LOCUS       NEWMOL        6831 bp    DNA     circular     13-JAN-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|686848864|
COMMENT     VNTDBDATE|686848864|
COMMENT     LSOWNER|
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COMMENT     VNTAUTHORNAME|BioQuant|
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COMMENT                                         " N3: 3196 " 1) 
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COMMENT                                         " N4: 3705 " 1))))) "NcoI: 4 sites") 1 
COMMENT                          5 "CCATGG" "GGTACC") 
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COMMENT                                           " N1: 1287 " 1) 
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COMMENT                                           " N2: 1808 " 1) 
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COMMENT                                           " N3: 2065 " 1) 
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COMMENT                                           " N4: 2188 " 1) 
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COMMENT                           5 1 "CTGCAG" "GACGTC") 
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COMMENT                           3 "CCCGGG" "GGGCCC") 
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COMMENT                           5 "CCCGGG" "GGGCCC"))) "Restriction/Methylation Map") 
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FEATURES             Location/Qualifiers
     CDS             901..933
                     /vntifkey="4"
                     /label=PKIt\NES
     CDS             934..1635
                     /vntifkey="4"
                     /label=mCherry
     CDS             1639..1665
                     /vntifkey="4"
                     /label=GS\linker
     CDS             1666..2073
                     /vntifkey="4"
                     /label=AsLov2
     promoter        236..852
                     /vntifkey="29"
                     /label=CMV
     misc_feature    2074..2154
                     /vntifkey="21"
                     /label=biNLS10
BASE COUNT     1630 a      1780 c      1786 g      1635 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 atgttagcct tgaaattagc aggtcttgat atcgtgagca agggcgagga ggataacatg 
      961 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac 
     1021 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag 
     1081 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc 
     1141 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg 
     1201 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg 
     1261 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc 
     1321 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc 
     1381 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg 
     1441 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag 
     1501 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac 
     1561 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc 
     1621 atggacgagc tgtacaaggg tggatctgga ggttcaggtg gaagtttggc tactacactt 
     1681 gaacgtattg agaagaactt tgtcattact gacccaagat tgccagataa tcccattata 
     1741 ttcgcgtccg atagtttctt gcagttgaca gaatatagcc gtgaagaaat tttgggaaga 
     1801 aactgcaggt ttctacaagg tcctgaaact gatcgcgcga cagtgagaaa aattagagat 
     1861 gccatagata accaaacaga ggtcactgtt cagctgatta attatacaaa gagtggtaaa 
     1921 aagttctgga acctctttca cttgcagcct atgcgagatc agaagggaga tgtccagtac 
     1981 tttattgggg ttcagttgga tggaactgag catgtccgag atgctgccga gagagaggga 
     2041 gtcatgctga ttaagaaaac tgcagaaaat attgacgagg ccgcaaagag actgcccgac 
     2101 gccaacctgg ccgcagcagc cgcagccgca gcaaagaaga aaaagctgga ctagctcgag 
     2161 cggccgccac tgtgctggat atctgcagaa ttccaccaca ctggactagt ggatccgagc 
     2221 tcggtaccaa gcttaagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca 
     2281 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 
     2341 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 
     2401 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 
     2461 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 
     2521 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
     2581 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
     2641 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
     2701 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 
     2761 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
     2821 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
     2881 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
     2941 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 
     3001 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 
     3061 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 
     3121 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 
     3181 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 
     3241 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 
     3301 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gatgaaaaag 
     3361 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc 
     3421 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg 
     3481 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt 
     3541 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc 
     3601 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg 
     3661 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct 
     3721 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa 
     3781 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa 
     3841 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt 
     3901 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat 
     3961 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg 
     4021 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag 
     4081 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg 
     4141 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc 
     4201 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact 
     4261 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa 
     4321 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac 
     4381 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 
     4441 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 
     4501 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 
     4561 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 
     4621 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca 
     4681 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 
     4741 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 
     4801 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 
     4861 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 
     4921 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 
     4981 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 
     5041 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 
     5101 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 
     5161 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 
     5221 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 
     5281 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 
     5341 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 
     5401 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 
     5461 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 
     5521 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 
     5581 tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt 
     5641 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
     5701 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 
     5761 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 
     5821 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 
     5881 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 
     5941 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
     6001 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 
     6061 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 
     6121 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 
     6181 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 
     6241 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
     6301 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 
     6361 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 
     6421 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 
     6481 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 
     6541 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
     6601 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 
     6661 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 
     6721 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 
     6781 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c 
//
