LOCUS       pFF714                  4024 bp    DNA     circular UNA 10-OCT-2014
DEFINITION  Plasmid.
ACCESSION   urn.local...1412276949045.1114
VERSION     urn.local...1412276949045.1114
KEYWORDS    .
SOURCE      E. coli
  ORGANISM  E. coli
            .
FEATURES             Location/Qualifiers
     promoter        15..88
                     /created_by="User"
                     /Transferred_From="pFF714 (modified) - pLlacO"
                     /Transferred_Similarity="100.00%"
     misc_rna        97..178
                     /product="msr"
                     /function="primer for the Ec86 RT"
                     /Transferred_From="pFF530 (modified) - msr86"
                     /Transferred_Similarity="100.00%"
     misc_RNA        complement(168..301)
                     /created_by="User"
                     /function="Template for Ec86 RT"
                     /Transferred_From="pFF714 (modified) - msd(KanR_ON)"
                     /Transferred_Similarity="100.00%"
                     /modified_by="User"
     cds             321..1283
                     /locus_tag="ECD_00831"
                     /codon_start=1
                     /transl_table=11
                     /product="retron EC86 RNA-directed DNA polymerase-like
                     protein"
     cds             1320..2105
                     /note="recombination;261"
                     /codon_start=1
                     /transl_table=11
                     /product="bet protein"
     terminator      2118..2222
                     /created_by="User"
                     /Transferred_From="pFF530 (modified) - TermT1"
                     /Transferred_Similarity="100.00%"
     rep_origin      2365..3035
                     /note="colE1"
                     /Transferred_From="JF796094 - colE1 rep origin"
                     /Transferred_Similarity="100.00%"
     cds             complement(3164..3823)
                     /codon_start=1
                     /transl_table=11
                     /product="CmR"
ORIGIN      
        1 ctcgagatgc tagcaattgt gagcggataa caattgacat tgtgagcgga taacaagata
       61 ctgagcacat cagcaggacg cactgacctt aattaaatgc gcacccttag cgagaggttt
      121 atcattaagg tcaacctctg gatgttgttt cggcatcctg cattgaatct gagttactgt
      181 ctgttttcct gaattcggaa actgctttgg tccccagtga gcggcaatcg attcattagg
      241 catgatcttt cttgccgagc gcggaaatta gctggaattc aggaaacccg ttttttctga
      301 cgtaagggtg cgcaactttc atgaaatccg ctgaatattt gaacactttt agattgagaa
      361 atctcggcct acctgtcatg aacaatttgc atgacatgtc taaggcgact cgcatatctg
      421 ttgaaacact tcggttgtta atctatacag ctgattttcg ctataggatc tacactgtag
      481 aaaagaaagg cccagagaag agaatgagaa ccatttacca accttctcga gaacttaaag
      541 ccttacaagg atgggttcta cgtaacattt tagataaact gtcgtcatct cctttttcta
      601 ttggatttga aaagcaccaa tctattttga ataatgctac cccgcatatt ggggcaaact
      661 ttatactgaa tattgatttg gaggattttt tcccaagttt aactgctaac aaagtttttg
      721 gagtgttcca ttctcttggt tataatcgac taatatcttc agttttgaca aaaatatgtt
      781 gttataaaaa tctgctacca caaggtgctc catcatcacc taaattagct aatctaatat
      841 gttctaaact tgattatcgt attcagggtt atgcaggtag tcggggcttg atatatacga
      901 gatatgccga tgacctcacc ttatctgcac agtctatgaa aaaggttgtt aaagcacgtg
      961 attttttatt ttctataatc ccaagtgaag gattggttat taactcaaaa aaaacttgta
     1021 ttagtgggcc tcgtagtcag aggaaagtta caggtttagt tatttcacaa gagaaagttg
     1081 ggataggtag agaaaaatat aaagaaatta gagcaaagat acatcatata ttttgcggta
     1141 agtcttctga gatagaacac gttaggggat ggttgtcatt tattttaagt gtggattcaa
     1201 aaagccatag gagattaata acttatatta gcaaattaga aaaaaaatat ggaaagaacc
     1261 ctttaaataa agcgaagacc taaggatccg gttgatattg attcagaggt ataaaacgaa
     1321 tgagtactgc actcgcaacg ctggctggga agctggctga acgtgtcggc atggattctg
     1381 tcgacccaca ggaactgatc accactcttc gccagacggc atttaaaggt gatgccagcg
     1441 atgcgcagtt catcgcatta ctgatcgttg ccaaccagta cggccttaat ccgtggacga
     1501 aagaaattta cgcctttcct gataagcaga atggcatcgt tccggtggtg ggcgttgatg
     1561 gctggtcccg catcatcaat gaaaaccagc agtttgatgg catggacttt gagcaggaca
     1621 atgaatcctg tacatgccgg atttaccgca aggaccgtaa tcatccgatc tgcgttaccg
     1681 aatggatgga tgaatgccgc cgcgaaccat tcaaaactcg cgaaggcaga gaaatcacgg
     1741 ggccgtggca gtcgcatccc aaacggatgt tacgtcataa agccatgatt cagtgtgccc
     1801 gtctggcctt cggatttgct ggtatctatg acaaggatga agccgagcgc attgtcgaaa
     1861 atactgcata cactgcagaa cgtcagccgg aacgcgacat cactccggtt aacgatgaaa
     1921 ccatgcagga gattaacact ctgctgatcg ccctggataa aacatgggat gacgacttat
     1981 tgccgctctg ttcccagata tttcgccgcg acattcgtgc atcgtcagaa ctgacacagg
     2041 ccgaagcagt aaaagctctt ggattcctga aacagaaagc cgcagagcag aaggtggcag
     2101 catgaacgcg tgctagaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     2161 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta
     2221 gacctaggcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2281 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2341 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2401 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2461 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2521 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     2581 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2641 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2701 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2761 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     2821 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2881 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2941 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3001 tggaacgaaa actcacgtta agggattttg gtcatgacta gtgcttggat tctcaccaat
     3061 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     3121 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
     3181 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga
     3241 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     3301 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     3361 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
     3421 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     3481 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     3541 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     3601 tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     3661 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     3721 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     3781 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     3841 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     3901 agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccagatatcg acgtctaaga
     3961 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     4021 tcac
//
