LOCUS       ColA-TRE3G-GFP-IRES-hCas9\(c3GIC9)       10740 bp    DNA     circular     23-DEC-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|718107957|
COMMENT     VNTDBDATE|718107957|
COMMENT     LSOWNER|
COMMENT     VNTNAME|ColA-TRE3G-GFP-IRES-hCas9 (c3GIC9)|
COMMENT     VNTAUTHORNAME|Luke Dow|
COMMENT     VNTOAUTHORNAME|CM|
FEATURES             Location/Qualifiers
     polyA_signal    7521..8223
                     /vntifkey="25"
                     /label=RBG\pA
     misc_feature    complement(9066..9923)
                     /vntifkey="21"
                     /label=ApR
     misc_binding    2485..3040
                     /vntifkey="20"
                     /label=IRES
     promoter        17..588
                     /vntifkey="29"
                     /label=pgk\promoter
     misc_feature    588..637
                     /vntifkey="21"
                     /label=ATG\FRT
     polyA_signal    644..1323
                     /vntifkey="25"
                     /label=SAdpA(stop)
     primer          2469..2481
                     /vntifkey="27"
                     /label=IRES_R\seq102312\clone\3
     misc_feature    3212..7312
                     /vntifkey="21"
                     /label=Misc_Feature_2
     misc_feature    7313..7360
                     /vntifkey="21"
                     /label=Misc_Feature_4
     misc_feature    3092..3160
                     /vntifkey="21"
                     /label=FLAG\tag
     misc_feature    3161..3211
                     /vntifkey="21"
                     /label=Misc_Feature_8
     promoter        1360..1735
                     /vntifkey="29"
                     /label=TRE3G
     misc_feature    1316..1332
                     /vntifkey="21"
                     /label=Unknown\short\sequence
     CDS             1759..2478
                     /vntifkey="4"
                     /label=GFP
     primer          2419..3338
                     /vntifkey="27"
                     /label=MGF2_seq\122314
     primer          1212..1891
                     /vntifkey="27"
                     /label=435F_seq\122314
     primer          6529..7415
                     /vntifkey="27"
                     /label=Rev45_seq\122314
     primer          1944..2857
                     /vntifkey="27"
                     /label=1275F_seq\122314
BASE COUNT     2834 a      2827 c      2763 g      2316 t 
ORIGIN
        1 gatcccccgg gctgcaggaa ttaattcgtc gacctcgaaa ttctaccggg taggggaggc 
       61 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta 
      121 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc 
      181 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc 
      241 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct 
      301 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca 
      361 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg 
      421 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc 
      481 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg 
      541 cctttcgacc tgcatccatc tagatctcga tcgagcagct gaagcttatg gagaagttac 
      601 tattccgaag ttcctattct ctagaaagta taggaacttc gatcccccgg gctgcagatc 
      661 tgtagggcgc agtagtccag ggtttccttg atgatgtcat acttatcctg tccctttttt 
      721 ttccacagct cgcggttgag gacaaactct tcgcggtctt tccagtgggg atcgacggta 
      781 tcgataagct tgcagatctg cgactctaga ggatctgcga ctctagagga tcataatcag 
      841 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 
      901 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 
      961 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 
     1021 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct gcgactctag 
     1081 aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca 
     1141 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt 
     1201 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
     1261 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 
     1321 atcgagttta ccatgcatgt gttcgattct agattcgagt ttactcccta tcagtgatag 
     1381 agaacgtatg aagagtttac tccctatcag tgatagagaa cgtatgcaga ctttactccc 
     1441 tatcagtgat agagaacgta taaggagttt actccctatc agtgatagag aacgtatgac 
     1501 cagtttactc cctatcagtg atagagaacg tatctacagt ttactcccta tcagtgatag 
     1561 agaacgtata tccagtttac tccctatcag tgatagagaa cgtataagct ttaggcgtgt 
     1621 acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc ctggagcaat 
     1681 tccacaacac ttttgtctta taccaacttt ccgtaccact tcctaccctc gtaaagtcga 
     1741 gcttgcgttg gatccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 
     1801 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 
     1861 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 
     1921 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc 
     1981 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 
     2041 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 
     2101 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 
     2161 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 
     2221 gccgacaagc agaagaacgg catcaaggcg aacttcaagg tccgccacaa catcgaggac 
     2281 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 
     2341 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag 
     2401 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 
     2461 gacgagctgt acaagtaatg aattttccgc cccccccccc taacgttact ggccgaagcc 
     2521 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt 
     2581 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc 
     2641 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc 
     2701 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc 
     2761 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg 
     2821 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct 
     2881 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat 
     2941 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct 
     3001 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggccc 
     3061 tcgagtattg atcagtggat ccggtgccac catggactat aaggaccacg acggagacta 
     3121 caaggatcat gatattgatt acaaagacga tgacgataag atggccccaa agaagaagcg 
     3181 gaaggtcggt atccacggag tcccagcagc cgacaagaag tacagcatcg gcctggacat 
     3241 cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa 
     3301 attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct 
     3361 gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag 
     3421 atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc 
     3481 caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa 
     3541 gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa 
     3601 gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct 
     3661 gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga 
     3721 gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac 
     3781 ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat 
     3841 cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg 
     3901 cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc tgacccccaa 
     3961 cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta 
     4021 cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct 
     4081 ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga 
     4141 gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga 
     4201 cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt 
