LOCUS       ColA-TRE3G-GFP-IRES-hCas9n\(c3GIC9n)       10668 bp    DNA     circular     23-DEC-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|718108903|
COMMENT     VNTDBDATE|718109225|
COMMENT     LSOWNER|
COMMENT     VNTNAME|ColA-TRE3G-GFP-IRES-hCas9n (c3GIC9n)|
COMMENT     VNTAUTHORNAME|Luke Dow|
COMMENT     VNTOAUTHORNAME|CM|
FEATURES             Location/Qualifiers
     polyA_signal    7449..8151
                     /vntifkey="25"
                     /label=RBG\pA
     misc_feature    complement(8994..9851)
                     /vntifkey="21"
                     /label=ApR
     misc_binding    2485..3040
                     /vntifkey="20"
                     /label=IRES
     promoter        17..588
                     /vntifkey="29"
                     /label=pgk\promoter
     misc_feature    588..637
                     /vntifkey="21"
                     /label=ATG\FRT
     misc_feature    3155..7255
                     /vntifkey="21"
                     /label=hCas9n\(D10A\variant)
     misc_feature    3179..3181
                     /vntifkey="21"
                     /label=Misc_Feature_4
     misc_feature    7256..7288
                     /vntifkey="21"
                     /label=Misc_Feature_5
     misc_feature    3092..3121
                     /vntifkey="21"
                     /label=Misc_Feature_8
     misc_feature    3122..3154
                     /vntifkey="21"
                     /label=Misc_Feature_9
     promoter        1360..1735
                     /vntifkey="29"
                     /label=TRE3G
     polyA_signal    644..1312
                     /vntifkey="25"
                     /label=SAdpA\(stop)
     CDS             1759..2481
                     /vntifkey="4"
                     /label=GFP
     primer          1136..2089
                     /vntifkey="27"
                     /label=435F_seq\122314
     primer          1936..2785
                     /vntifkey="27"
                     /label=1275F_seq\122314
     primer          660..1614
                     /vntifkey="27"
                     /label=6115F_seq\122314
     primer          6346..7359
                     /vntifkey="27"
                     /label=Rev45_seq\122314
     primer          2419..3413
                     /vntifkey="27"
                     /label=MGF2_seq\122314
BASE COUNT     2800 a      2815 c      2741 g      2312 t 
ORIGIN
        1 gatcccccgg gctgcaggaa ttaattcgtc gacctcgaaa ttctaccggg taggggaggc 
       61 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta 
      121 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc 
      181 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc 
      241 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct 
      301 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca 
      361 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg 
      421 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc 
      481 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg 
      541 cctttcgacc tgcatccatc tagatctcga tcgagcagct gaagcttatg gagaagttac 
      601 tattccgaag ttcctattct ctagaaagta taggaacttc gatcccccgg gctgcagatc 
      661 tgtagggcgc agtagtccag ggtttccttg atgatgtcat acttatcctg tccctttttt 
      721 ttccacagct cgcggttgag gacaaactct tcgcggtctt tccagtgggg atcgacggta 
      781 tcgataagct tgcagatctg cgactctaga ggatctgcga ctctagagga tcataatcag 
      841 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 
      901 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 
      961 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 
     1021 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct gcgactctag 
     1081 aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca 
     1141 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt 
     1201 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
     1261 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 
     1321 atcgagttta ccatgcatgt gttcgattct agattcgagt ttactcccta tcagtgatag 
     1381 agaacgtatg aagagtttac tccctatcag tgatagagaa cgtatgcaga ctttactccc 
     1441 tatcagtgat agagaacgta taaggagttt actccctatc agtgatagag aacgtatgac 
     1501 cagtttactc cctatcagtg atagagaacg tatctacagt ttactcccta tcagtgatag 
     1561 agaacgtata tccagtttac tccctatcag tgatagagaa cgtataagct ttaggcgtgt 
     1621 acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc ctggagcaat 
     1681 tccacaacac ttttgtctta taccaacttt ccgtaccact tcctaccctc gtaaagtcga 
     1741 gcttgcgttg gatccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 
     1801 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc tggcgagggc 
     1861 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 
     1921 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc 
     1981 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 
     2041 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 
     2101 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 
     2161 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 
     2221 gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa catcgaggac 
     2281 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 
     2341 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag 
     2401 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 
     2461 gacgagctgt acaagtaatg aattttccgc cccccccccc taacgttact ggccgaagcc 
     2521 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt 
     2581 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc 
     2641 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc 
     2701 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc 
     2761 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg 
     2821 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct 
     2881 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat 
     2941 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct 
     3001 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggccc 
     3061 tcgagtattg atcagtggat ccggtgccac catgtaccca tacgatgttc cagattacgc 
     3121 ttcgccgaag aaaaagcgca aggtcgaagc gtccgacaag aagtacagca tcggcctggc 
     3181 catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa 
     3241 gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc 
     3301 cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag 
     3361 aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat 
     3421 ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga 
     3481 taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga 
     3541 gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga 
     3601 cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat 
     3661 cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca 
     3721 gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc 
     3781 catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc 
     3841 cggcgagaag aagaatggcc tgttcggcaa cctgattgcc ctgagcctgg gcctgacccc 
     3901 caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac 
     3961 ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt 
     4021 tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac 
     4081 cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca 
     4141 ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat 
