LOCUS       pFF530                  3211 bp    DNA     circular UNA 10-OCT-2014
DEFINITION  Plasmid.
ACCESSION   urn.local...1412276277414.954
VERSION     urn.local...1412276277414.954
KEYWORDS    .
SOURCE      E. coli
  ORGANISM  E. coli
            .
FEATURES             Location/Qualifiers
     promoter        15..88
                     /created_by="User"
     misc_RNA        97..178
                     /product="msr"
                     /function="primer for the Ec86 RT"
     misc_RNA        complement(168..298)
                     /product=" msd(kanR_ON)"
                     /function="template for Ec86 RT"
                     /modified_by="User"
     cds             318..1280
                     /locus_tag="ECD_00831"
                     /codon_start=1
                     /transl_table=11
                     /product="retron EC86 RNA-directed DNA polymerase-like
                     protein"
     terminator      1305..1409
                     /created_by="User"
                     /Transferred_From="pFF530 (modified) - TermT1"
                     /Transferred_Similarity="100.00%"
     rep_origin      1552..2222
                     /note="colE1"
                     /Transferred_From="JF796094 - colE1 rep origin"
                     /Transferred_Similarity="100.00%"
     cds             complement(2351..3010)
                     /codon_start=1
                     /transl_table=11
                     /product="CmR"
ORIGIN      
        1 ctcgagatgc tagcaattgt gagcggataa caattgacat tgtgagcgga taacaagata
       61 ctgagcacat cagcaggacg cactgacctt aattaaatgc gcacccttag cgagaggttt
      121 atcattaagg tcaacctctg gatgttgttt cggcatcctg cattgaatct gagttactgt
      181 ctgttttcct gaattccgat agattgtcgc acctgattgc ccgacattat cgcgggccca
      241 tttataccca tataaatcag catccatgtt ggaattcagg aaacccgttt tttctgacgt
      301 aagggtgcgc aactttcatg aaatccgctg aatatttgaa cacttttaga ttgagaaatc
      361 tcggcctacc tgtcatgaac aatttgcatg acatgtctaa ggcgactcgc atatctgttg
      421 aaacacttcg gttgttaatc tatacagctg attttcgcta taggatctac actgtagaaa
      481 agaaaggccc agagaagaga atgagaacca tttaccaacc ttctcgagaa cttaaagcct
      541 tacaaggatg ggttctacgt aacattttag ataaactgtc gtcatctcct ttttctattg
      601 gatttgaaaa gcaccaatct attttgaata atgctacccc gcatattggg gcaaacttta
      661 tactgaatat tgatttggag gattttttcc caagtttaac tgctaacaaa gtttttggag
      721 tgttccattc tcttggttat aatcgactaa tatcttcagt tttgacaaaa atatgttgtt
      781 ataaaaatct gctaccacaa ggtgctccat catcacctaa attagctaat ctaatatgtt
      841 ctaaacttga ttatcgtatt cagggttatg caggtagtcg gggcttgata tatacgagat
      901 atgccgatga cctcacctta tctgcacagt ctatgaaaaa ggttgttaaa gcacgtgatt
      961 ttttattttc tataatccca agtgaaggat tggttattaa ctcaaaaaaa acttgtatta
     1021 gtgggcctcg tagtcagagg aaagttacag gtttagttat ttcacaagag aaagttggga
     1081 taggtagaga aaaatataaa gaaattagag caaagataca tcatatattt tgcggtaagt
     1141 cttctgagat agaacacgtt aggggatggt tgtcatttat tttaagtgtg gattcaaaaa
     1201 gccataggag attaataact tatattagca aattagaaaa aaaatatgga aagaaccctt
     1261 taaataaagc gaagacctaa ggatcccatg gtacgcgtgc tagaggcatc aaataaaacg
     1321 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1381 cctgagtagg acaaatccgc cgccctagac ctaggcgttc ggctgcggcg agcggtatca
     1441 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1501 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1561 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1621 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1681 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1741 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1801 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1861 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1921 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1981 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2041 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2101 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2161 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2221 atgactagtg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     2281 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     2341 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     2401 ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca
     2461 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     2521 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     2581 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     2641 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     2701 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     2761 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
     2821 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     2881 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     2941 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     3001 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     3061 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc
     3121 attttcgcca gatatcgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     3181 taggcgtatc acgaggccct ttcgtcttca c
//