     4261 cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca gccaggaaga 
     4321 gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt 
     4381 gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc 
     4441 ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc 
     4501 attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta 
     4561 cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga 
     4621 aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg cccagagctt 
     4681 catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca 
     4741 cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga aatacgtgac 
     4801 cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct 
     4861 gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa 
     4921 aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct 
     4981 gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga 
     5041 aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat 
     5101 gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct 
     5161 gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg 
     5221 ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag 
     5281 aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc 
     5341 ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc 
     5401 cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat 
     5461 gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca 
     5521 gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct 
     5581 gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct 
     5641 gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa 
     5701 ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga aggacgactc 
     5761 catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc 
     5821 ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct 
     5881 gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact 
     5941 ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt 
     6001 ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg 
     6061 ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca 
     6121 gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc 
     6181 cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg 
     6241 cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa 
     6301 ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac 
     6361 cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga 
     6421 gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca 
     6481 agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct 
     6541 gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta 
     6601 cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa 
     6661 gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag 
     6721 aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa 
     6781 aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa 
     6841 gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa 
     6901 atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga 
     6961 taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga 
     7021 gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct 
     7081 gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca 
     7141 cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat 
     7201 cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag 
     7261 catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acaaaaggcc 
     7321 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag taagaattcg ccagcacagt 
     7381 gctcgagcgt tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg 
     7441 ctattgtaaa attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg 
     7501 gaagatgtcc cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac 
     7561 tctgttgaca accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa 
     7621 actttagctt gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta 
     7681 agtactttct ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag 
     7741 cacagtttta gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa 
     7801 attctggctg gcgtggaaat attcttattg gtagaaacaa ctacaccctg gtcatcatcc 
     7861 tgcctttctc tttatggtta caatgatata cactgtttga gatgaggata aaatactctg 
     7921 agtccaaacc gggcccctct gctaaccatg ttcatgcctt cttctctttc ctacagctcc 
     7981 tgggcaacgt gctggttgtt gtgctgtctc atcattttgg caaaggattc actcctcagg 
     8041 tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc 
     8101 actgagatcg ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc 
     8161 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aatttctcga 
     8221 tcgctagcat cctaggcgcg ccacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     8281 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     8341 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     8401 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     8461 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
     8521 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     8581 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     8641 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     8701 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 
     8761 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     8821 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     8881 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     8941 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     9001 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     9061 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     9121 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     9181 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     9241 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     9301 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     9361 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     9421 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     9481 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     9541 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     9601 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     9661 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
     9721 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     9781 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     9841 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     9901 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     9961 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
    10021 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa 
    10081 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 
    10141 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 
    10201 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 
    10261 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 
    10321 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 
    10381 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 
    10441 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 
    10501 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 
    10561 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 
    10621 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat 
    10681 acgactcact atagggcgaa ttggagctcc accgcggtgg cggccgctct agaactagtg 
//
