LOCUS       NEWMOL        6780 bp    DNA     circular     13-JAN-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|686848864|
COMMENT     VNTDBDATE|686848864|
COMMENT     LSOWNER|
COMMENT     VNTNAME|NEWMOL|
COMMENT     VNTAUTHORNAME|BioQuant|
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COMMENT                                        " N1: 2138 " 1) 
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COMMENT                          5 "AAGCTT" "TTCGAA") 
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COMMENT                                         " N3: 3145 " 1) 
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COMMENT                                         " N4: 3654 " 1))))) "NcoI: 4 sites") 1 
COMMENT                          5 "CCATGG" "GGTACC") 
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COMMENT                                           " N1: 1287 " 1) 
COMMENT                                     #106=(CLinePar 
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COMMENT                                           " N2: 1808 " 1) 
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COMMENT                                           " N3: 2065 " 1) 
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COMMENT                                           " N4: 2137 " 1) 
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COMMENT                                           " N5: 3639 " 1))))) "PstI: 5 sites") 
COMMENT                           5 1 "CTGCAG" "GACGTC") 
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COMMENT                           3 "CCCGGG" "GGGCCC") 
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COMMENT                           5 "CCCGGG" "GGGCCC"))) "Restriction/Methylation Map") 
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COMMENT                             (CObjectList 
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COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
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COMMENT                                    "Reading Frame 1 (2 ORFs)") 
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COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
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COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
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COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
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FEATURES             Location/Qualifiers
     CDS             901..933
                     /vntifkey="4"
                     /label=PKIt\NES
     CDS             934..1635
                     /vntifkey="4"
                     /label=mCherry
     CDS             1639..1665
                     /vntifkey="4"
                     /label=GS\linker
     CDS             1666..2073
                     /vntifkey="4"
                     /label=AsLov2
     CDS             2074..2103
                     /vntifkey="4"
                     /label=cMyc1A\NLS
     promoter        236..852
                     /vntifkey="29"
                     /label=CMV
BASE COUNT     1611 a      1761 c      1772 g      1636 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 atgttagcct tgaaattagc aggtcttgat atcgtgagca agggcgagga ggataacatg 
      961 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac 
     1021 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag 
     1081 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc 
     1141 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg 
     1201 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg 
     1261 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc 
     1321 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc 
     1381 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg 
     1441 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag 
     1501 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac 
     1561 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc 
     1621 atggacgagc tgtacaaggg tggatctgga ggttcaggtg gaagtttggc tactacactt 
     1681 gaacgtattg agaagaactt tgtcattact gacccaagat tgccagataa tcccattata 
     1741 ttcgcgtccg atagtttctt gcagttgaca gaatatagcc gtgaagaaat tttgggaaga 
     1801 aactgcaggt ttctacaagg tcctgaaact gatcgcgcga cagtgagaaa aattagagat 
     1861 gccatagata accaaacaga ggtcactgtt cagctgatta attatacaaa gagtggtaaa 
     1921 aagttctgga acctctttca cttgcagcct atgcgagatc agaagggaga tgtccagtac 
     1981 tttattgggg ttcagttgga tggaactgag catgtccgag atgctgccga gagagaggga 
     2041 gtcatgctga ttaagaaaac tgcagaaaat attgccgctg ccaagagggt caagttggac 
     2101 tagctcgagc ggccgccact gtgctggata tctgcagaat tccaccacac tggactagtg 
     2161 gatccgagct cggtaccaag cttaagttta aacccgctga tcagcctcga ctgtgccttc 
     2221 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 
     2281 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 
     2341 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 
     2401 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 
     2461 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 
     2521 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 
     2581 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 
     2641 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 
     2701 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 
     2761 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 
     2821 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 
     2881 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 
     2941 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 
     3001 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 
     3061 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 
     3121 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 
     3181 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 
     3241 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg 
     3301 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac 
     3361 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat 
     3421 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat 
     3481 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt 
     3541 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg 
     3601 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat 
     3661 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga 
     3721 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat 
     3781 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag 
     3841 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc 
     3901 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg 
     3961 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct 
     4021 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg 
     4081 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac 
     4141 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga 
     4201 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc 
     4261 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag 
     4321 gaatagcacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 
     4381 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 
     4441 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc 
     4501 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 
     4561 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa 
     4621 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 
     4681 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 
     4741 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 
     4801 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 
     4861 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 
     4921 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 
     4981 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 
     5041 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 
     5101 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 
     5161 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 
     5221 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 
     5281 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 
     5341 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 
     5401 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 
     5461 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 
     5521 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag 
     5581 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
     5641 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
     5701 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
     5761 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 
     5821 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 
     5881 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 
     5941 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 
     6001 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 
     6061 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 
     6121 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 
     6181 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 
     6241 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 
     6301 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 
     6361 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 
     6421 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 
     6481 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
     6541 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
     6601 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
     6661 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
     6721 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 
//