     4201 tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga 
     4261 agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct 
     4321 cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat 
     4381 cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta 
     4441 cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta 
     4501 ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga 
     4561 ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag 
     4621 cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa 
     4681 gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt 
     4741 gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga 
     4801 cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa 
     4861 gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc 
     4921 cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga 
     4981 ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga 
     5041 gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca 
     5101 gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat 
     5161 ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa 
     5221 cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa 
     5281 agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag 
     5341 ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt 
     5401 gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac 
     5461 ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga 
     5521 gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa 
     5581 gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat 
     5641 caaccggctg tccgactacg atgtggacca tatcgtgcct cagagctttc tgaaggacga 
     5701 ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt 
     5761 gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa 
     5821 gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga 
     5881 actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca 
     5941 cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat 
     6001 ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt 
     6061 ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa 
     6121 cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta 
     6181 cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg 
     6241 caaggctacc gccaagtact tcttctacag caacatcatg aactttttca agaccgagat 
     6301 taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg 
     6361 ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc 
     6421 ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat 
     6481 cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa 
     6541 gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga 
     6601 aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga 
     6661 aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt 
     6721 gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg 
     6781 gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc 
     6841 caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga 
     6901 ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat 
     6961 cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt 
     7021 gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat 
     7081 ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac 
     7141 catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca 
     7201 gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag gcgacagccc 
     7261 caagaagaag agaaaggtgg aggccagcta agaattcgcc agcacagtgc tcgagcgttg 
     7321 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat 
     7381 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct 
     7441 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac 
     7501 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc 
     7561 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct 
     7621 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga 
     7681 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc 
     7741 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt 
     7801 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg 
     7861 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc 
     7921 tggttgttgt gctgtctcat cattttggca aaggattcac tcctcaggtg caggctgcct 
     7981 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcgtt 
     8041 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct 
     8101 aataaaggaa atttattttc attgcaatag tgtgttggaa tttctcgatc gctagcatcc 
     8161 taggcgcgcc acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     8221 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     8281 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     8341 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     8401 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 
     8461 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     8521 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     8581 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     8641 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 
     8701 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
     8761 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 
     8821 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 
     8881 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 
     8941 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 
     9001 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 
     9061 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 
     9121 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 
     9181 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 
     9241 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 
     9301 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 
     9361 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 
     9421 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 
     9481 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 
     9541 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 
     9601 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 
     9661 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 
     9721 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 
     9781 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 
     9841 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 
     9901 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 
     9961 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa 
    10021 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 
    10081 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 
    10141 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 
    10201 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 
    10261 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 
    10321 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 
    10381 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc 
    10441 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
    10501 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
    10561 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat 
    10621 agggcgaatt ggagctccac cgcggtggcg gccgctctag aactagtg 
//
