LOCUS pBSK?B-CAG-MCP-tdiRFP670-IRES-Neo.xdna 9280 bp DNA circular DEFINITION ACCESSION VERSION SOURCE ORGANISM FEATURES Location/Qualifiers primer_bind 2172..2191 /label=T3 primer_bind complement(8665..8682) /label=M13-fwd primer_bind 2134..2154 /label=M13-rev primer_bind complement(8636..8655) /label=T7 primer_bind 3988..4007 /label=T7 rep_origin 1138..1766 /label=ColE1 origin rep_origin complement(8828..9268) /label=F1 ori CDS 8753..8821 /label=LacZ alpha misc_binding 2106..2128 /label=LacO Promoter 59..87 /label=Amp prom Promoter 2072..2101 /label=lac intron 6526..6821 /label=synth int misc_feature 8436..8486 /label=SV40 early mRNA polyadenylation signal CDS 4058..4078 /label=NLS promoter 2854..3938 /label=CAG promoter CDS 4139..4492 /label=MCP CDS 4526..6460 /label=tdiRFP670 CDS 4082..4108 /label=HA tag CDS 4109..4120 /label=FactorXa site misc_feature 4058..4081 /label=SV40 NLS1 misc_feature 6870..7452 /label=internal ribosome entry site IRES misc_feature 7473..8276 /label=neoR KanR ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGCAGCCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG 1861 AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG 1921 AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT 1981 GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG 2041 TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT 2101 TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG 2161 CCAAGCGCGC AATTAACCCT CACTAAAGGG AACAAAAGCT GGAGCTCCAC CGCGGTGGCG 2221 GCCGCTCTAG AACTAGTGAG ACGTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA 2281 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC 2341 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG 2401 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC 2461 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG 2521 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG 2581 TATTAGTCAT CGCTATTACC ATGGGTCGAG GTGAGCCCCA CGTTCTGCTT CACTCTCCCC 2641 ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA TTTTTTAATT ATTTTGTGCA 2701 GCGATGGGGG CGGGGGGGGG GGGGGCGCGC GCCAGGCGGG GCGGGGCGGG GCGAGGGGCG 2761 GGGCGGGGCG AGGCGGAGAG GTGCGGCGGC AGCCAATCAG AGCGGCGCGC TCCGAAAGTT 2821 TCCTTTTATG GCGAGGCGGC GGCGGCGGCG GCCCTATAAA AAGCGAAGCG CGCGGCGGGC 2881 GGGAGTCGCT GCGTTGCCTT CGCCCCGTGC CCCGCTCCGC GCCGCCTCGC GCCGCCCGCC 2941 CCGGCTCTGA CTGACCGCGT TACTCCCACA GGTGAGCGGG CGGGACGGCC CTTCTCCTCC 3001 GGGCTGTAAT TAGCGCTTGG TTTAATGACG GCTCGTTTCT TTTCTGTGGC TGCGTGAAAG 3061 CCTTAAAGGG CTCCGGGAGG GCCCTTTGTG CGGGGGGGAG CGGCTCGGGG GGTGCGTGCG 3121 TGTGTGTGTG CGTGGGGAGC GCCGCGTGCG GCCCGCGCTG CCCGGCGGCT GTGAGCGCTG 3181 CGGGCGCGGC GCGGGGCTTT GTGCGCTCCG CGTGTGCGCG AGGGGAGCGC GGCCGGGGGC 3241 GGTGCCCCGC GGTGCGGGGG GGCTGCGAGG GGAACAAAGG CTGCGTGCGG GGTGTGTGCG 3301 TGGGGGGGTG AGCAGGGGGT GTGGGCGCGG CGGTCGGGCT GTAACCCCCC CCTGCACCCC 3361 CCTCCCCGAG TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTGC GGGGCGTGGC 3421 GCGGGGCTCG CCGTGCCGGG CGGGGGGTGG CGGCAGGTGG GGGTGCCGGG CGGGGCGGGG 3481 CCGCCTCGGG CCGGGGAGGG CTCGGGGGAG GGGCGCGGCG GCCCCGGAGC GCCGGCGGCT 3541 GTCGAGGCGC GGCGAGCCGC AGCCATTGCC TTTTATGGTA ATCGTGCGAG AGGGCGCAGG 3601 GACTTCCTTT GTCCCAAATC TGGCGGAGCC GAAATCTGGG AGGCGCCGCC GCACCCCCTC 3661 TAGCGGGCGC GGGCGAAGCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT 3721 CGTGCGTCGC CGCGCCGCCG TCCCCTTCTC CATCTCCAGC CTCGGGGCTG CCGCAGGGGG 3781 ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG 3841 GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA 3901 ACGTGCTGGT TGTTGTGCTG TCTCATCATT TTGGCAAAGA GCTCTCTGGC TAACTAGAGA 3961 ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTTG 4021 GTATCGACCT CGGATCGTCG CTAGCGATAT CATGGGCCCA AAAAAGAAAA GAAAAGTTGG 4081 CTACCCCTAC GACGTGCCCG ACTACGCCAT CGAAGGCCGC CATATGCTAG CCGTTAAAAT 4141 GGCTTCTAAC TTTACTCAGT TCGTTCTCGT CGACAATGGC GGAACTGGCG ACGTGACTGT 4201 CGCCCCAAGC AACTTCGCTA ACGGGATCGC TGAATGGATC AGCTCTAACT CGCGTTCACA 4261 GGCTTACAAA GTAACCTGTA GCGTTCGTCA GAGCTCTGCG CAGAATCGCA AATACACCAT 4321 CAAAGTCGAG GTGCCTAAAG GCGCCTGGCG TTCGTACTTA AATATGGAAC TAACCATTCC 4381 AATTTTCGCC ACGAATTCCG ACTGCGAGCT TATTGTTAAG GCAATGCAAG GTCTCCTAAA 4441 AGATGGAAAC CCGATTCCCT CAGCAATCGC AGCAAACTCC GGCATCTACG CCATGGCTTC 4501 TAACTTTACT CAGACCGGTG AATTCATGGC CAGAAAAGTG GACCTGACCA GTTGTGACCG 4561 AGAGCCGATA CACATTCCTG GCTCTATCCA GCCATGTGGG TGTTTGCTGG CCTGCGATGC 4621 CCAGGCAGTC AGGATTACTC GAATTACCGA AAATGCGGGT GCCTTCTTTG GCCGTGAAAC 4681 GCCCCGTGTC GGCGAACTCT TAGCCGATTA TTTCGGAGAA ACCGAGGCTC ACGCACTTAG 4741 GAACGCACTG GCGCAATCAA GCGATCCCAA ACGCCCTGCT CTCATCTTTG GGTGGAGAGA 4801 TGGACTGACA GGCCGTACTT TCGACATAAG CCTTCACCGG CATGACGGAA CCAGTATAAT 4861 TGAGTTTGAA CCGGCCGCCG CTGAGCAAGC AGACAACCCC CTTCGCCTGA CCAGACAAAT 4921 TATCGCCCGC ACTAAGGAGC TGAAATCGCT GGAAGAAATG GCCGCTCGTG TTCCCAGGTA 4981 TCTTCAGGCA ATGCTGGGTT ATCATAGGGT AATGCTCTAC AGATTCGCCG ACGATGGCTC 5041 TGGTATGGTA ATAGGGGAGG CCAAGCGATC CGACCTGGAG TCTTTTCTGG GGCAGCATTT 5101 CCCCGCATCT CTAGTACCCC AGCAGGCAAG GCTTTTGTAC CTCAAGAATG CCATCCGCGT 5161 GGTATCGGAC TCCCGCGGTA TTAGTAGCAG GATCGTTCCA GAACACGACG CCTCTGGAGC 5221 TGCGCTGGAT CTGTCTTTCG CTCACCTCCG ATCTATCAGC CCTTGTCATT TAGAGTTTCT 5281 GCGAAATATG GGGGTCAGCG CAAGTATGTC CCTGAGCATA ATAATCGATG GAACACTATG 5341 GGGCCTAATA ATTTGCCATC ATTACGAACC TAGAGCAGTA CCAATGGCAC AGCGAGTGGC 5401 CGCCGAAATG TTCGCCGATT TTTTGTCTCT TCATTTTACT GCCGCTCACC ATCAGCGCGG 5461 CCACGGGACT GGCTCGACAG GTTCTGGCAG TAGTGGGACT GCTTCATCAG AAGATAATAA 5521 CATGGCCATG GCCAGAAAAG TGGACCTGAC CAGTTGTGAC CGAGAGCCGA TACACATTCC 5581 TGGCTCTATC CAGCCATGTG GGTGTTTGCT GGCCTGCGAT GCCCAGGCAG TCAGGATTAC 5641 TCGAATTACC GAAAATGCGG GTGCCTTCTT TGGCCGTGAA ACGCCCCGTG TCGGCGAACT 5701 CTTAGCCGAT TATTTCGGAG AAACCGAGGC TCACGCACTT AGGAACGCAC TGGCGCAATC 5761 AAGCGATCCC AAACGCCCTG CTCTCATCTT TGGGTGGAGA GATGGACTGA CAGGCCGTAC 5821 TTTCGACATA AGCCTTCACC GGCATGACGG AACCAGTATA ATTGAGTTTG AACCGGCCGC 5881 CGCTGAGCAA GCAGACAACC CCCTTCGCCT GACCAGACAA ATTATCGCCC GCACTAAGGA 5941 GCTGAAATCG CTGGAAGAAA TGGCCGCTCG TGTTCCCAGG TATCTTCAGG CAATGCTGGG 6001 TTATCATAGG GTAATGCTCT ACAGATTCGC CGACGATGGC TCTGGTATGG TAATAGGGGA 6061 GGCCAAGCGA TCCGACCTGG AGTCTTTTCT GGGGCAGCAT TTCCCCGCAT CTCTAGTACC 6121 CCAGCAGGCA AGGCTTTTGT ACCTCAAGAA TGCCATCCGC GTGGTATCGG ACTCCCGCGG 6181 TATTAGTAGC AGGATCGTTC CAGAACACGA CGCCTCTGGA GCTGCGCTGG ATCTGTCTTT 6241 CGCTCACCTC CGATCTATCA GCCCTTGTCA TTTAGAGTTT CTGCGAAATA TGGGGGTCAG 6301 CGCAAGTATG TCCCTGAGCA TAATAATCGA TGGAACACTA TGGGGCCTAA TAATTTGCCA 6361 TCATTACGAA CCTAGAGCAG TACCAATGGC ACAGCGAGTG GCCGCCGAAA TGTTCGCCGA 