LOCUS       Proinsulin-NanoL        8574 bp    DNA     circular     18-DEC-2014
DEFINITION  attB1-attB2.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|717676768|
COMMENT     VNTDBDATE|717676768|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Proinsulin-NanoLuc in pLX304|
COMMENT     VNTAUTHORNAME|Sean Burns|
COMMENT     VNTAUTHOREML|sburns@broadinstitute.org|
FEATURES             Location/Qualifiers
     misc_feature    7688..7708
                     /vntifkey="21"
                     /label=attB1
     misc_feature    complement(8567..13)
                     /vntifkey="21"
                     /label=attB2
     misc_feature    6468..6863
                     /vntifkey="21"
                     /label=Blast-R\cassette
     primer_bind     25..45
                     /vntifkey="28"
                     /label=V5\rev\primer\site
     primer_bind     475..485
                     /vntifkey="28"
                     /label=pLX-ORF-RV\(old)\primer
     primer_bind     95..105
                     /vntifkey="28"
                     /label=pLX-ORF-RV-new\primer
     misc_feature    6927..6942
                     /vntifkey="21"
                     /label=cPPT
     gene            6468..6863
                     /gene="blast"
                     /vntifkey="60"
     misc_feature    5319..5552
                     /vntifkey="21"
                     /label=RRE
     misc_feature    4765..4809
                     /vntifkey="21"
                     /label=HIV-1_psi_pack
     misc_feature    4474..4654
                     /vntifkey="21"
                     /label=HIV-1_5_LTR
     misc_feature    4474..4654
                     /vntifkey="21"
                     /label=truncHIV-1_3_LTR
     promoter        4201..4220
                     /vntifkey="30"
                     /label=T3_promoter
     promoter        4166..4184
                     /vntifkey="30"
                     /label=M13_reverse_primer
     misc_feature    4145..4167
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        4102..4131
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      3174..3793
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             2159..3019
                     /vntifkey="4"
                     /label=AmpR
     gene            2159..3019
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        2089..2117
                     /vntifkey="30"
                     /label=AmpR_promoter
     rep_origin      1590..1896
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    1424..1572
                     /vntifkey="21"
                     /label=lacZ_a
     misc_feature    complement(1424..1446)
                     /vntifkey="21"
                     /label=M13_pUC_fwd_primer
     promoter        complement(1415..1431)
                     /vntifkey="30"
                     /label=M13_forward20_primer
     promoter        complement(1387..1405)
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    1293..1312
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     rep_origin      1210..1308
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    1161..1180
                     /vntifkey="21"
                     /label=EBV_rev_primer
     terminator      1073..1192
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    812..992
                     /vntifkey="21"
                     /label=HIV-1_5_LTR
     misc_feature    812..992
                     /vntifkey="21"
                     /label=truncHIV-1_3_LTR
     misc_feature    759..811
                     /vntifkey="21"
                     /label=delta_U3
     misc_feature    742..763
                     /vntifkey="21"
                     /label=U3PPT
     misc_feature    742..757
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    101..676
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    7615..7635
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     misc_feature    7161..7448
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        7106..7658
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    8491..8493
                     /vntifkey="21"
                     /label=Added\PC2\site
     misc_feature    7714..7977
                     /vntifkey="21"
                     /label=Proinsulin\(mouse\Ins2)
     misc_feature    8494..8562
                     /vntifkey="21"
                     /label=Proinsulin\(mouse\Ins2)
     misc_feature    7978..7980
                     /vntifkey="21"
                     /label=Added\PC2\site
     misc_feature    7981..8490
                     /vntifkey="21"
                     /label=NLuc
BASE COUNT     2119 a      2119 c      2231 g      2105 t 
ORIGIN
        1 ttgtacaaag tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag 
       61 taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gattacaaaa 
      121 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 
      181 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 
      241 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 
      301 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 
      361 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 
      421 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 
      481 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc 
      541 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 
      601 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 
      661 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc 
      721 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 
      781 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 
      841 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 
      901 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 
      961 acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat 
     1021 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 
     1081 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 
     1141 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 
     1201 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 
     1261 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 
     1321 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca 
     1381 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg 
     1441 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca 
     1501 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga 
     1561 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 
     1621 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 
     1681 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 
     1741 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 
     1801 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 
     1861 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 
     1921 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 
     1981 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt 
     2041 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 
     2101 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 
     2161 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 
