LOCUS       pMM178                  9390 bp    DNA     circular UNA 16-OCT-2014
DEFINITION  pRGNndm-1.
ACCESSION   urn.local...1408041832304.71
VERSION     urn.local...1408041832304.71
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     terminator      6..110
                     /created_by="User"
                     /Transferred_From="pZA11-MCS PCR Product 2"
                     /Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /label="Term T1"
     ncRNA           126..463
                     /created_by="User"
                     /modified_by="User"
                     /label="crRNA"
     promoter        166..171
                     /created_by="User"
                     /label
     promoter        184..194
                     /created_by="User"
                     /label
     repeat_unit     296..331
                     /created_by="User"
                     /label="R"
     misc_feature    332..361
                     /created_by="User"
                     /modified_by="User"
                     /label="ndm1"
                     /note="Geneious type: sequence variant"
     repeat_unit     362..397
                     /created_by="User"
                     /label="R"
     terminator      486..515
                     /created_by="User"
                     /modified_by="User"
                     /label="tL17"
     rep_origin      516..3848
                     /created_by="User"
                     /modified_by="User"
                     /label="pBBR1 ori"
     terminator      3855..3960
                     /created_by="User"
                     /label="Term t0"
     CDS             complement(3966..4762)
                     /modified_by="User"
                     /label="cat"
     ncRNA           complement(4846..5144)
                     /created_by="User"
                     /Transferred_From="SPtracrRNA-pLtetO"
                     /Transferred_Similarity="100.00%"
                     /label="tracrRNA"
     terminator      4901..4937
                     /created_by="User"
                     /Transferred_From="SPtracrRNA-pLtetO"
                     /Transferred_Similarity="100.00%"
                     /label="Term"
     promoter        5151..5224
                     /created_by="User"
                     /Transferred_From="SPtracrRNA-pLtetO"
                     /Transferred_Similarity="100.00%"
                     /label="pLtetO"
     gene            5278..9384
                     /created_by="User"
                     /Transferred_From="MBP-Cas9 Strep pyogenes PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="cas9"
ORIGIN      
        1 ctagaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
       61 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cctagcaccc
      121 tgcagaacta tatgattttc cgcagtatat tttagatgaa gattatttct taataactaa
      181 aaatatggta taatactctt aataaatgca gtaatacagg ggcttttcaa gactgaagtc
      241 tagctgagac aaatagtgcg attacgaaat tttttagaca aaaatagtct acgaggtttt
      301 agagctatgc tgttttgaat ggtcccaaaa cgggcagtcg cttccaacgg tttgatcgtc
      361 agttttagag ctatgctgtt ttgaatggtc ccaaaacttc agcacactga gacttgttga
      421 gttccatgtt ttagagctat gctgttttga atggtctcca ttctctagat gatgattaag
      481 gatctatttt tttgggcggg gccgcccaaa aaaatggttc gcttgctgtc cataaaaccg
      541 cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg
      601 cgttttccct tgtccagata gcccagtagc tgacattcat cccaggtggc acttttcggg
      661 gaaatgtgcg cgcccgcgtt cctgctggcg ctgggcctgt ttctggcgct ggacttcccg
      721 ctgttccgtc agcagctttt cgcccacggc cttgatgatc gcggcggcct tggcctgcat
