LOCUS Exported 6872 bp ds-DNA circular SYN 04-MAY-2015 DEFINITION Ligation of full length mIL6 promoter with pGL2-Basic vector. ACCESSION . VERSION . KEYWORDS PIL6(1)Lrevised2TXT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6872) AUTHORS . TITLE Direct Submission JOURNAL Exported Monday, May 04, 2015 from SnapGene 2.7.2 http://www.snapgene.com COMMENT Took pGL2-Basic vector (pGL2-Luc) and digested with BglII and HindIII. Took PCR product (primers mIL6BAM and mIL6HIND, template was CH12.LX genomic DNA) and digested with BamHI and HindIII. Ligate the two together.fixed some map defects 4/24/2015 FEATURES Location/Qualifiers source 1..6872 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..1285 /note="mIL6 promoter.1" /note="full length mIL6 promoter (BamHI to HindIII)" misc_feature 1314..2966 /note="luc" /note="luciferase" misc_feature 3130..3981 /note="SV40" /note="SV40 3'-untranslated region" misc_feature 4290..4291 /note="ori" /note="ColE1-derived plasmid replication origin" misc_feature complement(5052..5912) /note="bla (amp)" /note="Beta-lactamase" misc_feature 6044..6499 /note="f1 ori" /note="direction of f1 origin of replication" ORIGIN 1 gatcctgaga gtgtgttttg taaatggttt tggattttat gtacagagcc tactttcaag 61 cctggaatca ttctgaatgc tagctagata tctggagaca ggtggacaga aaaccaggaa 121 ctagtctgaa aaagaaacta accaaaggga agaagtctgt ttaagtttga cccagcctag 181 aagacttgag cattggaggg gttattcaga gtgagacgta ccaccttcag attcaaatcc 241 tgtcatccag tagaagggag cttcaaacac aagctagcta agatacaatg aggtccttct 301 tcgatatctt tatcttccat ataccatgaa tcaaagaaac ttcaacaaca tgaggactgc 361 aacagacctt caagcctcct tgcatgacct ggaaattggg tgttttgggg tgtcgggcag 421 cagcagtggg atcagcacta acagataagg gcaactctca cagagactaa aggtcttaac 481 taagaagata gccaagagac cactggggag aatgcagaga ataggcttgg acttggaagc 541 caagattgct tgacaacaga cagaagatat ttctgtactt cacccacttt acccacctgg 601 caactcctgg aaacaactgc acaaaatttg gaggtgaaca aaccattaga aacaactggt 661 cctgacaaga cacaggaaaa acaagcaata tgcaacatta ctgtctgttg tccaggttgg 721 gtgctggggg tgggagaggg agtgtgtgtc tttgtatgat ctgaaaaaac tcaggtcaga 781 acatctgtag atccttacag acatacaaaa gaatcctagc ctcttattca tgtgtgtgtg 841 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tatgtgtgtg tcgtctgtca tgcgcgcgtg 901 cctgcgttta aataacatca gctttagctt ctctttctcc ttataaaaca ttgtgaattt 961 cagttttctt tcccatcaag acatgctcaa gtgctgagtc acttttaaag aaaaaaaaga 1021 agagtgctca tgcttcttag ggctagcctc aaggatgact taagcacact ttccccttcc 1081 tagttgtgat tctttcgatg ctaaacgacg tcacattgtg caatcttaat aaggtttcca 1141 atcagcccca cccactctgg ccccaccccc accctccaac aaagattttt atcaaatgtg 1201 ggattttccc atgagtctca aaattagaga gttgactcct aataaatatg agactgggga 1261 tgtctgtagc tcattctgct ctggaagctt ggcattccgg tactgttggt aaaatggaag 1321 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc tctagaggat ggaaccgctg 1381 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta 1441 cagatgcaca tatcgaggtg aacatcacgt acgcggaata cttcgaaatg tccgttcggt 1501 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg 1561 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg 1621 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgaac atttcgcagc 1681 ctaccgtagt gtttgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaaat 1741 taccaataat ccagaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt 1801 cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtac 1861 cagagtcctt tgatcgtgac aaaacaattg cactgataat gaattcctct ggatctactg 1921 ggttacctaa gggtgtggcc cttccgcata gaactgcctg cgtcagattc tcgcatgcca 1981 gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat 2041 tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg 2101 tcttaatgta tagatttgaa gaagagctgt ttttacgatc ccttcaggat tacaaaattc 2161 aaagtgcgtt gctagtacca accctatttt cattcttcgc caaaagcact ctgattgaca 2221 aatacgattt atctaattta cacgaaattg cttctggggg cgcacctctt tcgaaagaag 2281 tcggggaagc ggttgcaaaa cgcttccatc ttccagggat acgacaagga tatgggctca 2341 ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg 2401 gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg 2461 gcgttaatca gagaggcgaa ttatgtgtca gaggacctat gattatgtcc ggttatgtaa 2521 acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca 2581 tagcttactg ggacgaagac gaacacttct tcatagttga ccgcttgaag tctttaatta 2641 aatacaaagg atatcaggtg gcccccgctg aattggaatc gatattgtta caacacccca 2701 acatcttcga cgcgggcgtg gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg 2761 ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtgg 2821 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac 2881 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca 2941 agaagggcgg aaagtccaaa ttgtaaaatg taactgtatt cagcgatgac gaaattctta 3001 gctattgtaa tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg 3061 gtgcccttaa acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga 3121 aattcgccgg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact 3181 acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa 3241 actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga 3301 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag 3361 tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt 3421 agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag 3481 taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata 3541 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca 3601 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc 3661 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat 3721 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg 3781 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 3841 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 3901 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 3961 tatcttatca tgtctggatc cgtcgaccga tgcccttgag agccttcaac ccagtcagct 4021 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca 4081 tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg 4141 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 4201 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 4261 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 4321 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 4381 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 4441 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt 4501 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 4561 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 4621 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 4681 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 4741 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 4801 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 4861 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 4921 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 4981 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 5041 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 5101 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 5161 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 5221 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 5281 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 5341 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 5401 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 5461 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 5521 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 5581 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 5641 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 5701 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 5761 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 5821 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 5881 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 5941 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 6001 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc 6061 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 6121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 6181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 6241 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 6301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 6361 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 6421 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 6481 atattaacgt ttacaatttc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 6541 tcggtgcggg cctcttcgct attacgccag cccaagctac catgataagt aagtaatatt 6601 aaggtacgtg gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa 6661 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa 6721 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 6781 tggtttgtcc aaactcatca atgtatctta tggtactgta actgagctaa cataacccgg 6841 gaggtaccga gctcttacgc gtgctagctc ga //