679400)"
     misc_signal     1041..1075
                     /gene="P_CmR ->"
                     /product="Promoter: CmR (-35 to -1)"
     CDS             1133..1783
                     /gene="CmR"
                     /product="Chloramphenicol Resistance (Chloramphenicol acetyltransferase)"
     misc_signal     1796..1824
                     /gene="T_CmR ->|"
                     /product="Terminator: CmR"
     misc_feature    1844..2481
                     /gene="Ori"
                     /product="Ori+ origin of replication (without repA nonfunctional; low copy)"
     misc_feature    2488..3334
                     /gene="15A"
                     /product="p15A origin of replication (low to medium copy; 10-12 plasmids/cell)"
     misc_feature    3344..3819
                     /gene="ISU"
                     /product="Integration Site Up (S. pneumoniae D39 1678030-1678505)"
     misc_signal     complement (3840..3865)
                     /gene="T_SpR |<-"
                     /product="Terminator: SpR"
     CDS             complement (3900..4682)
                     /gene="SpR"
                     /product="Spectinomycin Resistance (Streptomycin 3''-adenylyltransferase)"
     misc_signal     complement (4829..4866)
                     /gene="P_SpR <-"
                     /product="Promoter: SpR (-35 to -1) (synthetic)"
BASE COUNT     1436 a   1001 c    965 g   1500 t
ORIGIN      
        1 gcatgcggca aagcactcaa aagtttacct tatgggtgct tttttcgtgc ttttttgaaa
       61 agacaaaaaa aagaaccttg ccaagcaaga ttcttttgat agggtggtct ccgatcgtgt
      121 actgcagaaa actagtcgca aaaaaccccg cttcggcggg gttttttcgc tctagacggt
      181 gatcaacacg ctagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      241 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      301 gggtgggcct ttctgcgttt atagaattca tgagatctaa ataaataagg aggaaaatta
      361 atgccggatc cctccagtaa ctcgagaaaa aaaaaccgcg cccctgacag ggcgcggttt
      421 ttttttaagt ggacacctac gtatggtaac tgtacaggtc gctgaaactt ctatcgtcaa
      481 gaagaatcac caaatccctc gtatcatcaa ccaaaagatt gctcaaaagt taattgaaaa
      541 gatttctatg actgatattg cccatcagct ttccatctca acttcaactg ttattcgtaa
      601 gctcaatgat tttcacttta aacatgattt ttcgcgtctt cctgagatta tgtcttggga
      661 tgagtatgct tttacaaagg gaaagatgag cttcattgcg caagatttta acaatctcaa
      721 tatcatcact gttcttgagg gaagaacaca agctatcatt cgaaatcact ttcttcgcta
      781 cgatagagtt gtccgatgtc gcgtcaaaat tattactatg gatatgttta gcccttacta
      841 tgacttggct aaacagcttc gctttcaaat ttctaggctc aggctgaaac agtctcccag
      901 actgtttcac tcccgaatgc taaaatcgtt cttgatcgct ttcacattgg acgtcctgac
      961 catgggcacc cattagttca acaaacgaaa attggataaa gtgggatatt tttaaaatat
     1021 atatttatgt tacagtaata ttgactttta aaaaaggatt gattctaatg aagaaagcag
     1081 acaagtaagc ctcctaaatt cactttagat aaaaatttag gaggcatatc aaatgaactt
     1141 taataaaatt gatttagata attggaagag aaaagagata tttaatcatt atttgaacca
     1201 acaaacgact tttagtataa ccacagaaat tgatattagt gttttatacc gaaacataaa
     1261 acaagaagga tataaatttt accctgcatt tattttctta gtgacaaggg tgataaactc
     1321 aaatacagct tttagaactg gttacaatag cgacggagag ttaggttatt gggataagtt
     1381 agagccactt tatacaattt ttgatggtgt atctaaaaca ttctctggta tttggactcc
     1441 tgtaaagaat gacttcaaag agttttatga tttatacctt tctgatgttg agaaatataa
     1501 tggttcgggg aaattgtttc ccaaaacacc tataccagaa aatgcttttt ctctttctat
     1561 tattccttgg acttcattta ctgggtttaa cttaaatatc aataataata gtaattacct
     1621 tctacccatt attacagcag gaaaattcat caataaaggt aattcaatat atttaccgct
     1681 atctttacag gtacatcatt ctgtttgtga tggttatcat gcaggattgt ttatgaactc
     1741 tattcaggaa ttgtcagata ggcctaatga ctggctttta taatatgaga taatgccgac
     1801 tgtacttttt acagtcggtt ttctaatgtc actaaccggt accttgtgaa tcgggtcgag
     1861 gcctcggact agcgattttt tattaaaacg tctcaaaatc gtttctgaga cgttttagcg
     1921 tttatttcgt ttagttatcg gcataatcgt taaaacaggc gttatcgtag cgtaaaagcc
     1981 cttgagcgta gcgtggcttt gcagcgaaga tgttgtctgt tagattatga aagccgatga
     2041 ctgaatgaaa taataagcgc agcgcccttc tatttcggtt ggaggaggct caagggagta
     2101 tgagggaatg aaattccctc atgggtttga ttttaaaaat tgcttgcaat tttgccgagc
     2161 ggtagcgctg gaaaattttt gaaaaaaatt tggaatttgg aaaaaaatgg ggggaaagga
     2221 agcgaatttt gcttccgtac tacgaccccc cattaagtgc cgagtgccaa tttttgtgcc
     2281 aaaaacgctc tatcccaact ggctcaaggg tttaaggggt ttttcaatcg ccaacgaatc
     2341 gccaacgttt tcgccaacgt tttttataaa tctatattta agtagcttta ttgttgtttt
     2401 tatgattaca aagtgataca ctaactttat aaaattattt gattggagtt ttttaaatgg
     2461 tgatttcaga atcgctagtt cgagctcgtt ccactgagcg tcagacccct taataagatg
     2521 atcttcttga gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc
     2581 cttgcagggc ggttttttga aggttctctg agctaccaac tctttgaacc gaggtaactg
     2641 gcttggagga gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcgcatga
     2701 cttcaagact aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat
     2761 gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa
     2821 cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca
     2881 ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca
     2941 ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct
     3001 gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc agggggcgga
     3061 gcctatggaa aaacggcttt gccgcggccc tctcacttcc ctgttaagta tcttcctggc
     3121 atcttccagg aaatctccgc cccgttcgta agccatttcc gctcgccgca gtcgaacgac
     3181 cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc ctgtatcaca tattctgctg
     3241 acgcaccggt gcagcctttt ttctcctgcc acatgaagca cttcactgac accctcatca
     3301 gtgccaacat agtaagccag tatacactcc gctaggcggc cgcagttaaa gaacttggac
     3361 tgcgctctaa aaccaacgtg gtcagtctgg acaagattat tattgataaa aaatcctcac
     3421 tgataaccgg tttcccagag tttcggatgg tttggaaggt ggtccgccag cgtgtggtgg
     3481 atgatctggt atccgttctg gatacggact atctggatat ggaactaatc ccaaatctca
     3541 ctcgccaaat cgctgagcag tctatctatt cttatataga aaatggcctc aaactcctta
     3601 ttgattatgc tcagaaggaa atcaccattg accactcaag cgaccgagac aagattctca
     3661 tggacattgg caaagaccct tatgtcgttt cgattaaatc aaaagtctat ctccaagacg
     3721 gacgccaatt tcagtttacc gaaagtcgcc ataagttaga aaaatttaga tttgtagatt
     3781 ttgcaaaacg caagaaataa aagactgaga caccagatca tggctagaaa gcttctagca
     3841 aaaaactgga cggagtcccg tccagaaaag taagcacttg ttattgcaat aaaattagcc
     3901 taattgagag aagtttctat agaatttttc atatacttaa cgagtgcttt cacctttgaa
     3961 tatagtcctt cccacttatc atcacactct ccccgatagc cttttctagc tatatccagt
     4021 aaagttacat gctctttagg taaaagaggt atagcccatt cagcagcgac atctttcgag
     4081 gtaatttcac cagtagtcac tgtttgccac attcgagcta gggttaaaat tacattacgc
     4141 tcatcacctt ttatcccctc aattagttct ggcaaagaat ccttaattgc tcttcgaata
     4201 tctgtcaaag gtacggagac aagtatactt gaagaatcag gaccaaatag agaaatacta
     4261 ttctttcttg cttgtgctaa aacaatagcc aaatcaggat catagcttgg ttcctgaatt
     4321 tgtccattct caaattcacc cctgagccac tcaccgtata taaattctct ttttggagga
     4381 tattgccaag ggacaacttc actcctattt ataaccgtaa cttcaagtgg tctaacagaa
     4441 tccgtatttc caatccttcc tgatatagtc attagtcttt ctgttagttt ttttcgagtt
     4501 aattgaggta aactatgatt cacgacgact agaacatcta catcgctgtt aatgcgtaaa
     4561 ccaccattta ctgctgaacc aaatagatat actccaacta ttgaacttcc aaataaatct
     4621 tttacgattt ttaatgtttg aatcgcttga tttggtattt ttccgttaat caaattgctc
     4681 atgatttcac ctcgttgatt atgttcatat aaagtttata ttgatactca atttacttac
     4741 cctagattgg acatatactt aaattactgt tcaataaagc tgaccgttag cgtttaagta
     4801 catcctttca caatttgtct acagaacagt actggctatt atatcagcct tcttattcaa
     4861 tgtcaagaat aaactgttct atgtaaacct attcattgtt tt
//