      781 atcccgattc aacggcccca gggcgtccag aacgggcttc aggcgctccc gaaggtctcg
      841 ggccgtctct tgggcttgat cggccttctt gcgcatctca cgcgctcctg cggcggcctg
      901 tagggcaggc tcatacccct gccgaaccgc ttttgtcagc cggtcggcca cggcttccgg
      961 cgtctcaacg cgctttgaga ttcccagctt ttcggccaat ccctgcggtg cataggcgcg
     1021 tggctcgacc gcttgcgggc tgatggtgac gtggcccact ggtggccgct ccagggcctc
     1081 gtagaacgcc tgaatgcgcg tgtgacgtgc cttgctgccc tcgatgcccc gttgcagccc
     1141 tagatcggcc acagcggccg caaacgtggt ctggtcgcgg gtcatctgcg ctttgttgcc
     1201 gatgaactcc ttggccgaca gcctgccgtc ctgcgtcagc ggcaccacga acgcggtcat
     1261 gtgcgggctg gtttcgtcac ggtggatgct ggccgtcacg atgcgatccg ccccgtactt
     1321 gtccgccagc cacttgtgcg ccttctcgaa gaacgccgcc tgctgttctt ggctggccga
     1381 cttccaccat tccgggctgg ccgtcatgac gtactcgacc gccaacacag cgtccttgcg
     1441 ccgcttctct ggcagcaact cgcgcagtcg gcccatcgct tcatcggtgc tgctggccgc
     1501 ccagtgctcg ttctctggcg tcctgctggc gtcagcgttg ggcgtctcgc gctcgcggta
     1561 ggcgtgcttg agactggccg ccacgttgcc cattttcgcc agcttcttgc atcgcatgat
     1621 cgcgtatgcc gccatgcctg cccctccctt ttggtgtcca accggctcga cgggggcagc
     1681 gcaaggcggt gcctccggcg ggccactcaa tgcttgagta tactcactag actttgcttc
     1741 gcaaagtcgt gaccgcctac ggcggctgcg gcgccctacg ggcttgctct ccgggcttcg
     1801 ccctgcgcgg tcgctgcgct cccttgccag cccgtggata tgtggacgat ggccgcgagc
     1861 ggccaccggc tggctcgctt cgctcggccc gtggacaacc ctgctggaca agctgatgga
     1921 caggctgcgc ctgcccacga gcttgaccac agggattgcc caccggctac ccagccttcg
     1981 accacatacc caccggctcc aactgcgcgg cctgcggcct tgccccatca atttttttaa
     2041 ttttctctgg ggaaaagcct ccggcctgcg gcctgcgcgc ttcgcttgcc ggttggacac
     2101 caagtggaag gcgggtcaag gctcgcgcag cgaccgcgca gcggcttggc cttgacgcgc
     2161 ctggaacgac ccaagcctat gcgagtgggg gcagtcgaag gcgaagcccg cccgcctgcc
     2221 ccccgagcct cacggcggcg agtgcggggg ttccaagggg gcagcgccac cttgggcaag
     2281 gccgaaggcc gcgcagtcga tcaacaagcc ccggaggggc cactttttgc cggaggggga
     2341 gccgcgccga aggcgtgggg gaaccccgca ggggtgccct tctttgggca ccaaagaact
     2401 agatataggg cgaaatgcga aagacttaaa aatcaacaac ttaaaaaagg ggggtacgca
     2461 acagctcatt gcggcacccc ccgcaatagc tcattgcgta ggttaaagaa aatctgtaat
     2521 tgactgccac ttttacgcaa cgcataattg ttgtcgcgct gccgaaaagt tgcagctgat
     2581 tgcgcatggt gccgcaaccg tgcggcaccc taccgcatgg agataagcat ggccacgcag
     2641 tccagagaaa tcggcattca agccaagaac aagcccggtc actgggtgca aacggaacgc
     2701 aaagcgcatg aggcgtgggc cgggcttatt gcgaggaaac ccacggcggc aatgctgctg
     2761 catcacctcg tggcgcagat gggccaccag aacgccgtgg tggtcagcca gaagacactt
     2821 tccaagctca tcggacgttc tttgcggacg gtccaatacg cagtcaagga cttggtggcc
     2881 gagcgctgga tctccgtcgt gaagctcaac ggccccggca ccgtgtcggc ctacgtggtc
     2941 aatgaccgcg tggcgtgggg ccagccccgc gaccagttgc gcctgtcggt gttcagtgcc
     3001 gccgtggtgg ttgatcacga cgaccaggac gaatcgctgt tggggcatgg cgacctgcgc
     3061 cgcatcccga ccctgtatcc gggcgagcag caactaccga ccggccccgg cgaggagccg
     3121 cccagccagc ccggcattcc gggcatggaa ccagacctgc cagccttgac cgaaacggag
     3181 gaatgggaac ggcgcgggca gcagcgcctg ccgatgcccg atgagccgtg ttttctggac
     3241 gatggcgagc cgttggagcc gccgacacgg gtcacgctgc cgcgccggta gcacttgggt
     3301 tgcgcagcaa cccgtaagtg cgctgttcca gactatcggc tgtagccgcc tcgccgccct
     3361 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
     3421 aatggaagcc ggcggcacct cgctaacgga ttcaccgttt ttatcaggct ctgggaggca
     3481 gaataaatga tcatatcgtc aattattacc tccacgggga gagcctgagc aaactggcct
     3541 caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag
     3601 caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg
     3661 ctttcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag caccaggcgt
     3721 ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagtc
     3781 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     3841 attaacgcac tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     3901 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     3961 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     4021 aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg
     4081 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     4141 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     4201 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     4261 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     4321 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     4381 ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag
     4441 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     4501 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     4561 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     4621 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     4681 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac
     4741 gtctcatttt cgccagatat cgacgtctaa gaaaccatta ttatcatgac attaacctat
     4801 aaaaataggc gtatcacgag gccctttcgt cttcaccctg tcgacgcgtt ttgaaagcgc
     4861 ttgttttttt ggtttgcagt cagagtagaa tagaagtatc aaaaaaagca ccgactcggt
     4921 gccacttttt caagttgata acggactagc cttattttaa cttgctatgc tgttttgaat
     4981 ggttccaaca agattatttt ataactttta taacaaataa tcaaggagaa attcaaagaa
     5041 atttatcagc cataaaacaa tacttaatac tatagaatga taacaaaata aactactttt
     5101 taaaagaatt ttgtgttata atctatttat tattaagtat tgggatcgat tccctatcag
     5161 tgatagagat tgacatccct atcagtgata gagatactga gcacatcagc aggacgcact
     5221 gaccgaattc attaaagagg agaaaggtgc ggccgcatgc atcaccatca ccatcacatg
     5281 gataagaaat actcaatagg cttagatatc ggcacaaata gcgtcggatg ggcggtgatc
     5341 actgatgaat ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac agaccgccac
     5401 agtatcaaaa aaaatcttat aggggctctt ttatttgaca gtggagagac agcggaagcg
     5461 actcgtctca aacggacagc tcgtagaagg tatacacgtc ggaagaatcg tatttgttat
     5521 ctacaggaga ttttttcaaa tgagatggcg aaagtagatg atagtttctt tcatcgactt
     5581 gaagagtctt ttttggtgga agaagacaag aagcatgaac gtcatcctat ttttggaaat
     5641 atagtagatg aagttgctta tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa
     5701 ttggtagatt ctactgataa agcggatttg cgcttaatct atttggcctt agcgcatatg
     5761 attaagtttc gtggtcattt tttgattgag ggagatttaa atcctgataa tagtgatgtg
     5821 gacaaactat ttatccagtt ggtacaaacc tacaatcaat tatttgaaga aaaccctatt
     5881 aacgcaagtg gagtagatgc taaagcgatt ctttctgcac gattgagtaa atcaagacga
     5941 ttagaaaatc tcattgctca gctccccggt gagaagaaaa atggcttatt tgggaatctc
     6001 attgctttgt cattgggttt gacccctaat tttaaatcaa attttgattt ggcagaagat
     6061 gctaaattac agctttcaaa agatacttac gatgatgatt tagataattt attggcgcaa
     6121 attggagatc aatatgctga tttgtttttg gcagctaaga atttatcaga tgctatttta
     6181 ctttcagata tcctaagagt aaatactgaa ataactaagg ctcccctatc agcttcaatg
     6241 attaaacgct acgatgaaca tcatcaagac ttgactcttt taaaagcttt agttcgacaa
     6301 caacttccag aaaagtataa agaaatcttt tttgatcaat caaaaaacgg atatgcaggt
     6361 tatattgatg ggggagctag ccaagaagaa ttttataaat ttatcaaacc aattttagaa
     6421 aaaatggatg gtactgagga attattggtg aaactaaatc gtgaagattt gctgcgcaag
     6481 caacggacct ttgacaacgg ctctattccc catcaaattc acttgggtga gctgcatgct
     6541 attttgagaa gacaagaaga cttttatcca tttttaaaag acaatcgtga gaagattgaa
     6601 aaaatcttga cttttcgaat tccttattat gttggtccat tggcgcgtgg caatagtcgt
     6661 tttgcatgga tgactcggaa gtctgaagaa acaattaccc catggaattt tgaagaagtt
     6721 gtcgataaag gtgcttcagc tcaatcattt attgaacgca tgacaaactt tgataaaaat
     6781 cttccaaatg aaaaagtact accaaaacat agtttgcttt atgagtattt tacggtttat
     6841 aacgaattga caaaggtcaa atatgttact gaaggaatgc gaaaaccagc atttctttca
     6901 ggtgaacaga agaaagccat tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt
     6961 aagcaattaa aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca
     7021 ggagttgaag atagatttaa tgcttcatta ggtacctacc atgatttgct aaaaattatt
     7081 aaagataaag attttttgga taatgaagaa aatgaagata tcttagagga tattgtttta
     7141 acattgacct tatttgaaga tagggagatg attgaggaaa gacttaaaac atatgctcac
     7201 ctctttgatg ataaggtgat gaaacagctt aaacgtcgcc gttatactgg ttggggacgt
     7261 ttgtctcgaa aattgattaa tggtattagg gataagcaat ctggcaaaac aatattagat
     7321 tttttgaaat cagatggttt tgccaatcgc aattttatgc agctgatcca tgatgatagt
     7381 ttgacattta aagaagacat tcaaaaagca caagtgtctg gacaaggcga tagtttacat
     7441 gaacatattg caaatttagc tggtagccct gctattaaaa aaggtatttt acagactgta
     7501 aaagttgttg atgaattggt caaagtaatg gggcggcata agccagaaaa tatcgttatt
     7561 gaaatggcac gtgaaaatca gacaactcaa aagggccaga aaaattcgcg agagcgtatg
     7621 aaacgaatcg aagaaggtat caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt
     7681 gaaaatactc aattgcaaaa tgaaaagctc tatctctatt atctccaaaa tggaagagac
     7741 atgtatgtgg accaagaatt agatattaat cgtttaagtg attatgatgt cgatcacatt
     7801 gttccacaaa gtttccttaa agacgattca atagacaata aggtcttaac gcgttctgat
     7861 aaaaatcgtg gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac
     7921 tattggagac aacttctaaa cgccaagtta atcactcaac gtaagtttga taatttaacg
     7981 aaagctgaac gtggaggttt gagtgaactt gataaagctg gttttatcaa acgccaattg
     8041 gttgaaactc gccaaatcac taagcatgtg gcacaaattt tggatagtcg catgaatact
     8101 aaatacgatg aaaatgataa acttattcga gaggttaaag tgattacctt aaaatctaaa
     8161 ttagtttctg acttccgaaa agatttccaa ttctataaag tacgtgagat taacaattac
     8221 catcatgccc atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat taagaaatat
     8281 ccaaaacttg aatcggagtt tgtctatggt gattataaag tttatgatgt tcgtaaaatg
     8341 attgctaagt ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt ttactctaat
     8401 atcatgaact tcttcaaaac agaaattaca cttgcaaatg gagagattcg caaacgccct
     8461 ctaatcgaaa ctaatgggga aactggagaa attgtctggg ataaagggcg agattttgcc
     8521 acagtgcgca aagtattgtc catgccccaa gtcaatattg tcaagaaaac agaagtacag
     8581 acaggcggat tctccaagga gtcaatttta ccaaaaagaa attcggacaa gcttattgct
     8641 cgtaaaaaag actgggatcc aaaaaaatat ggtggttttg atagtccaac ggtagcttat
     8701 tcagtcctag tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa
     8761 gagttactag ggatcacaat tatggaaaga agttcctttg aaaaaaatcc gattgacttt
     8821 ttagaagcta aaggatataa ggaagttaaa aaagacttaa tcattaaact acctaaatat
     8881 agtctttttg agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa
     8941 aaaggaaatg agctggctct gccaagcaaa tatgtgaatt ttttatattt agctagtcat
     9001 tatgaaaagt tgaagggtag tccagaagat aacgaacaaa aacaattgtt tgtggagcag
     9061 cataagcatt atttagatga gattattgag caaatcagtg aattttctaa gcgtgttatt
     9121 ttagcagatg ccaatttaga taaagttctt agtgcatata acaaacatag agacaaacca
     9181 atacgtgaac aagcagaaaa tattattcat ttatttacgt tgacgaatct tggagctccc
     9241 gctgctttta aatattttga tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa
     9301 gttttagatg ccactcttat ccatcaatcc atcactggtc tttatgaaac acgcattgat
     9361 ttgagtcagc taggaggtga ctgactcgag
//
