LOCUS       NEWMOL        6825 bp    DNA     circular     13-JAN-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|686848864|
COMMENT     VNTDBDATE|686848864|
COMMENT     LSOWNER|
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COMMENT     VNTAUTHORNAME|BioQuant|
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COMMENT                          5 "CCATGG" "GGTACC") 
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COMMENT                                           " N1: 1287 " 1) 
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COMMENT                                           " N2: 1808 " 1) 
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COMMENT                                           " N3: 2065 " 1) 
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COMMENT                                           " N4: 2182 " 1) 
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COMMENT                           5 1 "CTGCAG" "GACGTC") 
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COMMENT                           3 "CCCGGG" "GGGCCC") 
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FEATURES             Location/Qualifiers
     CDS             901..933
                     /vntifkey="4"
                     /label=PKIt\NES
     CDS             934..1635
                     /vntifkey="4"
                     /label=mCherry
     CDS             1639..1665
                     /vntifkey="4"
                     /label=GS\linker
     CDS             1666..2073
                     /vntifkey="4"
                     /label=AsLov2
     promoter        236..852
                     /vntifkey="29"
                     /label=CMV
     misc_feature    2074..2148
                     /vntifkey="21"
                     /label=biNLS11
BASE COUNT     1630 a      1777 c      1783 g      1635 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 atgttagcct tgaaattagc aggtcttgat atcgtgagca agggcgagga ggataacatg 
      961 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac 
     1021 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag 
     1081 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc 
     1141 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg 
     1201 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg 
     1261 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc 
     1321 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc 
     1381 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg 
     1441 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag 
     1501 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac 
     1561 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc 
     1621 atggacgagc tgtacaaggg tggatctgga ggttcaggtg gaagtttggc tactacactt 
     1681 gaacgtattg agaagaactt tgtcattact gacccaagat tgccagataa tcccattata 
     1741 ttcgcgtccg atagtttctt gcagttgaca gaatatagcc gtgaagaaat tttgggaaga 
     1801 aactgcaggt ttctacaagg tcctgaaact gatcgcgcga cagtgagaaa aattagagat 
     1861 gccatagata accaaacaga ggtcactgtt cagctgatta attatacaaa gagtggtaaa 
     1921 aagttctgga acctctttca cttgcagcct atgcgagatc agaagggaga tgtccagtac 
     1981 tttattgggg ttcagttgga tggaactgag catgtccgag atgctgccga gagagaggga 
     2041 gtcatgctga ttaagaaaac tgcagaaaat attgacgagg ccgcaaagag actgcccgac 
     2101 gccaacctgg cagccgcagc caagaccaag agaaagaaac tggactagct cgagcggccg 
     2161 ccactgtgct ggatatctgc agaattccac cacactggac tagtggatcc gagctcggta 
     2221 ccaagcttaa gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc 
     2281 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 
     2341 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 
     2401 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 
     2461 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta 
     2521 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 
     2581 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 
     2641 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 
     2701 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 
     2761 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 
     2821 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 
     2881 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 
     2941 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 
     3001 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 
     3061 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 
     3121 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 
     3181 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc 
     3241 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag 
     3301 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgatgaa aaagcctgaa 
     3361 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg 
     3421 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga 
     3481 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg 
     3541 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag 
     3601 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa 
     3661 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc 
     3721 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact 
     3781 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg 
     3841 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc 
     3901 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg 
     3961 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc 
     4021 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag 
     4081 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat 
     4141 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat 
     4201 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg 
     4261 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc 
     4321 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtgcta 
     4381 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 
     4441 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 
     4501 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 
     4561 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 
     4621 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 
     4681 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 
     4741 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 
     4801 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 
     4861 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 
     4921 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 
     4981 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 
     5041 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 
     5101 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 
     5161 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 
     5221 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 
     5281 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 
     5341 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 
     5401 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 
     5461 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 
     5521 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 
     5581 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc 
     5641 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 
     5701 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 
     5761 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 
     5821 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 
     5881 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 
     5941 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 
     6001 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 
     6061 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 
     6121 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 
     6181 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 
     6241 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 
     6301 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 
     6361 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 
     6421 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 
     6481 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 
     6541 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 
     6601 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 
     6661 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 
     6721 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 
     6781 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc 
//