6421 TTTTTTGTCT CTTCATTTTA CTGCCGCTCA CCATCAGCGC GGATCCGCGT TTAAACTCGA 6481 GGTTAATTAA TGAGCGGCCG CATAGATAAC TGATCCAGTG TGCTGGAATT AATTCGCTGT 6541 CTGCGAGGGC CAGCTGTTGG GGTGAGTACT CCCTCTCAAA AGCGGGCATG ACTTCTGCGC 6601 TAAGATTGTC AGTTTCCAAA AACGAGGAGG ATTTGATATT CACCTGGCCC GCGGTGATGC 6661 CTTTGAGGGT GGCCGCGTCC ATCTGGTCAG AAAAGACAAT CTTTTTGTTG TCAAGCTTGA 6721 GGTGTGGCAG GCTTGAGATC TGGCCATACA CTTGAGTGAC AATGACATCC ACTTTGCCTT 6781 TCTCTCCACA GGTGTCCACT CCCAGGTCCA ACTGCAGGTC GAGCATGCAT CTAGGGCGGC 6841 CAATTCCGCC CCTCTCCCCC CCCCCCTTTT CCCTCCCCCC CCCCTAACGT TACTGGCCGA 6901 AGCCGCTTGG AATAAGGCCG GTGTGCGTTT GTCTATATGT TATTTTCCAC CATATTGCCG 6961 TCTTTTGGCA ATGTGAGGGC CCGGAAACCT GGCCCTGTCT TCTTGACGAG CATTCCTAGG 7021 GGTCTTTCCC CTCTCGCCAA AGGAATGCAA GGTCTGTTGA ATGTCGTGAA GGAAGCAGTT 7081 CCTCTGGAAG CTTCTTGAAG ACAAACAACG TCTGTAGCGA CCCTTTGCAG GCAGCGGAAC 7141 CCCCCACCTG GCGACAGGTG CCTCTGCGGC CAAAAGCCAC GTGTATAAGA TACACCTGCA 7201 AAGGCGGCAC AACCCCAGTG CCACGTTGTG AGTTGGATAG TTGTGGAAAG AGTCAAATGG 7261 CTCTCCTCAA GCGTATTCAA CAAGGGGCTG AAGGATGCCC AGAAGGTACC CCATTGTATG 7321 GGATCTGATC TGGGGCCTCG GTGCACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAA 7381 CGTCTAGGCC CCCCGAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAAGCT 7441 TGCCACAACC CGGGATAATT CCTGCAGCCA ATATGGGATC GGCCATTGAA CAAGATGGAT 7501 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC 7561 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC 7621 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC 7681 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG 7741 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC 7801 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG 7861 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC 7921 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC 7981 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGATGAT CTCGTCGTGA 8041 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA 8101 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG 8161 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG 8221 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGGGG 8281 ATCAATTCTC TAGATAACTG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG 8341 CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG 8401 TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 8461 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 8521 TATCTTAACG CGTCGAGTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA 8581 TCATGTCTGT ATACCCGTCT CGTCGACCTC GAGGGGGGGC CCGGTACCCA ATTCGCCCTA 8641 TAGTGAGTCG TATTACGCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA 8701 CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA 8761 TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG 8821 GGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC 8881 CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC 8941 CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT 9001 TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG 9061 GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG 9121 TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT 9181 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT 9241 TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG //