LOCUS pCRII-TOPO-eGFPb 5759 bp DNA circular UNA 23-OCT-2014 DEFINITION eGFP targeting vector. ACCESSION urn:local:.:1379934465003.2280 (modified) VERSION KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers source 1..>591 /organism="pCRII-TOPO" /mol_type="other DNA" /label="source pCRII-TOPO" promoter 143..172 /label="lac_promoter" misc_feature 186..208 /label="M13_pUC_rev_primer" misc_feature 207..225 /label="M13_reverse_primer" misc_feature 239..256 /label="Sp6_primer" misc_feature 337..572 /note="Geneious type: Editing History Insertion" /label="misc" misc_feature 337..572 /note="Geneious type: motif" /label="eGFP bait sequence" misc_feature 447..469 /note="Geneious type: motif" /label="sgRNA binding site" misc_feature 606..665 /note="Geneious type: motif" /label="E2A" misc_feature <666..2162 /note="Geneious type: Inserted sequence" /label="KaltA4-gI-pA" source <2163..5759 /organism="pCRII-TOPO" /mol_type="other DNA" /label="source pCRII-TOPO" promoter complement(2193..2211) /label="T7_promoter" misc_feature 2215..2370 /label="lacZ_a" misc_feature complement(2218..2234) /label="M13_forward20_primer" misc_feature complement(2227..2249) /label="M13_pUC_fwd_primer" rep_origin 2392..2698 /label="f1_origin" promoter 3009..3058 /label="NEOKAN_promoter" CDS 3147..3941 /gene="NeoR/KanR" /note="ORF frame 2" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" gene 3150..3938 /gene="NeoR/KanR" /label="NeoR/KanR" CDS 3959..4819 /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" /label="Ampicillin" gene 3959..4819 /gene="Ampicillin" /label="Ampicillin" rep_origin 4974..5593 /label="pBR322_origin" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat 241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca 301 ctagtaacgg ccgccagtgt gctggaattc gcccttatag tggtaccatg gtgagcaagg 361 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 421 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 481 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 541 tgacctacgg cgtgcagtgc ttcagccgct acaagggcga attctgcaga tatccaggag 601 gaggacagtg tactaattat gctctcttga aattggctgg agatgttgag agcaacccag 661 gtcccggagg aggaaaactg ctctcatcca tcgagcaagc ctgcgacatt tgtcggctta 721 agaagctgaa atgctccaag gaaaagccga aatgtgccaa atgcctgaag aacaattggg 781 aatgtcgtta ctctcccaaa accaagcgaa gtccactcac aagggctcat ctgaccgaag 841 tggagagcag gctagagaga ctggaacaac tctttttgct catcttccct agagaggacc 901 ttgacatgat cctcaagatg gattctctcc aggatattaa agcccttttg actggcttat 961 tcgtccagga caatgtgaac aaagacgctg tgacagaccg attggcaagt gtcgagaccg 1021 atatgcctct gacactgaga cagcacagaa tcagcgctac ttcctcaagc gaagagtctt 1081 ctaacaaggg acagagacag ctgactgttt cgagcaggtc gaccccgtcc ccggccgacg 1141 ccctggacga cggcgacctg gacatgctgc ctgctgatgc tctcgatgat ttcgatctgg 1201 atatgctccc ggccgacgcc ctggacgact acgacctgga catcctcccg ggtaactaag 1261 taaggatctc gaccgatcct gagaacttca gggtgagttt ggggaccctt gattgttctt 1321 tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt 1381 ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc 1441 actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt 1501 tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt 1561 cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt 1621 gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt agaatatttc 1681 tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacaccctgg 1741 tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa 1801 aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctctttcc 1861 tacagctcct gggcaacgtg ctggttgttg tgctgtctca tcattttggc aaagaattaa 1921 ttcctcgacg gatcgtagat ccagacatga taagatacat tgatgagttt ggacaaacca 1981 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat 2041 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt 2101 ttcaggttca gggggaggtg tgggaggttt tttaattcgc ggccgctcta gaactagtgg 2161 actcgagcat gcatctagag ggcccaattc gccctatagt gagtcgtatt acaattcact 2221 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct 2281 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc 2341 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc 2401 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 2461 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 2521 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 2581 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 2641 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 2701 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 2761 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aattcagggc 2821 gcaagggctg ctaaaggaag cggaacacgt agaaagccag tccgcagaaa cggtgctgac 2881 cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa 2941 agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag 3001 caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag 3061 taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca agatctgatc 3121 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 3181 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 3241 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 3301 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca 3361 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 3421 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc ccaccttgct cctgccgaga 3481 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 3541 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 3601 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 3661 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 3721 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 3781 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 3841 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 3901 agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattgaaaaa ggaagagtat 3961 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 4021 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 4081 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 4141 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 4201 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 4261 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 4321 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 4381 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 4441 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 4501 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 4561 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 4621 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 4681 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 4741 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 4801 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 4861 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 4921 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 4981 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 5041 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 5101 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 5161 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 5221 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 5281 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 5341 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 5401 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 5461 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 5521 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 5581 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 5641 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 5701 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaag //