LOCUS       pc'ICUE3.xdna    9186 bp      DNA     circular   3/12/15
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=Degen. DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     complement(7025..7045)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(4739..4756)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(7413..8041)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(7051..7073)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(8193..8852)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             5894..6685
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(9092..9120)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(7078..7107)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    5675..5752
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      4779..5006
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA Aggatcccat ggtgagcaag 
     1021 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 
     1081 ggccacaggt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 
     1141 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 
     1201 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 
     1261 ttcaagtccg ccatgcccga aggctacgtc caggagcgta ccatcttctt caaggacgac 
     1321 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 
     1381 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 
     1441 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc 
     1501 cacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 
     1561 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 
     1621 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 
     1681 gtgaccgccg ccgggatcac tcacggcatg gacgagctgt acaaggctgg tacccccgtg 
     1741 ggaactcatg agatggagga ggagttggcc gaagctgtgg ccctgctctc ccagcggggg 
     1801 cctgacgccc tgctcactgt ggcacttcga aagcccccag gtcagcgcac ggatgaagag 
     1861 ctggacctca tctttgagga gctgctgcac atcaaggctg tggcccacct ctccaactcg 
     1921 gtgaagcgag aattagcggc tgttctgctc tttgaaccac acagcaaggc agggaccgtg 
     1981 ttgttcagcc agggggacaa gggcacttcg tggtacatta tctggaaggg atctgtcaac 
     2041 gtggtgaccc atggcaaggg gctggtgacc accctgcatg agggagatga ttttggacag 
     2101 ctggctctgg tgaatgatgc accccgggca gccaccatca tcctgcgaga agacaactgt 
     2161 catttcctgc gtgtggacaa gcaggacttc aaccgtatca tcaaggatgt ggaggcaaag 
     2221 accatgcggc tggaagaaca tggcaaagtg gtgctggtgc tggagagagc ctctcagggc 
     2281 gccggccctt cccgaccccc aaccccaggc aggaaccggt atacagtgat gtctggcacc 
     2341 ccagagaaga tcctagagct tctgttggag gccatgggac cagattccag tgctcatgac 
     2401 caaacagaga cattcctcag cgacttcctc ctgacccaca gggtcttcat gcccagcgcc 
     2461 caactctgcg ctgcccttct gcaccacttc catgtggagc ctgcgggtgg cagcgagcag 
     2521 gagcgcagca cctacgtctg caacaagagg cagcagatct tgcggctggt cagccagtgg 
     2581 gtggccctgt atggctccat gctccacact gaccctgtgg ccaccagctt cctccagaaa 
     2641 ctctcagacc tggtgggcag ggacacccga ctcagcaacc tgctgaggga gcagtggcca 
     2701 gagaggcggc gatgccacag gttggagaat ggctgtggga atgcatctcc tcagatgaag 
     2761 gcccggaact tgcctgtttg gctccccaac caggacgagc cccttcctgg cagcagctgt 
     2821 gccatccaag ttggggataa agtcccctat gacatctgcc ggccagacca ctcagtgttg 
     2881 accctgcagc tgcctgtgac agcctccgtg agagaggtga tggcagcgtt ggcccaggag 
     2941 gatggctgga ccaaggggca ggtgctggtg aaggtcaatt ctgcaggtga tgccattggc 
     3001 ctgcagccag atgcccgtgg tgtggccaca tctctggggc tcaatgagcg tctctttgtt 
     3061 gtcaacccac aggaagcgca tgagctgatc ccacaccctg accagctggg gcccactgtg 
     3121 ggctctgctg aggggctgga cctggtgagt gccaaggacc tggcaggcca gctgacggac 
     3181 cacgactgga gcctcttcaa cagtatccac caggtggagc tgatccacta tgtgctgggc 
     3241 ccccagcatc tgcgggatgt caccaccgcc aacctggagc gcttcatgcg ccgcttcaat 
     3301 gagctgcagt actgggtggc caccgagctg tgtctctgcc ccgtgcccgg cccccgggcc 
     3361 cagctgctca ggaagttcat taagctggcg gcccacctca aggagcagaa gaatctcaat 
     3421 tccttctttg ccgtcatgtt tggcctcagc aactcggcca tcagccgcct agcccacacc 
     3481 tgggagcggc tgccccacaa agtccggaag ctgtactccg ccctcgagag gctgctggac 
     3541 ccctcatgga accaccgggt ataccgactg gccctcgcca agctctcccc tcctgtcatc 
     3601 cccttcatgc cccttcttct caaagacatg accttcattc atgagggaaa ccacacacta 
     3661 gtggagaatc tcatcaactt tgagaagatg agaatgatgg ccagagccgc gcggatgctg 
     3721 caccactgcc gaagccacaa ccctgtgcct ctctcaccac tcagaagccg agtttcccac 
     3781 ctccacgagg acagccaggt ggcgaggatt tccacatgct cggagcagtc cctgagcacc 
     3841 cggagtccag ccagcacctg ggcttatgtc cagcagctga aggtcattga caaccagcgg 
     3901 gaactctccc gcctctcccg agagctggag ccagagctca tgcccgacaa ccactncctg 
     3961 agctaccagn ccaagntgag caaagacccc aacgagaagc gcgatcacat ggtcntgctg 
     4021 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggaggtacc 
     4081 ggtggatcta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 
     4141 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 
     4201 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc 
     4261 tggcccaccc tcgtgaccac cctgggctac ggccttcagt gcttcgcccg ctaccccgac 
     4321 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 
     4381 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 
     4441 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 
     4501 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag 
     4561 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg 
     4621 cagctcgccg accactacca gcagaacacc cccnnnggng acggccccgt gctgtaaGAA 
     4681 TTCTGCAGAT ATCCATCACA CTGGCGGCCG CTCGAGCATG CATCTAGAGG GCCCTATTct 
     4741 atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca 
     4801 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 
     4861 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 
     4921 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 
     4981 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 
     5041 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
     5101 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
     5161 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg 
     5221 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 
     5281 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
     5341 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
     5401 ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta 
     5461 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 
     5521 ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 
     5581 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 
     5641 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 
     5701 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 
     5761 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 
     5821 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg 
     5881 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 
     5941 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 
     6001 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 
     6061 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 
     6121 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 
     6181 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 
     6241 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 
     6301 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 
     6361 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 
     6421 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 
     6481 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 
     6541 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 
     6601 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 
     6661 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 
     6721 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 
     6781 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 
     6841 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat 
     6901 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 
     6961 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg 
     7021 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 
     7081 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 
     7141 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 
     7201 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 
     7261 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 
     7321 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 
     7381 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 
     7441 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 
     7501 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 
     7561 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 
     7621 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 
     7681 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 
     7741 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 
     7801 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 
     7861 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 
     7921 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 
     7981 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 
     8041 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 
     8101 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 
     8161 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 
     8221 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 
     8281 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 
     8341 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 
     8401 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 
     8461 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 
     8521 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 
     8581 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 
     8641 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 
     8701 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 
     8761 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 
     8821 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 
     8881 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 
     8941 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 
     9001 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 
     9061 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 
     9121 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 
     9181 gacgtc
//