     2221 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 
     2281 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 
     2341 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 
     2401 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 
     2461 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 
     2521 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 
     2581 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 
     2641 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 
     2701 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 
     2761 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 
     2821 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 
     2881 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 
     2941 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 
     3001 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 
     3061 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 
     3121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 
     3181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 
     3241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 
     3301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 
     3361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 
     3421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 
     3481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 
     3541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 
     3601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 
     3661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 
     3721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 
     3781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 
     3841 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 
     3901 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 
     3961 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
     4021 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 
     4081 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 
     4141 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc 
     4201 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca 
     4261 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag 
     4321 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga 
     4381 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat 
     4441 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga 
     4501 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
     4561 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
     4621 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
     4681 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
     4741 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
     4801 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
     4861 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
     4921 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
     4981 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     5041 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     5101 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     5161 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa 
     5221 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag 
     5281 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc 
     5341 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga 
     5401 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa 
     5461 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct 
     5521 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc 
     5581 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt 
     5641 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata 
     5701 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa 
     5761 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata 
     5821 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt 
     5881 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca 
     5941 accccgaggg gacccttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc 
     6001 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc 
     6061 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg 
     6121 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga 
     6181 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg 
     6241 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag 
     6301 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg 
     6361 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc 
     6421 tccctcgttg accgaatcac cgacctctct ccccaggggg taccaccatg gccaagcctt 
     6481 tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca 
     6541 tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg 
     6601 gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg 
     6661 ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg 
     6721 gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca 
     6781 aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc 
     6841 cctctggtta tgtgtgggag ggcctgcagc tgcagtagta agaattctag atcttgagac 
     6901 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag 
     6961 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa 
     7021 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc 
     7081 ggctcgaggg ggcccggggt tattaatagt aatcaattac ggggtcatta gttcatagcc 
     7141 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 
     7201 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 
     7261 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 
     7321 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 
     7381 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 
     7441 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 
     7501 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 
     7561 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 
     7621 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactgtcg 
     7681 ggatcaacaa gtttgtacaa aaaagcaggc ttaatggccc tgtggatgcg cttcctgccc 
     7741 ctgctggccc tgctcttcct ctgggagtcc caccccaccc aggcttttgt caagcagcac 
     7801 ctttgtggtt cccacctggt ggaggctctc tacctggtgt gtggggagcg tggcttcttc 
     7861 tacacaccca tgtcccgccg tgaagtggag gacccacaag tggcacaact ggagctgggt 
     7921 ggaggcccgg gagcaggtga ccttcagacc ttggcactgg aggtggccca gcagaagagg 
     7981 gtcttcacac tcgaagattt cgttggggac tggcgacaga cagccggcta caacctggac 
     8041 caagtccttg aacagggagg tgtgtccagt ttgtttcaga atctcggggt gtccgtaact 
     8101 ccgatccaaa ggattgtcct gagcggtgaa aatgggctga agatcgacat ccatgtcatc 
     8161 atcccgtatg aaggtctgag cggcgaccaa atgggccaga tcgaaaaaat ttttaaggtg 
     8221 gtgtaccctg tggatgatca tcactttaag gtgatcctgc actatggcac actggtaatc 
     8281 gacggggtta cgccgaacat gatcgactat ttcggacggc cgtatgaagg catcgccgtg 
     8341 ttcgacggca aaaagatcac tgtaacaggg accctgtgga acggcaacaa aattatcgac 
     8401 gagcgcctga tcaaccccga cggctccctg ctgttccgag taaccatcaa cggagtgacc 
     8461 ggctggcggc tgtgcgaacg cattctggcg aagcgtggca ttgtagatca gtgctgcacc 
     8521 agcatctgct ccctctacca gctggagaac tactgcaact agtacccagc tttc 
//
