LOCUS       pmCherry-N1	4722        6838 bp    DNA     circular     19-MAR-1980
DEFINITION  pmCherry-N1.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|pmCherry-N1	4722|
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COMMENT                          "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" 
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COMMENT                          "Organism(s): <a href=\"ncbilink:taxonomy/other+sequences;+artificial+sequences;+vectors.\">other sequences; artificial sequences; vectors.(other sequences; artificial sequences; vectors.)</a>" 
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FEATURES             Location/Qualifiers
     misc_feature    65..352
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        10..562
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    519..539
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        520..589
                     /vntifkey="30"
                     /label=CMV_promoter
     misc_feature    3717..3736
                     /vntifkey="21"
                     /label=EBV_rev_primer
     rep_origin      complement(3886..4192)
                     /vntifkey="33"
                     /label=f1_origin
     promoter        4271..4299
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    complement(4365..4385)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(4371..4586)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        4383..4651
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      4550..4627
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    4612..4631
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             4734..5528
                     /gene="NeoR/KanR"
                     /vntifkey="4"
                     /label=NeoR/KanR
                     /note="ORF frame 2"
     gene            4737..5525
                     /gene="NeoR/KanR"
                     /vntifkey="60"
     terminator      5703..5972
                     /vntifkey="43"
                     /label=TK_PA_terminator
     rep_origin      6120..6739
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             679..939
                     /vntifkey="4"
                     /label=LexA
     CDS             2335..3036
                     /vntifkey="4"
                     /label=mCherry
     CDS             3067..3474
                     /vntifkey="4"
                     /label=AsLOV2\wt
     CDS             958..2142
                     /vntifkey="4"
                     /label=MBP\stuffer\fragment
     CDS             2158..2292
                     /vntifkey="4"
                     /label=VP16\TA
BASE COUNT     1707 a      1772 c      1845 g      1514 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 
      661 gatccaccgg tcgccaccat gaaagcgtta acggccaggc aacaagaggt gtttgatctc 
      721 atccgtgatc acatcagcca gacaggtatg ccgccgacgc gtgcggaaat cgcgcagcgt 
      781 ttggggttcc gttccccaaa cgcggctgaa gaacatctga aggcgctggc acgcaaaggc 
      841 gttattgaaa ttgtttccgg cgcatcacgc gggattcgtc tgttgcagga agaggaagaa 
      901 gggttgccgc tggtaggtcg tgtggctgcc ggtgaaccag gtggtagcgg aaccagcaaa 
      961 ataaaaacag gtgcacgcat cctcgcatta tccgcattaa cgacgatgat gttttccgcc 
     1021 tcggctctcg ccaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc 
     1081 tataacggcc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc 
     1141 gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc 
     1201 cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg 
     1261 gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc 
     1321 gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt 
     1381 tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat 
     1441 aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc 
     1501 acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac 
     1561 gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt 
     1621 gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc 
     1681 tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac 
     1741 accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa 
     1801 ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggcg 
     1861 aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac 
     1921 aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt 
     1981 attgccgcca ccatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg 
     2041 tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact 
     2101 gtcgatgaag ccctgaaaga cgcgcagact cgtatcacca agggcggcag cggtaccgaa 
     2161 agcggcgggc cggccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc 
     2221 cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac 
     2281 atgctccccg ggactagtgg agacgccggc ggcagcggcg gcagcgtcgt ctcagtgagc 
     2341 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg 
     2401 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac 
     2461 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg 
     2521 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac 
     2581 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac 
     2641 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc 
     2701 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag 
     2761 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag 
     2821 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag 
     2881 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag 
     2941 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag 
     3001 ggccgccact ccaccggcgg catggacgag ctgtacaagg gtggatctgg aggttcaggt 
     3061 ggaagtttgg ctactacact tgaacgtatt gagaagaact ttgtcattac tgacccaaga 
     3121 ttgccagata atcccattat attcgcgtcc gatagtttct tgcagttgac agaatatagc 
     3181 cgtgaagaaa ttttgggaag aaactgcagg tttctacaag gtcctgaaac tgatcgcgcg 
     3241 acagtgagaa aaattagaga tgccatagat aaccaaacag aggtcactgt tcagctgatt 
     3301 aattatacaa agagtggtaa aaagttctgg aacctctttc acttgcagcc tatgcgagat 
     3361 cagaagggag atgtccagta ctttattggg gttcagttgg atggaactga gcatgtccga 
     3421 gatgctgccg agagagaggg agtcatgctg attaagaaaa ctgcagaaaa tattggagac 
     3481 caatagtaga aattaaaggt ctcagcggcc gcgactctag atcataatca gccataccac 
     3541 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca 
     3601 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata 
     3661 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
     3721 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa 
     3781 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 
     3841 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 
     3901 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 
     3961 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 
     4021 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 
     4081 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 
     4141 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 
     4201 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 
     4261 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 
     4321 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt 
     4381 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 
     4441 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 
     4501 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct 
     4561 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 
     4621 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 
     4681 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg 
     4741 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 
     4801 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 
     4861 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg 
     4921 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 
     4981 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 
     5041 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 
     5101 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 
     5161 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 
     5221 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg 
     5281 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 
     5341 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 
     5401 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 
     5461 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 
     5521 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc 
     5581 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg 
     5641 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc 
     5701 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac 
     5761 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt 
     5821 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc 
     5881 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc 
     5941 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat 
     6001 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 
     6061 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 
     6121 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 
     6181 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 
     6241 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta 
     6301 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 
     6361 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 
     6421 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 
     6481 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 
     6541 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 
     6601 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 
     6661 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 
     6721 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 
     6781 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat 
//
